VEGF—C mRNA在口腔鳞癌中的表达

目的：探讨血管内生长因子－Ｃ（ＶＥＧＦ－Ｃ）ｍＲＮＡ在正常口腔粘膜、白斑及鳞癌等组织中的表达。方法：ＴＲ－ＰＣＲ法检测ＶＥＧＦ－Ｃ ｍＲＮＡ的表达。结果：鳞癌组织ＶＥＧＦ－Ｃ ｍＲＮＡ表达明显高于正常口腔粘膜及白斑（Ｐ〈０．０１）;鳞癌表达与病理分级（Ｐ〈０．０１）、淋巴结转移（Ｐ〈０．０１）密切相关,与临床分期无关（Ｐ〉０．０５）。结论：ＶＥＧＦ－Ｃ对鳞癌生长及转移有促进作用,有望作为检测淋巴管     

青光眼滤过术后透明角膜切口白内障超声乳化摘除人工晶体植入术

介绍经透明角膜切口的青光眼滤过术后白内障超声乳化摘除人工晶体植入的方法及疗效。方法：对１８例１８眼施行手术,上方透明角膜内１ｍｍ处做３．２ｍｍ切口,。超声乳化白内障摘除后扩大切口中至５．５－６ｍｍ,植入人工晶体。结果１８眼术后视力全部提高,术中术后无严重并发症。     

止消通脉宁对糖尿病大鼠肾脏胶原非酶糖化的影响

采用STZ糖尿病大鼠模型 ,在 6 ,12周分别观察肾脏组织发生非酶糖基化的情况 ,并观察止消通脉宁及氨基胍的作用。结果发现不同病程STZ糖尿病大鼠肾脏组织的胶原含量和非酶糖基化早期产物 (5 HMF)及胶原相联荧光值均明显高于正常对照组 ,并随病程的延长而增高。止消通脉宁有阻断肾脏组织非酶糖基化的作用 ,而对 5 HMF的作用优于氨基胍。说明高糖环境下肾脏组织可发生非酶糖基化 ,止消通脉宁有抑制肾脏组织非酶糖基化的作用 ,是其防治糖尿病肾病的作用机理之一     

三氧化二砷对肝癌细胞系HepG2生长及核基质蛋白的影响

目的 探讨三氧化二砷对人肝癌细胞系ＨｅｐＧ２的生长及核基质蛋白的影响。方法 应用生长指数分析和高分辨ＳＤＳ－聚丙烯酰胺交双向电泳比较用Ａｓ２Ｏ３处理后的ＨｅｐＧ２细胞主核基质蛋白的改变。结果 Ａｓ２Ｏ３抑制ＨｅｐＧ２的生长,双向电泳显示经Ａｓ２Ｏ３处理后ＨｅｐＧ２细胞内出现新的核基质蛋白,并且该蛋白是细胞特异性的。结论 核基质蛋白是某些抗癌药物的作用靶子,Ａｓ２Ｏ３抑帛 癌细胞的生长可望有用于临床     

High Expression of Insulin-like Growth Factor Ⅱ (IGF-Ⅱ) Using Bac-to-Bac Expression System

Ｈｕｍａｎｉｎｓｕｌｉｎ ｌｉｋｅｇｒｏｗｔｈｆａｃｔｏｒ Ⅱ (ＩＧＦ Ⅱ )ｉｓａ 6 7ａｍｉｎｏａｃｉｄｓｉｎｇｌｅ ｃｈａｉｎｐｅｐｔｉｄｅ ,ｗｈｉｃｈｉｓｉｎｖｏｌｖｅｄｉｎｆｅｔａｌｇｒｏｗｔｈａｎｄｄｅｖｅｌｏｐ ｍｅｎｔ[1] .Ｔｈｅｇｒｏｗｔｈ ｐｒｏｍｏｔｉｎｇｐｒｏｐｅｒｔｉｅｓｏｆＩＧＦ Ⅱ ,ａｓｗｅｌｌａｓｔｈｅｉｒｓｔｒｕｃｔｕｒａｌｈｏｍｏｌｏｇｙｔｏｐｒｏｉｎｓｕｌｉｎａｎｄＩＧＦ Ⅰａｒｅｒｅｆｌｅｃｔｅｄｉｎｔｈｅｎａｍｅｏｆｔｈｅｐｅｐｔｉｄｅｓ .Ｉｔｉｓａｌｓｏｈｅｌｐｆ…     

