A rat model of bone inflammation-induced pain by intra-tibial complete Freund's adjuvant injection

In prior studies, models of inflammatory pain were produced through injecting complete Freund's adjuvant (CFA) or capsaicin directly into either the deep somatic tissue or the animal's hind paw. In contrast, bone cancer-induced pain (BCIP) was simulated through injecting tumor cells into the cavity of the femur or the tibia. It has been reported that, due to differences in afferent innervation, the same stimulus to various tissue types might result in differing patterns of pain response. Hence, the aim of this study is to establish a rat model of bone inflammation-induced pain (BIIP) by injecting CFA into the tibial cavity, the same site involved in the BCIP model. The differences in body weight, bone histology, mechanical allodynia, thermal hyperalgesia, and the pain relieving effects of Celebrex on this model of BIIP were evaluated. The results showed that there was evidence of significant inflammation seen in the bone marrow two days after intra-tibial CFA injection, including nuclear condensation and fragmentation, massive neutrophilic granulocytes, and prominent fibrinous exudates. Fourteen days after injection, marked fibrosis of the bone was detected by histological staining. After unilateral CFA injection, behavioral studies showed mechanical allodynia to von Frey hair stimulation, but no thermal hyperalgesia was observed. Celebrex showed significant anti-allodynic effects on the BIIP model. The results demonstrated that CFA is an effective agent for inducing bone inflammation and subsequent pain-related behavior in rat models, and, thus, provides a practical and valuable contrast for BCIP research.     

阴道镜检查280例临床分析

目的评价阴道镜检查在宫颈病变诊断中的价值。方法2004年12月-2005年6月我院对有宫颈病变的280例门诊病人进行阴道镜检查并以改良Reid评分，其中有218例同时接受病理检查。结果病理确诊为宫颈不典型增生（CINⅠ～Ⅲ）8例，其中CINⅠ4例，CINⅡ3例，CINⅢ1例；宫颈原位癌1例、宫颈浸润癌1例、慢性宫颈炎113例、宫颈息肉80例及HPV感染者15例。与阴道镜检查结果对照，浸润癌1例在镜下诊断癌疑，1例原位癌及8例CIN的改良评分为1—5分，而慢性宫颈炎则为0～1分。结论阴道镜检查对宫颈癌、宫颈癌前病变、HPV感染的早期诊断具有较好的筛查作用，但由于评价的标准包含着一定的主观因素，因而不能作为确诊依据。     

慢性湿疹皮炎患者斑贴试验的护理122例

目的 探讨慢性湿疹皮炎斑贴试验的护理要点。方法 对122例慢性湿疹皮炎的临床资料、护理措施进行分析。结果 居常见致敏原前5位的化学物质分别是0．1％硫柳汞、5％硫酸镍、7％芳香混和物、1％甲醛（水溶液）、1％肉桂醇。结论 严格执行班贴试验前、试验中以及试验后的注意事项是取得斑贴试验成功的关键；注意对息者斑贴试验结果的解释和对患者进行生活护理。     

Immunogenicity of polyepitope libraries assembled by epitope shuffling: an approach to the development of chimeric gene vaccination against malaria

Developing a polyepitope vaccine which contains diverse antigenic types is a promising strategy to cope with the problem of malaria variation and diversity. However, arranging the peptides to produce the most effective immunogenicity remains a hurdle. In an attempt to develop an effective complex antigenic gene vaccine, we constructed a polyepitope library by randomly assembling epitopes using the epitope shuffling technique. The polyepitope library, which contains epitopes from different antigens of Plasmodium falciparum, was divided into five sub-libraries based on the size of chimeric genes. Here we report that higher antibody titers were observed in mice with immunized with sub-libraries containing genes >1200 bp, using enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence (IFAT) assay to determine both individual epitope peptides and the natural parasites at the erythrocyte stage. Different levels of IgG subtypes and cytokines were elicited by different sub-library and administration times. In a rodent malaria model, some groups of immunized mice were partially cross-protected against a lethal challenge from Plasmodium yoelii. These results suggest that the immunogenicity of a polyepitope chimeric antigen is essentially conformation- and length-dependent, and demonstrates that the promising advantage of epitope shuffling technology is that it allows us to randomly assemble many polyepitope molecules in tandem format. This finding also indicates that polyepitope library vaccination is a suitable approach for screening optimized chimeric gene vaccines against malaria and other diseases.     

