Enterovenous fistula: unusual complication of Crohn disease

An unusual complication in a patient with Crohn enterocolitis is presented. Ultrasound and computed tomographic studies showed stationary gas in the portal vein, and a presumptive diagnosis of septic ascending portal thrombophlebitis was made. Emergency laparotomy was performed and the terminal ileum was excised for recurrent Crohn disease. Pathologic examination showed a fistula from the lumen of the inflamed segment to the superior mesenteric venous system. Autopsy 1 month later showed organizing thrombi and fecal debris in the portal venules.     

β-淀粉样肽鼠脑室内灌注制作阿尔茨海默病动物模型的实验技术研究

目的　探讨 β -淀粉样肽鼠脑室内灌注制作阿尔茨海默病 (AD)模型过程中的技术问题。 方法　经颅骨穿刺进入鼠侧脑室 ,以微渗透泵持续灌注 β -淀粉样肽 (Aβ) ,使Aβ弥散入脑室周围及皮层 ,造成与神经元相关的Aβ沉积等类似AD的病理变化。 4周后鼠脑冠状位连续切片 ,在普通光学显微镜下观察脑导管进入侧脑室的痕迹。结果　大鼠有 92 % (5 5 /6 0 )导管准确进入侧脑室 ,接近进入或在皮层 5 % (3/6 0 ) ,小鼠仅 4 3% (2 3/5 4 )导管进入侧脑室 ,接近进入或在皮层 31% (17/5 4 )。结论　大鼠脑室内灌注Aβ的方法可作为AD动物模型的方法之一。     

Guidelines for blood utilization review

Hospitals are required by accrediting agencies to perform blood utilization review. Specific areas that must be addressed are the ordering, distribution, handling, dispensing, and administration of blood components. Monitoring the effects of transfusion on patients is also required. The format of the review process and the criteria for appropriate blood utilization must be developed by each institution. This article provides examples of areas that can be reviewed and procedures that may be used. However, the suggested laboratory values must not be interpreted as defining indications or criteria for transfusion. Each transfusion committee, or its equivalent, is responsible for developing its own institutional blood utilization procedures and audit criteria. Review and approval by the medical staff prior to implementation are essential. The procedures must also be reviewed and revised on a regular basis.     

大鼠原位肝移植模型建立方法的若干问题探讨

目的探讨建立大鼠原位肝移植模型的方法与技术。方法用改进的二袖套法行100例Lewis→BN大鼠原位肝移植,术中门静脉、肝下下腔静脉用袖套法吻合,肝上下腔静脉用缝合法吻合,胆管内支架法完成胆道重建。结果供体手术时间(30.9±5.0)min,供肝修整时间(10.0±3.0)min,受体手术时间(50.0±5.5)min,无肝期为(23.0±2.5)min。手术成功率为92%(术后存活2 d以上),1周存活率为87%,1个月存活率为84%。结论通过提高手术技巧,熟悉各种并发症的原因和预防处理措施,可以减少并发症的发生,提高手术成功率,延长大鼠术后存活时间。     

浙江省实验动物中心长爪沙鼠群体遗传状况分析

目的 探讨浙江实验动物中心长爪沙鼠群体的遗传状况。方法 应用生化基因位点遗传检测方法，测定初始和生物净化长爪沙鼠群体27个生化位点等位基因的分布。结果 2个长爪沙鼠群体均具有丰富的遗传多样性；生物净化群体与初始群体比较有17．86％的基因丢失率，但尚未出现明显的遗传分化现象（FST〈0．05）。结论 群体均偏离了哈迪-温伯格平衡（P〈0．05）。     

Are chest radiographs and electrocardiograms still valuable in evaluating new pediatric patients with heart murmurs or chest pain?

OBJECTIVES: To determine the usefulness of electrocardiography (ECG) and chest radiography (CXR) in evaluation of patients referred to the pediatric cardiologist for the evaluation of heart murmur or chest pain. DESIGN: In this prospective study, 106 consecutive outpatients were categorized with no heart disease, possible heart disease, or definite heart disease based on history and physical examination; they then underwent ECG and CXR. Studies were reviewed and the examining cardiologist could change the diagnosis and order an echocardiogram. SETTING: Academic pediatric cardiology practice. RESULTS: In patients thought to have no heart disease, the diagnosis was changed to definite heart disease in four solely on the basis of abnormal CXR or ECG. In 25 patients thought to have possible heart disease, the diagnosis was changed to no heart disease (7) or definite heart disease (5) after review of the CXR and ECG. All 25 patients diagnosed with definite heart disease had this confirmed by abnormal CXR (2), ECG (3), both abnormal CXR and ECG, or echocardiogram (18). CONCLUSIONS: ECG and CXR helped diagnose heart disease in four patients thought to have no heart disease, helped to rule out lesions in seven patients with possible heart disease, helped diagnose heart disease in five patients thought to have possible heart disease, and helped confirm heart disease in nine patients. In these days of cost containment, routine ECG and CXR continue to be valuable tools for the pediatric cardiologist in evaluation of patients with heart murmurs or chest pain.