Highly resolving two-dimensional gels for protein sequencing.

Two-dimensional (2D) PAGE, using carrier ampholytes for the first-dimension separation, has provided a tool for the simultaneous analysis of cellular proteins. To extend the utility of 2D PAGE to the preparative level, we have investigated the use of immobilized pH gradients (IPG) for the first-dimension separation. The results we have obtained indicate that as much as 1 mg of cellular protein can be loaded onto a single IPG gel without loss of resolution. Mutant polypeptides previously detected in carrier ampholyte-based 2D gels were equally detectable in IPG-based 2D gels. With IPG gels several hundred cellular polypeptides can be isolated, from as few as 10 gels, in sufficient amount for sequencing with current sequencing technology. We therefore conclude that IPG greatly enhances the prospects for the large-scale sequencing of cellular proteins for the development of 2D gel-related protein data bases and for the identification of new polypeptide gene products, with the attendant implications for a genome sequencing effort.     

中国饮用水源水中藻类卫生标准的研究

为制定饮用水源中藻类限值 ,结合国内外文献调查研究毒理学试验和现场生态学调查资料 ,根据世界卫生组织 (WHO)和卫生部推荐的饮用水中微囊藻毒素MC -LR的限值、藻类生物量和MC -LR浓度关系以及毒理学实验所得到的藻类的无作用浓度而制定饮用水源中藻类限值。结果显示 ,推荐饮用水源中藻类三级限值分别为 :安全限值 :1.0× 10 4个 升 ;警戒限值 :2 .1× 10 5个 升 ;危险限值 :1.2× 10 6 个 升。提示饮用水源中藻类限值在日常工作中有显著意义和可行性     

神效止痛膏的镇痛作用

用二甲苯所致的急性炎症模型观察了神效止痛膏的抗急性炎症作用．用扭体法、热板法观察了神效止痛膏对小鼠的镇痛作用．结果表明，神效止痛膏有很好的镇痛作用．     

硫酸镍对红细胞毒性研究

健康小鼠腹腔注射硫酸镍10.0,5.0,2.5mg/kg,导致外周血中红细胞增高,血红蛋白不变或降低,红细胞中点彩红细胞和碱粒红细胞占有比值明显增高,网织红细胞含量升高、提示镍对外周血红细胞有毒性损害。     

胆囊切除术引流与不引流200例临床分析

胆囊切除术后是否行腹腔引流，外科医生意见不一．本文通过对２００例胆囊切除术的对比分析，总结了“引流”与“不引流”的利弊和经验。认为择期单纯胆囊切除不必常规放置引流，但对手术后有可能发生出血、胆漏的病例，在放与不放引流犹豫不决时，仍以放置引流为妥．本文同时提出了胆囊切除术引流与不引流的适应症．     

PHAGAQUOSONOGRAM (PASG) DIAGNOSIS FOR CANCER OF GASTRIC CARDIA A PRELIMINARY STUDY OF 223 PATIENTS

Phagaquosonographies (PASG) of 100 normal subjects and 223 patients with cardiac cancer (CC) were analyzed. The apparatus was self-designed and self-manufactured. The wave patterns and the pre-ejection period and other 8 quantitative indices in PASG were compared and patients with CC were found to have abnormal levels (P less than 0.01). The phagaquosonograms in 82 patients with esophageal, gastric or cardias (non-cancer) diseases served as controls. The PASG showed positive results in 97.3%, suspicious in 2.3% and negative in 0.4% of CC cases and 100% negative in the normal subjects. It was 1.2% positive, 8.5% suspicious and 90.3% negative in the esophageal, cardiac or gastric non-cancer diseases. PASG has high sensitivity (97.3%), and typical characteristics (98.8%). It is shown that this method can be used in screening, diagnosis, differential diagnosis and prediction of prognosis of cardiac cancer.     

中美护理本科《护理学基础》课程设置的比较研究

[目的]对中美护理本科《护理学基础》课程设置情况进行比较,为中国护理本科《护理学基础》课程改革提供可借鉴的科学依据。[方法]选取美国和中国各20所全日制护理院校对其护理专业本科课程《护理学基础》的开设情况进行统计分析。[结果]美国护理本科有65.00%的院校开设《护理学基础》,其实践学时和理论学时数分别为30学时~135学时和20学时~56学时,两者比值为1.00~3.00;中国护理本科院校均开设《护理学基础》,其实践学时和理论学时数分别为18学时~268学时和54学时~128学时,两者比值为0.33~2.31。[结论]中美护理本科《护理学基础》课程开设情况存在一定差异,我国《护理学基础》有内容较简单、操作多、理论少、学时数较多特点;而美国《护理学基础》学时数较少,实践学时所占比例较大。     

哌泊塞嗪棕榈酸酯与氟哌啶醇癸酸酯治疗精神分裂症的对比

精神分裂症病人６１例随机双盲分为哌泊塞嗓组（男性２５例，女性６例，年龄４０±ｓ９ａ，每４ｗｋｉｍ１次，平均剂量１１±３５ｍｇ／４ｗｋ，共６次）和氟哌啶醇组（男性２４例，女性６例，年龄３８±９ａ，每４ｗｋｉｍ１次，平均剂量１２０±２８ｍｇ／４ｗｋ共６次）。结果：ＢＰＲＳ减分率、总显效率、生效时间２组差别无显著意义。     

中老年人24小时动态血压变化的研究

目的探讨血压正常老年患者和老年高血压患者24h动态血压波动规律及与靶器官损害的关系.方法采用无创伤袖带式动态血压监测仪检测36例中年人(对照组),50例血压正常老年患者,60例老年高血压患者的动态血压.结果血压正常老年患者较中年血压正常者24h动态血压呈杓型曲线的比例明显下降.血压正常老年患者,血压昼夜波动曲线与心、脑、肾损害无相关性.老年高血压患者,血压曲线呈非杓型者比例增高,血压昼夜节律消失较节律正常的高血压患者有更显著的靶器官损害.结论对于非高血压患者,随年龄的增加,血压昼夜节律消失者增多,血压曲线变化与心、脑、肾损害无关,可能与原发病有关.对于高血压患者,血压昼夜节律紊乱可作为靶器官损害的预测因子.