4种便携式血糖仪与生化分析仪的一致性和抗干扰能力的研究

目的 通过对不同便携式血糖仪与贝克曼库尔特A U 5800全自动生化分析仪(A U 5800)检测血糖结果的一致性比对和抗干扰能力的研究,为临床选择适合的血糖仪提供参考.方法 共选取4种不同检测原理的2台进口(A和B)和2台国产(C和D)血糖仪,与A U 5800同时检测不同浓度的血糖并进行比较,分析其一致性.分别检测...     

三维有限元分析在牙周夹板研究中的应用

牙周炎是口腔常见病、多发病,是牙齿松动的主要原因之一.牙周夹板一直以来是松牙固定的有效手段,它可以将多个牙连成一个整体,形成一个“多根巨牙”,共同承担咬合力,有效地分散牙周膜及牙槽骨的应力,为牙周组织的愈合创造有利条件.三维有限元分析法是研究复杂模型应力分布的生物力学方法,近年来广泛应用于牙周夹板的研究中,该方法可以逼...     

B-cell growth factor receptor expression and B-cell growth factor response of leukemic B cell precursors and B lineage lymphoid progenitor cells

The purpose of this study was to analyze the expression of B cell growth factor (BCGF) receptors and to elucidate the biologic effects of biochemically purified natural BCGF at the B cell precursor stage of human B lineage lymphoid differentiation. The specific binding of radioiodinated high-mol-wt BCGF (125I-HMW-BCGF) and low-molecular-wt BCGF (125I-LMW-BCGF) to fresh marrow blasts from B cell precursor acute lymphoblastic leukemia (ALL) patients was initially investigated. The estimated number of radioiodinated BCGF molecules bound per blast ranged from undetectable to 24.3 X 10(3) for HMW-BCGF, and from 11.5 X 10(3) to 457.8 X 10(3) for LMW-BCGF. In 3H-TdR incorporation assays, 75% of cases showed a significant response to LMW-BCGF with a median stimulation index of 9.3. By comparison, only 33% of cases showed a significant response to HMW-BCGF with a median stimulation index of 2.4. Subsequently, B cell precursor colony assays were performed to assess and compare the biologic effects of BCGF on leukemic B lineage lymphoid progenitor cells. Among 28 cases studied, 57% responded to both HMW-BCGF and LMW-BCGF, 21% responded only to LMW-BCGF, and the remaining cases showed no proliferative response to either growth factor. The response patterns of virtually pure populations of FACS- sorted leukemic B cell precursors were essentially identical to the proliferative responses of unsorted leukemic B-cell precursors. Synergistic effects between HMW-BCGF and LMW-BCGF were observed in 80% of the cases that responded to both. The numbers of cell-bound radioiodinated BCGF molecules, the stimulation indices, as well as the number of B cell precursor colonies in BCGF-stimulated cultures showed a marked interpatient variation. Patients with structural chromosomal abnormalities (SCAs) involving 12p11-13 or patients with a Philadelphia chromosome showed a greater HMW-BCGF response at the level of leukemic progenitor cells than did other patients (P = .02). The LMW-BCGF response was significantly greater for patients with SCA than for patients without SCA (P = .04). The response of leukemic progenitor cells to HMW-BCGF or LMW-BCGF did not correlate with sex, age, disease status, FAB morphology, WBC at diagnosis, or immunophenotype. To our knowledge, this study represents the first detailed analyses of BCGF receptor expression and BCGF effects in B cell precursor ALL. The data presented provide direct evidence for the expression of functional receptors for both HMW-BCGF and LMW-BCGF in B cell precursor ALL.     

新疆维吾尔族空腹血糖受损人群血脂水平变化及低密度脂蛋白胆固醇升高的相关因素分析

目的 分析新疆维吾尔族（维族）IFG人群血脂代谢状况及LDL-C升高的危险因素。 方法 对新疆地区2053例30～80岁维族居民行横断面调查,筛查IFG人群,分析血脂代谢状况及LDL-C相关危险因素。 结果 该IFG人群中,血脂代谢异常的总患病率为99.8%（613/614）。高TG血症患病率为85.5%（525/614）,男女比较,差异无统计学意义（P〉0.05）;高TC血症、高LDL-C血症患病率为72.5%（445/614）和40.7%（250/614）,男性高于女性（75.0% vs 70.1%,52.4% vs 29.9%,P〈0.05）;低HDL-C血症患病率为29.8%（183/614）,男性低于女性（28.0% vs 31.4%,P〈0.05）。Logistic回归分析显示,LDL-C升高的危险因素为年龄、TC、2 hPG。 结论 维族IFG人群血脂代谢异常的总患病为99.8%,其中LDL-C升高的患病率为40.7%,其危险因素为年龄、TC和2 hPG。     

