女性飞行学员高空飞行生理生化耐受能力研究

为从高空缺氧耐力训练前后生理、生化角度探讨中国女飞行员驾驶战斗机的可行性,在地面模拟高空缺氧及高空飞行训练前后行心电图、心率、血氧饱和度、血压、血尿酸(BUA)、肌酸激酶(CK)、血红蛋白(Hb)、乳酸(BLA)、肌酐(Cr)和天冬氨酸转氨酶(AST)测定,并与男飞行学员的测定结果比较.发现男女飞行学员的心电图、心率、血氧饱和度、血压、Cr、CK、BUA和AST均无显著性差异;女飞行学员在高空耐缺氧Hb代偿反应高于男飞行学员(P<0.05),而BLA水平低于男飞行学员(P<0.05).认为中国女飞行学员在生理生化耐受能力上能胜任飞战斗机.     

A simple method for coating native polysaccharides onto nitrocellulose.

A method for coating native, non-derivatized, polysaccharide (PS) onto nitrocellulose (NC) for identifying PS-specific antibodies has been developed. The new feature of this method is that PS molecules are vacuum filtered onto NC in their native state by devices that can accommodate NC of different sizes and shapes. PS-coated NC disks were used to localize antibody secreting hybridoma cells cultured on filter paper disks. These were analyzed by blotting with size-matched PS-coated NC disks and specific antibodies secreted by individual colonies were detected by enzyme-linked immunoblot. In another application of this method, immune sera were separated by isoelectric focusing and the gels were blotted with PS-coated NC sheets. The spectrotype and isotype of antibodies that bound to the NC were examined using isotype specific enzyme-linked antibody. These immunoblots showed high resolution and specificity. The advantages of this method are that the PS used for coating does not need to be derivatized in order to bind the NC, and that smaller quantities of PS may be utilized by this coating method when compared to other techniques. This provides a useful tool to ask many questions regarding the immune response to PS.     

带血管蒂前臂后皮神经电缆式移植修复上臂桡神经缺损

目的：介绍带血管蒂前臂后皮神经电缆式移植修复上臂桡神经缺损的应用解剖、手术方法及临床效果。方法：在２０侧成人上肢标本上，观测前臂后皮神经及桡侧副动脉的行程及外径，设计以桡侧副动脉为蒂的前臂后皮神经电缆式移植修复上臂桡神经缺损的新术式并应用于临床。结果：前臂后皮神经与桡侧副动脉伴行关系密切，具有形成带血管蒂前臂后皮神经转移的解剖学条件；前臂后皮神经横径２．０ｃｍ左右，可切取长度１５．０ｃｍ左右，折叠后可以修复７．０ｃｍ左右的桡神经缺损。同时，术中发现神经供体断端出血活跃，说明带血供的前臂后皮神经移植的血供是可靠的。临床应用８例，神经缺损３．５～６．０ｃｍ，随访１０个月～３年，效果满意。按陆裕朴介绍的评定标准：优４例，良３例，可１例。结论：该术式转移灵活，操作简单、安全、可靠，对供区影响小，是修复上臂桡神经缺损的一种有用的方法。     

中药饮片加工炮炙在临床中的意义

本文简述了中药炮炙的根据、原理、方法以及各种不同辅料和炮炙方法对饮片药性与作用的影响。说明不同的炮炙方法可以直接影响和改变中药的药性和作用,影响到临床疗效,因此,必须重视中药饮片的炮炙工作。以确保临床安全有效的用药。     

梗阻性黄疸患者的内毒素血症及其对机体的影响

４９例梗阻性黄疸患者（实验组）和８１例无黄疸的胆系疾病患者（对照组）围手术期外周血的内毒素（ＥＴ）检测结果表明，实验组：①术前ＥＴ阳性率明显高于对照组（Ｐ＜０．００１）；②术后１０～１２ｄ夹闭Ｔ／Ｙ管后ＥＴ阳性率明显低于术前和夹管前（Ｐ＜０．００１）；③伴急性化脓性胆管炎（ＡＣＳＴ）的患者ＥＴ阳性率明显高于无ＡＣＳＴ的患者（Ｐ＜０．００１）；术前口服胆盐的患者ＥＴ阳性率则显著低于未口服胆盐者（Ｐ＜０．００１）．作者认为，本组梗阻性黄疸患者中出现的肾功能障碍、胃肠道出血以及２例患者的死亡，至少部分地与内毒素血症有关。     

