全文获取类型
收费全文 | 6522篇 |
免费 | 485篇 |
国内免费 | 19篇 |
专业分类
耳鼻咽喉 | 53篇 |
儿科学 | 208篇 |
妇产科学 | 143篇 |
基础医学 | 980篇 |
口腔科学 | 98篇 |
临床医学 | 708篇 |
内科学 | 1258篇 |
皮肤病学 | 164篇 |
神经病学 | 703篇 |
特种医学 | 161篇 |
外科学 | 631篇 |
综合类 | 52篇 |
一般理论 | 11篇 |
预防医学 | 685篇 |
眼科学 | 114篇 |
药学 | 416篇 |
中国医学 | 9篇 |
肿瘤学 | 632篇 |
出版年
2024年 | 10篇 |
2023年 | 99篇 |
2022年 | 176篇 |
2021年 | 302篇 |
2020年 | 209篇 |
2019年 | 269篇 |
2018年 | 263篇 |
2017年 | 222篇 |
2016年 | 240篇 |
2015年 | 231篇 |
2014年 | 310篇 |
2013年 | 391篇 |
2012年 | 591篇 |
2011年 | 547篇 |
2010年 | 273篇 |
2009年 | 279篇 |
2008年 | 418篇 |
2007年 | 406篇 |
2006年 | 361篇 |
2005年 | 335篇 |
2004年 | 280篇 |
2003年 | 246篇 |
2002年 | 225篇 |
2001年 | 29篇 |
2000年 | 17篇 |
1999年 | 18篇 |
1998年 | 34篇 |
1997年 | 18篇 |
1996年 | 19篇 |
1995年 | 15篇 |
1994年 | 17篇 |
1993年 | 19篇 |
1992年 | 10篇 |
1991年 | 6篇 |
1990年 | 6篇 |
1988年 | 10篇 |
1987年 | 4篇 |
1986年 | 7篇 |
1985年 | 9篇 |
1984年 | 5篇 |
1983年 | 6篇 |
1982年 | 9篇 |
1981年 | 11篇 |
1980年 | 9篇 |
1979年 | 4篇 |
1977年 | 3篇 |
1969年 | 6篇 |
1958年 | 3篇 |
1954年 | 3篇 |
1926年 | 3篇 |
排序方式: 共有7026条查询结果,搜索用时 15 毫秒
101.
Cotransport of reduced glutathione with bile salts by MRP4 (ABCC4) localized to the basolateral hepatocyte membrane 总被引:16,自引:0,他引:16
Rius M Nies AT Hummel-Eisenbeiss J Jedlitschky G Keppler D 《Hepatology (Baltimore, Md.)》2003,38(2):374-384
The liver is the major source of reduced glutathione (GSH) in blood plasma. The transport protein mediating the efflux of GSH across the basolateral membrane of human hepatocytes has not been identified so far. In this study we have localized the multidrug resistance protein 4 (MRP4; ABCC4) to the basolateral membrane of human, rat, and mouse hepatocytes and human hepatoma HepG2 cells. Recombinant human MRP4, expressed in V79 hamster fibroblasts and studied in membrane vesicles, mediated ATP-dependent cotransport of GSH or S-methyl-glutathione together with cholyltaurine, cholylglycine, or cholate. Several monoanionic bile salts and the quinoline derivative MK571 were potent inhibitors of this unidirectional transport. The K(m) values were 2.7 mmol/L for GSH and 1.2 mmol/L for the nonreducing S-methyl-glutathione in the presence of 5 micromol/L cholyltaurine, and 3.8 micromol/L for cholyltaurine in the presence of 5 mmol/L S-methyl-glutathione. Transport of bile salts by MRP4 was negligible in the absence of ATP or without S-methyl-glutathione. These findings identify a novel pathway for the efflux of GSH across the basolateral hepatocyte membrane into blood where it may serve as an antioxidant and as a source of cysteine for other organs. Moreover, MRP4-mediated bile salt transport across the basolateral membrane may function as an overflow pathway during impaired bile salt secretion across the canalicular membrane into bile. In conclusion, MRP4 can mediate the efflux of GSH from hepatocytes into blood by cotransport with monoanionic bile salts. 相似文献
102.
Rittersma SZ van der Wal AC Koch KT Piek JJ Henriques JP Mulder KJ Ploegmakers JP Meesterman M de Winter RJ 《Circulation》2005,111(9):1160-1165
103.
Rissanen TT Korpisalo P Markkanen JE Liimatainen T Ordén MR Kholová I de Goede A Heikura T Gröhn OH Ylä-Herttuala S 《Circulation》2005,112(25):3937-3946
104.
