密闭液性环境干燥环境下供皮区创面愈合的比较研究

目的比较研究密闭液性环境和干燥环境下供皮区创面的愈合过程. 方法 1996年7月～1997年3月以13例成人断层供皮区创面为研究对象,其中男9例,女4例,年龄20～50岁,创面面积(100～400) cm2,取皮深度中厚,约0.44 mm.同一个创面分为实验组(密闭液性环境)和对照组(干燥环境),采用自身对照法.应用大体、组织学及电镜观察等方法进行观察. 结果密闭液性环境下创面愈合较快,创面组织中成纤维细胞较早活跃、且持续时间较长,再血管化和再上皮化均较早和较快发生. 结论密闭液性环境下创面愈合较快,与组织修复细胞较为活跃、再血管化和再上皮化较快发生有关.     

传统腺样体切除术后腺样体残留情况调查

体统的腺样体手术为腺样体刮除术，为非直视下的手术，虽然多数患者手术后症状得得缓解，但临床上也常常看到手术后短时间内症状复发或者缓解不彻底的患儿，这类情况往往和腺样体手术切除不彻底有关。本院自2003年1月-2004年9月间对腺样体切除术后复发再次手术患者，以鼻内镜观察记录腺样体残留情况，对初次手术患者在进行传统腺样体刮除术并止血后，立即进行鼻内镜检查，了解腺样体残留情况并进行鼻内镜下处理，报告如下。     

ACTIVIT OF HUMAN PLACENTAL GAMMA GLOBULININ BLOCKING IMMUNE FUNCTIONS IN VITRO AND IN ABROGATING THE XENOGENEIC, LOCAL GRAFT-VERSUS-HOST-REEACTEON

In order to understand the mechanism of immunosuppression caused by infusion of placental gamma globulin (PGG) in patients with renal allografts, rheumatoid arthritis, and graft – versus –host disease (GCHD) following bone marrow transplantation (BMT) ,we have examined the effect of PGG in vitro and in a model of the xenogeneic , local graft –ver- sus – host reaction (LGVHR) .PGG inhibited lymphocyte proliferation in mixed lymphocyte cultures (MLC) (P<0.005) and depressed interleukin -2 (IL-2) levels in such cultures at 72 hours (P<0.01) . In contrast phytohemagglutinin (PHA) –and pokeweed mitogen (PWM) –induced T and B lymphocyte blastogenesis was not affected by such PGG treatment .PGG treatment .PGG neither decreased the [3H] TdR pulse incorporation in unstimulated lymphocytes nor affected cell viability .Cell cycle analysis by flow cytometry showed that PGG reduced the percentage of cells in S and G2, M phases during the MLC, but did not alter cell cycling during PWM-stimulated proliferation . An immunosuppressive effect of PGG on the LGCHR was tested in a model of intracutaneous transplantation of PGG –treat human lymphocytes into cyclophosphamide – immunosuppressed rats. Lymphoprep – separated human tonsillar lymphocytes were incubated with RPMI-1640 buffer containing：（1）PGG,4mg/ml,(2) human plasma albumin,4mg/ml,(3)mitomycin-C,25ug/ml, or (4) no additive. Cell of each preparation (3x107cells in 0.1ml) were injected intracutaneously into cyclophosphamide-treated male rats at separate abdominal locations. A fifth site received only the buffer solution. Five days after injection of cells ,each rat received [125 I]IUdR (10uCi) intraperitoneally and was killed after 5 hours. For each site of injection, the diameters of induration were measured and 125 I was counted . There was no difference between buffer – treated and a ibumin – treated groups either in the diameter of the area of induration (t=0.66;P>0.5)or in radioactive counts(t=0.22;P>0.05).In the PGG –treated group, the induration and radioactivity measurements were significantly less than in control groups (t=3.72 and P<0.1;t=2.62 and P<0.02,respectively ) Cytophilic antibodies in PGG were thought to inhibit an early phase of T cell activation, and not to be cytotoxicity .In the LGVHR, the immune response might be abrogated either by immuno- regulatory suppression of T cell function or by toxicity to the infused lymphoid cells. For some clinical purposes, immuno- modulating, human antibodies might be preferred to murine, monoclonal antibodies.     

