全文获取类型
收费全文 | 3863篇 |
免费 | 166篇 |
国内免费 | 38篇 |
专业分类
耳鼻咽喉 | 20篇 |
儿科学 | 49篇 |
妇产科学 | 186篇 |
基础医学 | 499篇 |
口腔科学 | 73篇 |
临床医学 | 330篇 |
内科学 | 706篇 |
皮肤病学 | 83篇 |
神经病学 | 421篇 |
特种医学 | 156篇 |
外科学 | 440篇 |
综合类 | 30篇 |
预防医学 | 350篇 |
眼科学 | 137篇 |
药学 | 338篇 |
中国医学 | 2篇 |
肿瘤学 | 247篇 |
出版年
2023年 | 13篇 |
2022年 | 39篇 |
2021年 | 99篇 |
2020年 | 46篇 |
2019年 | 64篇 |
2018年 | 87篇 |
2017年 | 60篇 |
2016年 | 74篇 |
2015年 | 78篇 |
2014年 | 118篇 |
2013年 | 152篇 |
2012年 | 305篇 |
2011年 | 279篇 |
2010年 | 158篇 |
2009年 | 139篇 |
2008年 | 255篇 |
2007年 | 233篇 |
2006年 | 239篇 |
2005年 | 241篇 |
2004年 | 228篇 |
2003年 | 205篇 |
2002年 | 180篇 |
2001年 | 65篇 |
2000年 | 69篇 |
1999年 | 55篇 |
1998年 | 44篇 |
1997年 | 28篇 |
1996年 | 18篇 |
1995年 | 27篇 |
1994年 | 16篇 |
1993年 | 19篇 |
1992年 | 47篇 |
1991年 | 30篇 |
1990年 | 27篇 |
1989年 | 28篇 |
1988年 | 30篇 |
1987年 | 30篇 |
1986年 | 33篇 |
1985年 | 15篇 |
1984年 | 21篇 |
1983年 | 18篇 |
1982年 | 13篇 |
1980年 | 8篇 |
1979年 | 13篇 |
1978年 | 12篇 |
1977年 | 9篇 |
1976年 | 13篇 |
1973年 | 15篇 |
1972年 | 13篇 |
1970年 | 10篇 |
排序方式: 共有4067条查询结果,搜索用时 15 毫秒
991.
mRNA expression, functional profiling and multivariate classification of colon biopsy specimen by cDNA overall glass microarray 总被引:1,自引:0,他引:1
Galamb O Sipos F Dinya E Spisak S Tulassay Z Molnar B 《World journal of gastroenterology : WJG》2006,12(43):6998-7006
INTRODUCTION mRNA expression array analysis is usually performed on high volume surgery or blood samples. However, evaluation of routine biopsy specimens could yield information, as to how the local pathological processes differ from healthy counterparts.… 相似文献
992.
Toth PP 《Journal of clinical lipidology》2010,4(5):376-381
The proteosome of high-density lipoprotein particles is quite complex and consists of up to 75 different proteins and enzymes. The specific protein cargo of HDL particles regulates their functionality. In addition to their documented capacity to engage in reverse cholesterol transport, reduce oxidized lipid, and function as apoprotein donors, HDL particles can activate a variety of signaling systems in endothelial cells, smooth muscle cells, and platelets. The HDLs can deliver sphingolipids to the surface of these cell types and activate sphingosine phosphate receptors. Sphingosine phosphate receptors are coupled to numerous different intracellular signaling cascades exerting roles in vasodilatation, inflammation, cell migration and apoptosis, inhibition of platelet activation, and endothelial adhesion molecule expression, among other functions. The ability of HDL to influence such a diverse array of cellular functions lends biological plausibility to the substantial epidemiological and clinical evidence suggesting that the HDLs are unique among lipoproteins in that they are vasculoprotective and antiatherogenic. 相似文献
993.