Peak oxygen intake and cardiac mortality in women referred for cardiac rehabilitation 总被引:3,自引:0,他引:3
Kavanagh T Mertens DJ Hamm LF Beyene J Kennedy J Corey P Shephard RJ 《Journal of the American College of Cardiology》2003,42(12):2139-2143
OBJECTIVES: This study investigated the prognostic importance of measured peak oxygen intake (VO(2peak)) in women with known coronary heart disease referred for outpatient cardiac rehabilitation. BACKGROUND: Exercise capacity is a powerful predictor of prognosis in men with known or suspected coronary disease. Similar findings are described in women, but fewer studies have utilized measured VO(2peak), the most accurate measure of exercise capacity. METHODS: A single-center design took data from 2,380 women, age 59.7 +/- 9.5 years (1,052 myocardial infarctions, 620 coronary bypass procedures, and 708 with proven ischemic heart disease), who underwent cardiorespiratory exercise testing. They were followed for an average of 6.1 +/- 5 years (median 4.5 years, range 0.4 to 25 years) until cardiac and all-cause death. RESULTS: We recorded 95 cardiac deaths and 209 all-cause deaths. Measured VO(2peak) was an independent predictor of risk, values > or =13 ml/kg/min (3.7 multiples of resting metabolic rate) conferring a 50% reduction in cardiac mortality (hazard ratio [HR] 0.5, p = 0.001). Considered as a continuous variable, a 1 ml/kg/min advantage in initial VO(2peak) was associated with a 10% lower cardiac mortality. Adverse predictors were diabetes (HR 2.73, p = 0.0005) and antiarrhythmic therapy (HR 3.93, p = 0.0001). CONCLUSIONS: As in men, measured VO(2peak) is a strong independent predictor of cardiac mortality in women referred for cardiac rehabilitation. 相似文献
105.
Marko Lempinen Leena Halme Kirsti Numminen Johanna Arola Arno Nordin Heikki Mäkisalo 《Journal of hepato-biliary-pancreatic sciences》2005,12(5):409-414
Hepatobiliary cystadenomas and cystadenocarcinomas are rare tumors. Differentiating between these tumors and benign hepatic cysts may be difficult. Because of their rarity, diagnosis is often delayed and may result in inaccurate treatment, resulting in unnecessary morbidity and mortality. The purpose of this report is to draw attention to these entities and their complications. We report on two cases with spontaneous rupture of hepatobiliary cystadenoma and cystadenocarcinoma cysts, initially treated as simple hepatic cysts by aspiration, or by aspiration combined with sclerotherapy. The spontaneous rupture of the cysts appeared years after the initial treatment of the cysts, leading in one case to a prolonged stay in an intensive care unit. In both cases, a formal liver resection was carried out and microscopic investigations revealed a mucinous cystadenocarcinoma and cystadenoma. In conclusion, although hepatobiliary cystadenomas and cystadenocarcinomas are rare findings, they should not be forgotten in the diagnostic workshop when examining patients with hepatic cysts. If hepatobiliary cystadenomas and cystadenocarcinomas cannot be excluded following radiological imaging, surgery is recommended. 相似文献
106.
107.
Kwun Lam Anneli Peolsson Emiliano Soldini Hkan Lfgren Johanna Wibault sa Dedering Birgitta
berg Peter Zsigmond Marco Barbero Deborah Falla 《Medicine》2021,100(8)
Pain as a result of cervical radiculopathy (CR) can be widespread, nondermatomal and individually specific, but the association between pain extent and other clinical features has never been explored. The objective of this study is to investigate whether pain extent relates to clinical variables including pain intensity in addition to health indicators including disability, general health, depression, somatic anxiety, coping strategies or self-efficacy.An observational cohort study was conducted. Participants were recruited from 4 hospital spinal centres in Sweden. Pain extent was quantified from the pain drawings of 190 individuals with cervical disc disease, verified with magnetic resonance imaging (MRI) and compatible with clinical findings (examined by a neurosurgeon), that show cervical nerve root compression. Pain extent was evaluated in relation to neck pain, arm pain, and headache intensity. Multiple linear regression analysis were then used to verify whether pain extent was associated with other health indicators including disability, health-related quality of life, depression, somatic anxiety, coping strategies and self-efficacy.Pain extent was directly related to neck, arm and headache pain intensity (all P < .01). Multiple linear regression revealed that pain extent was significantly associated only to the level of perceived disability (P < .01).Increased pain extent in people with CR is associated with higher headache, neck and arm pain intensity, and disability but not measures of general health, depression, somatic anxiety, coping strategies or self-efficacy. 相似文献
108.
Huijin Dong Johanna R. Mora Catherine Brockus Shannon D. Chilewski Robert Dodge Colin Merrifield W. Matthew Dickerson Binodh DeSilva 《The AAPS journal》2015,17(6):1511-1516
Immunogenicity testing for PEGylated biotherapeutics should include methods to detect both anti-protein and anti-PEG antibodies (anti-PEG). Although some methods have been published for the detection of anti-PEG antibodies, the information is incomplete and, in some cases, reagents used (such as Tween-20) are known to interfere with detection. This rapid communication describes the use of BioScale’s Acoustic Membrane MicroParticle (AMMP®) technology using the ViBE® Workstation to measure anti-PEG antibodies in human serum samples. Briefly, a sample spiked with monoclonal human IgG anti-PEG antibody is diluted in buffer and incubated with paramagnetic beads coated with linear chain mPEG to capture anti-PEG antibodies. The complex is then captured on an acoustic membrane coated with Protein A. The change in mass on the membrane caused by the binding of the complex to the membrane results in a signal proportional to the mass of anti-PEG antibodies. The data indicate that an assay with a sensitivity of less than 1000 ng/mL for IgG is achievable. This level of sensitivity is better than current published reports on IgG anti-PEG antibody detection.KEY WORDS: acoustic membrane microparticle technology, anti-peg antibodies, emerging technology, immunogenicity assays, pegylated biotherapeutics 相似文献
109.
110.
David A. Talan Sukhjit S. Takhar Anusha Krishnadasan William R. Mower Daniel J. Pallin Manish Garg Jon Femling Richard E. Rothman Johanna C. Moore Alan E. Jones Frank Lovecchio Jonathan Jui Mark T. Steele Amy M. Stubbs William K. Chiang Gregory J. Moran 《Annals of emergency medicine》2021,77(1):32-43