Analysis of Electromagnetic Exposure in Wireless Power Transfer System for Endoscopic Micro-robot

A wireless power transfer system for endoscopic micro-robot operating at 36 kHz is presented in this paper. The issue of patient＇ s health and safety regarding exposure to the electromagnetic field is addressed. The specific absorption rate and current density can be used to investigate the electromagnetic influences on the biological tissues surrounded by the wireless power launching coil. In view of this purpose, the limited close-ound solenoid electromagnetic model is built, the relationship between the electric intensity and the specific absorption rate and current density is deduced, and the simulation experiments are done. Experimental results show that the values of SAR and current density related to different tissue catalogs are all very small and do not exceed their own limits respectively when the resonance frequency of operation is 36 kHz.     

pp~(60c-src(+))在神经生长端的表达及其意义

目的　探索 pp60c-src( + ) 在神经生长端的表达特征及其生理意义。方法　用免疫细胞化学方法检测 pp60c-src( + ) 在初代培养鸡胚脊神经节细胞内的分布特征。结果　pp60c -src( + ) 的免疫活性分布在神经细胞的细胞膜下、核周体、神经突起 ;在生长端体部和丝状伪足 ,pp60c -src( + ) 的免疫活性较强 ,少数免疫活性较弱。在伸长的突起上有时可见 pp60c-src( + ) 免疫活性分布在串珠样膨体上。结论　pp60c-src( + ) 参与生长端的运动和生长 ;在生长端分化、神经生长过程中 ,pp60c-src( + ) 的调控作用具有时空特异性     

Allergologic exploration of germins and germin-like proteins,a new class of plant allergens

BACKGROUND: Germins and the related germin-like proteins (GLPs) are glycoproteins expressed in many plants in response to biotic and abiotic stress. To test the potential impact of germins and GLPs, recombinant germin from Triticum aestivum (tGermin) and GLPs from Arabidopsis thaliana (tGLP), both produced in transformed tobacco plants, were used. METHODS: Sera from 82 patients with type I allergy to birch, grass or mugwort pollen and/or wheat were tested in immunoblot for IgE binding to tGermin and tGLP, and the IgE reactivity after chemical and enzymatic deglycosylation was analysed. The biological activity of tGermin and tGLP was determined in a histamine release assay and in skin prick testing (SPT). RESULTS: In an immunoblotting assay, 24 out of 82 tested sera (29.26%) from allergic patients showed IgE-binding to tGermin, and 18 of these sera (21.95%) displayed also IgE-binding to tGLP. The deglycosylation experiments indicated that glycan moieties contribute significantly to the IgE-binding of tGermin and tGLP. Both tGermins and tGLP induced specifically histamine release in an in vitro assay as well as in SPT. CONCLUSION: Our in vitro and in vivo findings demonstrate that germin and GLPs are capable to bind IgE most likely via carbohydrate determinants, and represent allergenic molecules.     

低营养及低氧状态下NIH3T3细胞增殖及细胞周期的变化及对bFGF的影响

目的： 体外模拟慢性创面缺氧、低营养环境，观察成纤维细胞在该状态下增殖及细胞周期的变化及对外源性生长因子（bFGF）的反应,探讨低氧、低营养条件下成纤维细胞的病理生理变化。方法: 单纯缺氧环境采用厌氧培养箱，通入混合气，氧分压（PO₂）分为27 mmHg和44 mmHg 2个水平；低营养环境则控制培养液新生牛血清（NCS）浓度。用MTT法检测细胞活性以及其对外源性生长因子的反应，用流式细胞仪检测细胞周期。结果: PO₂ 44 mmHg时细胞增殖速度较同期对照组无明显差异；PO₂ 27 mmHg时，细胞增殖速度较同期对照组明显减慢（P<0.01），细胞被阻滞于G₀期，S期细胞比例明显减少，bFGF未显示促增殖作用。NCS浓度为0.5%的低营养状态下细胞增殖速度较同期对照组明显减慢（P<0.01），细胞被阻滞于G₀-G₁期（P<0.01）；bFGF能明显改善低营养状态下的增殖减慢（P<0.01），使G₂-M期细胞比例增加（P<0.05）。结论: 27 mmHg PO₂或NCS浓度为0.5%的低营养环境使细胞阻滞于G₀-G₁期，影响成纤维细胞增殖；bFGF可以改善低营养条件下细胞增殖减慢的状态，但对极度缺氧条件下的成纤维细胞增殖障碍无明显作用。     

