全文获取类型
收费全文 | 1753篇 |
免费 | 143篇 |
国内免费 | 121篇 |
专业分类
耳鼻咽喉 | 11篇 |
儿科学 | 20篇 |
妇产科学 | 11篇 |
基础医学 | 189篇 |
口腔科学 | 30篇 |
临床医学 | 205篇 |
内科学 | 264篇 |
皮肤病学 | 11篇 |
神经病学 | 87篇 |
特种医学 | 102篇 |
外科学 | 166篇 |
综合类 | 345篇 |
预防医学 | 145篇 |
眼科学 | 28篇 |
药学 | 192篇 |
1篇 | |
中国医学 | 86篇 |
肿瘤学 | 124篇 |
出版年
2024年 | 8篇 |
2023年 | 20篇 |
2022年 | 36篇 |
2021年 | 75篇 |
2020年 | 49篇 |
2019年 | 46篇 |
2018年 | 55篇 |
2017年 | 51篇 |
2016年 | 44篇 |
2015年 | 75篇 |
2014年 | 87篇 |
2013年 | 69篇 |
2012年 | 100篇 |
2011年 | 135篇 |
2010年 | 72篇 |
2009年 | 42篇 |
2008年 | 62篇 |
2007年 | 74篇 |
2006年 | 101篇 |
2005年 | 69篇 |
2004年 | 90篇 |
2003年 | 128篇 |
2002年 | 128篇 |
2001年 | 100篇 |
2000年 | 71篇 |
1999年 | 52篇 |
1998年 | 17篇 |
1997年 | 35篇 |
1996年 | 11篇 |
1995年 | 16篇 |
1994年 | 18篇 |
1993年 | 4篇 |
1992年 | 13篇 |
1991年 | 11篇 |
1990年 | 5篇 |
1989年 | 9篇 |
1988年 | 7篇 |
1987年 | 6篇 |
1986年 | 5篇 |
1985年 | 4篇 |
1984年 | 2篇 |
1979年 | 2篇 |
1977年 | 2篇 |
1974年 | 2篇 |
1973年 | 1篇 |
1970年 | 1篇 |
1969年 | 1篇 |
1967年 | 2篇 |
1965年 | 1篇 |
1926年 | 1篇 |
排序方式: 共有2017条查询结果,搜索用时 15 毫秒
11.
Guangrui Fan Kun Li Yangyang Pang Youli Zhao Yan Tao Huimin Gui Hanzhang Wang Robert Svatek Ronald Rodriguez Zhiping Wang 《Translational andrology and urology》2021,10(2):692
BackgroundTo investigate the association between single nucleotide polymorphisms (rs10078761, rs12696304, rs2853669, rs16847897, rs2736100, rs10069690) of telomerase gene (TERT) and the risk clinical benign prostatic hyperplasia (BPH) in a Chinese Han population of the Northwest region.MethodsA total of 150 BPH patients and 150 healthy older males from the northwest Chinese Han population were included in this study. The sample size for this unmatched case-control study was estimated by the look-up table method. Meanwhile, the general information and disease data of patients were collected. Age was only collected in healthy control subjects for statistical correction. Genotypes were detected using a multiplex PCR + ligase detection reaction (LDR). Typing results and clinical data were statistically analyzed using multiple linear regression and logistic regression. Pearson correlation was used for Hardy-Weinberg equilibrium.ResultsThe included population is in Hardy-Weinberg equilibrium. There was no significant association between SNP and the risk of BPH by correlation analysis. However, 4 haplotypes (TCTGGT, TCTGTC, TGCCTC, and TGTGTC) were identified as risk factors of BPH by haplotype analysis. The SNP rs2853669 is an independent risk factor for smooth muscle type of hyperplasia. Besides, rs2736100, rs10078761, and rs10069690 which are in linkage disequilibrium are associated with the severity of BPH.ConclusionsPolymorphism of the TERT gene determines the different disease development and pathological manifestations of BPH in the Chinese Han population the Northwest region. 相似文献
12.
13.
目的:在酶标板上进行DNA探针杂交,评价其在检测临床标本中结核杆菌的应用价值。方法:收集71份结核性标本和32份非结核性标本,进行DNA扩增,把扩增产物分别进行电泳和DNA探针杂交,比较其结果。结果:71份结核性标本电泳法阳性率为451%,DNA探针法阳性率为690%;32份非结核性标本二者均为阴性。结论:DNA探针杂交可提高临床标本中结核杆菌的阳性检出率,是结核病早期诊断的一种新方法。 相似文献
14.
目的:探讨使用大剂量干扰素(IFNα)治疗慢性丙型肝炎(CAH)的效果。方法:使用大剂量IFNα,每天600万U,连续投药3周,以后每周3次间断投药21周,共计24周,治疗CAH50例。观察治疗前后血清HCVRNA与ALT的变化,以此作为IFN疗效的判定指标。结果:IFN治疗CAH有效率为68%,停药6个月后有34%复发。结论:IFN的疗效与CAH的基因亚型有关(Ⅲ型疗效显著,Ⅱ型较差),与IFN的剂量无明显关系。 相似文献
15.
