Cell transplantation to improve ventricular function in the failing heart

Current therapies for congestive heart failure are limited in efficacy or in applicability. Cardiac cell transplantation offers a novel therapeutic approach to improve heart function. Although significant progress has been made over the past decade in the development of cell transplantation, only recently have investigators studied the changes in ventricular function following cell transplantation. This review article describes the latest research developments, evaluates recent studies of ventricular function after cell transplantation, and discusses the future directions of cell transplantation as a new therapy to ‘repair broken hearts’.     

瘢痕内切除术在治疗瘢痕疙瘩中的临床应用

目的：探讨一种较为经济有效地综合治疗瘢痕疙瘩的临床途径，方法：在瘢痕内切除大部分瘢痕组织，将保留的创缘两侧瘢痕皮肤修薄，无张力缝合，拆线后给予曲安缩松注射，一般3-5次，共治疗220例。结果：213例痊愈，随访6个月未复发，7例因月经紊乱等原因而放弃治疗，导致部分复发。结论：该方法简单、经济，不产生新的供皮区，给予曲安缩松注射次数少，较传统方法留瘢痕小。     

指数曲线电刺激对周围神经移植的组织学影响

目的 :研究指数曲线电刺激对周围神经移植修复后神经再生的影响。方法 :将 1 2 0只Wistar成年雄性大鼠制成坐骨神经移植模型 ,随机分为A组 (对照组 )、B组 (弥可保组 )、C组(指数曲线电刺激组 ) ,给予不同的治疗 ,在不同时段观察步态、毛发、展爪反射 ,测定SFI、神经传导速度、小腿三头肌湿重等指标。结果 :指数曲线电刺激有助于神经传导的恢复 ,减慢小腿三头肌失神经的废用 ,加快SFI及神经远段变性的恢复。结论 :指数曲线电刺激有利于神经再生。     

RANK signaling is not required for TNFalpha-mediated increase in CD11(hi) osteoclast precursors but is essential for mature osteoclast formation in TNFalpha-mediated inflammatory arthritis.

To address the controversy of whether TNFalpha can compensate for RANKL in osteoclastogenesis in vivo, we used a TNFalpha-induced animal model of inflammatory arthritis and blocked RANKL/RANK signaling. TNFalpha increased osteoclast precursors available for RANK-dependent osteoclastogenesis. RANK signaling is not required for the TNFalpha-stimulated increase in CD11b(hi) osteoclast precursors but is essential for mature osteoclast formation. INTRODUCTION: Although critical roles of TNFalpha in inflammatory arthritis and RANKL in bone resorption have been firmly established, a central controversy remains about the extent to which TNFalpha can compensate for RANKL during osteoclastogenesis and the stage at which RANK signaling is required for osteoclastogenesis. Here, we used the human TNFalpha transgenic mouse model (TNF-Tg) of erosive arthritis to determine if there are both RANK-dependent and -independent stages of osteoclastogenesis in TNFalpha-induced erosive arthritis. MATERIALS AND METHODS: Osteoclastogenesis and osteoclast precursor (OCP) frequency were analyzed using histology, fluorescence-activated cell sorting (FACS), and cell culture from (1) TNF-Tg mice treated with the RANKL antagonist, RANK:Fc, or (2) TNF-Tg X RANK -/- mice generated by crossing TNF-Tg mice with RANK-/- mice. RESULTS: Treatment of TNF-Tg mice, which have increased OCPs in their spleens, with RANK:Fc dramatically reduced osteoclast numbers on the surface of their arthritic joints and within their bones, but did not decrease CD11b(hi) OCP numbers in their spleens. Long-term RANK:Fc administration alleviated joint erosion. Furthermore, TNF-Tg x RANK -/- mice had severe osteopetrosis, no osteoclasts, and no joint erosion, but increased CD11b(hi) precursor numbers that failed to form mature osteoclasts in vitro. CONCLUSION: RANK signaling is essential for mature osteoclast formation in TNFalpha-mediated inflammatory arthritis but not for the TNFalpha-induced increase in CD11b(hi) OCP that subsequently can differentiate into osteoclasts in inflamed joints.     