人肠道病毒71型感染免疫抑制恒河猴的分析

目的: 通过建立人肠道病毒71型(EV71)感染在免疫抑制恒河猴的动物模型,进一步分析人EV71在免疫抑制动物体内病毒增殖、病理变化以及病毒抗原在不同组织的表达情况。方法: 按照每天15 mg/kg的剂量给8只恒河猴口服环孢菌素A连续7 d后造成其免疫抑制状态,再经呼吸道和消化道感染EV71病毒FY23株,对其进行14 d的临床观察后处死,采集各器官组织进行病毒载量检测,同时对不同的组织进行病理学分析和免疫组化分析。结果: 感染EV71病毒后,免疫抑制组的临床症状、病毒载量、病理分析和免疫组化结果都明显比非免疫抑制组严重,从病毒对机体造成的影响来看呼吸系统感染较消化系统感染明显严重。结论: 本实验分析了人EV71在免疫抑制动物体内病毒增殖的情况,为建立用于EV71疫苗评价的恒河猴动物模型提供了依据,并对未来的EV71疫苗的使用人群范围做出了新的限定。     

人卵黄囊间质干细胞的分离纯化及诱导成脂的实验研究

目的:分离纯化及体外定向诱导人卵黄囊间质干细胞(YS-MSC)分化为脂肪细胞。方法:显微分离人卵黄囊, 经酶消化得到卵黄囊细胞, 卵黄囊细胞经贴壁培养、传代纯化得到人YS-MSC, 流式细胞仪检测YS-MSC表面抗原表达, 钙钴法测定碱性磷酸酶(AKP)活性;地塞米松、消炎痛、胰岛素定向诱导YS-MSC分化为脂肪细胞。油红O检测中性脂肪。结果:人卵黄囊间质干细胞易于分离、纯化, 体外培养增殖潜能大。卵黄囊间质干细胞CD29、CD44、CD166及CD105表达阳性, CD34、CD45和CD86为阴性;AKP弱阳性。卵黄囊间质干细胞经成脂诱导转化为大小不等的园形或椭圆形细胞, 可见胞浆内有少量微小脂滴形成, 随时间延长, 胞浆中脂滴相互融合, 胞核被挤于细胞的一侧, 经油红O染色脂滴染橘红色, 符合脂肪细胞的生物学特征。结论:人卵黄囊间质干细胞与成体间质干细胞表型一致, 在体外可以分化为脂肪细胞。     

学龄期喘息儿童雾化吸入全程行为干预的效果研究

目的 探讨雾化吸入全程中行为干预对学龄期喘息儿童的治疗效果的影响.方法 将141例学龄期喘息儿童于雾化过程中按是否自愿接受行为干预分为实验组75例和对照组66例,2组患儿雾化前均进行喘息知识认知和"呼吸"指导.实验组在此基础上由专科人员陪护整个吸入过程且进行"呼吸"、吞咽唾液或用鼻呼气、握持雾化罐、保持体位等行为干预.比较2组患儿雾化结束后肺部哮鸣音及患儿主诉是否改善、经皮测血氧饱和度以及患儿依从性的差异.结果 雾化完成后实验组患儿主诉较雾化前舒服73例占97.3%,SaO_2＞95%69例占92.0%;而对照组主诉较雾化前舒服53例占80.3%,SaO_2＞95%50例占75.8%,差异显著;实验组平静呼吸哮鸣音改善情况和医嘱依从情况与对照组相比差异显著.结论 雾化吸人全程中持续行为干预能显著提高喘息儿童雾化吸入的效果和吸入治疗依从性.     

原发性附睾肿瘤——附42例报告

目的探讨原发性附睾肿瘤的临床特点、病理类型及临床诊治方法。方法回顾性总结42例接受手术治疗的原发性附睾肿瘤患者临床资料。42例附睾肿瘤中良性肿瘤37例（88．1％），其中附睾腺瘤样瘤21例，平滑肌瘤9例，纤维假瘤2例，其余5例分别为乳头状囊腺瘤、血管平滑肌脂肪瘤、纤维瘤、纤维脂肪瘤和硬化性m管瘤。恶性肿瘤5例（11．9％），为附睾腺癌2例，非霍奇金淋巴瘤、横纹肌肉瘤和平滑肌瘤肉瘤变各1例。良性肿瘤作单纯肿瘤或患侧附睾切除，恶性肿瘤（除平滑肌瘤肉瘤变患者外）行根治眭附睾睾丸切除术，术后辅助放和／或化疗。结果良性肿瘤27例获随访，预后良好，术后无复发。恶性肿瘤5例均获随访，预后差，2例腺癌患者均于术后12个月死亡，横纹肌肉瘤患者术后20个月时广泛转移，非霍奇金淋巴瘤患者和平滑肌瘤肉瘤变患者健在。结论附睾肿瘤大部分为良性，手术切除效果好。恶性肿瘤少见，但预后较差，综合治疗可能会提高疗效。     

