Interleukin-6 and selected plasma proteins in healthy persons of different ages

In the present study, interleukin-6(IL-6) and several acute phase proteins were measured in healthy participants (23–87 years of age). A linear correlation between IL-6 and age was established with an increase of 0.016 pg/ml(00.004) per year of life. Whereas CRP remained below 0.5 mg/dl in all participants, an increase with age for fibrinogen and an inverse relation for albumin as well as transferrin were obtained. However, the increase of IL-6 did not correlate with any of these changes. IL-6 associated diseases may therefore occur more often with advancing age, but in healthy participants IL-6 does not explain the changing plasma protein pattern resembling that of an acute phase reaction.     

四物汤与十全大补汤的抗突变作用研究

用姐妹染色单体互换技术检测中药方剂四物汤、十全大补汤，发现其能明显降低环磷酰胺所致的ＳＣＥ值升高，表明有抗突变作用。     

Demonstration of pro-opiomelanocortin mRNA in pituitary adenomas and para-adenomatous gland in cushing's disease and Nelson's syndrome

Pro-opiomelanocortin (POMC) mRNA was demonstrated in pituitary adenomas from 16 patients with Cushing's disease and 10 with Nelson's syndrome. The intensity of signal was significantly greater in Nelson's syndrome than in Cushing's disease and there was a trend towards a greater proportion of positive cells. This probably reflects inhibition of POMC gene expression by the high circulating levels of cortisol in Cushing's disease. In the para-adenomatous gland, the intensity of signal was variable in cells showing Crooke's hyaline change, ranging from negative to strongly positive, in keeping with the functional heterogeneity of corticotrophs. In one case, junctional corticotrophs were present and these were more intensely stained than anterior lobe corticotrophs in the same gland. This supports the concept that these cells are subject to different regulatory influences from corticotrophs in the anterior lobe. Whether this is related to differences in embryological origins or to local factors is at present unclear.     

Progress in the treatment of congenital malformations of the hand

Treatment of congenital malformations of the hand and forearm has progressed not only from new procedures, but especially due to a new technique, namely microsurgery. Microsurgery enables skilled hand surgeons to operate on the tiny hands of babies without damaging the anatomical structures. The optimal time for the correction of many deformities is during the first 2 years of life. This gives excellent adaptation of the corrected structures to their altered function, especially in combination with a long period of further growth. Several new procedures are described which have been made possible only since the introduction of microsurgery, either by microvascular anastomoses or by meticulous dissection technique (toe transplantation, proximal toe phalanx transplantation, nail wall formation in complex syndactyly, transposition of digital parts in polydactyly and radial club hand).

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Resumen El tratamiento de las malformaciones congénitas de la mano y el antebrazo ha progresado no sólo en términos de nuevos procedimientos, sino, especialmente, en razón de una nueva técnica, la microcirugía. La microcirugía permite que los cirujanos de mano idoneos puedan operar sobre las pequeñas manos de bebés sin causar daño a las estructuras anatómicas. La época óptima para la corrección de muchas deformaciones es durante los 2 primeros años de vida. Esto hace posible una excelente adaptatión, de las estructuras que han sido corregidas a la función alterada, especialnmente durante períodos prolongados del crecimiento. Se describen algunos nuevos procedimientos que han sido factibles sólo desde la introducción de la microcirugía, bien sea por anastomosis microvascular o por una meticulosa técnica de disecci [trasplante de un artejo, transplante de la falange proximal, formación de la uña en sindatilia compleja, transposición de partes de dígitos en polidactilia y en mano zamba radial (club hand)].

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Résumé Le traitement des malformations congénitales de la main et de l'avant-bras a progressé non seulement grâce à de nouveaux procédés mais surtout grâce à l'apparition de toute une technique nouvelle: la microchirurgie. Celle-ci permet à des chirurgiens de la main expérimentés d'opérer les toutes petites mains des jeunes enfants sans compromettre la fonction des structures anatomiques. Les 2 premières années de la vie sont la meilleure période pour corriger la plupart des malformations: elles permettent une adaptation excellente des structures de fonction auparavant altérée, en se combinant avec la longue période de croissance à venir. Plusieurs procédés nouveaux sont répertoriés. Ils sont devenus possibles seulement depuis l'introduction de la microchirurgie: il s'agit notamment des anastomoses microvasculaires ou des techniques méticuleuses de dissection comme le transfert d'orteil ou de la phalange proximale de l'orteil, la création du mur latéral d'ongle dans un cas de syndactylie complexe ou la transposition de segments des doigts pour polydactylie ou main bote radiale.

     

Studies on the degenerative and regenerative phenomena occurring after transection and repair of the sciatic nerve in rats: effects of acetyl-L-carnitine

