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101.
Previously, we prepared two different monoclonal antibodies (mAbs) against human 4-1BB (CD137): an agonistic mAb BBK-1 and an antagonistic mAb BBK-2. In this paper, we describe the molecular cloning of these two mAbs and present comparisons of their amino acid sequences. cDNAs encoding the heavy (H) and light (L) chains of the two mAbs were cloned by screening of cDNA libraries constructed from hybridomas secreting these mAbs. Comparisons of amino acid sequences of the two mAbs showed that, while the constant regions of the H and L chains were identical between the two mAbs, the variable region showed 45% identity in H chains and 48% identity in L chains. This suggests that these two mAbs recognize different epitopes of 4-1BB and may have different effects on the activity of 4-1BB. 相似文献
102.
1983年淄博市首次发现肾综合征出血热(HFRS),1986年疫情达历史高峰;之后疫情总体呈下降趋势,现将近20年来淄博市HFRS的流行情况分析如下。1.资料与方法:人间疫情资料来源于各区县疫情报告统计,人口资料来源于淄博市统计局统计年鉴,数据用Excel 2000软件分析。2.结果: (1)流行趋势:①1983-2003年全省共发病15 507例,年均发病率为22.13/10万;病死116例,病死率为0.56%;发病率波动在0.27/10万~76.51/10万之间。20世纪80年代发 相似文献
103.
104.
P Tugwell L Hart G Kraag A Park C Dok F Bianchi C Goldsmith W W Buchanan 《The Journal of rheumatology》1984,11(4):457-461
A crossover double-blind controlled trial was performed on 36 patients with rheumatoid arthritis to assess the necessity for serum salicylate monitoring in determining optimal dosage. There was no clinically or statistically significant increase in the clinical improvement of patients associated with serum monitoring but potentially toxic serum levels occurred without tinnitus when serum monitoring was not used. 相似文献
105.
目的:探讨EB病毒感染与人大肠癌发生的关系。方法:用原位分子杂交法对130例人大肠癌标本中EB病毒小分子RNA片段进行检测。结果:130例标本中有6例(4.48%)癌组织呈阳性反应,其中4例为男性,4例有明显淋巴细胞浸润。结论:EB病毒感染可能与我国部分大肠腺癌的发生有关,肿瘤细胞间质中大量淋巴细胞浸润可能是EB病毒感染的重要病理学特征。 相似文献
106.
目的:观察肝癌形成过程中明胶酶表达(蛋白及mRNA水平)的动态变化,探索明胶酶在肝癌生长浸润中的作用。方法:应用免疫组化法、明胶酶谱法和RT-PCR法对大鼠肝癌形成过程中各期明胶酶表达情况进行观察,并与正常对照组进行比较分析。结果:正常肝组织及肝硬化组织内明胶酶有表达,肝癌形成后主要在癌细胞内表达;明胶酶原在正常肝组织中有低表达,诱癌过程中呈持续增高趋势;明胶酶-2mRNA与其酶蛋白表达趋向一致。结论:在肝癌形成过程中明胶酶转录和翻译水平均呈持续增高趋势。 相似文献
107.
Changes in outcome with sphincter preserving surgery for rectal cancer in Korea, 1991-2000. 总被引:3,自引:0,他引:3
S-B Lim S C Heo M R Lee S-B Kang Y J Park K J Park H S Choi S-Y Jeong J-G Park 《European journal of surgical oncology》2005,31(3):242-249
AIM: To report the clinical and oncological data of patients operated on for rectal cancers 3-5 cm from the AV over a 10 year period, including the Sphincter preservation (SP) rate. METHODS: We reviewed medical records of 304 patients with rectal cancers 3-5 cm from the AV who underwent surgical resection from January 1991 through December 2000. The 10 years were divided into three periods based on the introduction of new surgical techniques, specifically, ultralow anterior resection (ULAR) with double stapling in March 1994 and ULAR with coloanal anastomosis in April 1997. The rates of SP, complications and patient survival during these periods were compared. RESULTS: The SP rate increased significantly over the 10 years, from 16.4% in period I (January 1991-February 1994), to 53.0% in period II (March 1994-March 1997), to 86.5% in period III (April 1997-December 2000) (p<0.001). Over time, the age of the patients increased (p=0.004), the length of the distal resection margin became shorter (p=0.005), and the rate of lymph node metastasis increased (p=0.016). The factors significantly influencing SP were the period (p<0.001) and the distance from the AV (p<0.001). Over time, morbidity did not increase, and overall and disease free survival rates did not decrease. In contrast, the overall survival of N2 cases significantly increased over time (p=0.0492). CONCLUSION: Over 10 years, the SP rate in rectal cancers 3-5 cm from the AV was significantly increased by the introduction of the double stapling and coloanal anastomosis techniques. These surgical methods, however, had no effect on morbidity, disease free survival and overall survival rates. 相似文献
108.
109.
细胞色素氧化酶是真核生物细胞呼吸链终末氧化酶,是生物体内能量生成的重要步骤。本文利用高分辨率的SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)分离了大鼠肝、肾细胞色素氧化酶的亚单位组分。 相似文献
110.
Platelet factor 4 ( PF4) is a negativehematopoietic factor.It can inhibit the prolifera-tion of endothelial cells and hematopoietic stem/progenitor cells,particularly megakaryoryocyticcells,reversibly[1] ,inhibit DNA synthesis,blockcell cycle progression during S phase and reducethe sensibility of normal hematopoietic stem/pro-genitor cells,but not some cancer or leukemia celllines,to cytotoxic drugs and ionizing radia-tion[2 - 3] ,and it also can cause a population in-crease of the stem cel… 相似文献