一种基于血管内皮细胞损伤的大鼠局灶性脑梗死模型

目的：建立一种因血管内皮细胞损伤而诱导的大鼠局灶性脑梗死模型。方法：取S—D大鼠11只，分离左侧颈总动脉、颈外动脉和颈内动脉。结扎左颈外动脉远心端，夹闭左翼腭动脉和颈总动脉，经颈外动脉逆行插管并缓慢注入月桂酸钠至颈内动脉系统，造成大脑中动脉血管内皮细胞受损。结果：从模型动物的行为表现、梗死灶形态学和病理学进行考察，10只大鼠均产生一定程度的脑梗死表现，造模成功率为90．9％。结论：该模型的稳定性和重复性较好，能在大脑中动脉的供血区产生恒定的梗死灶，适合进行脑缺血损伤的有关实验研究。     

Madigan前列腺切除术对前列腺增生症的治疗效果

①目的 提高前列腺增生症的手术治疗效果。②方法 采用Madigan手术治疗前列腺增生症42例。③结果 42例均手术成功，25例尿道完整，17例损伤尿道缝合修复；术后出血少，恢复快；术后随访4-85个月，病人无尿道狭窄，尿失禁等并发症。④结论 Madigan手术为较理想的前列腺增生症开放手术治疗方法。     

手助腹腔镜巨脾和胆囊联合切除术

目的 :总结手助的腹腔镜巨脾切除手术技术。方法 :用手助技术完成腹腔镜巨脾和胆囊联合切除术治疗 1例脾肿大、脾功能亢进伴胆囊结石患者。结果 :4 5h完成手术 ,术中出血较少。患者术后恢复良好。结论 :手助的腹腔镜巨脾和胆囊联合切除术操作安全 ,手术时间短 ,技术上完全可行     

The application of vitrification in human blastocyst cryopreservation: Analysis of clinical outcome of 117 cycles

Objective: To summarize the clinical outcomes of 117 human vitrified blastocyst transfer cycles and to determine the impact factors.Methods: In IVF-ET cycles, supernumerary embryos were cultured to 5-Day(D5) or 6-Day(D6), blastocysts of various stages were cryopreserved by vitrification using cryoloops. Survival rate and clinical pregnancy rate were observed.Results: A total of 312 blastocysts were thawed in 117 frozen embryo transfer cycles, the survival rate was 90.7% (283/312) after thawing. After the transfer of 230 blastocysts in 115 cycles, 69.6% (80/115) of the women got clinically pregnant, and 17.5% (14/80) of them suffered from miscarriage, 39 healthy babies were born in 28 deliveries, and the other 38 pregnancies are ongoing. The implantation rate was 47.4% (109/230). In 107 transfer cycles with 2 hatched blastocysts transferred in each cycle, 72.9% (78/107) got clinically pregnant, while in 8 cycles with 1 or no hatched blastocysts in the two transferred blastocysts, the clinical pregnancy rate is 25%(2/8). The clinical pregnancy rates were not statistically different between natural (77.4%, 24/31) or artificial endometrium preparation (66.7%, 56/84) cycles. Conclusions: These findings suggest that blastocyst vitrification is effective in terms of implantation rate and pregnancy outcome. Transferring of two hatched blastocyst can achieve a higher pregnancy rate.     

肾下型腹主动脉瘤的外科治疗

目的总结26例肾下型腹主动脉瘤的手术治疗经验。方法回顾性分析近5年多来手术治疗26例肾下型腹主动脉瘤的临床资料，全组26例，术前均经影像检查证实诊断。行择期手术21例，破裂型腹主动脉瘤急诊手术5例。26例均行腹主动脉瘤切除，人工血管重建术。结果围手术期死亡2例，均为急症手术患者，总病死率7．7％，急诊手术病死率40．0％。随访时间1-5年。术后1，3，5年生存率分别为96％，88％，75％。死亡原因均与腹主动脉瘤和手术无关。结论CTA检查是诊断腹主动脉瘤的可靠方法。手术治疗仍是治疗腹主动脉瘤的重要方法。瘤体直径不是决定手术的唯一指征。影响手术的危险因素主要是高龄、严重的心肺疾病和肾功能不全。     

虹吸原理在静脉穿刺中的应用

目的 :探讨虹吸原理在静脉穿刺中的应用 ,提高静脉穿刺的成功率。方法 :随机将 84例患者分为两组 ,一组用虹吸穿刺法 ,即将输液瓶放置在离穿刺部位 6 0cm左右的下方穿刺 ;一组用常规法。结果 :两组中用虹吸法立即回血占 88 1% ,用常规方法立即回血占 2 1 4 % ,差异有显著性 (P <0 0 5 )。结论 :此法提高了静脉穿刺成功率。     