Ficsor L Varga V Berczi L Miheller P Tagscherer A Wu ML Tulassay Z Molnar B 《Cytometry. Part B, Clinical cytometry》2006,70(6):423-431
BACKGROUND: Automated virtual microscopy of specimens from gastrointestinal biopsies is based on cytometric parameters of digitized histological sections. To our knowledge, cytometric parameters of gastritis and of adenocarcinoma have yet to be fully characterized. Our objective was to classify gastritis and adenocarcinoma based on cytometric parameters. We hypothesized that automated virtual microscopy using this novel classification can reliably diagnose gastritis and adenocarcinoma. METHODS: Routinely processed hematoxylin-and-eosin-stained histological sections from specimens that showed normal mucosa (14 cases), gastritis (35 cases), and adenocarcinoma (30 cases) diagnosed by conventional optical microscopy were scanned and digitized at high resolution. Thirty-eight cytometric parameters based on density and morphometry were applied to glands and superficial epithelium. Twelve cytometric parameters based on cytologic detail were applied to individual cells. RESULTS: Statistically significant differences in cytometric parameters for normal mucosa, gastritis, and adenocarcinoma were found. The most discriminatory parameter was the ratio of the total number of cells to the number of interstitial cells. These differences correctly classified adenocarcinoma at 100% accuracy and overall correctness was 86%. CONCLUSIONS: We describe a novel method of analyzing gastric mucosal histology based on cytometric parameters. Automated virtual microscopy can be used to classify gastric mucosa as normal, gastritis, or adenocarcinoma with reasonable accuracy. Further research is necessary to determine whether automated virtual microscopy can subclassify gastric mucosal histology in greater detail. 相似文献
994.
995.
Eva Zold Arpad Nagy Katalin Devenyi Margit Zeher Zsolt Barta 《World journal of gastroenterology : WJG》2009,15(18)
Crohn's disease (CD) is a chronic relapsing and remitting autoinflammatory disorder of the gastrointestinal tract that has many intestinal and extraintestinal complications. The purpose of treatment is long-term remission, reduction of complications, and improvement of patients' quality of life. In many cases, this can be quite challenging and it is necessary to have a well thought out management strategy. We present the case of a 38-year-old woman with fistulizing CD that manifested as diffuse abdominal pain and bloody diarrhea accompanied by arthralgia. In addition, there were ulcerative lesions surrounded by cutaneous inflammation and erythema on her extremities, indicative of pyoderma gangrenosum. The patient was treated with high doses of parenteral methylprednisolone without any improvement and was started on adalimumab. A positive response to adalimumab therapy was observed: after 2 mo of therapy, the ulcerative skin lesion healed completely and the enterogastric fistula was closed after 5 mo adalimumab treatment. Adalimumab might be a suitable initial as well as maintenance therapy in patients with complicated CD. 相似文献
996.
Increased seizure duration and slowed potassium kinetics in mice lacking aquaporin-4 water channels 总被引:9,自引:0,他引:9
The glial water channel aquaporin-4 (AQP4) has been hypothesized to modulate water and potassium fluxes associated with neuronal activity. In this study, we examined the seizure phenotype of AQP4 -/- mice using in vivo electrical stimulation and electroencephalographic (EEG) recording. AQP4 -/- mice were found to have dramatically prolonged stimulation-evoked seizures after hippocampal stimulation compared to wild-type controls (33 +/- 2 s vs. 13 +/- 2 s). In addition, AQP4 -/- mice were found to have a higher seizure threshold (167 +/- 17 microA vs. 114 +/- 10 microA). To assess a potential effect of AQP4 on potassium kinetics, we used in vivo recording with potassium-sensitive microelectrodes after direct cortical stimulation. Although there was no significant difference in baseline or peak [K(+)](o), the rise time to peak [K(+)](o) (t(1/2), 2.3 +/- 0.5 s) as well as the recovery to baseline [K(+)](o) (t(1/2), 15.6 +/- 1.5 s) were slowed in AQP4 -/- mice compared to WT mice (t(1/2), 0.5 +/- 0.1 and 6.6 +/- 0.7 s, respectively). These results implicate AQP4 in the expression and termination of seizure activity and support the hypothesis that AQP4 is coupled to potassium homeostasis in vivo. 相似文献
997.
