巩膜外冷凝联合巩膜扣带术治疗视网膜脱离

目的 研究巩膜外冷凝联合巩膜扣带术治疗视网膜脱离的疗效。方法 在视网膜裂孔对应的巩膜外冷凝、放出视网膜下液、巩外加压。结果 随诊1mo以上,35眼中,32眼达到解剖复位,成功率为91．43％。结论 巩膜外冷凝联合巩膜扣带术治疗视网膜脱离,手术操作简单,手术反应轻,疗效满意,是一理想的手术方法。     

肢体负压对周围动脉闭塞性病变犬皮肤SP免疫反应阳性神经纤维影响

目的　观察肢体负压对周围动脉闭塞性病变犬皮肤中 SP免疫反应阳性神经纤维的影响 .方法　犬 17只 ,随机分治疗组 10只、非治疗组 5只和正常对照组 2只 ,治疗组和非治疗组均将动物制作左后肢缺血模型 ,治疗组在模型制作后 14d,开始行患肢负压治疗 10 d(15 min·次 - 1 ) ;非治疗组不做负压治疗 ;正常对照组不行缺血模型制作及负压治疗 . 3组均行左后肢趾皮肤免疫组化染色 ,检测 SP免疫反应阳性纤维 .结果　非治疗组皮肤中 SP免疫反应阳性神经纤维均较正常对照组明显增多 (P<0 .0 1) ,治疗组较非治疗组减少(P<0 .0 1) ,但仍较正常对照组增多 .结论　肢体负压疗法可促进皮肤感觉神经纤维中 SP的释放     

术中应用丝裂霉素C预防翼状胬肉术后复发的疗效观察

OBJECTIVE: To explore the feasibility of intraoperative application of mitomycin C (MMC) in pterygium and observe the ultrastructure change of pterygium with or without intraoperative application of MMC. METHODS: Sixty two eyes of 57 patients with primary pterygium were randomly divided into MMC group and control group, 29 patients (32 eyes) in MMC group underwent transposition with intraoperative application of MMC (0.4 mg.ml-1 for 1 minute), whereas 28 patients (30 eyes) in control group underwent transposition. The recurrence rate, complications and side effects were observed and compared between the two groups. The ultrastructure of pterygium with or without intraoperative application of MMC was studied with transmission electron microscopy. The mean follow-up was(7.6 +/- 3.4) months (1-13 minths). RESULT: Eleven eyes of the 30 eyes in control group (36.%) showed recurrence, whereas only 2 of 32 eyes (6.3%) in MMC group showed recurrence (P < 0.005). No severe complication and side effect appeared during the follow-up. Most of fibroblasts in pterygium that underwent intraoperative MMC occurred the change of rough endoplasmic reticulum (RER) dilatation. CONCLUSION: Intraoperative MMC appears to be an effective treatment in preventing recurrence of primary pterygium. The fibroblasts in pterygium have RER dilatation after application of MMC for 1 minute during the operation.     

椎基底动脉短暂缺血性眩晕患者脑血流量与脑干听力诱发电位的关系研究

为探讨椎基底动脉短暂缺血性眩晕 (VBTIV)患者的脑干听力诱发电位 (BAEP)与脑血流量 (CBF)和病理改变的关系 ,对 6 5例 VBTIV患者 (患者组 )作磁共振血管成像 (MRA)、经颅多普勒 (TCD)和 BAEP检查 ,对 2 6例对照组患者作 MRA和 TCD检查。结果 :MRA显示患者组 5 0例血管病理改变明显 ,1 5例改变不明显或无改变 ;对照组脑血流量为 1 1 2 .3～ 2 78.9ml/ min(2 s) ,较患者组 48.0～ 2 6 2 .0 m l/ min高 (P<0 .0 1 ) ,患者组 1 5例脑血流量下降 ,5 0例脑血流量正常 ;患者组 BAEP异常 47例 (72 .3% ) ,其中 ,1 5例脑血流量下降者 ,1 4例(93.3% ) BAEP异常 ,比 5 0例脑血流量正常者中的 33例 (6 6 % ) BAEP异常高 (P<0 .0 5 ) ;5 0例血管病理改变明显者 ,BAEP异常 40例 (80 % ) ,比 1 5例血管病理改变轻或无改变者中的 7例 (43.3% ) BAEP异常高 (P<0 .0 5 ) ,说明BAEP与 CBF和血管病理改变密切相关     