二氮嗪对大鼠脑缺血再灌注细胞凋亡的影响

目的研究二氮嗪开放线粒体ATP敏感性钾通道对大鼠脑缺血再灌注细胞凋亡的影响。方法采用线栓法建立大鼠局灶性脑缺血再灌注损伤模型,将20只大鼠随机分成4组,假手术组、缺血组、缺血＋二氮嗪治疗组和缺血＋二氮嚷＋MitoK（ATP）通道特异性抑制剂5-HD组。观察各组凋亡细胞数和凋亡相关蛋白Bcl-2、Bax的变化。结果与缺血组比较,二氮嗪使凋亡细胞数明显减少（83．2±9．04 vs 123．96±13．45）,Bcl-2表达增高（0．17±0．01 vs 0．13±0．01）,Bax表达下降（0．15±0．02 vs 0．20±0．03）,差异具有显著性（P＜0．05）。5-HD能取消这些作用（P＜0．05）。结论局灶性脑缺血再灌注损伤时,二氮嗪能通过上调半暗带区Bcl-2蛋白表达,下调Bax蛋白表达,减少神经元凋亡,对脑缺血损伤起保护作用。     

MDR1特异性核酶逆转肝癌多药耐药的实验研究

目的探讨MDR1核酶（N2A＋tRNAi^met-iMDRl-sRz，sRz）在裸鼠体内逆转人肝癌组织多药耐药（MDR）的可行性。方法将原发性MDR人肝癌组织裸鼠原位移植模型第2代随机分为A组（空白对照组：生理盐水40μl＋Lipofect AMINE^TM2000 10μ1）、B组（阴性对照组：N2A＋tRNAi^met 10μg／40μl＋Lipofect AMINE^TM2000 10μl）和C组（核酶组：sRz 10μg／40μl＋LipofectAMINE^TM2000 10μl），均开腹瘤内注射。瘤内注药1周后用表阿霉素15mg／kg腹腔注射，每周1次，连续4周。彩色B超测量肿瘤体积。化疗结束后1周处死裸鼠，RT-PCR、Western blot法检测肿瘤中MDR1 mRNA及其蛋白P-gp的表达。结果C组每次化疗后肿瘤体积均较前缩小（F=659．99，P〈0．05）。除第1次化疗外，其余各次化疗后C组的抑制率均高于A、B组（F=35．36，12．77，97．60，P〈0．05）。化疗结束后，C组与瘤源以及A、B组相比，肿瘤组织中MDR1 mRNA和P-gp的表达明显降低（F=45．36，3590．40，P〈0．05）。结论sRz可有效降低肝癌细胞表达MDR1 mRNA和P-gp，一定程度上逆转MDR，提高E-ADM的化疗效果。单纯E-ADM化疗可使肝癌组织MDR1 mRNA和P-gp表达升高，诱导MDR的产生。     

Quick White美白胶漂白氟斑牙的临床效果

目的:临床观察QuickWhite美白胶对氟斑牙患者的漂白治疗效果。方法:选取63例I￣III级不同程度的氟斑牙患者,采用QuickWhite美白胶进行漂白治疗,分别于治疗前及治疗后用Vita比色板对牙齿比色并记录,评价治疗效果。评价标准:治疗后无改变为无效,变白2个色阶为有效,变白3个色阶以上为显效。结果:使用QuickWhite美白胶治疗后,I级患者显效率为95.6%,II级患者显效率为50%。结论:QuickWhite美白胶可为氟斑牙患者提供可靠的漂白治疗效果。     

Fumonisins as a possible contributory risk factor for primary liver cancer: A 3-year study of corn harvested in Haimen, China, by HPLC and ELISA

Employing HPLC fluorometry, gas-liquid chromatography (GLC) and a novel enzymelinked immunosorbent assay (ELISA) based on a monoclonal antibody, 40 corn samples, each collected in 1993 from agricultural stocks for human consumption in Haimen (Jiangsu County) and Penlai (Shandong Province), high- and low-risk areas for primary liver cancer (PLC) in China, respectively, were analysed for fumonisins (FBs), aflatoxins (AFs) and trichothecenes. Levels and positive rates of FBs and deoxynivalenol (DON) were significantly higher in Haimen than in Penlai. ELISA of the 40 corn samples harvested in the two areas in 1994 revealed that FB contamination levels and rates in these areas were comparable to those observed in 1993 in Haimen. ELISA analysis of 1993 and 1994 products revealed a wide occurrence of AFB₁ but the positive rates as well as levels were not significantly different between these areas. ELISA of the same sample number of corn harvested in 1995 revealed that FB contamination in Haimen was significantly higher than in Penlai. These 3-yearly surveys of corn samples (240 in total) demonstrated that corn harvested in Haimen was highly contaminated with FBs and that the contamination level, as well as positive rate in 1993 and 1995, were 10–50-fold higher than those in Penlai, suggesting FBs as a risk factor for promotion of PLC in endemic areas, along with the trichothecene DON. Co-contamination with AFs, potent hepatocarcinogens, was assumed to play an important role in the initiation of hepatocarcinogenesis.