全文获取类型
收费全文 | 9143篇 |
免费 | 594篇 |
国内免费 | 31篇 |
专业分类
耳鼻咽喉 | 67篇 |
儿科学 | 222篇 |
妇产科学 | 101篇 |
基础医学 | 1337篇 |
口腔科学 | 147篇 |
临床医学 | 612篇 |
内科学 | 2404篇 |
皮肤病学 | 474篇 |
神经病学 | 610篇 |
特种医学 | 220篇 |
外科学 | 1408篇 |
综合类 | 31篇 |
一般理论 | 1篇 |
预防医学 | 313篇 |
眼科学 | 182篇 |
药学 | 663篇 |
中国医学 | 29篇 |
肿瘤学 | 947篇 |
出版年
2023年 | 51篇 |
2022年 | 125篇 |
2021年 | 212篇 |
2020年 | 138篇 |
2019年 | 148篇 |
2018年 | 235篇 |
2017年 | 174篇 |
2016年 | 221篇 |
2015年 | 185篇 |
2014年 | 248篇 |
2013年 | 352篇 |
2012年 | 521篇 |
2011年 | 551篇 |
2010年 | 295篇 |
2009年 | 271篇 |
2008年 | 501篇 |
2007年 | 538篇 |
2006年 | 494篇 |
2005年 | 478篇 |
2004年 | 494篇 |
2003年 | 470篇 |
2002年 | 484篇 |
2001年 | 246篇 |
2000年 | 214篇 |
1999年 | 192篇 |
1998年 | 113篇 |
1997年 | 88篇 |
1996年 | 68篇 |
1995年 | 67篇 |
1994年 | 59篇 |
1993年 | 59篇 |
1992年 | 129篇 |
1991年 | 119篇 |
1990年 | 127篇 |
1989年 | 154篇 |
1988年 | 87篇 |
1987年 | 115篇 |
1986年 | 79篇 |
1985年 | 88篇 |
1984年 | 74篇 |
1983年 | 53篇 |
1982年 | 36篇 |
1981年 | 26篇 |
1979年 | 43篇 |
1978年 | 34篇 |
1977年 | 30篇 |
1972年 | 24篇 |
1971年 | 27篇 |
1969年 | 28篇 |
1967年 | 28篇 |
排序方式: 共有9768条查询结果,搜索用时 15 毫秒
61.
Transforming growth factor beta is protective in host resistance against Listeria monocytogenes infection in mice.
下载免费PDF全文
![点击此处可从《Infection and immunity》网站下载免费的PDF全文](/ch/ext_images/free.gif)
A Nakane M Asano S Sasaki S Nishikawa T Miura M Kohanawa T Minagawa 《Infection and immunity》1996,64(9):3901-3904
The role of transforming growth factor beta (TGF-beta) in host resistance against Listeria monocytogenes infection was studied with mice. The constitutive expression of TGF-beta 1 mRNA was observed in the spleens and livers of mice before and after infection. Injecting the mice with anti-TGF-beta 1 peptide serum resulted in diminished antilisterial resistance, whereas the administration of human platelet-derived TGF-beta 1 enhanced the resistance. Moreover, mice were protected against lethal infection when treated with TGF-beta 1. These results suggest the TGF-beta 1 might be involved in antilisterial resistance. On the other hand, injecting the mice with TGF-beta 1 resulted in a decrease in the titers of endogenous gamma interferon, tumor necrosis factor alpha, and interleukin-6, which are crucial in antilisterial resistance, in sera and in extracts of spleen and liver. Thus, a complicated mechanism might be involved in the role of TGF-beta 1 in host resistance against L. monocytogenes infection. 相似文献
62.
Variation of the conserved neutralizing epitope in influenza B virus victoria group isolates in Japan
下载免费PDF全文
![点击此处可从《Journal of clinical microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
For almost 20 years, the neutralizing-epitope site specific for influenza B virus Victoria group isolates was conserved at the "tip" of the hemagglutinin molecule; however, it was not detected in half of the isolates from the 2002-2003 epidemic in Japan. Amino acid substitutions (D164E or N165K) were observed at the "tip", and the epitope was altered. The viral antigenicities were affected, and human antibodies did not substantially inhibit the hemagglutination in the hemagglutination inhibition tests. It is suspected that such variants will be important in future epidemics. 相似文献
63.
64.
