全胃切除术后Lahey法及Lawrence法消化道重建术比较分析

d:\\PDF\\.pdf目的 :探讨Lahey法及Lawrence法消化道重建术对胃癌全胃切除术后患者营养状态及消化道症状的影响。 方法 :回顾性分析146例胃癌行全胃切除术的患者,其中以Lahey法重建者83例,以Lawrence法重建者63例,比较术后6个月时患者体重变化、营养状况以及消化道症状。 结果 :术后6个月Lawrence组体重下降程度显著低于Lahey组;血浆总蛋白、白蛋白水平变化,Lawrence组分别升高,而Lahey组分别降低。血红蛋白水平Lawrence组下降程度低于Lahey组,但无显著性差异。消化道症状,进食量、进食次数无显著性差异;呕吐者Lawrence组显著低于Lahey组。 结论 :Lawrence法消化道重建术是胃癌全胃切除术后较为合理的一种消化道重建方式。     

Salidroside alleviates ulcerative colitis via inhibiting macrophage pyroptosis and repairing the dysbacteriosis-associated Th17/Treg imbalance

Ulcerative colitis (UC) is a chronic inflammatory bowel disease characterized by flora disequilibrium and mucosal immunity disorder. Here, we report that salidroside effectively restricts experimental colitis from two aspects of intestinal macrophage pyroptosis and dysbacteriosis-derived colonic Th17/Treg imbalance. In innate immunity, the upregulated TREM1 and pyroptosis-related proteins in inflamed colons were inhibited by salidroside administration and further experiments in vitro showed that salidroside suppressed LPS/ATP-induced bone marrow-derived macrophages (BMDMs) pyroptosis evident by the decline of LDH and IL-1β release as well as the protein level of NLRP3, caspase-1, and GSDMD p30. Moreover, the TREM1 inhibitor weakened the effect of salidroside on BMDMs pyroptosis, whereas salidroside still could downregulate TREM1 when NLRP3 was inhibited. In adaptive immunity, salidroside improved the gut microflora diversity and Th17/Treg ratio in DSS-induced mice, especially promoting the abundance of Firmicutes. Clearance of the gut flora blocked the benefit of salidroside on colonic inflammation and Th17/Treg adaptive immunity, but transplanting salidroside-treated foecal bacterium into flora-depleted wild mice reproduced the resistance of salidroside to gut inflammation. Taken together, our data demonstrated that salidroside protected experimental colitis via skewing macrophage pyroptosis and Th17/Treg balance, indicating its potential effect on UC and other immune disorders.     

云南地区晚期非小细胞肺癌患者的基因突变及其临床意义

目的:探讨云南地区晚期非小细胞肺癌(non-small cell lung cancer,NSCLC)患者外周血中肺癌相关驱动基因突变及其与临床病理特征的关系.方法:收集2019年1月至2019年12月云南省肿瘤医院分子诊断中心检测的304例Ⅳ期NSCLC患者外周血,用二代测序(next generation sequ...