100例2型糖尿病患者交感神经皮肤反应研究

目的探讨交感神经皮肤反应(SSR)检测在评价2型糖尿病(T2DM)自主神经损害中的价值.方法对100例T2DM患者进行SSR检测,30例健康志愿者作为对照.结果2组SSR的起始潜伏期、N波潜伏期、波幅、面积比较差异有显著性意义(P＜0.05),P波潜伏期差异无显著性意义(P＞0.05).T2DM组72例(72%)患者至少有一肢SSR异常.血糖控制满意组和血糖控制不良组比较,起始和N波潜伏期差异有统计学意义(P＜0.05),波幅和面积无显著性意义(P＞0.05).T2DM组病程＜5年与病程≥5年比较,潜伏期、波幅、面积差异均无统计学意义(P＞0.05).结论SSR可作为评价T2DM自主神经损害的客观电生理指标;T2DM患者SSR与血糖控制水平相关,与病程无关.     

Forty-six Cases of the Nerve Root-Involved Cervical Spondylopathy Treated by Needling the “Sitian” Points

In order to investigate the analgesic effect of needling at “Sitian” points for the nerve root-involved cervical spondylopathy, 68 cases of the nerve root-involved cervical spondylopathy were randomly divided into a treatment group of 46 cases treated by needling at ＂Sitian＂ points, and a control group of 22 cases treated by needling at cervical Jiaji points. After 2 therapeutic courses, the therapeutic effects were evaluated by using the visual analogue scale （VAS） and the semeiographic format. The results showed that the markedly effective rate was 78.3% and 54.5%, respectively in the treatment group and the control group, and the difference between the two groups was of significance （P〈0.05）. It can be concluded that needling at “Sitian” points can bring about a better therapeutic effect on the improvement of clinical symptoms, ohvsical signs and pain than that of the needling at the cervical Jiaii points.     

基于美军二战海战减员数据的校正

目的：通过对二战减员数据的校正，为下一步建立海战减员预计模型提供数据支持．方法：选取美军在二战太平洋海战中的减员数据作为研究的基本资料，通过专家咨询法(Delph method)对其进行校正，所有结果都对其进行了t检验．结果：水面舰艇遭受炸弹、舰炮及航空火炮、短程导弹、鱼雷和水雷的攻击时减员将减少，遭受中程导弹攻击时减员将会增加．结论：该校正结果可作为研究未来海战卫生减员预计的基本依据之一．     

桡腕关节重建治疗桡骨下端骨巨细胞瘤

目的探讨自体腓骨移植在桡腕关节重建术中的应用价值.方法应用带关节囊腓骨近端移植修复桡骨远端骨巨细胞瘤因瘤段切除后的骨缺损,游离移植4例,带血管蒂移植3例.结果所有被修复骨缺损均良好愈合,腕关节功能与外观令人满意.结论自体腓骨移植是修复桡骨远端缺损的理想供本,操作安全、有效、合并症少.     

再生丝素/NMMO·H2O溶液的流变性能

使用N-甲基吗啉-N-氧化物的水合物(NMMO·H2O)作为再生丝素的溶剂.可以得到w=0.10～0.25的再生丝素/NMMO·H2O溶液.研究了再生丝素/NMMO·H2O溶液流变性能,讨论了剪切速率、温度和溶液中的再生丝素的含量对再生丝素/NMMO·H2O溶液流变性能的影响.     

Ⅰ、Ⅲ型前胶原基因第2外显子核酶对靶RNA的体外切割活性的研究

目的 体外研究锤头型α1Ⅰ型及Ⅲ型前胶原基因第2外显子片段核酶对各自靶RNA分子的切割活性及反应条件。同时观察两反义核酶对瘢痕中成纤维细胞胶原合成的影响。方法 将含α1Ⅰ型及Ⅲ型前胶原基因第2外显子片段的重组质粒(pT-Ⅰ、pT-Ⅲ)，经体外^32P标记转录后形成产物靶RNA。同时将含特异性核酶基因的重组质粒(pT-gⅠ、pT-gⅢ)进行非标记的体外转录，产物(核酶)与各自的^32P-靶RNA按不同的条件混和反应，电泳后放射自显影观察结果。将构建好的核酶以脂质体包裹后导入培养的成纤维细胞内，采用图像分析法观察核酶对成纤维细胞Ⅰ型、Ⅲ型胶原蛋白mRNA合成的影响。结果 两种核酶在37℃、42℃均能有效切割各自的靶RNA，对Mg^2 浓度的要求范围较宽(10～20mmol／L)；反应温度从65℃逐渐降至并维持在37℃的条件下核酶切割活性显提高。Ⅰ、Ⅲ型胶原蛋白mRNA的表达明显降低，胶原蛋白生成降低，胶原生成明显受抑制。结论 针对α1Ⅰ型及Ⅲ型前胶原基因第2外显子片段的核酶能有效地在体外对靶RNA进行切割并能有效地抑制瘢痕中成纤维细胞胶原的合成。