进展期结直肠癌动脉灌注新辅助化疗临床疗效观察

目的 探讨术前动脉灌注化疗对进展期结直肠癌的远期临床疗效.方法 选择ⅡB、Ⅲ期的结直肠癌患者128例,随机分为2组:试验组68例行术前动脉灌注化疗,方案为:奥沙利铂(艾恒)130mg/m2、羟基喜树碱20 mg/m2、氟脲苷600 mg/m2,经股动脉插管灌注化疗1、2次,8～14 d后接受手术治疗;对照组60例直接手术治疗.观察动脉灌注化疗不良反应及组织学疗效,比较2组手术切除率、手术并发症、术后病理分期及远期生存率.结果 试验组动脉灌注化疗不良反应主要表现为胃肠道反应和骨髓抑制,均属Ⅰ、Ⅱ度.试验组手术切除率为97.1%(66/68)、根治性切除率为96.9%,分别高于对照组的73.3%(44/60)和79.5%(x2=14.848、8.906,P均＜0.05);试验组组织学有效率达72.7%,病理分期均较术前降低,其中Ⅱ期病例明显较对照组增多(P＜0.05);试验组中位生存期为53.0个月,1、3、5年生存率分别为95.3%、85.9%、44.6%;对照组的中位生存期为42.0个月,1、3、5年生存率分别为92.6%、75.9%、22.0%,试验组生存率高于对照组,但只有5年生存率差异具有统计学意义(x2=6.385,P＜0.05).术后并发症2组比较差异无统计学意义(P＞0.05).结论 术前动脉灌注化疗对进展期结直肠癌降低临床分期、提高手术切除率尤其是根治性切除率作用和疗效确切,并能提高患者的远期生存率.

Abstract：

Objective To evaluate the curative effect of neoadjuvant chemotherapy via arterial infusion on advanced colorectal carcinoma. Methods One hundred and twenty-eight advanced colorectal carcinoma patients in stage Ⅱ B or Ⅲ were randomly divided into 2 groups. Sixty-eight cases received preoperative arterial infusion chemotherapy( the treatment group),and chemotherapy regimen consist of Oxaliplatin(L-OHP) 130 mg/m2, Hydroxycamptothecin (HCPT) 20 mg/m2 and Dexifluridine (FUDR)600 mg/m2. Femoral arterial infusion chemotherapy administrated 8 ～ 14 days preoperative. Sixty cases received surgery directly(the control group). The adverse reaction and histology effect after arterial infusion chemotherapy were observed, and resection rate,complications,pathology stage,together with long term survival were compared. Results Adverse reaction were mostly grade Ⅰ -Ⅱ gastrointestinal discomfort and bone marrow depression with arterial infusion chemotherapy. Resection rate was 97. 1% (66/68) ,and 64 cases(96. 9%) underwent raclical (R0) resection in the treatment group, which were higher than those in the the control group(73. 3%(44/60) and 79. 5%,respectively) (x2 = 14. 848,8. 906, Ps ＜ 0. 05). Histology effect of the treatment group was 72. 7%, and the pathology stage downstaged compared to preopeartion. Percent of patients in stage Ⅱ in the treatment group was higher than that in the control group( P ＜ 0. 05). The median survival time of test group was 53. 0 months, 1- ,3-,and 5-year survival rates were 95.3%,85.9% and 44.6%, respectively. In the control group, the median survival time was 42.0 months, 1-, 3-, and 5-year survival rates were 92.6%, 75.9% and 22.0%,respectively. There was significant difference in 5-year survival rate(x2 = 6. 385, P ＜ 0. 05). No difference in postoperative complications between two groups(P ＞ 0. 05). Conclusion The neoadjuvant chemotherapy via arterial infusion is of great significance on downstnging the pathology of advanced colorectal carcinoma, raising the excision rate, especially radical resection, and long term survival rate.     

实时组织弹性成像对甲状腺结节良恶性的鉴别诊断

目的 探讨实时组织弹性成像在甲状腺结节良恶性鉴别诊断中的应用价值.方法 术前使用超声弹性成像技术对80例,共165个甲状腺结节进行常规超声检查,并予以弹性成像分级.结果 结节性甲状腺肿、腺瘤、亚急性甲状腺炎弹性分级以≤Ⅱ级为主,甲状腺癌弹性以≥Ⅲ级为主.良性结节的弹性分级以0～Ⅱ级为主,恶性结节以Ⅲ～Ⅳ级为主,甲状腺良恶性结节弹性分级比较差异有统计学意义（P〈0.05）.弹性分级Ⅲ级时,良恶性结节比较差异有统计学意义（P〈0.05）.对于甲状腺结节良恶性的鉴别,超声弹性分级诊断的敏感度、特异度和准确率分别为100%、93.9%及94.5%.结论 实时组织弹性成像对鉴别甲状腺结节良恶性有着重要的应用价值.