Cardiovascular Implantable Electronic Device Infection in Patients with Staphylococcus aureus Bacteremia

Background: Staphylococcus aureus bacteremia (SAB) in patients with cardiovascular implantable electronic devices (CIED), including permanent pacemakers (PPMs) and implantable cardioverter‐defibrillators (ICD), can be the sole manifestation of device infection. Methods: To assess clinical factors associated with CIED infection, we retrospectively reviewed all patients with both CIED and SAB seen at Mayo Clinic Rochester between 2001 through 2006. CIED infection was defined using microbiological and clinical criteria. Results: Of the 62 patients with SAB and a CIED, 22 patients (35.5%) had CIED infection. The generator pocket was identified as the source of bacteremia in seven (11%) patients. The majority of CIED infections were device‐related infective endocarditis (12 of 22, 55%). Thirty percent of patients presenting with SAB greater than 1 year after device implantation had CIED infection; all but one had CIED‐related infective endocarditis. Sixty percent of ICD patients (12 of 20) with SAB had CIED infection, compared with 24% of PPM patients (10 of 42, P = 0.01). On univariate analysis factors associated with CIED‐related infective endocarditis included device type [odds ratio (OR) for ICD 13.3, 95% confidence interval [CI] 2.1, 84.9) and presence of a prosthetic heart valve (OR 6.8 95% CI 1.1, 43.4). Conclusions: CIED infection is common in patients with SAB. The presence of an ICD and prosthetic heart valve were associated with CIED‐related infective endocarditis. Subsequent work should focus on prospectively characterizing the subset of patients with CIED infection who present with SAB as the sole manifestation of their device infection. (PACE 2010; 407–413)     

Hydrolysis and Stability of Acetylsalicylic Acid in Stearylamine-containing Liposomes

Abstract— The hydrolysis and the stabilization of acetylsalicylic acid (ASA) in liposomes at 30°C were studied. The liposomes consisted of dimyristoylphosphatidylcholine (DMPC) and stearylamine. At pH 4·0 and 8·0, the pseudo-first-order rate constants (k_obs) in DMPC: stearylamine (2:1 mole ratio) liposomes were approximately 60% of the values in control solutions (k_B) if ASA was incorporated via the organic phase. In contrast, when ASA was added via the aqueous phase, k_obs = k_B at pH 4·0 but k_obs < kB at pH 8·0 and k_obs increased with the fraction of stearylamine in the liposomes. However, when ASA was added via the organic phase, reactions occurred which resulted in the loss of ASA as a function of the time period between phase admixture and the point of film hydration. A product of the reactions was determined by IR and TLC to be N-stearylacetamide. Both the initial loss of ASA and the increase in stability decreased as the DMPC: stearylamine mole ratio increased. A mechanism of aminolysis occurring in the organic solvent and at liposome surfaces between ASA and stearylamine or DMPC at pH 8·0 has been suggested. It is concluded that the orientation of ASA and the ordered structural environment of the bilayers minimizes the aminolytic and hydrolytic reactions.     

Development of hepatocellular carcinoma in a man with auto-immune chronic active hepatitis

A 57 year old man with auto-immune chronic active hepatitis, regularly treated with immunosuppressive therapy, had hepatocellular carcinoma (HCC) 10 years after diagnosis of the hepatitis. Assays of the hepatitis C virus antibodies against capsid and non-structural proteins revealed seronegativity in serial serum samples of this patient stored in the previous 10 years during follow up. The seronegative hepatitis C antibodies excluded hepatitis C virus as the cause of the HCC. The occurrence of HCC in this case suggests the necessity of surveillance for early detection of liver cancer in patients with auto-immune chronic active hepatitis undergoing long-term immunosuppressive therapy.     

荧光猝灭法测定盐酸胺碘酮含量

目的:建立荧光猝灭法测定盐酸胺碘酮片剂含量的新方法。方法:试验多种荧光试剂加入系列浓度的胺碘酮溶液,测定其荧光光谱的变化。结果:选用芘丁酸(PBA)为荧光试剂,浓度为2.0mg·L~(-1),λ_(ex)/λ_(em)=349nm/376.3nm,在2.0～20mg·L~(-1)胺碘酮浓度范围与荧光猝灭值(F_0/F)成良好的线性关系(r=0.9962),据此提出测定片剂中胺碘酮含量的荧光猝灭法。结论:荧光猝灭法简便灵敏、选择性高,可用于药片中盐酸胺碘酮含量的测定。     

金樱根中1个新的乌苏烷型三萜皂苷

目的 研究蔷薇科植物金樱子Rosa laevigata根的化学成分.方法 采用硅胶、Sephadex LH-20、ODS柱色谱、重结晶及半制备液相分离和纯化,根据理化性质和波谱数据鉴定化合物的结构,并运用MTT法对所分离化合物对人宫颈癌HeLa、人胃腺癌BGC823、人结肠癌HCT-116及人肝癌HepG-2细胞的细胞...     

A Novel Splice Site Mutation in the EDAR Gene Underlies Autosomal Recessive Hypohidrotic Ectodermal Dysplasia in a Pakistani Family

Abstract:  Hypohidrotic ectodermal dysplasia is a rare congenital disorder that results in abnormalities in the structures of ectodermal origin: hair, teeth, and eccrine sweat glands. DNA sequence analysis of EDAR gene in a Pakistani family, demonstrating autosomal recessive form of hypohidrotic ectodermal dysplasia, identified a novel homozygous mutation affecting splice donor site of exon 5 [IVS5+1G ≥ C] of the gene.     

阿里红中总三萜酸的提取工艺优选及含量测定

目的:优化阿里红中总三萜酸的提取工艺,并建立其含量测定方法。方法:以齐墩果酸为对照品,采用分光光度法测定三萜酸含量;采用单因素试验考察提取溶剂种类及浓度、料液比、温度、提取时间及提取次数对总三萜酸得率的影响,在此基础上,采用L9(34)正交试验优选阿里红中总三萜酸提取工艺。结果:最佳提取工艺为30倍量90%乙醇于60℃超声提取3次,每次30 min。在此条件下,阿里红中总三萜酸质量分数35.1%。各因素影响次序为提取温度>液料比>提取时间>乙醇体积分数。结论:阿里红中三萜酸含量很高,该优选工艺稳定可行,可推广于大生产。