升压治疗急性脑缺血再灌注损伤保护作用的实验研究

目的:探讨升压治疗局灶性脑缺血的保护作用及治疗的维持时间。方法:采用大鼠局灶性脑缺血再灌注模型,40只大鼠随机分为五组:模型对照组(A)、升压维持15min组(B)、维持45min组(C)、维持90min组(D)和120min组(E),观察各组神经功能评分、脑梗死体积和病理学改变。结果:神经功能缺损评分B、C、D组明显低于A组;B、C、D、E各组脑梗死体积明显低于A组,C、D、E组明显低于B组;各治疗组组织病理学改变比对照组轻,B组最轻。结论:缺血3h再灌注时,升压维持时间在2h以内对脑损伤有保护作用,升压最佳维持时间是15min。     

急性白血病bcl-2表达及与耐药的关系

t(14;18)染色体易位产生融合基因bcl-2/IgH,bcl-2高表达可阻抑细胞凋亡的发生,bax可拮抗bcl-2的抗凋亡效应,起着加速细胞凋亡的作用.bcl-2和bax基因的紊乱是AL(急性白血病)形成与发展的原因之一.这些细胞凋亡调控基因不仅参与了白血病的形成,还与白血病的某些临床特征、疗效及预后因素密切相关.在治疗bcl-2相关肿瘤时,若能联合使用bcl-2反义核酸和其它诱导细胞死亡制剂,可使肿瘤细胞对后者的敏感性增加,从而提高治疗效果.     

2型糖尿病并血管病变患者免疫功能研究

目的 :探讨 2型糖尿病并血管病变患者血液中细胞免疫、体液免疫和C反应蛋白 (CRP)的变化。方法 :采用流式细胞术和免疫比浊法测定 42例糖尿病血管病变患者血液细胞免疫参数CD3、CD4、CD8、CD4/CD8、B细胞和NK细胞、血清免疫球蛋白 (IgG、IgA、IgM)、补体 (C3、C4)等体液免疫参数和作为炎症介质的CRP ,并与5 0例 2型糖尿病无血管病变者及 46例正常对照组比较。结果 :2型糖尿病无论是否并发血管病变 ,与正常对照组比较CD3、CD4、CD4/CD8、B、NK显著性减低 (P <0 .0 1) ,IgA、IgM、C3、C4、CRP显著升高(P <0 .0 1) ;并发血管病变组与不并发血管病变组比较C4、CRP显著增高 (P <0 .0 1)。结论 :2型糖尿病患者无论是否并发血管病变 ,机体细胞免疫和体液免疫都异常 ,且炎症介质CRP升高 ,并发血管病变患者机体的炎症介质进一步升高。     

心肌细胞膜通道电流及其动力学研究

建立了心肌细胞膜离子通道钠电流，钾电流和钙电流的动力数学模型，探讨了离子通道动力学机制，为进一步整合各离子电流数学模型用以研究动作电位全过程和为动物实验以及临床研究提供理论基础。     

慢性心功能不全时经主动脉移植干细胞的可行性研究

心肌梗死或/和慢性心功能不全时,心脏干细胞移植是一项颇有应用前景的新的治疗技术。但是,对于非缺血性心脏病所致的慢性心功能不全,目前所采用的移植路径有一定的局限性。本试验拟研究慢性心功能不全时,封堵主动脉窦上部经主动脉根部移植干细胞是否可行。阿霉素所致的慢性心功能不全兔模型,采用双球囊封堵主动脉窦上部经主动脉根部自体移植骨髓单核细胞,4周后评价心功能。在封堵主动脉窦上部经主动脉根部移植干细胞时,无1例因移植手术死亡。双球囊充盈时造影显示,主动脉窦显影良好,大部分情况下,左、右冠状动脉可显影。骨髓单核细胞移植4周后,左室射血分数改善明显(P=0 .0 34)。所以,慢性心功能不全时,封堵主动脉窦上部经主动脉根部移植干细胞安全可行。     

