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91.
T Rustemeyer S De Ligter B M Von Blomberg P J Frosch R J Scheper 《Clinical and experimental immunology》1999,117(2):209-216
Dendritic cells (DC), generated from adherent peripheral blood mononuclear cells (PBMC) by culturing with granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4, were used to study in vitro sensitization of naive, hapten-specific T cells and to analyse cross-reactivities to related compounds. DC were hapten-derivatized with nickel sulphate (Ni) or 2-hydroxyethyl-methacrylate (HEMA), followed by tumour necrosis factor-alpha (TNF-alpha)-induced maturation, before autologous T cells and a cytokine cocktail of IL-1beta, IL-2 and IL-7 were added. After T cell priming for 7 days, wells were split and challenged for another 7 days with Ni or HEMA, and potentially cross-reactive haptens. Hapten-specificity of in vitro priming was demonstrated by proliferative responses to the haptens used for priming but not to the unrelated haptens. Highest priming efficiencies were obtained when both IL-4 and IL-12 were added to the cytokine supplement. Marked interferon-gamma (IFN-gamma) release (up to 4 ng/ml) was found when IL-12 was included in the cultures, whereas IL-5 release (up to 500 pg/ml) was observed after addition of IL-4 alone, or in combination with IL-12. Nickel-primed T cells showed frequent cross-reactivities with other metals closely positioned in the periodic table, i.e. palladium and copper, whereas HEMA-primed T cells showed distinct cross-reactivities with selected methacrylate congeners. Similar cross-reactivities are known to occur in allergic patients. Thus, in vitro T cell priming provides a promising tool for studying factors regulating cytokine synthesis, and cross-reactivity patterns of hapten-specific T cells. 相似文献
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Stefan Zwerger Lutz Günther Arnulf Pekrun Heico-Rüdiger Krause Jan Rustemeyer 《Oral and maxillofacial surgery》2010,14(4):233-237
Introduction
An orbital embryonal rhabdomyosarcoma (RMS) is a rare malignancy in children, but clinical findings are typical. We detail the case of an 8-year-old female with orbital RMS and discuss the therapeutic options.Case report
Orbital RMS was apparent with painless exophthalmos of the right globe and diplopia. Head MRI showed tumor masses behind and inferior to the globe. Open biopsy led to the histological diagnosis. Metastasis or infiltration of orbital bone was not observed. Chemotherapy was carried out in accordance with the Cooperative Weichteilsarkom Studie (CWS) 2002 protocol. Tumor regression was detected after the first course of chemotherapy; we decided to excise the residual tumor with preservation of the globe. The CWS 2002 protocol was subsequently completed. Radiotherapy was not done. One year after treatment, RMS recurrence was not observed.Conclusion
After interdisciplinary treatment, mutilation was avoided after exenteration of the orbit or radiation treatment to the growing facial skeleton. That was possible due to excision of the residual tumor in a second step, leading to down-staging of the RMS. 相似文献96.
Purpose
We evaluated which factors affect patient satisfaction and if patient expectations were fulfilled after orthognathic surgery.Methods
Questionnaires consisting of 14 questions were given 1 year after bimaxillary osteotomy for class-III correction to subjects. Six questions were answered using an 11-point rating scale based on a visual analog scale (VAS; 0?=?poor; 10?=?excellent). Also included were seven closed-form questions with yes/no answers, as well as one open question for ‘further remarks’. Sagittal and vertical cephalometric parameters were determined on postoperative cephalograms.Results
Seventy-seven patients (37 females, 40 males; mean age, 23.4?±?4.9 (SD) years) responded. The intention to undergo surgery only for aesthetic improvement was noted in 11.9% of patients; only improvement of chewing function in 15.5%; both in 71.4%; and none/don't know in 2.6%. Postoperative satisfaction was rated (in means) with 8.13?±?1.97 on VAS and correlated significantly with the opinions of friends and relatives. Facial aesthetics was rated 5.6?±?1.2 before surgery and 8.1?±?1.5 after surgery (p?=?0.04). Preoperative chewing function was rated 5.65?±?1.8 and 8.03?±?1.51 after surgery (p?=?0.014). TMJ disorders or hypoesthesia had no negative impacts. Cephalometric analyses revealed a significantly lower SNB (75.3°?±?2.7°; p?=?0.033) in patients rating lower than grade 7 for overall satisfaction. For SNA and ArGoMe, no significant differences were observed.Conclusion
The most distinctive factors for patient satisfaction after orthognathic surgery were chewing function and facial aesthetics with respect to the lower face. Function, aesthetics, and even psychological aspects should be considered equally when planning surgery. 相似文献97.
