Application of solubilizing properties of the products of oxyethylenated fatty alcohols of the Brij type in relation to simvastatin

Investigation of 3-hydroxy-3-methyl-glutaril-coenzyme A inhibitors (statins) has influenced considerably the process of treatment of dislipidemy; now they belong to the most frequently prescribed hypolipemizing medicines. As it has been proved through randomized clinical examination, it is believed to be related to their complex pharmacological action that statins cause certain clinical effects such as a decrease in the morbidity and mortality rate resulted from vessel diseases referred to artheriosclerotic changes. It has been also proved the statine's beneficial influence during the primary and secondary preventive treatment for ischaemic heart disease, ischaemic stroke in the case of patients with diagnosed changes in the vascular system. Moreover, it has been proved the clinical usability in the preventive treatment for osteoporotic changes in bones [4]. The aim of the preformulary examination is to determine solubilizing properties of oxyethylenated fatty alcohols of the Brij type in the relation to simvastatin. Estimation of pharmaceutical accessibility of simvastatin in model form of drug made it possible to establish the influence of supportive substances on the process of equilibrial solubilization of biologically active substance in the recipe liquid--0.1n HCl.     

The effectiveness of postoperative external beam radiotherapy for incompletely resected non-small cell lung cancer

In a retrospective analysis of 150 incompletely resected NSCLC patients treated with adjuvant external beam radiotherapy 32 (21.3%) survived 3 years with no symptoms of disease. Ipsilateral mediastinal/hilar lymph node involvement and macroscopic incomplete surgery were the prognostic factors that unfavourably influenced survival in Cox's proportional hazards model. Postoperative external beam radiotherapy was the efficient adjuvant treatment method in microscopically incompletely resected NSCLC, predominantly with no nodes involvement, but had no benefit in those with macroscopic incomplete surgery.     

Effect of oxythiamin on growth rate, survival ability and pyruvate decarboxylase activity in Saccharomyces cerevisiae

Oxythiamin is one of the antivitamin derivatives of thiamin which, after phosphorylation, can be bound to the catalytic centre of thiamin-dependent enzymes and inhibit these enzymes. In this work the influence of oxythiamin on the growth rate, survival and the activity of pyruvate decarboxylase of Saccharomyces cerevisiae (s288c) was investigated. Oxythiamin decreased both the growth rate and survival ability of yeast cells. Moreover, in three-day-old cultures on a medium with oxythiamin, an increase of pyruvate decarboxylase activity was observed. This unusual effect may be in response to the earlier inhibition of pyruvate decarboxylase. A high concentration of pyruvate in the cell extracts taken from the medium with oxythiamin was found. This accumulation of pyruvate could provide for enhanced biosynthesis of the pyruvate decarboxylase apoform and an increase of enzyme activity.     

Exuberant cellular reaction of the optic nerves in experimental Creutzfeldt-Jakob disease

We report here on the exuberant glial reaction in the optic nerves affected by prion diseases. Optic nerves from CJD- and GSS-, and scrapie-infected mice and hamsters showed severe pathology. These lesions were qualitatively indistinguishable from each other but were more intense in the Fujisaki model than in the hamsters inoculated with Echigo-1. Exuberant cellular reaction comprised of macrophages containing numerous mitochondria, abundant rough endoplasmic reticulum, and secondary lysosomes filled with digested myelin debris, electron-dense material and occasionally, entire myelin-bound vacuoles were readily observed in both models. Macrophages actively digesting myelin fragments and containing lyre-like bodies and paracrystalline inclusions were frequently noted. Some macrophages extended long filopodia to form labyrinth-like structures, and within a few macrophages, concentric arrays of cisterns and channels sequestrated part of the cytoplasm. An analogous network of narrow cisterns was seen to surround whole segments of the myelinated fibers.     

