粉防己碱对DOCA盐性高血压心肌肥厚大鼠心脏血流动力学的影响

粉防己碱可显著降低DOCA盐性高血压心肌肥厚大鼠的血压,左室湿重;在工作心脏研究中发现其改善肥厚心室的收缩(AP,LVSP,+dp/dt_max)舒张(一dp/dt_max,LVEDP,T值)性能,尤以舒张性能改善明显,并恢复泵功能(CO)和冠脉流量(CF);在左室压力容积关系法研究显示有改善肥厚心室左室顺应性和左室僵硬度的作用。提示:Tet有逆转心肌肥厚和改善肥厚心肌舒张收缩功能,左室顺应性和心肌劲度的作用。     

血管紧张素Ⅱ及牛磺酸对体外培养乳鼠肥大心肌细胞心律失常发生的作用

在培养72h的乳鼠心肌细胞,血管紧张素Ⅱ(angiotensinⅡ,AgnⅡ)1,10,100和1000nmol·L^-1时,可浓度依赖性地增加细胞搏动频率;而牛磺酸5,10和20mmol·L^-1不影响心肌细胞搏动频率,但能浓度依赖性地拮抗AngⅡ100nmol·L^-1加快心肌细胞搏动频率的作用。AngⅡ100nmol·L^-1连续作用7d引起的培养乳鼠肥大心肌细胞搏动频率加快,动作电位APA升高,APD₅₀和APD90延长,SCL缩短;哇巴因50nmol·L^-1诱发的心肌细胞搏动节律失常发生率高于常规培养组。牛磺酸20mmol·L^-1可抑制AngⅡ引起的上述改变,并降低哇巴因诱发的心律失常发生率。     

前胡丙素对培养乳鼠心肌细胞自发性收缩及动作电位的影响

用倒置显微镜闭路电视系统及细胞内标准微电极法,记录培养心肌细胞的自发性收缩及动作电位,结果发现:应用Pra-C5min后,心肌细胞收缩频率及细胞边缘运动的速度同时下降。Pra-C10,30和100μmol·L^-1剂量依赖性的方式抑制心肌细胞的收缩速度,分别抑制24%,43%和50%。pra-C(10和30μmol·L^-1)显示了抑制心肌细胞收缩频率的作用,分别抑制13%和19%。Nif3μmol.L^-1分别缩短APD₅₀和APD₉₀14%及17%,但同样浓度的verapatmil则抑制APA27%,缩短APD₅₀8%,分别延长APD90及SCL10%和43%。Pra-C10,30和100μmol·L^-1缩短APD₅₀7%,14%和18%。Pra-C100μmol·L^-1有抑制APA及延长SCL的作用。结果提示,Pra-C对心肌细胞收缩及动作电位的作用可能与其阻滞Ca²⁺通道有关。     

Production and Evaluation of Toxoplasma gondii Recombinant Surface Antigen 1 (SAG1) for Serodiagnosis of Acute and Chronic Toxoplasma Infection in Human Sera

Background

The assays currently available for the detection of specific anti-Toxoplasma antibodies may vary in their abilities to detect serum immunoglobulins, due to the Lack of a purified standardized antigen. The aim of this study was evaluation the recombinant Toxoplasma gondii SAG1 antigen for the serodiagnosis of acute and chronic toxoplasmosis.

Methods

This study describes an ELISA using recombinant SAG1 for detection of IgM and IgG antibodies against Toxoplasma gondii in human sera. Genomic DNA of T. gondii (RH Strain) was isolated and PCR reaction was performed. Recovered DNA was cloned into PTZ57R cloning vector. The recombinant plasmid was detected by restriction analysis. The SAG1 gene was subcloned in the pET- 28a expression vector. Protein production was then induced with 1 mM isopropyl-D – thiogalactopyranoside (IPTG). A total of 204 sera were tested using a commercial IgG and IgM ELISA kit (Trinity, USA) as gold standard prior to testing them with the recombinant antigen.

Results

Tested sera were divided into the following groups:(a) The 74 T. gondii IgG positive (b) 70 T.gondii IgM positive (c) 60 sera who had no serological evidence of toxoplasmosis as negative sera.To determine the specificity of the test, we used other parasitic diseases including echinococusis (N=5), malaria (N=14), leishmaniasis (N=7),fasciolasis (N=4), sterengyloidiasis (N=1). Sensitivity and specificity of the generated recombinant IgG ELISA in comparison with commercial ELISA (Com ELISA) were 93% and 95%, and the sensitivity and specificity of the generated recombinant IgM ELISA were 87% and 95% respectively.

Conclusion

The results acquired here show that this antigen is useful for diagnostic purposes and could be replaced by lysed, whole cell antigens for diagnosis of chronic toxoplasmosis.     

