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81.
82.
Sensitization of transformed rat cells to parvovirus MVMp is restricted to specific oncogenes 总被引:9,自引:0,他引:9
N Salomé B van Hille N Duponchel G Meneguzzi F Cuzin J Rommelaere J J Cornelis 《Oncogene》1990,5(1):123-130
The rat cell line FR3T3 was transformed with the retroviral oncogenes v-myc or v-src, with the DNA tumor viruses SV40 or bovine papilloma virus strain 1 (BPV-1) or with the 69% transforming region of BPV-1. The transformants were compared with the uncloned parental line for their susceptibility to the lytic effect and to the replication of MVMp, an autonomous parvovirus. Expression of v-myc and v-src proteins and of SV40 large T antigen correlated with a greater cell susceptibility to MVMp-induced killing. Thus, the expression of both cytoplasmic and nuclear oncogene products may sensitize rat fibroblasts to MVMp. In contrast, cell lines transformed by BPV-1, including highly tumorigenic and tumor-derived clones, were on the average as resistant as the parental cell line to MVMp infection. A similar resistance to MVMp-induced killing was displayed by BPV-1-transformed NIH3T3 cells. However, supertransformation of one of the BPV-1-transformants by the human EJ-Harvey ras-1 oncogene, known to sensitize FR3T3 and NIH3T3 cells, correlated with an increase in susceptibility to MVMp. Therefore, the failure of BPV-1 transformation to sensitize murine cells to parvoviral attack may be ascribed to the tumor virus rather than to the cells undergoing transformation. Hence, cell sensitization to MVMp appears to be oncogene-specific and cannot be taken as an absolute correlative with neoplastic transformation. 相似文献
83.
Herlitz junctional epidermolysis bullosa keratinocytes display heterogeneous defects of nicein/kalinin gene expression. 总被引:9,自引:3,他引:6
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C Baudoin C Miquel C Blanchet-Bardon C Gambini G Meneguzzi J P Ortonne 《The Journal of clinical investigation》1994,93(2):862-869
Previous studies have correlated the Herlitz junctional epidermolysis bullosa (H-JEB) to an altered expression of the basement membrane component nicein/kalinin. This heterotrimeric glycoprotein appears to be present in H-JEB tissues in an abnormal form, because a number of antibodies specific to the protein either do not react with or weakly stain the epidermal basement membranes of most of the patients. With cDNA probes encoding each subunit of nicein and polyclonal antibodies raised against bacterial fusion polypeptides corresponding to the individual chains of the protein, we have molecularly analyzed the expression of nicein in H-JEB tissues and cultured keratinocytes. By immunohistochemistry, Northern blot, and protein analysis, we show a defective synthesis of one of the nicein subunits in six cases of H-JEB from five different consanguineous families. In two patients, the disease correlates with an impaired synthesis of the nicein B2 (nic B2) chain, in three others with that of the B1 (nic B1) chain, and in a sixth patient with that of the heavy A (nic A) chain. In this report, we thus demonstrate that H-JEB is a genetically heterogeneous disease and we provide strong evidence that the genes of nicein are the candidates for this genodermatosis. 相似文献
84.
The transformed phenotype in culture and tumorigenicity of Fischer rat fibroblast cells (FR3T3) transformed with bovine papilloma virus type 1 总被引:4,自引:0,他引:4
M Grisoni G Meneguzzi O de Lapeyrière B Binétruy M Rassoulzadegan F Cuzin 《Virology》1984,135(2):406-416
Unlike cell lines transformed with Polyomaviruses, transformants derived by focus formation or colony formation in agarose medium after transfer into rat fibroblast cells (FR3T3 line) of Bovine Papilloma Type 1 (BPV1) DNA were consistently observed to grow poorly in suspension and to remain highly serum dependent for growth in culture. These cells did not produce detectable amounts of plasminogen activator, and kept the flat morphology and organized cytoskeleton characteristic of the normal fibroblast. However, they induced in syngeneic animals the development of tumors with a greater invasive potential than tumors induced by Polyomaviruses. By contrast with the original transformants, cells recovered from the tumors grew efficiently in suspension and produced high levels of plasminogen activator. They still had, however, extended cytoskeletal structures and remained completely dependent on high serum concentrations for growth in culture. The stepwise transformation process induced by BPV1 thus appears strikingly different from that previously observed with polyoma and SV40 viruses. The observed changes in transformation phenotype between transformed line and tumor cells do not correlate with any important modification of the number of autonomous copies of the viral genome, nor with any rearrangement of viral sequences detectable at the level of the blot analysis. 相似文献
85.
86.
Castiglia D Posteraro P Spirito F Pinola M Angelo C Puddu P Meneguzzi G Zambruno G 《The Journal of investigative dermatology》2001,117(3):731-739
Laminin-5 is the major adhesion ligand of epithelial cells. Mutations in the three genes (LAMA3, LAMB3, LAMC2) encoding the laminin-5 chains cause junctional epidermolysis bullosa, a clinically and genetically heterogeneous blistering skin disease. Here, we describe a non-Herlitz junctional epidermolysis bullosa patient, compound heterozygote for two novel mutations affecting the LAMC2 gene. The mutation in the paternal allele is a de novo splice site mutation (522-1G-->A) that results in in-frame skipping of exon 4 and synthesis of a mutated gamma2 polypeptide (gamma2Delta4) carrying a 33 amino acid deletion within the N-terminal domain V. The maternal mutation is a one base pair insertion (3511insA) in the 3' terminal exon of LAMC2 resulting in a frameshift and a premature termination codon. Mutation 3511insA is predicted to lead to the synthesis of a gamma2 polypeptide (gamma2t) disrupted in its alpha-helical C-terminal structure and truncated of the last 25 amino acids. Keratinocytes isolated from the patient's skin showed a markedly decreased level of gamma2 chain mRNA and secreted scant amounts of laminin-5, which undergoes physiologic proteolytic processing. To investigate the biologic function of the laminin-5 molecules synthesized by the patient, mutant gamma2 cDNAs were transiently expressed in gamma2-null keratinocytes. Transfection of the gamma2Delta4 cDNA resulted in restoration of laminin-5 deposition onto the culture substrate, which demonstrates that the gamma2 polypeptides carrying a deletion in domain V, upstream of the gamma2 proteolytic cleavage site, are assembled into native laminin-5 that is secreted and extracellularly processed. In contrast, transfection of a mutant cDNA expressing the gamma2t chain failed to restore laminin-5 immunoreactivity, which indicates that integrity of the gamma2 C-terminal amino acid sequences is required for laminin-5 assembly. These results correlate for the first time a functional alteration in a laminin-5 domain with a mild junctional epidermolysis bullosa phenotype. 相似文献
87.