17β-雌二醇对肾上腺素诱导的心肌细胞肥大及原癌基因c-fos表达的影响

目的利用体外培养的乳鼠心肌细胞,观察17β雌二醇(17βestradiol)对肾上腺素(phenylephrine)诱导的心肌细胞肥大及其原癌基因cfos蛋白表达的影响。方法以培养的乳鼠心肌细胞为模型并分组给药后,用计算机图像分析软件测量心肌细胞表面积,免疫细胞化学方法检测心肌细胞原癌基因cfos的蛋白表达。结果17β雌二醇明显抑制肾上腺素诱导的心肌细胞表面积增大,同时减弱肥大心肌细胞原癌基因cfos的蛋白表达。结论17β雌二醇可抑制肾上腺素诱导的心肌细胞肥大,其作用机制可能和抑制原癌基因cfos的蛋白表达有关。     

经皮肝穿胆道内支架置入术治疗恶性胆管梗阻

目的：探讨经皮肝穿胆道内支架置入术治疗恶性胆管梗阻的疗效及技术要点。方法：３５例恶性胆管梗阻采用经皮肝穿刺肝管胆道内支架置入术，其中胰头癌１３例，肝转移癌５例、肝门淋巴结转移压迫胆管７例、胆管癌１０例。结果：共置入３种４３枚金属内支架，其中８例采用双内支架，技术操作成功率１００％。术前血清总胆红素１７０．００～８６０．０ｕｍｏＩ／Ｌ，术后３１例降至１８．２～５３．６ｕｍｏＩ／Ｌ。半年以上生存率７７．１４％（２７／３５），１年以上生存率４８．５７％（１７／３５）。本组无严重并发症，轻度并发症未作特殊处理。结论：经皮肝穿胆道内支架置入术，是不能手术的恶性胆管梗阻安全、有效的治疗方法。配合动脉插管化疗可显著提高其疗效     

左肺上叶前段与舌段的CT解剖

肺段的ＣＴ解剖在小病灶的精确定位和预后方面的重要价值，近年陆续有文献描述了部分肺段的ＣＴ解剖。肺段ＣＴ鉴别的主要依据是肺裂和肺段支气管，因左肺上叶水平裂的发生率和舌段支气管的ＣＴ显示率均低，致命名前段与舌段的鉴别发生困难。作者综合１０例肺管道铸型、１０例胸部横断面标本和１００例正常肺部ＣＴ图像的观察，提出用前段静脉的段间支（ＬＶ３ｂ）作为鉴别左肺上叶前段与上舌段的影像标志。     

影响狼疮性肾炎预后的相关因素研究

目的分析影响狼疮性肾炎预后的相关因素。方法选择2009年11月~2010年11月我院收治的180例狼疮性肾炎患者作为研究对象,统计患者的性别、年龄、血压、蛋白尿、血肌酐、血红蛋白水平,并进行回顾性分析。单因素相关性分析采用χ2检验,多因素相关性分析采用Logistic回归分析。结果 180例狼疮性肾炎患者中44例（24.4%）死亡,其中,因经济困难未能透析或间隙中断透析而死亡者13例,急性肺水肿诱导致死者3例,透析期间并发感染致死者24例,狼疮性心肌病致死者3例,狼疮性脑病致死者1例。单因素分析结果显示,职业、药物过敏史、吸烟对狼疮性肾炎预后影响最为明显,差异具有统计学意义（P〈0.05）。多因素Logistic回归分析结果显示,年龄、血压、蛋白尿、血肌酐、血红蛋白水平与狼疮性肾炎预后有关,OR值分别为0.394、0.067、0.172、4.827、0.027（P〈0.05或P〈0.01）,提示以上因素均与狼疮性肾炎的预后相关。结论狼疮性肾炎患者的预后受多个环境危险因素的影响,因此在治疗过程中应避免诱发因素,尤其是感染,提高患者预后质量。     

近红外光谱一致性检验方法快速鉴别江中健胃消食片的真伪

目的 建立快速鉴别江中健胃消食片真伪的近红外光谱一致性检验方法.方法 采集16批江中健胃消食片铝塑和粉末的近红外光谱,用OPUS分析软件进行处理,分别建立江中健胃消食片铝塑和粉末的近红外光谱一致性检验方法,用江中健胃消食片伪品的近红外光谱进行验证.结果 建立的近红外光谱一致性检验方法可以快速、准确地判断出江中健胃消食片的真伪.结论 该方法是一种简便、快速、准确的分析技术,可以用于药品快速检测车的筛查和抽样.     

2004—2011年广西宜州市病毒性肝炎疫情分析

目的了解广西壮族自治区宜州市近8年法定传染病各型病毒性肝炎的发病情况,分析其流行特征和规律,为制订防治策略和措施提供科学依据。方法收集该市2004—2011年法定传染病各型病毒性肝炎疫情资料,采用Excel软件进行数据分析。结果全市近8年报道各型病毒性肝炎患者共3 769例,年平均发现阳性患者471例,男女比例为1.82∶1,甲型肝炎(甲肝)占5.52%(208/3 769)、乙型肝炎(乙肝)占76.09%(2 868/3 769)、丙型肝炎(丙肝)占15.47%(583/3 769)、戊型肝炎(戊肝)占0.24%(9/3 769)、未分型肝炎占2.68%(101/3 769)。全市肝炎人群阳性率为74.12/10万,甲肝为4.09/10万、乙肝为56.40/10万、丙肝为11.47/10万、戊肝为0.18/10万、未分型肝炎为1.99/10万。全市各乡镇每月均有肝炎阳性患者出现。15-<45岁肝炎阳性患者占总阳性数的71.19%。农民、公职人员和学生患者排前3位,丙肝在个别乡镇有增多趋势。结论该市病毒性肝炎防治任务仍然严峻,应加强病毒性肝炎的预防。     