Toshiyasu Shimizu Keiko Sasaki Michio Kato Hideyuki Arimitsu Sadayuki Ochi Naoko Shigemori Eddy Bagus Wasito Takashi Yokochi Takao Tsuji 《Clinical and Vaccine Immunology : CVI》2005,12(1):157-164
We examined the activation of intraperitoneal T cells in BALB/c mice by the Escherichia coli enterotoxin B subunit, which induced a specific Th2 type of T-cell response to intraperitoneally coadministered bovine immunoglobulin G. The numbers of both γδ and αβ T cells increased significantly after intraperitoneal administration of the B subunit in a time-dependent manner; these numbers were not affected by the B-subunit G33D mutant, which is defective in GM1 ganglioside-binding ability. Early after administration a small number of γδ T cells produced either interleukin-4 (IL-4) or gamma interferon, while late after administration primarily IL-10-producing γδ T cells were detected. γδ T cells induced by the B subunit did not express a characteristic V gene over the time course of the study. The induction of γδ T cells did not occur in athymic nu/nu mice but could be induced upon transplantation of fetal AKR thymus-like αβ T cells. γδ T cells in athymic nu/nu mice with a fetal thymic graft predominantly expressed the donor Thy-1.1 antigen but not the host Thy-1.2 antigen. The induction of these T cells, however, could not be restored by coadministration of the B subunit with peritoneal cells from normal mice. These results suggest that the B subunit activates intraperitoneal γδ and αβ T cells in a manner dependent upon its ability to bind to GM1 ganglioside. γδ T cells induced by the B subunit are Th2-type cells derived from the thymus. These γδ T cells may be functionally involved in specific Th2 responses to the B subunit, which possibly acts as an adjuvant through the influence of αβ T cells. 相似文献
65.
66.
Sasayama T Marumoto T Kunitoku N Zhang D Tamaki N Kohmura E Saya H Hirota T 《Genes to cells : devoted to molecular & cellular mechanisms》2005,10(7):627-638
Aurora-A is a centrosomal serine-threonine kinase that regulates mitosis. Over-expression of Aurora-A has been found in a wide range of tumors and has been implicated in oncogenic transformation. However, how Aurora-A over-expression contributes to promotion of carcinogenesis remains elusive. Immunohistochemical analysis of breast tumors revealed that over-expressed Aurora-A is not restricted to the centrosomes but is also found in the cytoplasm. This over-expressed Aurora-A appeared to be phosphorylated on Thr288, which is known to be required for its enzymatic activation. In analogy to Aurora-A's role in oocyte maturation and the early embryonic cell cycle, here we investigated whether ectopically over-expressed Aurora-A can similarly stimulate polyadenylation of mRNA in human somatic cultured cells by interacting with a human ortholog of cytoplasmic polyadenylation element binding protein, h-CPEB. In vitro experiments revealed that Aurora-A binds directly to, and phosphorylates, h-CPEB. We found that polyadenylation of mRNA tails of cyclin B1 and Cdk1 was synergistically stimulated when Aurora-A and h-CPEB were over-expressed, and they were further promoted in the presence of an Aurora-A activator Ajuba. Our results suggest a function of ectopically over-expressed Aurora-A that might be relevant for carcinogenesis. 相似文献
67.
Two infants (4 and 5 months of age) with a febrile episode for 3 and 5 days, respectively, developed skin rashes after the fever subsided and were diagnosed as exanthem subitum. The rash continued for 5 days followed by mild-to-moderate pigmentation. Human herpesvirus-6 and measles virus, which were confirmed by a specific immunofluorescence assay and by electron microscopy, were isolated simultaneously from blood in the acute stage of the disease but not from the convalescent stage. The titer of the herpesvirus-6 in blood was greater than that of measles. Specific serologic assays showed marked seroconversion against human herpesvirus-6 but not to measles virus. The results suggest that dual infection with human herpesvirus-6 and measles virus results in atypical exanthem subitum or modified measles with unique immunologic responses. 相似文献
68.