强迫症疾病行为的精神病理机制研究

目的对研究获得的强迫症疾病行为特征探索其精神病理学机制。方法以符合CCMD-Ⅱ的门诊强迫症患者50例为对象，选取非精神科病人50例组成对照，应用“强迫症疾病行为特征量表（OBPS）”，确定其有无强迫症疾病行为。同时调查研究组与对照组的人格特质、偶发事件、应对策略等方面，研究这些因素在疾病发生、发展中的作用。结果①研究组全部符合李一高量表的疾病行为特征，积分明显高于对照组（t=26．480，P〈0．01）；②强迫症患者人格当中的强迫质偏高，积分明显高于对照组（t=15．93，P〈0．01）；③研究组中绝大多数患者存在偶发事件，而对照组中偶发事件的发生率低于研究组（X^2=21．374，P〈0．01）；④应对策略特征方面研究组不成熟应对方式的应用明显多于对照组。结论强迫神经症形成中人格特质作为基础，偶发事件起到启动对“不完全的恐怖”，错误的应对策略不断强化病感，促进疾病形成。李一高强迫症疾病行为理论的3项内容，特征性的反映了强迫症的疾病行为。     

Radioprotective Effect of Whey Hydrolysate Peptides against γ-Radiation-Induced Oxidative Stress in BALB/c Mice

Radiation therapy is widely used in the treatment of tumor diseases, but it can also cause serious damage to the body, so it is necessary to find effective nutritional supplements. The main purpose of this study is to evaluate the protective effect of whey hydrolysate peptides (WHPs) against ⁶⁰Coγ radiation damage in mice and explore the mechanism. BALB/c mice were given WHPs by oral gavage administration for 14 days. Then, some mice underwent a 30-day survival test after 8 Gy radiation, and other mice received 3.5 Gy radiation to analyze the changes in body weight, hematology and bone marrow DNA after three and 14 days. In addition, through further analysis of the level of oxidative stress and intestinal barrier function, the possible mechanism of the radioprotective effect of WHPs was explored. The study found WHPs can prolong survival time, restore body weight, and increase the number of peripheral blood white blood cells and bone marrow DNA content in irradiated mice. In addition, WHPs can significantly improve the antioxidant capacity, inhibit pro-inflammatory cytokines and protect the intestinal barrier. These results indicate that WHPs have a certain radioprotective effect in mice, and the main mechanism is related to reducing oxidative damage.     

Evaluation of Serum Zinc Status of Pregnant Women in the China Adult Chronic Disease and Nutrition Surveillance (CACDNS) 2015

Objective: The purpose of this study was to evaluate serum zinc status of pregnant women in the China Adult Chronic Disease and Nutrition Surveillance (CACDNS) in 2015–2016. Methods: A total of 7147 apparently healthy pregnant women were randomly selected in 302 national monitoring sites. Information on age, race, residence region, education, pregnancy, and family income per annum was collected, and the concentration of serum zinc was determined. The evaluation of serum zinc status was further performed according to the recommendations by the International Zinc Nutrition Consultative Group (IZiNCG). Results: The median concentration of serum zinc was 858.9 μg/L with an interquartile range (IQR) of 712.9 μg/L and 1048.9 μg/L, while the overall prevalence of zinc deficiency was 3.5% with a 95% confidence interval (CI) of 3.0% and 3.9%. Serum zinc status of pregnant women changed greatly in the different categories, particular in pregnancy and family income per annum (p < 0.05), but no significant difference was observed in the prevalence of zinc deficiency (p > 0.05). Conclusions: The lower prevalence of zinc deficiency generally indicated a better zinc status for pregnant women in the CACDNS in 2015–2016. However, a well-designed evaluation system of zinc status for pregnant women should be continually optimized and improved by inducing more parameters such as biochemical, dietary, or functional indicators.