全文获取类型
收费全文 | 715篇 |
免费 | 31篇 |
国内免费 | 24篇 |
专业分类
耳鼻咽喉 | 6篇 |
妇产科学 | 3篇 |
基础医学 | 34篇 |
口腔科学 | 16篇 |
临床医学 | 112篇 |
内科学 | 67篇 |
皮肤病学 | 7篇 |
神经病学 | 44篇 |
特种医学 | 23篇 |
外科学 | 32篇 |
综合类 | 216篇 |
预防医学 | 42篇 |
眼科学 | 21篇 |
药学 | 70篇 |
3篇 | |
中国医学 | 57篇 |
肿瘤学 | 17篇 |
出版年
2024年 | 1篇 |
2023年 | 18篇 |
2022年 | 25篇 |
2021年 | 29篇 |
2020年 | 31篇 |
2019年 | 27篇 |
2018年 | 23篇 |
2017年 | 9篇 |
2016年 | 28篇 |
2015年 | 13篇 |
2014年 | 42篇 |
2013年 | 45篇 |
2012年 | 38篇 |
2011年 | 47篇 |
2010年 | 49篇 |
2009年 | 41篇 |
2008年 | 44篇 |
2007年 | 39篇 |
2006年 | 46篇 |
2005年 | 33篇 |
2004年 | 27篇 |
2003年 | 29篇 |
2002年 | 19篇 |
2001年 | 15篇 |
2000年 | 21篇 |
1999年 | 3篇 |
1998年 | 7篇 |
1997年 | 5篇 |
1996年 | 4篇 |
1995年 | 5篇 |
1994年 | 1篇 |
1993年 | 4篇 |
1992年 | 2篇 |
排序方式: 共有770条查询结果,搜索用时 15 毫秒
51.
52.
采用毛细管柱定量法测定壬代环戊双醚,结果:线性范围为0.28~15 μg,线性方程Y=1.7015×105 0.086562×107 X,平均回收率为99.98%,满足定量分析的要求. 相似文献
53.
54.
气管切开患者鼻饲致食物反流的原因分析和护理对策 总被引:4,自引:0,他引:4
由于脑部的各种疾患,神经外科患者会有不同程度的意识障碍.此类患者常会出现呼吸道及脑缺氧等并发症,常采用气管切开,使这些患者不能经口进食,需进行鼻饲饮食. 相似文献
55.
经鼻内镜下改良鼻咽血管纤维瘤切除术 总被引:3,自引:1,他引:3
如何减少经鼻内镜下鼻咽纤维血管瘤切除术的出血量,缩短手术时间,对该手术的推广非常重要,我们引对此点进行了改进。 相似文献
56.
57.
Objective: To evaluate the differentiation of human umbilical cord blood cells into hepatocyte-like cells. Methods: Mononuclear cells (MNCs) derived from human umbilical cord blood were isolated using Ficoll. The experiment was derived into 3 categories:(1) MNCs co-cultured with 50 mg minced liver tissue separated by a trans-well membrane and then collected at 0 h,24 h,48 h and 72 h; (2) MNCs cultured along supplemented with 100 ml/L FBS. 100μ/ml penicillin, 100μg/ml streptomycin. 4. 7μg/ml linoleic acid, 1×ITS, 10-4 mol/L L-Ascorbic acid 2-P and a combination of FGF4 (100 ng/ml) and HGF (20 ng/mL). Cells were then collected at 0 d and 16 d to examine the expression profile of hepatocyte correlating markers;(3) 0. 2-0. 3 ml of MNCs with a cell density of 2×107/ml were transplanted into prepared recipient mice [n = 12, injected with 0. 4 ml/kg (20%) CCl4 and 150 ng/kg 5-fluorouracil (5-Fu) prior the transplant 24 h and 48 h, respectively] via injection through tail vein. Mice were sacrificed 4 weeks after transplantation. The hepatocyte correlating mRNAs and proteins were determined by RT-PCR, immunohistochemical analysis and immunoflurence technique. Results: (1) After 72 h. a number of glycogen positive stained cells were observed with MNCs co-cultured with damaged mouse liver tissues. The expression of hepatocyte markers, human albumin (ALB),α-fetal protein (AFP) and human GATA4 mRNA and proteins were detected by RT-PCR and immunohistochemistry as well. For the confirmation, the DNA sequencing of PCR products was performed. In control groups, MNCs co-cultured with normal mouse hepatocytes or MNCs cultured alone, all markers remained negative. (2) In growth factor supplemented culture system, MNCs developed into larger volume with richer cytoplasm and binucleation after 16 d. Positive expression of ALB, AFP, CK18 and CK19 mRNA were detected with RT-PCR, and ALB positive staining was observed by immunocytochemistry as well. In contrast. MNCs cultured without exogenous growth factors scarcely attached to the culture dish and ALB mRNA was not detected. (3) In transplantation experiment, both of ALB and AFP mRNA were detected by RT-PCR and HSA, PCNA and ALB positive staining were observed in the livers of recipient mice by immunocytochemistry. Conclusion: MNCs from human umbilical cord blood could convert into hepatocyte-like cells in 3 different ways, indicating their potential use in the clinic applications for the treatment of human liver diseases. 相似文献
58.
59.
目的 探讨减少和防止肛周尖锐湿疣电离子治疗术后感染和复发的方法.方法 肛周尖锐湿疣患者共102例,分为治疗组72例,对照组30例.对照组使用CHR-IIB型多功能治疗仪,术后创面用红霉素软膏涂擦;治疗组除对照组方法外,结合术后中药熏蒸坐浴,每日1次,连续7~10 d为1个疗程.结果 治疗组有效率94.4%,感染率1.4%,复发率2.8%;对照组有效率70.0%,感染率33.3%,复发率33.3%.结论 电离子治疗仪治疗肛周尖锐湿疣术后结合中药熏蒸坐浴治疗疗效较好,感染率和复发率低. 相似文献
60.