Increased reactive oxygen species in familial amyotrophic lateral sclerosis with mutations in SOD1 总被引:1,自引:0,他引:1
Said Ahmed M Hung WY Zu JS Hockberger P Siddique T 《Journal of the neurological sciences》2000,176(2):88-94
Amyotrophic lateral sclerosis (ALS) is a paralytic disorder characterized by degeneration of large motor neurons of the brain and spinal cord. A subset of ALS is inherited (familial ALS, FALS) and is associated with more than 70 different mutations in the SOD1 gene. Here we report that lymphoblast cell lines derived from FALS patients with 16 different mutations in SOD1 gene exhibit significant increase of intracellular reactive oxygen species (ROS) compared with sporadic ALS (SALS) and normal controls (spouses of ALS patients). The ROS generation did not correlate with SOD1 activity. Further, cells incubated with vitamin C, catalase or the flavinoid quercetin significantly reduced ROS in all groups. The catalase inhibitor 3-amino-1,2,4-triazole resulted in a ten-fold increase of ROS in all groups. Neither L-nitroarginine, a nitric oxide synthase inhibitor or vitamin E altered the ROS levels. Thus, these studies suggest that hydrogen peroxide (H(2)O(2)) is a major ROS elevated in FALS lymphoblasts and it may contribute to the degeneration of susceptible cells. Further, we postulate a mechanism by which increased H(2)O(2) could be generated by mutant SOD1. 相似文献
16.
17.
Campos-Góngora E Viader-Salvadó JM Martínez-Rodríguez HG Zuñiga-Charles MA Galindo JM Said-Fernández S 《Archives of medical research》2000,31(2):162-168
BACKGROUND: Entamoeba histolytica forms cyst-like structures (CLS) in PEHPS but not in TYS-33 medium. Sodium dodecyl sulfate [(SDS (0.1%)] dissolves most of them in 10 min, but not natural cysts. Chitin is responsible mainly for cyst wall resistance. Its synthesis depends on Mg(2)+, Mn(2)+, or Co(2)+, whose action is interactive. With the aid of the Simplex method, we analyzed the effect of 20 blends of these cations to find the one that, when added to PEHPS, produced the highest proportion of CLS resistant to 1% SDS (RCLS). METHODS: The concentration of Mg(2)+, Mn(2)+, and Co(2)+ was determined in PEHPS and TYI-S-33 with a flame atomic absorption spectrometer. The proportion of RCLS produced in PEHPS with each ion blend was tested. The CLS and RCLS affinity to fluorescein wheat germ agglutinin (WGA/FITC), which binds chitin, was determined. RESULTS: PEHPS contained a similar concentration of Co(2)+ (0.52 microM) and 3.4 and 1.6 times more Mg(2)+ (798 microM) and Mn(2)+ (3.15 microM) than TYI-S-33, respectively. The proportion of RCLS increased gradually in PEHPS until reaching 3.6 +/- 1.43% with MgCl(2) 1.22 mM, MnCl(2) 14.44 mM, and CoCl(2) 19.44 mM (ion blend No. 20). Both CLS and RCLS bound WGA/FITC. The RCLS formed in the presence of ion blend No. 20 appeared wrinkled. CONCLUSIONS: Mg(2)+, Mn(2)+, and Co(2)+ enhanced the ability of PEHPS to form RCLS, possibly because these ions stimulated their chitin synthesis. Although ion blend No. 20 produced the highest proportion of RCLS, this high ion concentration may be toxic for encysting amebas. 相似文献
18.
The cell killing effects and bystander effects of double suicide gene on pulmonary carcinoma cells were explored. Lung adenocarcinoma cells (A549) were transfected with different liters of adenovirus vector and followed with different concentrations of 5-FC after a recombinant adenovirus vector carrying CD/UPRT gene (Ad-CD/UPRT) was constructed. The cell viability was measured by MTT assay 4 days later. The cell viability was dropped to 30.57 %-8.62 % after 10 MOI of Ad-CD/UPRT transfected and 5-FC (10-1000μg/mL) administration. Furthermore, Ad-CD/UPRT-infected A549 cells showed a profound neighbor cell killing effect in the same methods. These results suggested that Ad-CD/UPRT/5-FC system can effectively suppress growth of lung adenocarcinoma cells, which may provide a novel and powerful candidate for lung cancer gene therapy strategies. 相似文献
19.
目的探讨尿毒症患者甲状旁腺素与营养状况的关系,为延缓慢性肾功能不全进展而改善患者营养状况提供临床依据。方法测定患者血清甲状旁腺素(PTH)、ALB、Hb;使用改良定量主观评估表 MQSGA 评估患者营养状况。结果 PTH 与 MQSGA、ALB,存在相关关系(P<0.05),与 Hb 存在显著相关关系( P<0.001);PTH 与 MQSGA、Hb 之间 Logistic 回归分析(P<0.05)。结论 PTH 是影响尿毒症患者营养状况的重要尿毒素。清除血清 PTH 能改善患者营养状况。 相似文献
20.
现代教育技术在临床课堂教学应用的新模式 总被引:3,自引:1,他引:3
介绍了现代教育技术在临床课堂教学应用的三种模式,旨在加强医学生临床学习能力、临床思维能力和创新素质的培养,进而推动临床教学改革。 相似文献