Changes of type I collagen in the lungs of rats with hypoxic pulmonary hypertension]

The changes of type I collagen in the lung tissues of rats with hypoxic pulmonary hypertension (HPH) and its relationship to the mean pulmonary arterial pressure (mPAP) were investigated. The blood dynamic indexes of pulmonary circulation were measured by using Swan-Ganz. The distribution of type I collagen in pulmonary arteries, bronchi and plumonary interstitial was observed by using streptavidin peroxidase method (SP). The morphologic changes of small pulmonary arteries and the level of type I collagen were determined by image pattern analysis technique and gray scale scanning, respectively. The results showed, compared with the controls, mPAP of the hypoxic rats elevated (from 2.12 +/- 0.25 to 3.95 +/- 0.43 kPa, P < 0.01), their small pulmonary arteries, walls thickened (MT% from 16.35 +/- 2.64 to 35.83 +/- 3.55, MA% from 26.83 +/- 3.40 to 59.68 +/- 4.90, P < 0.01) and lumen narrowed. The distribution of type I collagen was mainly in the external layer of pulmonary arterial walls; the positive degrees of gray scale scanning increased and had a positive linear relationship to the mPAP and MT%. These findings suggest that the increase of type I collagen contents in pulmonary arteries is closely related to the development of HPH.     

Transcriptome analysis of Armillaria gallica 012 m in response to auxin

Gastrodia elata is an achlorophyllous and fully mycoheterotrophic orchid which obtains carbon and other nutrients from Armillaria species in its life cycle. Many researchers suggested that plant hormones, as signing molecules, play a central role in the plant–fungi interaction. In the process of Armillaria gallica 012 m cultivation, both exogenous indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) distinctly stimulated the growth of mycelia in solid media. The differential expression genes (DEGs) of A. gallica 012 m with IAA versus blank control (BK) and IBA versus BK were investigated. The results showed that more than 80% of DEGs of the IAA group were coincident with the DEGs of the IBA group, and more than half of upregulated DEGs and most of the downregulated DEGs of the IAA group coincided with those DEGs of the IBA group. Above research implied that A. gallica 012 m could perceive IAA and IBA, and possess similar responses and signaling pathways to IAA and IBA. The overlapping differential genes of the IAA group and IBA group were analyzed by GO term, and the results showed that several DEGs identified were related to biological processes including positive regulation of the biological process and biological process. The downregulated NmrA-like and FKBP_C genes might be benefit to the growth of mycelia. Those results can explain that exiguous IAA and IBA improved the growth of A. gallica to some extent. We speculate that IAA and IBA are signaling molecules, and regulate the expression of growth-related genes of A. gallica 012 m by the same signaling pathway.     

The blocking effects of glycyrrhize uralensis and chelidonium majus on mutagenesis induced by Aflatoxin Bl

Glycyrrhiza Uralensis (GU) and Chelidonium Majus (CM) (25, 50, 100, 200 mg/plate) have obvious blocking effects onSalmonella Typhimurium strains (TA97, TA98, TA100, TA102) mutagenesis induced by aflatoxin Bl (AFB1). The blocking rates of GU and CM are more dependent on the doses. A compound of GU and CM, called Wei Lingsu (WLS), could impede chromosomol aberrations and sister chromatid exchanges (SCE) of human peripheral lymphocyte induced by AFB1, at the concentrations of 1–50 mg/ml, the blocking rates are 52.94%–88.24% and 15. 50%–38.23% saperatly. WLS could also impede the inhibition of AFB1 to lymphocyte mitosis.