Food wasting by house mice: variation among individuals, families, and genetic lines

Under ad libitum conditions, laboratory house mice (Mus domesticus) fragment considerable amounts of pelleted food and leave it scattered in their cages. The proportion of food thus wasted (in relation to food eaten) varies remarkably among individuals, from 2% to 40%, but is highly consistent in consecutive trials, even when the mice were moved from 22 to -10 degrees C and food consumption doubled. Food wasting did not differ either between the sexes or between genetic lines that had been selected (10 generations) for high voluntary wheel-running behavior (n=4) and their unselected control lines (n=4). However, it varied significantly among replicate lines within the selection groups and among families within the lines (coefficient of intraclass correlation for full sibs, rhof=0.41 in room temperature trials and rhof=0.34 in cold trials). Moreover, the percent of food wasted was negatively correlated with food consumption in the cold trials (males: r=-.36, females: r=-.20) and with total litter mass at weaning (the litters into which they were born; r=-.24), two traits that may affect Darwinian fitness. We conclude that food wastage should not be ignored without justification in calculations of food consumption. In addition, "table manners" can convey reliable information about family origin of an individual and its quality, and therefore could potentially play a role in establishment of social status.     

Immunologic and genetic aspects of endometriosis physiopathology

This article reviews the pathological mechanism of endometriosis. We concentrate on immunological and genetical factors which play a role in the developing of endometriosis. We present the possible network of interactions between immune cells, hormones and matrix metalloproteases system in the ectopic endometrial tissue.     

Improvement of computer program for cytology screening of the female population in Bialystok

In the article authors present a new edition of computer programme managing active and population cytology screening in the selected group of women in Bialystok. All improvements came out of the slow date base transforming. From the other hand so called "2000 year mistake" was eliminated. In the previous programme single date transfer between only two PC terminals was possible. Additionally way of assignment of particular groups of women to proper out patient rooms districts also by the zip codes were presented. Process of creation of cytology invitations to all women participating in screening examination was accelerate. Increase effectiveness of screening examinations was available as a result of using a new edition of computer programme. Percentage of mistakes and exam expenses were also minimized. Two main cytology screening foundation, its active and population were achieved.     

Diagnostic difficulties in neuroendocrine carcinoma of the larynx

The rare case of the large cell neuroendocrinic carcinoma of larynx was described. 66 years old man was treated by total laryngectomy because of melanoma. Postoperative, immunohistochemical study shoved large cell neuroendocrinic carcinoma. A special emphasis was put on the preoperative misdiagnose, limiting neck dissection. The authors point out, however, the difficulties of the pathologic differentiation caused by similarity of these tumors. The treatment and prognosis are discussed.     

Myeloperoxidase (MPO) as a marker of neutrophil influx into nasal mucosa after recombinant IL-8 challenge

Myeloperoxidase (MPO) has been proposed to mirror the degree of neutrophil activation, however its role as a marker of the participation of neutrophils in allergic inflammation is still unclear as the literature is controversial. The aim of this study was to evaluate the MPO levels and neutrophil influx in nasal lavage after recombinant Il-8 challenge. Eight patients suffering from seasonal allergic rhinitis, hypersensitive to grass pollens, with average age 30.1 +/- 2.67 were challenged both with Il-8 and diluent for Il-8 on a subsequent day, in two phases: before the pollen season (unprimed mucosa) and during the season (primed mucosa). Nasal lavage with saline were collected before, during Il-8 or placebo challenge and 30 minutes, 2 hours and 3 hours after the challenge. The number of neutrophils and MPO levels in the nasal fluid were determined. After the challenge with Il-8 of primed mucosa the number of neutrophils increased from 28250 cells/ml at the baseline to 28778, 251020 and 333660 at 0, 5, 2 and 3 hours respectively. There was a statistically significant difference between cells number after diluent and Il-8 challenge (p < 0.05, Wilcoxon rank-sum test). The number of neutrophils in the nasal lavage of primed mucosa after Il-8 challenge was significantly higher (p < 0.005) at all time points in comparison with the diluent and Il-8 challenges in the unprimed mucosa. There was no difference (p < 0.05) in MPO levels in the nasal lavage between Il-8 (mean 17.43 ng/ml +/- 10.98) and diluent challenge (20.98 ng/ml +/- 11.89) of unprimed mucosa. In the primed mucosa fluid we observed a peak of MPO level at 2 hours time point, however that was not significant as compared to diluent challenge (p = 0.465). We did not find the relationship between MPO levels and the neutrophils number in the lavage (rank Spearman factor, rs = 0.258, p = 0.42). Due to the lack of statistically confirmed relationship between MPO level and the number of neutrophils, MPO seems to be of little value as a marker of neutrophil influx into nasal mucosa.