糖尿病大鼠早期肾组织钙神经蛋白的表达

目的探讨糖尿病大鼠早期肾组织钙神经蛋白(Cal-cineurin,CaN)的表达及意义。方法大鼠随机分:对照组和糖尿病组。应用链脲佐菌素(65mg/kg)腹腔一次性注射方法建立大鼠糖尿病模型。4周末观察大鼠血糖、肾重/体重与尿白蛋白排泄率(AER)的变化。应用免疫组化与West-ern-blotting印迹检测肾组织CaN蛋白表达。结果糖尿病组大鼠血糖、肾重/体重与AER明显高于对照组。免疫组化显示糖尿病大鼠肾组织CaN蛋白表达较对照组明显增加(P<0.01),Western-blotting印迹显示糖尿病大鼠肾组织CaN蛋白表达较对照组增加2.4倍。结论糖尿病大鼠CaN过度表达表明糖尿病肾病发病过程可能与激活CaN信号转导途径有关。     

休闲驿站

开心一笑老股民股市大幅下跌,有个老股民手中股票全部跌停,非常烦躁。收盘后,他闷闷不乐地回到家。一进门,儿子就高兴地喊他:"爹!您回来了!"老股民     

降钙素原和超敏C反应蛋白联合检测在新生儿败血症早期诊断的价值研究

目的：探讨联合测定降钙素原（PCT）和超敏C 反应蛋白（hs-CRP）对新生儿败血症早期诊断和病情评估的应用价值。方法收集2011年11月至2013年5月期间收治于鄂尔多斯市中心医院的新生儿败血症患儿48例,记录患儿的 PCT、hs-CRP 及血培养等资料,并与同期48例非败血症新生儿的血清 PCT 和 hs-CRP 检测结果作对比分析。结果败血症患儿血清PCT（93．75％）、hs-CRP（10．42％）明显高于非败血症患儿血清 PCT（79．17％）、hs-CRP（50％）,两组间阳性率比较差异有统计学意义（P ＜0．05）。结论PCT 和 hs-CRP 在新生儿败血症早期有显著改变,血清 PCT 和 hs-CRP 的联合测定可以作为诊断新生儿败血症的早期诊断指标,且 PCT 的灵敏度和特异度均较 hs-CRP 高,两者的联合检测能为临床提供准确、快速的诊断依据。     

针刺对实验性糖尿病心血管并发症作用的病理学观察

本文用针刺治疗实验性糖尿病兔，观察针刺对其动脉粥样硬化病变及糖尿病性心肌病的影响，证明针刺对后者有一定的防治作用，并结合有关的功能指标探讨其机理。     

同病异证H22肝癌小鼠甲状腺机能及激素合成关键基因表达特征

目的：观察同病异证H22肝癌小鼠甲状腺机能及激素合成相关基因表达特征。方法：采用标准化诊法技术在接种同样H22腹水癌细胞数的昆明种小鼠中,筛选出不同组别的同病异证肿瘤小鼠,以ELISA法检测血清甲状腺激素水平,以RTqPCR法检测激素合成相关基因mRNA水平的改变。结果：①筛选出濒危组、邪毒组、邪中组、邪微组,及邪中兼气虚组（简称气虚组）等5个同病异证组小鼠。②甲状腺激素T3在肿瘤早期有升高趋势,邪盛衰度增加则出现下降;T4在肿瘤发生后迅速出现抑制。③激素合成相关基因Tshr、Tpo、Nis、Tg等基因转录水平总体表现出邪盛衰度低者表达量相对较高,高者表达量相对较低的趋势。结论：肿瘤发生后,邪盛衰度与荷瘤小鼠甲状腺机能抑制程度有关,邪毒微弱时呈代偿状态,加重后转而出现失代偿状态,是因实致虚,同时激素合成关键基因亦发生相应变化,从而影响甲状腺激素水平。     

长期饮酒对异丙酚使患者意识消失的半数有效效应室靶浓度的影响

目的 拟通过探讨长期饮酒对异丙酚使患者意识消失的半数有效效应室靶浓度(EC50)的影响,评价长期饮酒对异丙酚镇静效力的影响.方法 择期拟行外科手术的男性患者50例,年龄25～60岁,体重50～80kg,ASA分级Ⅰ或Ⅱ级,根据是否有长期饮酒史分为2组(n=25),对照组:日饮酒量＜25 g;长期饮酒组:日饮酒量超过45 g持续2年或2年以上.采用序贯法确定异丙酚使患者意识消失的EC50其95%可信区间,长期饮酒组和对照组异丙酚初始效应室靶浓度分别为2.0和1.5μg/ml,各相邻靶浓度之比为1.05.以睫毛反射消失及对言语指令无反应作为判断意识消失的标准.结果 长期饮酒组和对照组异丙酚使患者意识消失时的EC50及其95%可信区间分别为3.92(3.56～4.63)g/ml和2.73(2.26～3.31)μg/ml,长期饮酒组EC50高于对照组(P＜0.05).结论 长期饮酒可增加异丙酚使患者意识消失时的EC50,降低其镇静效力.