Comparison of contact and spatial repellency of catnip oil and N,N-diethyl-3-methylbenzamide (deet) against mosquitoes

Nepetalactone, the primary component of catnip oil, was compared with the repellent N,N-diethyl-3-methylbenzamide (deet) for its ability to affect the host-seeking ability of Aedes aegypti (L.). A triple cage olfactometer was used to bioassay each substance and to assess its attraction inhibition (spatial repellent) attributes when combined with the following attractants: carbon dioxide, acetone, a blend of L-lactic acid and acetone, and human odors. Repellent tests were conducted with each substance against female Ae. aegypti, Anopheles albimanus Weidemann, and Anopheles quadrimaculatus Say. Catnip oil and deet were both weakly attractive to Ae. aegypti, catnip oil was the better spatial repellent, whereas deet was a more effective contact repellent in tests with all three species of mosquitoes.     

模拟股骨置入人工关节扭转与应力松弛蠕变的实验研究

研究了在相同扭矩作用下,正常组股骨和以生物学和骨水泥固定置入人工关节组股骨标本扭转角度,还对正常股骨、生物学固定股骨和骨水泥固定股骨进行应力松弛、蠕变实验,为临床提供生物力学参数。以电子万能试验机对正常对照组、股骨置入人工关节骨水泥固定组、股骨置入人工关节生物学固定组标本进行扭转和粘弹性实验研究。得出了各组标本在相同扭矩下的扭转角度及悬臂弯曲状态下,正常组和置入人工关节组标本应力松弛、蠕变数据和曲线。对实验数据进行归一化处理,得出了正常组和置入人工关节组标本的归一化应力松弛函数,归一化蠕变函数及曲线。表明:骨水泥固定组扭转角比生物学固定组小,说明骨水泥固定有较好的稳定性,其应力松弛、蠕变量丢失小。骨水泥固定组较生物学固定组3600s应力松弛、蠕变量大。     

人缺血脑组织中IP-10和IFN-γ的表达

目的：探讨趋化因子IP-10和细胞因子IFN-γ是否参与人缺血脑损伤过程。方法：将21例脑梗死死亡病例按发病持续时间分为〈7天、7～14天和15～21天3组，以非缺血侧半球做对照，用HE染色法观察炎性细胞浸润情况；通过免疫组织化学方法检测缺血半球脑组织与非缺血半球脑组织中趋化因子IP-10和细胞因子IFN-γ的表达。结果：在〈7天组和7～14天组中，缺血脑组织可见大量炎性细胞浸润。在〈7天组、7～14天组和15～2l天组中，趋化因子IP-10在缺血半球脑组织中的表达高于非缺血半球（分别是1．74倍增高，P〈0．01；1．41倍增高，P〈0．05和1．52倍增高，P〈0．01）。在对细胞因子IFN-γ的检测中发现〈7天和7～14天组中，IFN-γ在缺血半球脑组织中的表达高于非缺血半球（分别是1．65倍增高，P〈0．05和1．32倍增高，P〈0．05）；在15～21天组中，IFN-γ在缺血半球与非缺血半球中的表达没有显著差异（P〉0．05）。结论：在人缺血脑组织中观察到IP-10和IFN-γ的表达，提示IP-10和IFN-γ参与了炎症反应对脑组织的损伤过程。同时也提示IP-10可能参与后期对损伤脑组织的修复。     

抗SARS-CoV抗原的人源Fab段噬菌体抗体库的构建

目的 :利用抗SARS冠状病毒IgG抗体阳性的SARS康复患者外周血淋巴细胞 ,构建人源Fab段抗体文库。方法 :制备外周血淋巴细胞总RNA ,逆转录成cDNA。以其为模板 ,利用针对家族特异性Ig基因的引物扩增重链Fd段和轻链基因 ,并重组到噬菌粒载体pComb3中 ,将重组噬菌粒载体电转化大肠杆菌XL 1Blue,酶切鉴定抗体库的重组率 ,并测定噬菌体抗体库的库容量。结果 :构建了源于SARS康复患者血清中抗Fab段的抗体文库 ,轻链、重链Fd段基因的重组率分别为91%和 75 % ,库容量为 7.2 3× 10 7。结论 :成功地构建了抗SARS CoV抗原的人源Fab段噬菌体抗体库