Vulnerable Sites and Changes in Mucin in the Rat Small Intestine After Non-steroidal Anti-inflammatory Drugs Administration

Background and Aims  

The location of mucosal damage and changes in mucin content in the rat small intestine following administration of non-steroidal anti-inflammatory drugs (NSAIDs) have not been well elucidated.     

Evaluation of Enhanced Peritoneum Permeability in Methylglyoxal-treated Rats as a Diagnostic Method for Peritoneal Damage

Purpose As peritoneal damage in long-term peritoneal dialysis therapy is a major problem correlated to patient prognosis, diagnosis of peritoneal damage is important. To develop a diagnostic method for peritoneal damage, we focused on hyperpermeability across the peritoneum in a pathogenic peritoneal damage condition. In this study, disposition characteristics of an intraperitoneally injected marker substance in peritoneal damaged rats were analyzed. Materials and Methods Peritoneal damaged rats were prepared by intraperitoneal injection of a glucose degradation product, methylglyoxal (MGO), for five or ten consecutive days. Phenolsulfonphthalein (PSP), as a marker substance, was intraperitoneally or intravenously injected into MGO-treated rats. Subsequently, the PSP disposition characteristics were pharmacokinetically analyzed. Results In both cases of 5 and 10 days treatment of MGO, absorption of PSP after intraperitoneal injection was significantly enhanced. Plasma concentration and urinary excretion of PSP in MGO-treated rats were also higher than those in saline-treated rats in the early phase. On the contrary, there was no significant difference in terms of the pharmacokinetic parameters of intravenously injected PSP in saline- or MGO-treated rats. These results indicated that intraperitoneally injected MGO primarily acts on the peritoneal membrane; therefore, the peritoneal permeability of the marker substance was enhanced. Conclusion We demonstrated that pharmacokinetic analysis of peritoneum permeability might be a potent diagnostic method for peritoneal damage in experimental animals and patients receiving peritoneal dialysis.     

Cerebral protective and cognition-improving effects of sinapic acid in rodents

We previously demonstrated that tenuifoliside B and 3,6'-disinapoylsucrose in Polygalae Radix, the root of Polygala tenuifolia WILLDENOW, inhibited potassium cyanide (KCN)-induced hypoxia and scopolamine-induced memory impairment in mice. Because both ingredients have a common sinapoyl moiety in their structure, we inferred that the sinapoyl moiety could inhibit hypoxia and memory impairment. In the present study to clarify the hypothesis, sinapic acid inhibited KCN-induced hypoxia and scopolamine-induced memory impairment as well as tenuifoliside B and 3,6'-disinapoylsucrose did. In addition, sinapic acid inhibited decompression- or bilateral carotid artery ligation-induced hypoxia (or mortality) and CO2-induced impairment in mice, and basal forebrain lesion-induced cerebral cholinergic dysfunction (decreases in acetylcholine concentration and choline acetyltransferase activity) in rats. These results, taken together, suggest the possibilities that sinapic acid is not only a very important moiety in the pharmacological activities of tenuifoliside B and 3,6'-disinapoylsucrose but also a candidate for a cerebral protective and cognition-improving medicine.     

The role of objective plane angulation on the mandibular image using cross-sectional tomography

Cross-sectional jaw images in the buccolingual direction obtained by conventional or computerized tomography are used in the image diagnosis of dental implant treatment. This study was performed to clarify the subjective image quality of the mandibular depiction by shifting the angles of the tomographic objective plane. A panoramic machine with a linear tomographic function was used to obtain cross-sectional tomographic images on bilateral first molar regions of 10 dried human mandibles. The angles of tomographic objective planes were shifted horizontally within a range of +/- 20 degrees at intervals of 5 degrees from the tomographic objective plane, which was automatically determined. The image qualities of 4 anatomical structures-alveolar crest, buccal and lingual cortical bone, and mandibular canal-were subjectively scored on a 5-point scale method. As a result, the permitted tomographic objective angles were from -1.7 degrees to 2.5 degrees, a range of 4.2 degrees for all 4 anatomical structures. When this result was compared with a previous geometric result, the permitted range of the angles was quite narrow. The tomographic objective angles should be manually set in accordance with an optimal tomographic plane for individual patients by using the positioning technique in linear tomography.     

Scanning electron microscope of for endophthalmitis case

PURPOSE: The purpose of this study was to demonstrate the usefulness of the scanning electron microscope in examining the posterior capsule and an intraocular lens (IOL) in an endophthalmitis patient. METHODS: The patient was a 77-year-old man with diabetes mellitus undergoing radiotherapy for prostatic carcinoma, and having a history of esophageal cancer. Phacoemulsification, aspiration and IOL insertion by keratotomy were carried out. The operation was performed without complications. The IOL was a one-piece acrylic type. However, after 24 hours, we diagnosed acute bacterial endophthalmitis, because the patient showed visual loss, high intraocular pressure, fibrin formation, and an increase of cells in the anterior chamber. Vitrectomy to remove the IOL and posterior capsule was carried out on the same day. The removed samples were observed by scanning electron microscope. RESULTS: Enterococcus faecalis was detected in the vitreous and aqueous humor. The bacteria adhered to the optic and haptic surfaces and showed biofilm formation. The traces of the end mill cutting line were recognized beside the haptics. However, the posterior capsule showed no bacterial adhesion but had an unusual shape. CONCLUSIONS: We observed the biofilm formation in an IOL removed from an acute bacterial endophthalmitis case. An IOL may be an important canse of bacterial proliferation and adhesion.     