Abstract：

Objective To evaluate the effect of chronic alcohol intake on the sedative potency of propofol through investigating the effect of chronic alcohol intake on the half-effective target effect-site concentration ( EC50 )of propofol required for loss of consciousness in patients. Methods Fifty male ASA Ⅰ or Ⅱ patients, aged 25-60 yr, weighing 50-80 kg, scheduled for elective surgery, were divided into 2 groups according to the history of chronic alcoholic intake ( n = 25 each): control group (alcoholic intake per day ＜ 25 g) and chronic alcoholic group (alcoholic intake per day＞45 g, lasting for 2 yr or more). The EC50 and 95% confidence interval (CI)were determined by up-and-down sequential method. The initial target effect-site concentration was 2 μg/nl in chronic alcoholic group and 1.5μg/ml in control group, and the ratio between the two successive concentrations was 1.05. Loss of consciousness was defined as loss of response to verbal command and eyelash stimulation. Results The EC50 of propofol that produced loss of consciousness was 3.92 (95 % CI 3.56-4.63 ) μg/ml in chronic alcoholic group and 2.73 (95%CI 2.26-3.31)μg/ml in control group. The EC50 of propofol was significantly higher in chronic alcoholic group than in control group ( P ＜ 0.05). Conclusion Chronic alcohol intake can increase the EC50 of propofol required to induce loss of consciousness and reduce sedative potency in patients.     

基于免疫磁珠法的自动化粪便脱落细胞提取仪的研制与应用

Objective To design a device based on immunobead method which enables extraction of exfoliated colonocytes from human feces to become an automatic concentrating process. Methods The automatic device for extraction of exfoliated colonocytes from human feces was designed based on the composition and physiochemical features of the feces as well as the distribution of exfoliated colonocytes, so as to integrate its functions in sample collection, pretreatment, exfoliated colonocyte-immunobead binding,and exfoliated colonocyte- immunobead complex washing. The immunobeads were prepared by coating magnetic beads with monoclonal antibody of epethelial cell adhesion molecule (EpCAM) Ber-EP4 and used to concentrate colorectal cancer cells from cell culture. The binding of colorectal cells to immunobeads was observed under microscopy. Cancer tissues of 10 colorectal cancer patients (or tissues around cancer) were collected. The automatic device for extraction of exfoliated colonocytes from human feces was used to get exfoliated cells from stool specimens of the patients. QRT-PCR was used to detect gene expression of c-myc、cox-2、CD44v6 in tissues and cells collected. Rate of gene positive expression of different original specimens was compared. Results The automatic device for extraction of colonocytes from human feces was designed and manufactured. Extracting exfoliated coloncytes from feces sample was successfully completed by the automatic device and self-prepared immunobeads with coating special monoclonal antibody. The recovery rate of cells was 27%. Rate of c-myc, cox-2 and CD44v6 gene positive expression in cancer tissues or tissues around cancer was 20%, 10% and 20% respectively, and 100% in exfoliated cells from stool specimens. The difference of positive rate among specimens was significant (all P＜0.01). Exfoliated cells from stool specimens collected by the automatic device for extraction of exfoliated colonocytes from human feces were cancer cells. Conclusions As one of the latest-developed approaches, immunobead-based concentration of exfoliated colonocytes appears practical. An automatic device thereby developed may be used for early detection of colorectal cancers.     

云南省应对澜湄流域毒品犯罪问题研究

毒源地"金三角"地理位置处于"澜沧江—湄公河"流域的中心地带,受全球化国际毒潮泛滥的影响,澜湄流域毒情形势严峻复杂,传统毒品与合成毒品的种、制、贩活动与消费供应均呈现出不减反增的态势,并且跨境有组织的毒品犯罪活动频发高发,破坏了"澜湄六国"的安全稳定和国际形象.云南作为澜湄流域禁毒执法安全合作的关键枢纽,不仅要应对流域...