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81.
Reactivity of human sera in a sensitive, high-throughput pseudovirus-based papillomavirus neutralization assay for HPV16 and HPV18 总被引:20,自引:0,他引:20
Pastrana DV Buck CB Pang YY Thompson CD Castle PE FitzGerald PC Krüger Kjaer S Lowy DR Schiller JT 《Virology》2004,321(2):205-216
Sensitive high-throughput neutralization assays, based upon pseudoviruses carrying a secreted alkaline phosphatase (SEAP) reporter gene, were developed and validated for human papillomavirus (HPV)16, HPV18, and bovine papillomavirus 1 (BPV1). SEAP pseudoviruses were produced by transient transfection of codon-modified papillomavirus structural genes into an SV40 T antigen expressing line derived from 293 cells, yielding sufficient pseudovirus from one flask for thousands of titrations. In a 96-well plate format, in this initial characterization, the assay was reproducible and appears to be as sensitive as, but more specific than, a standard papillomavirus-like particle (VLP)-based enzyme-linked immunosorbent assay (ELISA). The neutralization assay detected type-specific HPV16 or HPV18 neutralizing antibodies (titers of 160-10240) in sera of the majority of a group of women infected with the corresponding HPV type, but not in virgin women. Sera from HPV16 VLP vaccinees had high anti-HPV16 neutralizing titers (mean: 45000; range: 5120-163840), but no anti-HPV18 neutralizing activity. The SEAP pseudovirus-based neutralization assay should be a practical method for quantifying potentially protective antibody responses in HPV natural history and prophylactic vaccine studies. 相似文献
82.
Efficacy of intracytoplasmic sperm injection using intentionally cryopreserved epididymal spermatozoa 总被引:1,自引:6,他引:1
Oates Robert D.; Lobel Susan M.; Harris Doria H.; Pang Samuel; Burgess Colleen M.; Carson Ronald S. 《Human reproduction (Oxford, England)》1996,11(1):133-138
Microsurgical epididymal sperm aspiration was a great advancein the therapy of patients with non-recon-structable, obstructiveazoospermia, most notably congenital bilateral absence of thevas deferens. Using conventional in-vitro fertilization, pregnancieswere rarely achieved because the rate of oocyte fertilizationwas extremely poor. However, the use of retrieved spermatozoain conjunction with intracytoplasmic sperm injection (ICSI)has dramatically increased the likelihood of embryo formation.Typically, sperm and oocyte harvesting are performed simultaneously.We have investigated whether frozen-thawed spermatozoa workas well as fresh spermatozoa. When we had concluded from ourown population of patients (groups I and II) that they did,we adopted a policy of aspirating spermatozoa, primarily cryopreservingthem and using them for ICSI at a later date. We found the fertilizationrates of this latter cohort of patients (group III) to be excellent(37% per oocyte), and the ongoing pregnancy rate is quite satisfactory(40 % per couple, 29% per cycle). We offer this approach asan alternative to the traditional scheme because it markedlyeases the burden of partner scheduling on both the couple andthe clinicians involved. In addition, assurance of the availabilityof male partner spermatozoa can be attained prior to beginningovulation induction. 相似文献
83.
Objective. To study the redistribution ofendothelin- 1 (ET- 1 ) receptors in two subcellular organelles, the sarcolemreal membrane and the light vesicle, of rat heart during the progression of sepsis. Methods. Sepsis was induced by cecal ligation and puncture (CLP). ET1 receptor was assayed by using[125I]-ET1 binding. Marker enzyme activities, protein yield, and dry-to-wet weight ratio of cardiac membraneswere measured. Results. Septic rat heart exhibited two distinct phases: an initial hyperdynamic phase( 9h after CLP; early stage of sepsis) followed by a hypodynamic ( 18h after CLP, late stage of sepsis) phase. [125I]-ET1 binding study showed that during early stage of sepsis, the Bmax of ET1 receptors was increased by 30% in sarcolemma but decreased by 19% in light vesicles, while during late stage of sepsis, the Bmax was decreased by 24% in sarcolem-ma but increased by 38% in light vesicles. The total binding of sarcolemma and light vesicles was increased by 25% during early stage of sepsis but decreased by 17% during late stage of sepsis. Conclusions. These data indicated that ET1 receptors in the rat heart were externalized from light vesicles to sarcolemmal membranes during early hyperdynamic phase while internalized from surface membranes to intracellu-lar compartment during late hypodynamic phase of sepsis. 相似文献
84.
