应用模糊数学对医学生在校成绩的综合评价及对策

本文以我院 95年级临床医学专业本科 2 71名学生在校四年共 41门课程的考试成绩 ,应用模糊数学评定方法进行了学生成绩总体水平的评价 ,总评成绩 72 .61分为中等水平。针对影响成绩的因素提出了以提高教师的教学水平 ,调动学生的学习主动性和积极性 ,建立和完善教学督导制度 ,引入竞争激励机制 ,完善试题及考试质量分析与评价制度 ,改革考试模式与要求等对策。     

乙型肝炎患者血清丁型肝炎病毒标志的研究

目的 研究ＨＤＶ重叠ＨＢＶ感染对乙型肝炎患者病情和血清学指标的影响。方法 采用ＥＬＩＳＡ法检测ＨＤＶ血清标志ＨＤＡｇ、抗ＨＤ、抗ＨＤＩｇＭ和ＨＢＶ血清标志ＨＢｓＡｇ、抗ＨＢｓ、ＨＢｅＡｇ、抗ＨＢｃ、抗ＨＢｃｌｇＭ,ＰＣＲ法检测ＨＢＶＤＮＡ。结果 ３１６例乙型病毒性肝炎中６０例重叠ＨＤＶ感染,重叠感染率为１８．９８％;ＣＨ和ＦＨ组ＨＤＶ感染率显著高于ＡＨ,ＦＨ组ＨＤＶ复制指标阳性率显著高于ＣＨ及ＡＨ     

76例老年人蛛网膜下腔出血的临床特点

目的 探讨老年人蛛网膜下腔出血的特点。方法 回顾分析１９８９年１月－１９９８年８月收治６０岁以上原发性蛛网膜下腔出血７６例的临床资料。结果 本组老年蛛网膜下腔出血的临床特点为：（１）头痛发生率低且程度较轻,脑膜刺激征不明显;（２）意识障碍,精神症状明显;（３）消化道出血,ＥＣＧ明显异常及血糖明显升高等并发症多见。结论 如老年人突然出现眩晕、精神异常或突发颈、臂、腰背疼痛等脊神经刺激征状时,应考虑到     

精氨酸对肠粘膜屏障功能的影响

目的：了解精氨酸对肠粘膜屏障功能的影响。方法：雄性ＳＤ大鼠３０只,随机分为三组。精氨酸组予精氨酸０．２５ｇ腹腔注射,假手术组和肠梗阻组予生理盐水作对照,１次／ｄ,共５ｄ。再手术将肠梗阻组、精氨酸组大鼠结扎回肠,造成单纯性机械性肠梗阻模型,假手术组仅作剖腹探查。术后２４ｈ处死大鼠采集标本。结果：①细菌移位率和移位数水平肠梗阻组和假手术组相比明显升高,精氨酸组和肠梗阻组相比明显减少。②血浆内毒素水平肠     

葛根素治疗52例冠心病心绞痛疗效观察

目的：探讨冠心病心绞痛药物治疗的效果。方法：将１０４例冠心病心绞痛患者随机分成两组：Ａ组选用葛根素注射液５００ｍｇ加入５％葡萄糖５００ｍｌ静脉滴注治疗,Ｂ组采用镁－极化液５００ｍｌ静脉滴注,１次／ｄ,１４ｄ为１个观察疗程。结果：缓解心绞痛症状,Ａ组总有效率达８４．６％,与Ｂ组总有效率４２．３％相比较有非常显著差异（Ｐ〈０．０１）心肌缺血改善后心电图评定,Ａ组总有效率６９．２３％,与Ｂ组总有效率３４     

Decreased frequency of a novel T-lymphocyte subset,CD3+CD4−CD7+CD57− T cells,in hepatitis B virus-related end-stage liver disease might contribute to disease progression

