一种“新”的不稳定性胸腰椎压缩劈裂骨折

本文报道9例在CT 和X 线平片上都有同一类型病理变化、发病机理和临床过程的胸腰椎压缩劈裂骨折.CT 上的共同病理变化为:(1)骨折椎的上椎间盘损伤;(2)椎体上1/2的压缩骨折:(3)椎体下1/2的矢状劈裂骨折:(4)椎管前部出现椎体中柱骨折块:(5)椎板骨折.通过对本类骨折的力学分析,作者认为这是—种独立的不稳定性爆裂骨折,且有神经性不稳定的特征.临床治疗应切开复位,并使用有撑开效应的固定器.     

非胰岛细胞瘤性低血糖的研究进展

非胰岛细胞瘤性低血糖(NICTH)是一种胰岛细胞瘤以外的其他肿瘤导致的低血糖症。肿瘤组织分泌过多的胰岛素样生长因子Ⅱ(IGF-Ⅱ)的一种前体形式物质,因其相对分子质量较大,大多数文献称之为big-IGF-Ⅱ,它能产生类胰岛素样活性,故目前认为是导致患者反复出现低血糖的病因。诊断上主要依靠高水平的big-IGF-Ⅱ测定。NICTH的治疗方式较多,但治愈手段主要为手术切除肿瘤组织。现就NICTH的发病机制、临床表现、诊断及治疗等方面的研究进展予以综述。     

Novel Amdoparvovirus Infecting Farmed Raccoon Dogs and Arctic Foxes

A new amdoparvovirus, named raccoon dog and fox amdoparvovirus (RFAV), was identified in farmed sick raccoon dogs and arctic foxes. Phylogenetic analyses showed that RFAV belongs to a new species within the genus Amdoparvovirus of the family Parvoviridae. An RFAV strain was isolated in Crandell feline kidney cell culture.     

医用电子仪器专业存在问题与解决方案的探索

医用电子仪器专业是一门新兴的交叉边沿性学科，分析近年来医电专业开设过程中所存在的问题．并对解决方案进行了探讨。     

circ_0006089 promotes gastric cancer growth,metastasis, glycolysis,and angiogenesis by regulating miR‐361‐3p/TGFB1

Circular RNA (circRNA) participates in a variety of pathophysiological processes, including the development of gastric cancer (GC). However, the role of circ_0006089 in GC progression and its underlying molecular mechanism need to be further revealed. Quantitative real‐time PCR was utilized for detecting circ_0006089, microRNA (miR)‐361‐3p and transforming growth factor‐β1 (TGFB1) expression. The interaction between miR‐361‐3p and circ_0006089 or TGFB1 was confirmed using a dual‐luciferase reporter assay and an RNA immunoprecipitation (RIP) assay. Cell proliferation, metastasis, apoptosis, and angiogenesis were determined using colony formation assay, EdU assay, transwell assay, flow cytometry, and tube formation assay. Cell glycolysis was evaluated by detecting glucose consumption, lactate production, and ATP levels. In addition, western blot (WB) analysis was used to measure protein expression. Xenograft tumor models were used to assess the effect of circ_0006089 knockdown on GC tumorigenesis. circ_0006089 had been found to be upregulated in GC tissues and cells, and it could act as an miR‐361‐3p sponge. circ_0006089 knockdown suppressed GC proliferation, metastasis, glycolysis, angiogenesis, and increased apoptosis, while this effect could be revoked by miR‐361‐3p inhibitor. TGFB1 was targeted by miR‐361‐3p, and its overexpression reversed the effects of miR‐361‐3p on GC cell function. Also, circ_0006089 promoted TGFB1 expression via sponging miR‐361‐3p. Animal experiments showed that silenced circ_0006089 inhibited GC tumorigenesis through the miR‐361‐3p/TGFB1 pathway. Our results revealed that the circ_0006089/miR‐361‐3p/TGFB1 axis contributed to GC progression, confirming that circ_0006089 might be a potential therapeutic target for GC.     

原发性肝癌Twist基因表达的研究

目的：研究Twist基因在人原发性肝癌（primary liver cancer,PLC）组织中的表达及其临床意义。方法：应用免疫组织化学SABC染色方法检测45例PLC组织及癌旁组织中Twist蛋白并采用RT-PCR对TwistmR-NA的表达进行分析研究。结果：PLC组织、癌旁组织及正常肝组织中Twist的阳性表达率分别为80.0%、57.8%和22.2%。PLC和癌旁组织与正常对照肝组织中Twist的表达相比较差异均有统计学意义,P〈0.05。肿瘤包膜也可见Twist表达。RT-PCR检测Twist结果显示,PLC组织中Twist mRNA表达显著上调。结论：PLC组织中高表达转录因子Twist对肿瘤的发生、发展起着重要作用。     

Pan‐cancer analysis reveals sex‐specific signatures in the tumor microenvironment

The processes of cancer initiation, progression, and response to therapy are affected by the sex of cancer patients. Immunotherapy responses largely depend on the tumor microenvironment (TME), but how sex may shape some TME features, remains unknown. Here, we analyzed immune infiltration signatures across 19 cancer types from 1771 male and 1137 female patients in The Cancer Genome Atlas to evaluate how sex may affect the tumor mutational burden (TMB), immune scores, stromal scores, tumor purity, immune cells, immune checkpoint genes, and functional pathways in the TME. Pan‐cancer analyses showed higher TMB and tumor purity scores, as well as lower immune and stromal scores in male patients as compared to female patients. Lung adenocarcinoma, lung squamous carcinoma, kidney papillary carcinoma, and head and neck squamous carcinoma showed the most significant sex biases in terms of infiltrating immune cells, immune checkpoint gene expression, and functional pathways. We further focused on lung adenocarcinoma samples in order to identify and validate sex‐specific immune cell biomarkers with prognostic potential. Overall, sex may affect the tumor microenvironment, and sex‐specific TME biomarkers may help tailor cancer immunotherapy in certain cancer types.     

脊柱外科手术患者临床数据库系统应用体会

南京鼓楼医院脊柱外科于2002年建立并开始应用脊柱外科手术患者临床数据库系统.文章回顾总结脊柱外科手术患者临床数据库系统的应用体会,供参考.