85.
Changes in systemic and splanchnic hemodynamics after orthotopic liver transplantation in cirrhotic rats 总被引:3,自引:1,他引:2
Previousstudieshaveshownthatsystemicandsplanchnichemodynamicabnormalitiespersistincirrhoticpatientswithportalhypertensionafterorthotopiclivertransplantation(OLT),despitethereturnofportalpressuretonormalTheelucidationofthemechanismremainscontroversial15… 相似文献
86.
碱性成纤维细胞生长因子对庆大霉素肾毒性治疗作用的 … 总被引:3,自引:1,他引:2
目的:初步观察碱性成纤维细胞生长因子(bFGF)对庆大霉素(Gentamicin,GM)肾毒性的治疗作用。方法:雄性Wistar大鼠32只,随机分为对照组、实验组(GM)中的GM1组、GM2组、GM3组各8只。GM组按160mg/kg.d^-1肌注GM8d,对照组注身等量生理盐水。于实验第9天宰杀对照组和GM1组的各8只动物,分别取血做血清尿素氮(BUN)和血清肌酐(Scr)检查,以取肾皮质做光镜 相似文献
87.
电话传送心电图监测与常规心电图诊断心肌缺血的应用比较 总被引:7,自引:0,他引:7
目的:探讨电话传送心电图检测(Transtelepomic Electrocardiographic Monitoring,TTM)心肌缺血的诊断价值。方法:同步检测97例心肌缺血患者的常规心电图(ECG)、电话传送心电图(TTM),调整后者的监测电极与ECG的电极部位完全相同,判断二者的S-T、T缺血变化。结果:ECG发现97例典型S-T、T心肌缺血患者,TTM有90例相同变化(敏感性92.5% 相似文献
88.
目的:通过对壮族人和汉族人原发性高血压与左室心肌肥厚关系的研究,从侧面探讨两个民族的原发性高血压的等位基因是否相同,从而指导临床的治疗用药.方法:壮族组30例,汉族组40例,两组患者均为原发性高血压病人.采用彩色多谱勒超声仪进行心脏检查,测量相关径线并进行比较.结果:两组患者的心超测值及左室心肌肥厚情况无显著性差异.结论:壮族人与汉族人原发性高血压左室心肌肥厚情况无差异,说明两者的等位基因有可能相同. 相似文献
89.
目的探讨二尖瓣脱垂(MVP)与急性风湿性心脏炎(ARC)的相关性。方法对108例风湿热并ARC的患者行超声心动图检查以观察MVP的发生率。结果超声心动图检查发现MVP76例,发生率为70%,其中前叶脱垂57例(53%),后叶脱垂13例(12%),前后叶均脱垂6例(6%)。与对照组比较有明显的差异(P<0.01)。ARC所致MVP中74例(97%)并发二尖辫关闭不全(MI),且MVP与MI的严重程度密切相关(P<0.01)。结论ARC是MVP,尤其是二尖瓣前叶脱垂的重要原因之一,而MVP是ARC所致MI的重要因素。 相似文献
90.
目的研究GDM对母婴的影响。方法对112例GDM孕妇和112例健康孕妇的妊娠结局进行分析。采用11配对病例—对照研究,配对的条件是孕妇年龄相近(±2),同年同月同医院分娩。结果GDM孕妇手术分娩率、早产率、妊娠高血压综合征和巨大儿发生率都较对照组高。GDM的及早诊断和及时治疗可使巨大儿发生率降低。与新生儿病率有关的因素是GDM的严重程度、血糖控制情况及巨大儿。结论早期诊断GDM及控制血糖是减少妇婴并发症的关键。 相似文献