宫腔镜辅助下分段诊刮术与单纯分段诊刮术诊断子宫内膜癌的比较

为比较宫腔镜辅助下分段诊刮术与单纯分段诊刮术在诊断子宫内膜癌中的临床意义 ,将经手术后病理证实的子宫内膜癌患者分为 2组 :宫腔镜辅助下分段诊刮组 (A组 ) 3 1例 ;单纯分段诊刮组 (B组 ) 3 9例。比较 2组术前诊断子宫内膜癌的准确性及开腹手术时腹水细胞学的检查结果。 2组患者的年龄、临床病理分期、病理分级及组织学类型差异无显著性。A组诊断宫颈受累的准确率为 96.77% (3 0 / 3 1 ) ,假阳性率为 3 .2 3 % (1 / 3 1 ) ;B组分别为 79.49%(3 1 / 3 9) ,1 5 .3 8% (6/ 3 9)。 2组准确率比较 ,差异有显著性 (P <0 .0 5 )。取腹水或腹腔冲洗液行细胞学检查。A组细胞学阳性率为 6.45 % (2 / 3 1 ) ,B组为 1 4.2 9% (3 / 2 1 ) ,2组间差异无显著性 (P >0 .0 5 )。提示 :宫腔镜辅助下分段诊刮术可提高术前诊断子宫内膜癌的准确性 ,从而避免不必要地扩大手术范围 ,而且不增加腹腔内播散的危险。     

大鼠慢性应激性水浸溃疡模型的复制

本文复制了慢性、应激性水浸溃疡模型,23℃每天拘束水浸4小时,4天后所得溃疡病变明显,溃疡指数是13.8士4.5mm~2,且再现性良好,是一个较好的慢性、应激性溃疡模型。哌哌西平对此溃疡具有较强的防治作用。     

脉冲振荡技术在儿童支气管舒张试验中的应用

目的：探讨脉冲振荡方法(IOS)在儿童支气管舒张试验中的应用及其与常规肺量计法结果的相关关系。方法：发作期的哮喘患儿同时应用两种方法做吸入支气管扩张剂前后肺功能测定，以FEV1改善≥15％判断为阳性，应用统计学处理对两种方法各指标作相关性分析。结果：IOS各指标舒张后改善明显，△Zrs、△R5与金标准△FEV1密切相关，经多元回归得出方程△FEV1=-1．527△Zrs 0．883△R5。结论：IOS法可以作为不能配合用力呼气动作的儿童行支气管舒张试验。     

The characteristics of Hep-2 cell with multiple drug resistance induced by Taxol.

OBJECTIVE: To investigate the characteristics of Hep-2 cell with multidrug resistance (MDR) induced by Taxol. STUDY DESIGN: Hep-2 cells were exposed in stepwise escalating concentration of Taxol to develop the resistant cell line-Hep-2T. Cell cycle distribution, apoptosis, and rhodamine accumulation were studied through flow cytometry. The MDR1 and MRP1 genes were detected through real-time quantitative RT-PCR, and the corresponding proteins were detected through Western blotting. RESULTS: The drug resistance of Hep-2T cells to Taxol, doxorubicin, gemcitabine, 5-FU, and cisplatin all increased. The percentage of G0/G1 phase and the antiapoptosis ability increased significantly compared with Hep-2 cells. Both MDR1 and MRP1 also increased at gene and protein level, though MDR1 was more prominent. CONCLUSION: More emphasis should be laid on MDR1/Pgp, the non-Pgp substrate chemotherapeutic agents, and the changes of cell cycle distribution to prevent MDR induced by Taxol. SIGNIFICANCE: These findings may provide theoretical support for the reverse of MDR.     

葛根素对抗H2O2引起血管平滑肌细胞凋亡及坏死

目的：研究葛根素对过氧化氢引起无血清培养的小牛主动脉血管平滑肌细胞凋亡和坏死的影响。方法：通过体外细胞培养，以噻唑兰（MTT）法测定细胞存活率，用流式细胞术检测细胞DNA含量及凋亡细胞百分率；DNA琼脂糖凝胶电泳法观察细胞凋亡过程中DNA断裂程度。结果：H2O2诱导无血清培养的平滑肌细胞凋亡，葛根素可显著降低平滑肌细胞中凋亡细胞百分率，并减少凋亡细胞DNA断裂，同时也减少其引起的平滑肌细胞坏死。结论：葛根素可对抗H2O2引起的无血清培养的血管平滑肌细胞凋凋亡及坏死。     

3D-CTA在颅内动脉瘤诊治中的应用

目的探讨3D-CTA在颅内动脉瘤诊断和治疗中的应用价值。方法采用3D-CTA检查疑似颅内动脉瘤病人52例,根据造影结果决定直接手术或进一步行3D-DSA检查。术后均复查3D-CTA。结果共发现58个动脉瘤,6例查3D-CTA及3D-DSA均未发现动脉瘤。3D-CTA对颅内动脉瘤诊断的敏感度、特异度、阴性似然比分别是94.8%、100%、0.052。诊断颅内动脉瘤病人的敏感度、特异度、阴性似然比分别是97.8%、100%、0.022。术前3D-CTA在估计动脉瘤大小及形态方面与术中所见 3D-CTA结果相似。3D-CTA清晰显示了动脉瘤的自身形态和周边的关系。结论3D-CTA是诊治颅内动脉瘤的一种快捷、安全、操作简便的方法,为制定手术方案提供了详细的资料,具有很高的临床实用价值。