The effects of acetyl-L-carnitine on some degenerative and regenerative phenomena following sciatic nerve transection in rats, were studied. In Experiment 1, acetyl-L-carnitine was administered intraperitoneally at the dose of 50 mg/kg/day for 28 and 56 days following transection and microsurgical repair of the sciatic nerve. On day 56, the acetyl-L-carnitine-treated rats showed a significantly (p less than 0.05) better motor recovery ("clinical assessment") of the peroneal component of the sciatic nerve than the control rats. Twenty-eight days after nerve repair, the acetyl-L-carnitine-treated rats showed a significantly higher (p less than 0.05) number of myelinated axons in the postlesional nerve stump than control rats. Finally, the treated rats had a significantly lower (p less than 0.05) presence of atrophic fibres in the extensor digitorum longus muscle. In Experiment 2 the sciatic nerve was cut. To prevent spontaneous regeneration, a metallic clip was applied to the distal nerve stump and then the nerve stumps were positioned in different anatomical compartments. After surgery, a group of rats was treated with acetyl-L-carnitine dissolved in the drinking water (75 mg/kg/day). Another group of rats received normal water and served as the control group. Three, 6, 9, 12 and 18 months postoperatively, in the rats of both groups, the proximal sciatic nerve stump was injected with horseradish peroxidase to label the spinal cord neurons of the sciatic nerve nucleus. While in untreated rats the number of horseradish peroxidase-labelled neurons decreased with the increase in denervation time, in acetyl-L-carnitine-treated rats the number of horseradish peroxidase-labelled neurons remained stable for as long as 12 months of denervation and decreased only after 18 months of denervation. Furthermore, acetyl-L-carnitine-treated rats showed a significantly higher (p less than 0.05) number of horseradish peroxidase-labelled neurons with respect to untreated rats both after 9 and 12 months of denervation. In Experiment 3, the sciatic nerve was cut and then repaired after periods of 3, 6, 9, 12, and 18 months. Four months after nerve repair, the sciatic nerve was again cut and the proximal nerve stump was injected with horseradish peroxidase to label the spinal cord neurons of the sciatic nerve nucleus. Both acetyl-L-carnitine-treated and untreated rats showed a tendency to have an increased number of horseradish peroxidase-labelled neurons with respect to intact rats of correspondent ages.(ABSTRACT TRUNCATED AT 400 WORDS)     

正常人和脑梗死患者颈动脉血流超声多普勒时频分析

目的：分别对正常人和脑梗死患颈动脉血流超声多普勒时频分析,为临床应用提供参考资料。方法：检测正常人和脑梗患（大多有颈动脉粥样硬化斑块）的颈动脉多普勒血流时频谱,以其峰值频率（fmax),模式频率（fmode),平均频率（fmean),低值频率（fmin),重脉波频率（fp)和频弥散宽度（fw)为定量指标。结果：正常人颈动脉血流信号时频谱的fmax,fmean和fmode曲线在一个心动周期中具有总体相似的形状,相互嵌套,其中收缩期的fmax最高,均大于3kHz,fmin最低不低于1kHz,脑梗死患颈动脉时频谱的fmax与fmean和fmode两曲线分开,fmax减低（＜3kHz),fp消失,舒张末期fmin减低显（均低于0．5kHz),结论：颈动脉多普勒血流时频分析及所遴选的定量指标对颈动脉粥样硬化斑块十分敏感,可供诊断试验,且可集成在便携式计算机里,适用于社区人群中筛选易中风病变个体。     

神经移植端侧缝合颈5/7神经根与上干桥接治疗产瘫

目的：探讨一种不影响残存神经自行恢复的产瘫臂丛神经修复新方法。方法：将上干损伤部位行松解,不切除神经瘤,将其近端的颈5或颈7神经根和神经瘤远端的臂丛上干的神经束膜切开窗口,取颈丛皮支或前臂外侧皮神经前成多段（一般每段长约2．0－2．5cm）,两端分别与颈5神经根和上干的神经束膜行端侧缝合,结果：8例患儿经术后1－11年（平均3年）的随访,4例的三角肌和肱二头肌骨力达4级和4^-级,4例达3例。3例后期进行了肩关节松解和旋前圆肌肌腱切断,结论：该方法既提供了可使损伤近端颈5神经根的新生纤维生长至上干的神经通道,又未阻断神经瘤内残存神经纤维的自行恢复,是治疗产瘫特别是Tassin2型的新术式。     

子宫内膜癌和子宫内膜增生症中多种癌基因的表达

目的 :探讨癌基因、抑癌基因和抗转移基因蛋白在子宫内膜腺癌和子宫内膜增生症组织中的表达及意义。方法 :应用免疫组织化学染色 (SP法 ) ,对 6 6例子宫内膜腺癌和 30例子宫内膜增生症组织切片中的 p5 3、p2 1、c erbB 2、nm2 3基因产物表达进行检测。结果 :子宫内膜腺癌中 p5 3、p2 1、c erbB 2基因阳性率分别为 6 8.18%、71.12 %和 72 .73 % ,均高于子宫内膜增生症的 40 .0 0 %、2 0 .0 0 %和 46 .6 7% (P <0 .0 5～P <0 .0 0 5 )。腺癌中有 6 0 .6 1% (40例 )同时有多种基因表达 ,而nm2 3抗转移基因蛋白的表达与肿瘤分级有密切关系 (P <0 .0 1)。结论 :多种基因异常表达是判断子宫内膜腺癌预后的有效指标     

HNE1细胞株光动力学治疗前后形态学的动态变化

目的 探讨体外光动力学治疗（ＰＤＴ）引起鼻咽癌（ＮＰＣ）细胞死亡的作用机制。方法对鼻咽癌细胞株ＨＮＥ１体外光动力学治疗前后形态学变化进行动态观察。结果 对照组ＨＮＥ１细胞培养３ｈ,６ｈ,１２ｈ,２４ｈ和４８ｈ后细胞形态与正常培养组ＨＮＥ１细包开始变形皱缩,细胞界限模糊,细胞透光度和立体感较差,治疗２４～４８ｈ后细胞发生凋亡。结论 体外光动力学治疗对ＨＮＥ１具有明显的杀伤效应并ＨＮＥ１细胞凋亡。ＰＤ     

伴有糖尿病股骨颈骨折的治疗

目的探讨伴有糖尿病的股骨颈骨折的治疗方法.方法36例伴有糖尿病的股骨颈骨折患者通过三枚平行螺纹针内固定结合胰岛素或/和二甲双胍降血糖治疗.结果骨折愈合率为94.4%,优良率83.3%.结论该方法是治疗伴有糖尿病的股骨颈骨折患者的有效方法之一.