Optimized flow cytometric analysis of endothelial progenitor cells in peripheral blood 总被引:1,自引:0,他引:1
Rustemeyer P Wittkowski W Jurk K Koller A 《Journal of immunoassay & immunochemistry》2006,27(1):77-88
Although flow cytometry is a rapid and convenient way to measure the number of circulating endothelial progenitor cells (EPC), there is no standard technique for preparation and measurement. The aim of this study was to present an optimized preparation method for EPC measurement which should serve as a standard to facilitate the comparison of the results in stem cell investigations by different research groups. We have looked for the preparation method which delivered the best immunostaining with the directly conjugated antibodies against VEGF R2, CD133, CD34, and CD45. In order to test the sensitivity of the method, we determined the number of EPC in the peripheral blood of volunteers by flow cytometry and by cell culture assay. Furthermore, we have evaluated the influence of different durations of conservation on the EPC cell count. The pre-treatment of blood samples with 0.2% formaldehyde for 30 minutes delivers the best immunostaining, and blood samples can be stored overnight at 4 degrees C without loss of counting rate for EPC. We found an excellent correlation (r = 0.98) between the flow cytometric measurement and the cell count of the cell culture method. The presented protocol for the flow cytometric measurement of EPC in the peripheral blood can be used as a diagnostic or prognostic tool; we propose this protocol as the standard for EPC quantification. 相似文献
98.
Niels P. J. de Graaf Albert J. Feilzer Cees J. Kleverlaan Hetty Bontkes Sue Gibbs Thomas Rustemeyer 《Contact dermatitis》2018,79(2):85-90
Background
Titanium is being increasingly used. Although it is considered to be a non‐allergenic material, allergic reactions to it have been reported. Titanium dioxide has been found to be an unreliable patch test material. Few studies to date have profiled titanium allergy, and it therefore remains difficult to distinguish its manifestations.Objectives
To evaluate alternatives for titanium dioxide as a patch test preparation, and to profile titanium reactions and manifestations.Methods
A retrospective chart review was conducted with 458 patients who underwent patch testing with at least 1 of 5 different titanium salts.Results
At least 1 positive result was noted in 5.7% of the patients. The frequency of positive results for the tested salts ranged from 0.9% to 7.9%. Titanium(IV) oxalate hydrate had the highest yield and titanium dioxide the lowest. Erythema, dermatitis and local swelling were the most common objective complaints. In 16 (61.5%) patients, the test result had partial or full clinical relevance.Conclusions
No titanium‐specific risk factors and clinical picture could be identified. Titanium dioxide is not adequately sensitive for identifying titanium allergy. The titanium salts seem to be possible superior patch test preparations, but appear to be unsuitable if used singly. The patient's medical history and clinical picture remain crucial in the diagnostic work‐up. 相似文献99.
Zusammenfassung Es wird eine quantitative papierchromatographische Methode zur Bestimmung von BarbitursÄure-Derivaten im Plasma bei Schlafmittelvergiftungen angegeben. Neben der Möglichkeit einer quantitativen Aussage wird durch die Chromatographie eines Plasmaextraktes parallel zum Urinextrakt die Sicherheit der Identifizierung des eingenommenen PrÄparates erhöht. 相似文献
100.
Rustemeyer J Kranz V Bremerich A 《The British journal of oral & maxillofacial surgery》2007,45(7):556-560
The aim of this review was to examine the range of combat injuries with particular reference to those of the head and neck. We evaluated 10 retrospective studies selected from the period 1982-2005 that covered war injuries from Vietnam, Lebanon, Slovenia, Croatia, Iraq, Somalia, and Afghanistan. We found differences in the causes of injuries. Injuries from fragments were more common during the 90s than during the Vietnam War, where shooting injuries predominated. Injuries to the trunk were reduced in conflicts from 1991 onwards as military personal armour systems including protective vests were used. However, the mortality of wounded soldiers in all conflicts was consistently between 10% and 14%. There was a high incidence of injuries to the head and neck (up to 40%) though they affected only 12% of the body surface area. Though the data from the different military conflicts are not totally comparable, there are trends in the type of injuries and mortality, which may lead to changes in existing systems of medical care. 相似文献