改良的Black法功能性修复双侧唇裂18例分析

推广双侧唇裂的功能性修复。方法：应用改良的Ｂｌａｃｋ法。结果：１８例双侧唇裂患儿经６－１８个月的术后随访,均后获得满意的动态及静态效果。结论无论完全性或不完全性双侧唇裂,只有功能性修复,才可获得良好的前唇部肌肉及功能。     

急性坏死型胰腺炎的B超与CT诊断比较(附38例分析)

目的 评价超声及ＣＴ诊断急性坏死型胰腺炎（ＡＮＰ）的价值。方法 将３８例急性胰腺炎患者手术前的超声及ＣＴ诊断资料与手术病理诊断进行对照,分析超声及ＣＴ诊断ＡＮＰ的敏感性、特异性、预测精确性。结果 ３８例患者,按手术病理诊断,３０例为ＡＮＰ,７例为急性水肿型胰腺炎,１例化脓性胆囊炎胰腺未见异常。ＣＴ诊断ＡＮＰ的敏感性９５．８％,特异性８０．０％,阳性预测值９５．８％,阴笥预测值８０．０％,预测精确性     

GNAS gene mutations affecting XLαs and bone health: A long neglected relationship

The GNAS locus is an imprinted site. The α-subunit of the stimulatory G protein (Gsα) and extralarge variant (XLαs) are the two important products of the GNAS locus. The abnormal expression of Gsα is associated with pseudohypoparathyroidism (PHP) and related disorders, including Albright hereditary osteodystrophy (AHO), pseudopseudohypoparathyroidism (PPHP), and progressive osseous heteroplasia (POH). XLαs protein can mimic the catalytic intracellular synthesis of cyclic adenosine monophosphate (cAMP) by Gsα in response to parathyroid hormone (PTH) stimulation, which may be involved in the pathogenesis of PPHP and POH in patients with paternal GNAS defects. A paternally inherited nonsense variant in the first exon of XLαs in an adult patient may be associated with fractures and osteopetrosis. The relationship between the XLαs product of the GNAS locus and bone remodeling may have been overlooked. Here, we summarize the phenotypes of genetic mouse models and clinical cases of XLαs variations and suggest that the abnormal paternal expression of XLαs may be associated with the development of POH and affect osteoblast and osteoclast differentiation.     

Decreased frequency of a novel T-lymphocyte subset,CD3+CD4−CD7+CD57− T cells,in hepatitis B virus-related end-stage liver disease might contribute to disease progression

CD7 and CD57 are related to the differentiation and functional stages of CD8⁺ T cells. However, the role of their combined presence in CD8⁺ T cells in patients with chronic hepatitis B virus (HBV) infection, especially those with end-stage liver disease, remains unclear. Blood samples from healthy volunteers and patients with chronic hepatitis B were analyzed via Luminex assay and ELISA to measure plasma cytokine levels. Further, recombinant IL-22 was used to stimulate peripheral blood mononuclear cells from healthy volunteers, and the frequency of CD3⁺CD4⁻CD7⁺CD57⁻ T cells and apoptosis rates were investigated via flow cytometry. Patients with end-stage liver disease, particularly those with acute to chronic liver failure, showed decreased CD3⁺CD4⁻CD7⁺CD57⁻ T cell frequency. Furthermore, the prevalence of CD3⁺CD4⁻CD7⁺CD57⁻ T cells was negatively correlated with disease severity, prognosis, and complications (ascites). We also observed that IL-22 promoted apoptosis and brought about a decrease in the number of CD3⁺CD4⁻CD7⁺CD57⁻ T cells in a dose-dependent manner. CD3⁺CD4⁻CD7⁺CD57⁻ T cells displayed a B and T lymphocyte attenuator (BTLA)^highCD25^highCD127^high immunosuppressive phenotype and showed low interferon-γ, tumor necrosis factor-α, granzyme A, and perforin expression levels. The present findings will elucidate the pathogenesis of HBV-related end-stage liver disease and aid the identification of novel drug targets.     

贲门部浸润性癌预后的探讨

探讨贲门部浸润性癌的预后;与幽门部浸润性癌进行对比分析。方法贲门部癌５０例,对照组幽门部部５０例,采用临床病理学资料和免疫组织化学方法进行对比分析。结果①临床病理学,Ｃ．Ｃａｓ和Ｐ．Ｃａｓ之间除淋巴结转移（Ｐ〈０．０５）有显著差异外,其它无显著性差异。②生存曲线分析,Ｃ．Ｃａｓ和Ｐ．Ｃａｓ之间,在淋巴结转移（Ｐ〈０．０１）和淋巴管侵入（Ｐ〈０．０５）方面有显著差异;在１００例进行性胃癌当中癌的浸润