Gingival crevicular fluid levels of SLIT3 are increased in periodontal disease

This study aims to investigate the levels of SLIT3 in gingival crevicular fluid (GCF) of healthy and periodontal disease subjects, and their correlations to periodontal disease. A total of 45 periodontal patients and 45 periodontally healthy volunteers were enrolled. The clinical parameters, radiographic bone loss and the levels of SLIT3, receptor activator of NF‐κB ligand (RANKL) and osteoprotegerin (OPG) in GCF were measured. The prevalences of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia in subgingival plaque were also analyzed. The expression of SLIT3 and RANKL was detected in the periodontium of experimental periodontitis in rats and lipopolysaccharide (LPS)‐induced mouse macrophage. The total amounts and concentrations of SLIT3 and RANKL were significantly higher in periodontitis than those in healthy, while the level of OPG was significantly lower (p < .05). Significant positive correlations were observed between the level of GCF SLIT3 and clinical attachment level and radiographic bone loss (p < .05). There existed a significant positive correlation between SLIT3 and RANKL (p < .05). Increased expression of SLIT3 and RANKL was observed in the periodontium of periodontal rats. SLIT3 expression was induced by LPS stimulation in macrophages. These results suggest that SLIT3 may act as a diagnostic indicator of periodontal disease and should be further investigated.     

A facile method for the preparation of black TiO2 by Al reduction of TiO2 and their visible light photocatalytic activity

Black TiO₂ has attracted widespread attention due to its visible light absorption and wide range of applications. However, the currently reported preparation methods for black TiO₂ are not suitable for large-scale production due to its being prepared under high vacuum and over a long time. We have successfully prepared black TiO₂ under normal pressure and short time conditions. The as-prepared black titanium dioxide was characterized by XRD, XPS, TEM, UV-visible absorption spectrum and other characterization methods. The result shows that the as prepared black titanium dioxide had a disordered structure and oxygen vacancy defects on the surface, and exhibits excellent visible and near infrared absorption performance. The black TiO₂ sample was prepared under 650 °C 60 min exhibits excellent visible light photocatalytic performance, and can degrade 56% MO after visible light irradiation for 120 min.

Black TiO₂ has attracted widespread attention due to its visible light absorption and wide range of applications.     

妊娠期口腔保健对妊娠期牙龈炎的临床防治效果

目的:探讨妊娠期口腔保健对妊娠期牙龈炎的临床防治效果。方法:选取某院产检中心2018年1~12月的160例妊娠期妇女,随机纳入常规组与保健组,各80例。常规组予以口腔疾病知识宣教,保健组加强口腔保健干预,对比两组妊娠期妇女的牙龈炎发病率以及妊娠期牙龈炎患者干预前后的牙周临床指标与总体疗效。结果:常规组的牙龈炎发病率为18.8%,保健组为7.5%,保健组低于常规组(P<0.05);保健组患者干预后的PLI、BOP、PD、AL均低于常规组(P<0.05);常规组的总有效率为66.7%,保健组为83.3%,保健组高于常规组(P<0.05)。结论:对妊娠期妇女实施口腔保健干预,可降低妊娠期牙龈炎的发病率,改善牙龈炎患者的牙周临床指标,提高总体疗效,值得推行。     

细菌基因工程药物研究进展

采用肠道益生菌重构肠道生态平衡,治疗各种代谢相关慢性疾病,是当前保健和医疗行业的热门研究领域。由于人群个体差异,益生菌的治疗效果并不稳定。采用合成生物学手段,将具有特殊治疗作用的功能片段导入益生菌中,创造具有靶向治疗效果的细菌活体药物,对医药发展具有重要影响。本文阐述以各种细菌为治疗载体的活体药物研究进展,包括用于疾病治疗的细菌种类优势、封存技术以及所携带效应物的表达调控。     

原代大鼠雪旺细胞在聚甲基丙烯酸甲酯电纺纳米纤维支架上的生长

目的 探讨原代大鼠雪旺细胞(SCs)在不同拓扑结构的聚甲基丙烯酸甲酯(PMMA)电纺纳米纤维上生长的特点,为后续在体研究提供基础.方法 构建具有随机或有序拓扑结构的PMMA电纺纳米纤维,并测定其生物相容性;利用PMMA薄膜组作为对照,利用慢病毒载体转染绿色荧光蛋白基因作为显色手段,分析SCs在随机及有序纤维支架上的细胞形态和生长方向,以及胞体及突起与纤维结构的依存关系. 结果 构建的PMMA电纺纳米纤维具有良好的生物相容性,SCs在随机及有序电纺纤维上均能顺利贴壁并生长;电纺纤维的拓扑结构能够影响SCs的细胞形态,与在PMMA薄膜及随机电纺纤维上相比,在有序纤维系统上的SCs能够形成更长且与纤维延伸方向一致的细胞突起(P＜0.01).相比随机电纺纤维,有序纳米纤维对原代SCs的生长更具引导作用. 结论 PMMA有序电纺纳米纤维具有作为神经损伤后植入性SCs支架的潜力.     

口蹄疫病毒样颗粒研究进展

病毒样颗粒(VLP)具有与真实病毒粒子大小相似的结构,能引起免疫应答但不具有感染性。口蹄疫病毒是无囊膜的RNA病毒,其结构蛋白可组装成VLP,且具有良好的免疫原性。本文对口蹄疫病毒VLP的免疫机制、表达系统及应用、口蹄疫病毒VLP作为抗原在诊断和疫苗中的应用进行了综述。