Zsolt J. Balogh Karlijn van Wessem Osamu Yoshino Frederick A. Moore 《World journal of surgery》2009,33(6):1134-1141
Postinjury (primary) abdominal compartment syndrome (ACS) was described more than 15 years ago as severe abdominal distension
with high peak airway pressures, CO2 retention, and oliguria, which led to unplanned re-exploration after damage-control laparotomy. Later, a more elusive type
of ACS was recognized, which develops without abdominal injuries (secondary ACS). Both syndromes were recently characterized,
their independent predictors were identified, and preventive strategies were developed to reduce their incidence. Once viewed
as a syndrome with almost uniform mortality, systematic preventative strategies and therapeutic efforts have reduced the prevalence,
morbidity, and mortality of the syndrome. This review was designed to summarize the recent advances in the management of ACS,
to classify the currently available evidence, and to identify future directions of research and clinical care. 相似文献
998.
Robert Bissonnette Richard G. Langley Kim Papp Robert Matheson Darryl Toth Micki Hultquist Gregory P. Geba Barbara White 《Archives of dermatological research》2009,301(6):429-442
New biologic therapies focused primarily on cytokine pathways, some targeting T cell-mediated immune responses, are being
developed for the treatment of psoriasis. Siplizumab is a humanized anti-CD2 monoclonal antibody that interferes with costimulation
necessary for T cell activation and proliferation. We assessed the biological activity, serum concentrations, and pharmacodynamic
effects of siplizumab in patients with plaque psoriasis. Two multicenter, phase II randomized, double-blind, placebo-controlled
studies were conducted: one study randomized 124 patients to one of two intravenous (IV) doses (0.012 and 0.04 mg/kg) of siplizumab,
given every 2 weeks × 8 doses; the other study randomized 420 patients to one of three subcutaneous (SC) dose regimens of
siplizumab given weekly (5 mg for 12 weeks, 5 mg for 6 weeks, and 7 mg for 4 weeks) or placebo for 12 weeks. Adults with plaque
psoriasis involving ≥10% of the body surface area and who were not receiving psoriasis therapy were eligible. Treatment with
siplizumab resulted in reductions in psoriasis severity, but most of the effects were not statistically significant compared
with placebo. Statistically significant differences among IV siplizumab-treated and placebo groups were observed at study
day 28, with greater psoriasis area and severity index (PASI) score reductions from baseline in the siplizumab groups. The
difference in PASI50 response rates between the 0.04 mg/kg siplizumab and placebo groups was also statistically significant
at day 28. A trend toward clinical improvement was observed in SC siplizumab-treated groups. Significant reductions in circulating
absolute lymphocyte counts and CD2+ (CD3+, CD8+, and CD16+/56+), but not CD2− (CD19+ and CD14+), lymphocyte populations were observed. These changes were not accompanied by concomitant reductions in infiltrating CD3+ lymphocytes in psoriatic lesions, epidermal thickness, or keratin 16 (K16) and intercellular adhesion molecule (ICAM) expression.
The effect of siplizumab did not differentially affect CD45RO+ and CD45RA+ lymphocytes. Low or undetectable mean trough serum concentrations of siplizumab following IV or SC treatment were observed.
Pharmacokinetic data coupled with higher-than-expected placebo clinical response rates may partly explain siplizumab’s marginal
clinical activity. Higher doses of siplizumab may be required to detect significant improvements in psoriasis; however, further
development of this agent was not planned. 相似文献
999.
1000.
A technique is described for registering centric relation in dentate patients using an anterior deprogrammer to prevent muscle splinting. Properly executed, the patient is able to close into centric relation unassisted, eliminating the possibility of operator-induced error associated with commonly accepted manipulative techniques. Verification is possible visually when articulating casts by confirming the precise alignment of the mandibular incisors with occlusal markings on the deprogrammer attached to the maxillary cast. 相似文献