药学院贫困生状态-特质焦虑问卷调查结果分析

应用状态-特质焦虑量表对药学院131名贫困大学生进行调查评定,结果发现贫困生与一般人群在特质焦虑水平上差异无显著性意义,而在状态焦虑水平出现显著性差异。提出应加强贫困大学生心理健康教育,完善社会资助体系,帮助贫困生摆脱焦虑情绪。     

HPLC法测定紫茉莉根中葫芦巴碱的含量

目的测定紫茉莉根中葫芦巴碱的含量.方法采用 HPLC法,色谱柱:Inertsil NH2Columns(250× 4.6 mm,5 μ m,迪马公司);流动相:乙腈-水(80:20);流速:0.8 mL· min-1;检测波长:265 nm;柱温:30 ℃.结果葫芦巴碱在 0.1872～ 0.9360 μ g之间与峰面积呈良好线性关系,r=0.9996;葫芦巴碱的平均回收率为 99.6 %,RSD=1.76%(n=6).结论该方法分离效果好,简便、准确,可用于紫茉莉根的初步质量控制.     

人血浆中氯氮平及去甲氯氮平的固相萃取HPLC测定

建立了HPLC法测定人血浆中的氯氮平及去甲氯氮平含量.采甩C18柱,流动相为甲醇-水-三乙胺-乙酸(600:400:4:2),检测波长254nm,固相萃取法处理血样,地西泮为内标.氯氮平及去甲氯氮平在50～2000ng/ml范围内线性关系良好.平均方法回收率分别为103.1%和102.1%.     

老龄大鼠膀胱生理特性改变的实验研究

目的:探讨老龄大鼠膀胱生理特性的改变及其可能机制.方法:雌性Wistar大鼠分为年轻成年组(6月龄)和老龄组(24月龄),通过在体充盈期膀胱测压,判定逼尿肌不稳定的发生率,比较两组最大膀胱容量和漏尿点压的差异;在水浴条件下,分别测量两组新鲜逼尿肌条的自发收缩频率、应力作用下的反应张力、对电刺激的反应强度等指标,并对实验结果进行统计学分析.结果:与年轻成年组相比,老龄大鼠表现为更易出现逼尿肌不稳定;麻醉条件下最大膀胱容量显著增加(P＜0.01),漏尿点压无明显差异;自发收缩频率显著增高(P＜0.05).老龄大鼠逼尿肌条在应力增加时,张力上升缓慢;应力降低时,张力下降迅速.应力改变时电刺激诱发收缩表现为与单纯应力改变时的张力改变相似,张力增加值在两组间元差异.结论:老龄膀胱功能的改变与自身逼尿肌兴奋性增加、高顺应性、弹性降低有关,肌源性改变是其主要因素.     

角膜内皮细胞计和超声角膜测厚仪测量角膜中央厚度的比较

目的　比较角膜内皮细胞计和超声角膜测厚仪测量的角膜厚度值之间的差异性。方法　分别应用角膜内皮细胞计和超声角膜测厚仪对 5 3例 (10 6眼 )的中央角膜厚度进行测量 ,对两组测量值进行配对t检验、直线相关分析 ,并对两种仪器三次测量值行组内相关系数分析。结果　应用超声角膜测厚仪检测的角膜厚度均值为 (5 2 1 77± 32 16 ) μm ,角膜内皮细胞计数计检测的均值为 (5 15 4 8± 30 39) μm ,后者显著低于前者 (P<0 0 0 1) ,两者呈显著正相关 (r=0 938,P <0 0 0 1) ,超声法和角膜内皮细胞计法测厚的组内相关系数分别为0 95和 0 93。结论　角膜内皮细胞计测量值低于超声测厚仪测量值 ,但从临床角度来看 ,这种差异并不能否定角膜内皮计用于角膜测厚的可靠性 ,角膜内皮细胞计测量值的一致性甚至超过了超声测厚仪 ,并有非接触性检查的优点。角膜内皮细胞计和超声测厚仪均能为我们提供较为准确的角膜厚度值 ,但临床测量时应当知道两种检查方法之间的差异 ,并认识到各自的优劣 ,才能更好地选择应用不同的设备。对于随访患者应采用同一种仪器来进行检测才具有更好的可比性     

Different classes of antibiotics exhibit disparate negative impacts on the therapeutic efficacy of immune checkpoint inhibitors in advanced non-small cell lung cancer patients

