The experience of disease in cystic fibrosis: the paths to comprehensive care

Cystic Fibrosis is a chronic disease with an extensive impact on family life. The experience of disease is the manner how individuals respond to the disease, assigning meaning meanings and searching for ways to deal with it in their daily lives. The objective of this study was to understand the experience of Cystic Fibrosis in the family context. This is an ethnographic study, performed with families of children assisted at a teaching hospital located in the state of S?o Paulo. Results were divided into the themes past, present and future, and all phases were permeated with the search for the meaning of the disease and social support, the importance of religion and spirituality, and child's socialization. Knowing the experience in the disease and the social network is indispensible when planning comprehensive care. This is an innovative approach in health care for chronic diseases.     

急性暴发型溃疡性结肠炎合并特发性血小板减少性紫癜1例

患者, 男, 19岁, 因发热15 d, 便血8 d入院. 典型的临床表现结合实验室检查、腹部平片和结肠镜下所见诊断为急性暴发型溃疡性结肠炎(UC). 入院后患者血小板进行性下降, 排除DIC等引起, 结合骨髓穿刺结果及激素治疗后迅速缓解疗效, 诊断为特发性血小板减少性紫癜(ITP). 急性暴发型UC合并ITP临床罕见.     

Determination of cranio-spinal canal compliance distribution by MRI: Methodology and early application in idiopathic intracranial hypertension

Purpose:

To develop a method for derivation of the cranial‐spinal compliance distribution, assess its reliability, and apply to obese female patients with a diagnosis of idiopathic intracranial hypertension (IIH).

Materials and Methods:

Phase contrast‐based measurements of blood and cerebrospinal fluid (CSF) flows to, from, and between the cranial and spinal canal compartments were used with lumped‐parameter modeling to estimate systolic volume and pressure changes from which cranial and spinal compliance indices are obtained. The proposed MRI indices are analogous to pressure volume indices (PVI) currently being measured invasively with infusion‐based techniques. The consistency of the proposed method was assessed using MRI data from seven aged healthy subjects. Measurement reproducibility was assessed using five repeated MR scans from one subject. The method was then applied to compare spinal canal compliance contribution in seven IIH patients and six matched healthy controls.

Results:

In the healthy subjects, as expected, spinal canal contribution was consistently larger than the cranial contribution (average value of 69%). Measurement variability was 8%. In IIH, the spinal canal contribution is significantly smaller than normal controls (60 versus 78%, P < 0.03).

Conclusion:

An MRI‐based method for derivation of compliance indices analogous to PVI has been implemented and applied to healthy subjects. The application of the method to obese IIH patients suggests a spinal canal involvement in the pathophysiology of IIH. J. Magn. Reson. Imaging 2011;. © 2011 Wiley Periodicals, Inc.     

聚丙烯酰胺水凝胶对L929细胞的细胞毒性研究

比较国产聚丙烯酰胺水凝胶（ＦＨ）与进口聚丙烯酰胺水凝胶（ＩＴ）的细胞毒性。用ＭＴＴ法检测Ｌ９２９ 细胞在不同浓度的两种聚丙烯酰胺中的相对增殖率（ＲＧＲ,％）,按通用标准评价材料的细胞毒性。Ｌ９２９ 细胞在１２．５ ％ 的ＩＴ中２、４、６ 天时的ＲＧＲ分别为９６ ．６ 、９１．２ 和９０．９ ,在２５％ 的ＩＴ中为９３．１、８３ ．１ 和８９．３,在５０ ％ 的ＩＴ中为８９．７、８５．０ 和９０．９;而在１２ ．５ ％ 的ＦＨ中２、４、６ 天时的ＲＧＲ分别在９８．３、８４．４ 和９１．３ ,在２５ ％ 的ＦＨ中为９４．８、８６．９ 和８９．３ ,在５０ ％的ＦＨ中为８９．７ 、８７ ．５ 和９６ ．４;以上各组材料毒性级别均为“１”。ＦＨ 和ＩＴ都属于无细胞毒性的材料。     

Binding of the [125I]3β-(Iodophenyl)tropan-2β-carboxylic acid isopropyl ester to the dopamine transporter at a physiologically relevant temperature: Mutually exclusive binding and different ionic requirements for various uptake blockers and substrates