Human chorionic gonadotropin β induces cell motility via ERK1/2 and MMP-2 activation in human glioblastoma U87MG cells

Human chorionic gonadotropin β (hCGβ) promotes tumorigenesis in a variety of tumors including glioblastoma, breast and prostate cancer cells, etc. However, the involved mechanisms remain elusive. Distinct from the other tumors, glioblastoma is a highly invasive brain tumor; invasion causes high recurrence and mortality. Characterization of hCGβ signaling is to determine therapeutic targets to inhibit invasion and lower recurrence. Through both a stable cell line over-expressing hCGβ and hCGβ standards, we tested hCGβ signaling, migration and invasion in human glioblastoma U87MG cells. ELISA showed that hCGβ secreted into culture medium at an amount of 237.8 ± 7.8 ng/10⁷ cells in hCGβ transfected stable cells after the cells were grown for 24 h. Through Western blot and Gelatin zymography, we found that hCGβ standards phosphorylated ERK1/2 and upregulated MMP-2 expression in dose- and time-dependent manners. Meanwhile, overexpressed hCGβ phosphorylated ERK1/2, and upregulated MMP-2 expression and activity, whereas ERK1/2 blocker PD98059 (25 μM) significantly decreased both ERK1/2 and MMP-2 expression and activity. In addition, in the same conditions as the signaling test, hCGβ promoted cell migration and invasion, whereas the PD98059 diminished these effects. These findings demonstrated that hCGβ phosphorylated ERK1/2 upregulating MMP-2 expression and activity leading to cell migration and invasion, suggesting that hCGβ, ERK1/2 and MMP-2 are the potential targets to inhibit glioblastoma invasion.     

Radiosensitivity of lncrna linc00909 targeting mir-548-3p on colorectal cancer cells

Objective To investigate whether lncRNA LINC00909 affected the radiosensitivity of colorectal cancer cells by targeting miR-548-3p. Methods The expression levels of LINC00909 and miR-584-3p in colorectal cancer and adjacent tissues were detected by qRT-PCR. The colorectal cancer cells SW480 and SW620 were cultured in vitro, and transfected with si-NC, si-LINC00909, miR-NC, miR-584-3p mimics, si-LINC00909, and anti-miR-NC and si-LINC00909, and anti-miR-584-3p, respectively. The cells were irradiated with a dose of 4 Gy. The cell survival fraction and sensitization enhancement ratio (SER) were detected by clone formation assay. Cell proliferation was detected by MTT assay. Cell migration and invasion were assessed by Trans well chamber assay. The targeting relationship between LINC00909 and miR-584-3p was confirmed by dual luciferase reporter assay. The effect of interfering with the expression of LINC00909 or inhibiting the expression of miR-584-3p on the weight of the xenograft tumor after irradiation was evaluated by subcutaneous xenograft experiment in nude mice. Results The expression level of LINC00909 in colorectal cancer tissues was significantly up-regulated (P<0.05), whereas the expression level of miR-584-3p was significantly down-regulated (P<0.05). After interfering with the expression of LINC00909 or miR-584-3p overexpression, the cell survival fraction score was significantly reduced (P<0.05), the SERs were 2.017 and 1.762, and cell proliferation, migration and invasion were suppressed (all P<0.05). Dual luciferase reporter assay confirmed that LINC00909 could target and bind to miR-584-3p. After interfering with the expression of LINC00909, the weight of the transplanted tumor was significantly reduced (P<0.05), whereas the weight of the transplanted tumor was significantly increased after co-transfection with anti-miR-584-3p (P<0.05). Conclusion Interfering with the expression of LINC00909 can inhibit the proliferation, migration and invasion ability of colorectal cancer cells by up-regulating the expression of miR-548-3p, thereby enhancing the cell radiosensitivity.     

鼻咽癌IMRT海马头部、体部及尾部剂量分析

目的 探讨鼻咽癌患者IMRT计划中海马头部、体部及尾部的受量。方法 选取10例接受IMRT的鼻咽癌患者,在MRI T1加权相勾画左、右侧海马的头部、体部及尾部,提取照射剂量指标做比较。对海马头部、体部及尾部受量及受照体积比较行单因素方差分析,两两比较采用LSD法。WAIS-CR言语测试结果采用配对样本t检验。结果 左、右侧海马的平均受量分别为(1147±976)、(1011±602) cGy。左侧海马头部、体部及尾部的平均受量分别为(1739±1317)、(890±982)、(547±688) cGy (P=0.042);右侧海马头部、体部及尾部的平均受量分别为(1691±942)、(744±483)、(531±603) cGy (P=0.002)。海马头部、体部及尾部受量依次减少,不同照射剂量受照体积也依次减少。结论 鼻咽癌患者IMRT中海马头部、体部及尾部受量不同,海马头部受照量最高,值得临床关注。     

护士进修需求评估量表的编制及信度、效度检验

目的 编制适用于临床护士进修需求的评估量表,并检验其信度和效度,为护士进修需求提供评估的工具.方法 通过查阅文献和质性访谈形成量表条目池,经两轮德尔菲法专家咨询,同时调查170名进修护士进行信度、效度检验后确定量表.结果 正式量表共20个条目,因子分析提取4个公因子,累计方差贡献率65.40％,分别为培训与考核需求、被...