T Asano Y Yoshikai K Matsumoto G Matuzaki K Nomoto 《Clinical and experimental immunology》1990,81(1):90-96
CD4+ cells from autoimmune-prone C57BL/6 lpr/lpr mice contain two subpopulations, B220-CD4+ and B220+CD4+ cells. Highly purified B220-CD4+ cells from C57BL/6 +/+ and lpr/lpr mice were examined by comparing functional characteristics and expression of cell surface antigens and T cell receptor (TcR)/CD3 complex. Both lpr B220+CD4+ and B220+CD4-CD8- cells, most of which were PgP-1 positive, expressed TcR/CD3 complex on the cell surface at lower level as compared with B220-CD4+ cells of age-matched normal mice. In addition, the B2200-CD4+ cells were heterogeneous on the basis of surface expression of PgP-1 and CD3 antigens. Normal levels of TcR C alpha-, C beta- and V beta 8-specific mRNA were found in the B220-CD4+ cells and B220+CD4+ cells as compared with normal B220-CD4+ cells, while V beta 8-specific mRNA was preferentially expressed only by B220+CD4-CD8- cells. Either B220+CD4+ cells and B220+CD4-CD8- cells failed to respond to anti-CD3 monoclonal antibody (MoAb) as assessed by proliferative responses and production of interleukin-2 (IL-2). However, appreciable levels of reactivity to anti-CD3 MoAb were detected in the B220-CD4+ cells, although the responsiveness of this subset to such stimuli were reduced, compared with those of normal control. These results indicate that the B220-CD4+ cells in lpr mice are phenotypically and functionally distinct from normal B220-CD4+ cells. 相似文献
69.
Shigesaburo Miyakoshi Eiji Kusumi Tomoko Matsumura Akiko Hori Naoko Murashige Tamae Hamaki Koichiro Yuji Naoyuki Uchida Kazuhiro Masuoka Atsushi Wake Yoshinobu Kanda Masahiro Kami Yuji Tanaka Shuichi Taniguchi 《Biology of blood and marrow transplantation》2007,13(7):771-777
Invasive fungal infection (IFI) is a significant complication after allogeneic hematopoietic stem cell transplantation (HSCT); however, we have little information on its clinical features after reduced intensity cord blood transplantation (RICBT) for adults. We reviewed medical records of 128 patients who underwent RICBT at Toranomon Hospital between March 2002 and November 2005. Most of the patients received purine-analogbased preparative regimens. Graft-versus-host disease (GVHD) prophylaxis was a continuous infusion of either tacrolimus 0.03 mg/kg or cyclosporine 3 mg/kg. IFI was diagnosed according to the established EORTC/NIH-MSG criteria. IFI was diagnosed in 14 patients. Thirteen of the 14 had probable invasive pulmonary aspergillosis and the other had fungemia resulting from Trichosporon spp. Median onset of IFI was day 20 (range: 1-82), and no patients developed IFI after day 100. Three-year cumulative incidence of IA was 10.2%. Four of the 13 patients with invasive aspergillosis (IA) developed grade II-IV acute GVHD, and their IA was diagnosed before the onset of acute GVHD. The mortality rate of IFI was 86%. Multivariate analysis revealed that the use of prednisolone >0.2 mg/kg (relative risk 7.97, 95% confidence interval 2.24-28.4, P = .0014) was a significant risk factor for IA. This study suggests that IFI is an important cause of deaths after RICBT, and effective strategies are warranted to prevent IFI. 相似文献
70.
Atsuo Ogura Toshihiko Asano Junichiro Matsuda Minako Koura Masaro Nakagawa Hiroshi Kawaguchi Yutaka Yamaguchi 《Virchows Archiv : an international journal of pathology》1990,417(3):223-228
Summary Glomerular lesions in hereditary nephrotic mice (ICGN strain) were investigated by electron microscopy. The glomeruli of unaffected animals, which appeared normal by light microscopy, had developed an ultrastructural change in the glomerular capillary basement membrane (GCBM). There was a partial thickening of the GCBM with bilaminar splitting of the lamina densa and an electron-dense fibrillar material exhibiting cross-striations. In affected animals, light microscopy revealed a marked thickening of GCBM and an increase of mesangial matrix without cellular proliferaton. By electron microscopy, multilaminar splitting of the lamina densa in the thickened GCBMs and fusion of the epithelial foot processes were observed. In some severely affected animals, immune complex deposition was found in GCBM, but little if any was observed in other animals. In the end, the glomeruli were globally sclerosed. Our findings suggest that initial structural abnormalities in GCBM may play an important role in the onset and development of the disease, though subsequent events such as immune complex deposition would modify the disease. 相似文献