It has been reported that antibiotics (ATBs) have adverse effect on the efficacy of treatment with immune checkpoint inhibitors (ICIs) in cancer patients. Since different classes of ATBs have different antibacterial spectrum, we aimed to study whether all ATBs had similar or different negative effects on the clinical outcomes of ICIs in patients with advanced non-small cell lung cancer (NSCLC). Patients with advanced NSCLC who received ICIs were included in this retrospective study and grouped by the class of ATBs they had used around the ICIs treatment time. The overall survival (OS) and the progression free survival (PFS) of patients among these groups were compared using Kaplan-Meier method and Cox proportional hazards model. A total of 148 eligible patients were enrolled, and 80 patients used ATBs. The results indicated that quinolones had no significant negative consequence on the clinical outcomes, while β-lactams significantly shortened the OS and PFS of patients. Furthermore, patients exposed to the combination of β-lactams and quinolones suffered the worst OS and PFS. Moreover, the subgroup analysis of β-lactams revealed that only penicillins, but not carbapenems and cephalosporins, markedly reduced both OS and PFS. In addition to the class of ATBs used, the time frame of ATBs used also affected the clinical outcomes of ICIs therapy. Patients receiving ATBs within 60 days prior to and 30 days after the initiation of ICI treatment had significantly shorter OS and PFS compared with those who did not use ATBs. This study demonstrated that different classes of ATBs had disparate negative impacts on the clinical outcomes, and the use of β-lactams, especially penicillins, should be avoided in advanced NSCLC patients who are receiving or scheduled to receive ICIs within 60 days.     

破骨细胞钠氢转运蛋白集中于线粒体中表达

目的：把RAW264.7和人外周血CD14+ 诱导成破骨样细胞,检测破骨细胞在成熟过程中钠氢转运蛋白2（Na+-H+ An－tiporter 2,NHA2）的转录表达情况,并定位其表达位置。方法：细胞因子RANKL、M-CSF在体外诱导RAW264.7和人外周血CD14+细胞发育成破骨样细胞;RT-PCR检测NHA2在诱导过程中转录水平上所发生的变化;Western blot检测诱导前后此蛋白表达情况;细胞原位荧光染色对其表达进行细胞精确定位。结果：RAW264.7和人外周血CD14+ 细胞被RANKL、M-CSF诱导成破骨样细胞;RT-PCR结果显示NHA2随着破骨细胞的分化成熟转录水平不断加强;Western blot结果显示此蛋白只表达于成熟的破骨细胞中;细胞原位荧光染色结果显示这个蛋白主要表达于成熟破骨细胞的线粒体中。结论：NHA2作为盐离子载体主要表达于成熟破骨细胞的线粒体中,通过控制线粒体盐离子的代谢平衡来影响破骨细胞的发育分化     

利用微信公众号宣传平台提升门诊服务质量的实践探讨

为深入贯彻落实"互联网+医疗健康"便民惠民活动,不断优化门诊服务流程,提高服务效率与质量,门诊管理者积极探索,创新服务模式,以微信公众号建设为突破口,主动宣传国家医改新政策,落实分级诊疗,提升服务水平。通过线上服务模式,加强与患者的沟通,提供贯穿诊前、诊中、诊后全流程的信息化便民服务,有效缓解了门诊"三长一短"的现象,实现了患者有序就医,患者满意度提高。尤其在疫情防控期间,对于减少人员聚集、保障就医安全等方面起到了积极的作用。     

肥胖相关脂肪性肝病的影响因素

随着饮食结构和生活方式的改变,肥胖症、嗜肝病毒感染和酒精滥用共同成为当代肝病的三大病因,肥胖症及其伴随的胰岛素抵抗直接参与非酒精性脂肪性肝病（NAFLD）及其重要类型脂肪性肝炎（NASH）的发病。全球尤其是亚太地区肥胖流行已成为近十年NAFLD患病率显著增加的主要原因,因此当前在很多国家,NAFLD已成为慢性肝病最常见原因。然而亚洲一些NAFLD患者通常并无肥胖,这可能与各种族间超重和肥胖的定义不同有关。另外,并非所有肥胖患者都并发脂肪肝。提示体内增多的脂肪对肝脏并非都有害,仅某些部位的脂质沉积易引起代谢紊乱和肝脏受损。     

Two important factors affecting the prognosis of patients treated with sorafenib followed by regorafenib for advanced hepatocellular carcinoma

ObjectiveTo investigate the effects of hand–foot syndrome (HFS) and fatigue on disease progression and survival in patients treated with sorafenib followed by regorafenib for advanced hepatocellular carcinoma.MethodsA retrospective analysis of patients with advanced hepatocellular carcinoma treated with sorafenib in our hospital from 1 October 2018 to 31 October 2021 was performed, and clinical and pathological data and follow-up results were obtained. Patients were divided into groups according to the severity of HFS and fatigue. Survival analysis among the groups was performed using the Kaplan–Meier method, continuous variables were analyzed using the t-test, and factors associated with survival were evaluated using multivariate Cox regression analysis.ResultsThe study included 150 men and 23 women with a mean age of 60.77 years (range: 40–85 years). The median overall survival (OS), progression-free survival (PFS), and time to tumor progression (TTP) increased with increasing severity of HFS. Conversely, the median OS, PFS, and TTP decreased with increasing severity of fatigueConclusionHFS and fatigue were independent risk factors affecting TTP, PFS, and OS among patients treated with sorafenib followed by regorafenib for advanced hepatocellular carcinoma.