在无血清培养基中代谢副产物对杂交瘤细胞生长代谢的影响Ⅲ.丙氨酸的作用

研究了无血清培养中的代谢副产物丙氨酸对杂交瘤细胞生长代谢的影响。丙氨酸浓度在0～ 8.9mmol/L范围时 ,丙氨酸对细胞生长影响不大 ,对单抗的生成没有直接影响 ,但对细胞代谢有较大的影响。随着丙氨酸浓度的提高 ,葡萄糖的消耗增加 ;同时 ,乳酸的生成增加 ,而丙氨酸和氨的生成减少     

纤维素酶法提取茶多糖

为了保持茶多糖的活性 ,采用低温水提、酶提二次结合法提取茶多糖 .第一次在 5 0℃、茶叶与水的质量比为 1∶1 5、水提取 3 0min ,多糖的提取率为 2 .3 3 % ,粗多糖 (干重 )的提取率为6.82 % ;过滤后 ,滤渣用 pH 4.6的柠檬酸 柠檬酸钠缓冲液加纤维素酶提取 ,经正交试验确定酶提最佳工艺参数为 5 5℃、茶叶与水的质量比为 1∶1 4、酶用量 2 .2 μL/g(以茶叶质量计 ) ,反应时间为1 2 0min ,其多糖的提取率为 0 .64% ,粗多糖 (干重 )的提取率为 1 .1 1 % ,分别占二次总提取量的2 1 .5 %和 1 4.0 % ,而在相同条件下无酶提取 ,提取率仅为 0 .3 9%和 0 .5 6% .相对水提法 ,酶法的多糖提取率分别增加 63 .3 %和 98.9% ,多糖总提取率达 2 .97% ,粗多糖 (干重 )的总提取率为7.93 % .采用酶法提取的茶多糖具有较强抑制α 淀粉酶活力的能力 .     

动脉吻合术后弹性纤维的扫描电镜观察

目的　研究动脉吻合术后弹性纤维的构筑。　方法　在手术显微镜下将 Wistar大鼠 60只右侧股动脉横行切断后行端端吻合术 ,于手术后 3、7、1 4、2 1、30和 90天切取吻合侧动脉及对侧动脉 ,经 1 0 %中性甲醛溶液固定 ,将动脉段纵行中间切开 ,置于 90 %甲酸中 ,45℃消化 96小时 ,液氮速冻 ,置真空蒸发器中干燥 2 4小时 ,取出真空镀金后在扫描电镜下观察弹性纤维在动脉吻合术后的重构。　结果　动脉吻合术后 3、7天吻合口中心弹性纤维缺失 ,术后 1 4～90天 ,新生弹性纤维由新生内膜部位向吻合口中心延续。弹性纤维的重构分为 :静止期、增生期、重建期。　结论　动脉壁的弹性纤维在术后经历了重建过程 ,30天弹性纤维的形态结构趋于正常。弹性纤维的修复为新生内膜型     

心脏瓣膜手术前后血浆血小板因子4和β血小板球蛋白改变的临床研究

对３０例心脏瓣膜患者手术前后血浆血小板因子４（ＰＦ４）和β血小板球蛋白（βＴＧ）浓度测定显示，术前心房颤动病例的ＰＦ４和βＴＧ浓度高于窦性心率病例，瓣膜替换病例术后ＰＦ４和βＴＧ高于单纯二尖瓣闭式扩张病例。本文提出，术前心房颤动病例及瓣膜替换术后病例的体内血小板处于活化状态，具有血栓形成倾向，并对ＰＦ４和βＴＧ升高的成因及其临床意义进行讨论。     

复发性卵巢交界性肿瘤保留生育功能手术的临床疗效观察

目的:了解复发性卵巢交界性肿瘤患者施行保留生育功能手术后的月经、生育功能以及肿瘤复发情况.方法:回顾分析2000至2006年浙江大学附属妇产科医院收治的复发性卵巢交界性肿瘤6例患者施行保留生育功能手术后的月经、生育以及复发情况.结果:(1)6例患者中5例月经正常;(2)3例患者共妊娠4次,其中3次为足月妊娠并分娩活婴,1次流产,自然妊娠率达50%;(3)平均随访时间为32个月(6～72个月),其中1例复发,但未发生与肿瘤相关的死亡.结论:对年轻有强烈生育要求的复发性卵巢交界性肿瘤患者仍可考虑施行保留生育功能手术,术后自然妊娠率高,但需严密随访.     

21世纪是人类战胜癌症的时代

通过对近年来坚持以中医理论为指导,从整体出发辨证论治中晚期癌症经验的总结,认为癌症的共性是“局部为实,整体为虚”,其整体调治的四大原则为：攻局部之实,补整体之虚,调全身阴阳,并配合以心理疏导,可以提高癌症患者生存质量,乃至达到康复的目的。