Distinguishment of A 42 Capsomere Structure of Icosohedral Virion from A 32 One by Electron Microsco

ＤｉｓｔｉｎｇｕｉｓｈｍｅｎｔｏｆＡ４２ＣａｐｓｏｍｅｒｅＳｔｒｕｃｔｕｒｅｏｆＩｃｏｓｏｈｅｄｒａｌＶｉｒｉｏｎｆｒｏｍＡ３２ＯｎｅｂｙＥｌｅｃｔｒｏｎＭｉｃｒｏｓｃｏｐｅＣｎｅｎＢｉｎｇｙｉｎｇ（陈丙莺）；ＺｈｏｕＴｏｎｇ（周）（Ｄｅｐａｒｔｍｅ...     

EXPERIMENTAL STUDY OF RADIOIMMUOGUIDED SURGERY IN OVARIAN CARCINOMA

INTRODUCTIONThemaintreatmenttodateforovariancarcinomaissurgicalresection,thesocalledcytoreductivesurgery.Toperformacompleteresection,surgeonsusuallydependonvisualandtactilesensesaswellassurgicalinstinctswhicharerelativelycrudeinidentifyingsubclinicalmicroscopictumor.Radioimmuno--guidedsurgerysystem(RIGS)hasbeentakenoverthelast10yearsinbothlaboratoryandclinicalsettings.However,onlyafewreportswereseenaboutitsstudyinovariancancerworldwideespeciallyinourcountry.OurstudyincludeestablishingaR…     

B超,CT与DSA对肝癌介入治疗的指导价值

对１２６列原发性肝癌与３７例转移性肝癌介入治疗前均进行了Ｂ超、ＣＴ与数字减影血管造影（ＤＳＡ）检查。ＤＳＡ显示原发性肝癌多数血供丰富，而转移性肝癌以少血供者为主；ＣＴ显示小病灶的敏感性稍差，病灶的密度和均匀性与血供多少有一定关系，但特异性不高，而病灶的增强次应与血供关系密切，多血供者中９５％有显示的增强效应，少血供者仅占４．７％；Ｂ超所示病灶的回声高低与血供亦有一定关系，但特异性稍差。结果表明，Ｄ     

Adipose tissues differentiated by adipose-derived stem cells harvested from transgenic mice

Objective: To induce adipocyte differentiation in vitro by adipose-derived stromal cells (ASCs) harvested from transgenic mice with green fluorescent protein (GFP) and assess the possibility of constructing adipose tissues via attachment of ASCs to typeⅡcollagen scaffolds. Methods: Inguinal fat pads from GFP transgenic mice were digested by enzymes for isolation of ASCs (primary culture). After expansion to three passages of ASCs, the cells were incubated in an adipogenic medium for two weeks, and the adipocyte differentiation by ASCs in vitro was assessed by morphological observation and Oil Red O staining. Then they were attached to collagen scaffolds and co-cultured for 12 hours, followed by hypodermic implantation to the dorsal skin of nude mice for 2 months. The newly-formed tissues were detected by HE staining. Results: The cultured primary stem cells were fibroblast-like and showed active proliferation. After being incubated in an adipocyte differentiation medium, the lipid droplets in the cytoplasm accumulated gradually and finally developed into mature adipocytes, which showed positive in Oil Red O staining. A 0. 5-cm3 new tissue clot was found under the dorsal skin of the nude mice and it was confirmed as mature adipose tissues by fluorescent observation and HE staining. Conclusions: ASCs can successfully differentiate adipose tissues into mature adipocytes, which exhibit an adipocyte-like morphology and express as intracytoplasmic lipid droplets. It is an efficient model of adipose tissues engineered with ASCs and type I collagen scaffolds.     

疏可眠胶囊镇静作用的实验研究

且的:观察疏可眠胶囊对小鼠的镇静作用。方法:采用旷野活动法观察疏可眠胶囊不同给药剂量、给药时间对小鼠自主活动的量效、时效作用;对士的宁诱发惊厥小鼠的作用。结果:连续给药7d,疏可眠低中剂量(1g·k~(-1)、2g·kg~(-1))均能显著减少小鼠的自主活动;疏可眠高剂量(3g·kg~(-1))给药后1h～2h自主活动显著减少;给药后2h,呈现量效关系,随着剂量增加小鼠自主活动减少。各剂量组均可延长士的宁诱发小鼠惊厥出现的时间及死亡时间。结论:疏可眠胶囊具有减少小鼠自主活动和抗惊厥作用。