Biofilm formation by Staphylococcus epidermidis on intraocular lens material

PURPOSE: To compare biofilm formation by Staphylococcus epidermidis on different intraocular lens (IOL) materials. METHODS: The S. epidermidis strains, ATCC 12228 (American Type Culture Collection) and ATCC 35984 (biofilm-producer) were used. Biofilms were cultivated on disks of different IOL materials: silicone, PMMA (polymethylmethacrylate), acrylic, or MPC (2-methacryloyloxyethyl phosphorylcholine) surface-modified acrylic. Biofilms were stained with crystal violet (CV) which served as an index of biofilm formation. The bacterial population was enumerated after biofilm homogenization. Biofilms were also examined by scanning electron microscopy (SEM). RESULTS: Among the four materials tested, the least amount of biofilm formed on silicone. Biofilm production was significantly different between acrylic and MPC surface-modified acrylic lenses at 48 hours (P<0.05-0.01). The bacterial populations were significantly different between acrylic and silicone over 72 hours (P<0.05-0.01). The population on acrylic and MPC surface-modified acrylic continued to increase over 72 hours. The biofilm was recognized after a 24-hour incubation. Rates of biofilm-positive SEM fields, which were defined as being occupied by biofilm over at least half of the area, were significantly different between acrylic and silicone at 72 hours (P<0.05-0.01). CONCLUSIONS: S. epidermidis formed biofilm most intensely on acrylic among the four IOL materials tested. MPC surface-modified acrylic has a preventive effect on biofilm formation.     

Cognitive improving and cerebral protective effects of acylated oligosaccharides in Polygala tenuifolia

We studied the cognitive improving and cerebral protective constituents in the roots of Polygala tenuifolia Willdenow, a well-known Chinese traditional medicine prescribed for amnesia, neurasthenia, palpitation, noctural emission and insomnia. Tenuifoliside B (1), which is one of the acylated oligosaccharides in the roots of P. tenuifolia, showed the cerebral protective effect on potassium cyanide (KCN)-induced anoxia in mice, widely used as an animal model for cerebrovascular disease, and also had an ameliorative effect on the scopolamine-induced impairment of performance in passive avoidance task in rats. Compound 1 significantly enhanced oxotremorine-induced tremors in mice, suggesting that it ameliorated the scopolamine-induced impairment of passive avoidance response by enhancing the cholinergic system. These findings show that compound 1 has cognitive improving and cerebral protective effects.     

Voluntary Control of Electrodermal Activity in Relation to Imagery and Internal Perception Scores

feedback of spontaneous Electro dermal fluctuation in human subjects increased their in frequency of occurrence relative to a non-contingent feedback control group. The effect was facilitated for subject having high imagery und/or high internal perception as measured by the Suphian Scale of Imagery (SSI) and Sophian Scale of Internal Perception (SSP) respectively. These data suggest the existence of individual differences in self-control of autonomic responses.     

Monoclonal antibodies that distinguish antigenic variants of canine parvovirus

Canine parvovirus (CPV) is classified as a member of the feline parvovirus (FPV) subgroup. CPV isolates are divided into three antigenic types: CPV type 2 (CPV-2), CPV-2a, and CPV-2b. Recently, new antigenic types of CPV were isolated from Vietnamese leopard cats and designated CPV-2c(a) or CPV-2c(b). CPV-2c viruses were distinguished from the other antigenic types of the FPV subgroup by the absence of reactivity with several monoclonal antibodies (MAbs). To characterize the antigenicity of CPV-2c, a panel of MAbs against CPV-2c was generated and epitopes recognized by these MAbs were examined by selection of escape mutants. Four MAbs were established and classified into three groups on the basis of their reactivities: MAbs which recognize CPV-2a, CPV-2b, and CPV-2c (MAbs 2G5 and 20G4); an MAb which reacts with only CPV-2b and CPV-2c(b) (MAb 21C3); and an MAb which recognizes all types of the FPV subgroup viruses (MAb 19D7). The reactivity of MAb 20G4 with CPV-2c was higher than its reactivities with CPV-2a and CPV-2b. These types of specificities of MAbs have not been reported previously. A mapping study by analysis of neutralization-resistant mutants showed that epitopes recognized by MAbs 21C3 and 19D7 belonged to antigenic site A. Substitution of the residues in site B and the other antigenic site influenced the epitope recognized by MAb 2G5. It was suggested that the epitope recognized by MAb 20G4 was related to antigenic site B. These MAbs are expected to be useful for the detection and classification of FPV subgroup isolates.