颈椎保健理疗仪的研制

本文介绍了一种适合治疗各种类型颈椎病的颈椎保健理疗仪。它是由颈椎按摩装置、热疗装置和颈椎牵引装置构成。治疗颈椎病时,先利用颈椎按摩、热疗装置对颈部进行揉捏推拿、振动按摩、热疗;然后利用牵引装置对椎间小关节紊乱、椎间隙狭窄（椎间盘病变）等疾病进行牵引治疗。     

Seroepidemiological Study of Human Hydatidosis in Meshkinshahr District,Ardabil Province,Iran

Background

The aim of this study was to conduct a sero-epidemiological survey in Meshkinshahr, Ardabil Province, northwestern Iran to detect the rate of hydatidosis in the city and nearby villages. Literature shows that no such study has been conducted so far.

Methods

Overall, 670 serum samples were collected from 194 males and 476 females from patients referred to different health centers of the region. All patients filled out a questionnaire and an informed consent. Sera were analyzed using indirect-ELISA test. Ten µg /ml antigens (Antigen B derived from hydatid cyst fluid), serum dilutions of 1:500 and conjugate anti-human coombs with 1:10000 dilutions were utilized to perform the test. Data analysis was conducted using SPSS software ver. 11.5.

Results

The seroprevalence of human hydatidosis was 1.79% by ELISA test in the region. This rate for females was 1.68% and males 2.6%, respectively. There was no significant difference as regards all factors studied and the seropositivity. According to job, farmers and ranchmen had the highest rate of infection as 3.17%. The sero-prevalence of infection was 2.6%% in illiterate people which showed the highest rate. As regards residency, urban life showed no significant difference with rural life (1.1% vs. 2.58%). Age group of 69–90 yr old, with 4.62% as prevalence had the highest rate of positivity.

Conclusion

Obtained sero-prevalence of hydatidosis shows more or less a resemblance to other cities of Iran, although due to the specific condition of the city we expected more rate of sero-positivity.     

Superoxide Dismutase (SOD) Enzyme Activity Assay in Fasciola spp. Parasites and Liver Tissue Extract

Background

The purpose of this comparative study was to detect superoxide dismutase (SOD) activities in Fasciola hepatica, F. gigantica parasites, infected and healthy liver tissues in order to determine of species effects and liver infection on SODs activity level.

Methods

Fasciola spp. parasites and sheep liver tissues (healthy and infected liver tissues), 10 samples for each, were collected, homogenized and investigated for protein measurement, protein detection and SOD enzyme activity assay. Protein concentration was measured by Bradford method and SODs band protein was detected on SDS-PAGE. SODs activity was determined by iodonitrotetrazolium chloride, INT, and xanthine substrates. Independent samples t-test was conducted for analysis of SODs activities difference.

Results

Protein concentration means were detected for F. hepatica 1.3 mg/ ml, F. gigantica 2.9 mg/ml, healthy liver tissue 5.5 mg/ml and infected liver tissue 1.6 mg/ml (with similar weight sample mass). Specific enzyme activities in the samples were obtained 0.58, 0.57, 0.51, 1.43 U/mg for F. hepatica, F. gigantica, healthy liver and infected liver respectively. Gel electrophoresis of Fasciola spp. and sheep liver tissue extracts revealed a band protein with MW of 60 kDa. The statistical analysis revealed significant difference between SOD activities of Fasciola species and also between SOD activity of liver tissues (P<.05).

Conclusion

Fasciola species and liver infection are effective causes on SOD enzyme activity level.     

Genetic contributions to pain: a review of findings in humans

Pain represents the major motivating factor for which individuals seek healthcare, and pain responses are characterized by substantial inter‐individual differences. Increasing evidence suggests that genetic factors contribute significantly to individual differences in responses to both clinical and experimental pain. The purpose of this review article was to summarize the current literature regarding genetic contributions to pain, highlighting findings relevant to oral pain where available. A brief discussion of methodologic considerations is followed by a review of findings regarding genetic influences on clinical pain. Next, the literature examining genetic contributions to experimental pain responses is presented, emphasizing genetic associations that have been replicated in multiple cohorts. It is hoped that an enhanced understanding of genetic contributions to pain responses will ultimately improve diagnosis and treatment of clinical pain conditions.     

前胡丙素对肾性高血压左室肥厚大鼠心功能、左室顺应性及心肌胶原含量的影响

观察前胡丙素对肾性高血压左室肥厚（ＬＶＨ）大鼠心功能、左室顺应性及心肌胶原含量的影响。用两肾一夹肾性高血压动物模型，ｉｇ前胡丙素（ＰｒａＣ）９周。结果，与ＬＶＨ组相比，ＰｒａＣ组的ＣＦ／ＨＷＷ及ＣＯ／ＨＷＷ分别增加３１３％和２８９％；ＬＶＳＰ和－ｄｐ／ｄｔｍａｘ负值分别增加１６７％和２７８％；ＬＶＥＤＰ和Ｔ值分别降低３４９％和３６５％；ＰＶ曲线接近正常组水平；左室肌羟脯氨酸降低２３８％。提示ＰｒａＣ可提高ＬＶＨ大鼠心脏收缩及舒张功能，改善心肌顺应性