The present study describes the binding of cocaine analog [¹²⁵I]3β-(iodophenyl)tropan-2β-carboxylic acid isopropyl ester ([¹²⁵I]RTI-121), a highly selective ligand for the dopamine transporter (DAT), to rat striatal synaptosomal membranes at 37°C. Saturation analysis of [¹²⁵I]RTI-121 binding revealed a single binding site with similar affinity for RTI-121 at both 50 and 134 mM NaCl. However, the density of binding sites was reduced at 134 mM NaCl. Various uptake blockers and substrates of the DAT monophasically inhibited the specific binding of [¹²⁵I]RTI-121. Increasing the NaCl concentration from 50 mM to 134 mM enhanced the affinity of the substrate dopamine and amphetamine for the DAT, without affecting that of the uptake blockers. At 134 mM NaCl, the copresence of GBR12935, BTCP, cocaine, amphetamine, or dopamine decreased the affinity of RTI-121 to the extent predicted by a model in which the binding of all compounds is mutually exclusive. This, along with a different NaCl sensitivity for blockers and substrates, suggests that the two categories of compounds recognize nonidentical but overlapping binding domains on the DAT. In contrast, the mutually exclusive binding with similar NaCl sensitivity for RTI-121 and the other uptake blockers tested here suggests the involvement of common binding domains in the recognition of these blockers. Synapse 25:155–162, 1997. © 1997 Wiley-Liss, Inc.     

Longitudinal performance of self-collected human papilloma virus testing and other mainstream strategies in detecting prevalent and incident cervical precancer: a 15-year cohort study in china

Background

Comprehensive long-term evaluation for self-collected human papilloma virus (HPV) testing is not well established. We aimed to assess its predictive capacity from a long-term perspective compared with other cervical cancer mainstream screening methods.

纳米级散射颗粒混浊介质的Mueller矩阵测量

Mueller矩阵是公认的能很好地表述介质偏振特性的一种方法,对生物组织散射特性的Mueller矩阵的研究已成为国际上组织光学的热点之一。在实验上对纳米级ZnO散射颗粒酒精溶液Mueller矩阵各元素的空间分布图样进行了测量,发现:粒径越小,某些矩阵元图样有收缩趋势,结果显示Mueller矩阵与介质特性之一的散射颗粒半径有关。预计通过对生物组织等混浊介质后向散射Mueller矩阵的研究,在医疗诊断方面(如肿瘤细胞的检测)有潜在的应用价值。     

The burden of cervical cancer in China: synthesis of the evidence

The burden of cervical cancer in China has not been characterized in detail. We reviewed cervical cancer data from national mortality surveys and registries, and conducted a meta-analysis to estimate the prevalence of high-grade lesions (HSIL) and high-risk human papillomavirus (HR-HPV) infections in rural Shanxi Province. We found that a national survey in the 1970s estimated age-standardized cervical cancer mortality rates as ~15 and ~83/100,000 women nationally and in Xiangyuan, Shanxi; but the latest survey (2004-2005) found much lower rates of ~3 and ~7/100,000, respectively. IARC registries record age-standardized cervical cancer incidence in China as <5/100,000 (1998-2002); but the five registry sites cover <2% of the population, and the gross domestic product per capita at each of the registry sites is higher than China's average (by a factor ranging from 1.3 to 3.9). The pooled estimate of the prevalence of HSIL and HR-HPV in women aged 30-54 years in Shanxi was 3.7%(95%CI:2.7-4.8%) and 17.2%(95%CI:13.1-21.3%), respectively. Based on a feasible range informed by the incidence data for China and other unscreened populations, the predicted indicative annual number of new cervical cancer cases nationally, in the absence of any intervention, ranges from ~27,000 to 130,000 (2010) to 42,000 to 187,000 (2050). In conclusion, recent data suggest comparatively low rates of cervical cancer incidence in China, which may be partly explained by the location of registry sites in higher socioeconomic status areas. However, the evidence is consistent with considerable heterogeneity within China, with a higher disease burden in some rural areas such as Shanxi. Therefore, the lower reported rates of cervical cancer in China should be interpreted cautiously.