CD7 and CD57 are related to the differentiation and functional stages of CD8⁺ T cells. However, the role of their combined presence in CD8⁺ T cells in patients with chronic hepatitis B virus (HBV) infection, especially those with end-stage liver disease, remains unclear. Blood samples from healthy volunteers and patients with chronic hepatitis B were analyzed via Luminex assay and ELISA to measure plasma cytokine levels. Further, recombinant IL-22 was used to stimulate peripheral blood mononuclear cells from healthy volunteers, and the frequency of CD3⁺CD4⁻CD7⁺CD57⁻ T cells and apoptosis rates were investigated via flow cytometry. Patients with end-stage liver disease, particularly those with acute to chronic liver failure, showed decreased CD3⁺CD4⁻CD7⁺CD57⁻ T cell frequency. Furthermore, the prevalence of CD3⁺CD4⁻CD7⁺CD57⁻ T cells was negatively correlated with disease severity, prognosis, and complications (ascites). We also observed that IL-22 promoted apoptosis and brought about a decrease in the number of CD3⁺CD4⁻CD7⁺CD57⁻ T cells in a dose-dependent manner. CD3⁺CD4⁻CD7⁺CD57⁻ T cells displayed a B and T lymphocyte attenuator (BTLA)^highCD25^highCD127^high immunosuppressive phenotype and showed low interferon-γ, tumor necrosis factor-α, granzyme A, and perforin expression levels. The present findings will elucidate the pathogenesis of HBV-related end-stage liver disease and aid the identification of novel drug targets.     

Characterization and phylogenetic analysis of a neurovirulent Zika virus isolated from Cambodia in 2019

The geographic range of Zika virus (ZIKV) has expanded from Asia to the Americas, leading to the 2015–2016 pandemic with enhanced neurovirulence. At present, ZIKV is continuously circulating in many Southeast Asian countries. Unfortunately, the persistent evolution of ZIKV in Southeast Asia and its influence on the biological characteristics of the virus remain incompletely understood. In this study, the in vitro and in vivo properties of a new ZIKV isolate obtained from Cambodia in 2019 (CAM/2019) were characterized and compared with those of the Cambodian strain (CAM/2010). Compared with CAM/2010, the CAM/2019 virus showed similar plaque morphology and growth curves in cell cultures and induced comparable viremia and organ viral loads profiles in both BALB/c and A129 (IFNAR1^−/−) mice upon intraperitoneal (i.p.) inoculation. Remarkably, the CAM/2019 virus exhibited enhanced neurovirulence in neonatal mice compared with CAM/2010, with a 74-fold reduction in the 50% lethal dose (LD₅₀). Consistently, CAM/2019 produced higher viral loads in the brains of BALB/c neonatal mice than CAM/2010 did. Sequence alignment showed that the CAM/2019 virus has acquired 12 amino acid substitutions, several of which were found to be associated with neurovirulence. In particular, the CAM/2019 virus shared an A1204T substitution in NS2A with the Thai isolate SI-BKK02 that was isolated from a microcephaly case. Taken together, our results indicate that a ZIKV strain isolated with specific mutations has emerged in Cambodia, highlighting the need for extensive molecular and disease surveillance in Cambodia and other Asian countries.     

五灵胶囊治疗慢性肝炎的临床随访观察

0　引言　五灵丸治疗慢性病毒性肝炎取得了较好的疗效 [1 ] ,但五灵丸为大蜜丸 ,不易被患者所接受 ,且其消化道副作用较明显 .为此 ,我们改进剂型 ,研制成五灵胶囊 ,为明确其能否保持远期疗效 ,我们对用该药治疗的患者进行随访观察 ,同时与五灵丸的疗效进行比较 .1　对象和方法1.1　病例选择　按慢性肝炎的诊断标准选择住院患者 10 0例 ,随机分为两组 .治疗组为五灵胶囊组 ,对照组为五灵丸组 .每组各 5 0例 .治疗组轻度 19例 ,中度 2 0例 ,重度 11例 .对照组轻度 2 0例 ,中度 18例 ,重度 12例 .两组均选择 18～5 8岁 ,病程 1～ 2 0 a.平均 4…     

大鼠急性颅脑损伤后脑微循环的变化

目的 研究鼠急性颅脑损伤后脑微循环的改变,以找出影响颅脑损伤与恢复变化的规律。方法 大鼠１００只随机分为５组,采用自由落体致大鼠脑损伤,伤后０．５,３．２４和１６８ｈ取材,研究脑微血管形态学变化,并用核磁共振波谱法分析颅脑损伤局部脑组织代谢的变化。结果 大鼠实验性脑损伤后０．５～２４ｈ大脑皮层微血管减少,并有“微无血管区”,微血管内可见微血栓形成;血脑屏障（ＢＢＢ）通透性增加,出现脑水肿,颅脑损伤