胸腺因子D对实验性大鼠肝纤维化防治作用的研究

目的　研究胸腺因子D(TFD)的体内抗肝纤维化作用。方法　用四氯化碳 (CCL4 )复制大鼠肝纤维化模型 ;造模开始或造模 30d后予TFD ;实验结束后分别测定肝功能、纤维化指标 (Ⅲ型前胶原、透明质酸和层粘连素 )、肝组织中谷胱甘肽、超氧化物岐化酶及丙二醛含量 ,并作肝病理检查。结果　TFD可显著减轻CCl4 肝纤维化程度 ,能明显降低血清Ⅲ型前胶原、透明质酸、层粘连素和肝组织中丙二醛含量 ;显著提高谷胱甘肽和超氧化物岐化酶活性 ,改善肝功能 ,组织学检查亦显示具有抗肝纤维化作用。结论　TFD在体内具有抗肝纤维化的作用 ,可望用于肝纤维化的防治。     

CD3AK细胞冻融提取液的抗肿瘤活性研究

为研究ＣＤ３ＡＫ细胞冻融提取液的抗肿瘤作用,作者采集正常人外周血分离单个核细胞（ＰＢＭＣ）,先用抗ＣＤ３的单克隆抗体（ＣＤ３ＭｃＡｂ）的ｒＩＬ－２诱导和激活,使之成为ＣＤ３ＭｃＡｂ激活的杀伤细胞（ＣＤ３ＡＫ）,再制备ＣＤ３ＡＫ的冻融提取液,然后进行体内,外抗肿瘤实验。结果;低剂量的ＣＤ３ＭｃＡｂ和ｒＩＬ－２能明显诱导ＰＢＭＣ的活化和增殖;激活后的ＣＤ３ＡＫ细胞冻融提取液可抑制小鼠肝癌实体瘤细胞Ｈ２     

冠状动脉架桥术麻醉及围手术期处理

目的 :介绍 1997年 4月至 1999年 10月我院 180例冠脉架桥手术 (CABG)患者麻醉特点及处理原则。方法 :采用小剂量咪唑安定 ,阈下值氯胺酮 ,常规剂量芬太尼诱导 ,异氟醚、异丙酚持续镇静的麻醉方法 ,分析180例接受CABG手术的患者的临床资料及麻醉用药情况和术中血流动力学状况。结果 :本组麻醉诱导平稳 ,循环稳定 ,体外循环时间平均 115min ,升主动脉阻断时间平均 5 5min。围术期死亡率 0 6 % (1例死于鱼精蛋白过敏 )。患者术后清醒时间 46h ,拔管时间平均 14h。结论 :即使是在严重冠心病患者 ,采用多种小剂量镇静药及大剂量芬太尼诱导、维持的麻醉方法 ,能获得平稳的麻醉诱导和维持以及稳定的血流动力学状态 ,从而有效防止围手术期低血压和心律失常造成的心肌缺血和围手术期心肌梗死的发生。     

Lead-induced blockade of kainate-sensitive receptor channels

The effects of bivalent lead on ion channels activated by kainate and -amino-3-hydroxy-5-methyl-4-isoxazolpropionate (AMPA) were studied using Xenopus oocytes microinjected with mRNA from rat brain. Lead reduced kainate-induced membrane currents in a reversible and dose-dependent manner, without affecting membrane currents induced by AMPA. Lead decreased the kainate currents with a concentration of 0.1 mol/l to 0.93 ± 0.01 and with a concentration of 100 mol/l to 0.41 ± 0.04 of the control values. The blocking effect of lead on kainate responses was voltage dependent. The inhibition was strongest at - 90 mV to - 70 mV and became weaker at more positive membrane potentials. The effect of lead on the kainate-induced membrane currents remained unchanged when the concentration of kainate was increased. Hence lead probably represents a noncompetitive channel-blocking agent for non-N-methyl-d-aspartate (NMDA) receptor channels activated by kainate.     

The use of the STRs HUMTH01, HUMVWA31/A,HUMF13A1, HUMFES/FPS,HUMLPL in forensic application: Validation studies and population data for Galicia (NW Spain)

The 5 tetranucleotide short tandem repeats, HUMTHOI, HUMVWA31/A, HUMF13A1, HUMFES/FPS and HUMLPL were studied using different electrophoretic methods and PCR amplification conditions in order to optimize the typing conditions. A genetic population study in the population of Galicia was carried out and the allele and genotype frequencies are given. Compliance with the Hardy-Weinberg equilibrium was tested using different statistical parameters, with clear advantages resulting in favor of using the exact test (Guo-Thompson method) instead of conventional chi-square methods. Some statistical parameters of forensic interest (PD, CE, h) were also calculated. There were no mutations found in a total of 73 paternal meioses and 101 maternal meioses. Abnormal electrophoretic mobility was found in the AT-rich STR HUMF13AI under non-denaturing conditions and, therefore, the use of denaturing conditions is absolutely necessary. No "stutter" bands were found, although double peaks in the HUMFES/FPS system were observed in some samples. The advantage of using automated sequencers with fluorescent technology is also reported.     

北京市西城区1989～1997年预防接种副反应分析

为分析发生预防接种副反应的原因,以控制和降低副反应的发生率,对１９８９～１９９７ 年北京市西城区预防接种副反应的监测资料做了分析。１９８９～１９９７ 年北京市西城区预防接种副反应发生率为３８－４９／１０ 万。其中１９９４ 年副反应发生率最低,为０－８４／１０ 万;１９９７ 年副反应发生率最高,为３１６－５７／１０ 万。副反应发生年龄以６ 岁最多,占总反应的７２－７０ ％ ;其次为＜ １ 岁,占９－３９％ 。９０－０９ ％ 的副反应发生在接种后≤１ 天。８８－１７％ 的副反应与接种≥２ 次同种疫苗有关。一般反应、加重反应、异常反应分别占总反应的２－０９％ 、７０－６１％ 、２７－３０％ 。其中１５７ 例异常反应中过敏反应占５９－２４％ ,非特异性反应占４０－７６％ 。     

大鼠肝细胞线粒体三维结构的观察与重建

为深入研究大鼠肝细胞线粒体形态特征,本研究以钻石刀连续超薄切片,通过电子显微观察大鼠肝细胞线粒体的三维结构,对特殊形态的线粒体进行三维结构重组。结果表明：球形,椭球形,盘状及杆状等小型线粒体约占线粒体总数的５３．８％,而分枝形及各种不规则形的线粒体约占４１．６％。     

Chronic and acute compressive spinal cord lesions in dogs due to intervertebral disc herniation are associated with elevation in lumbar cerebrospinal fluid glutamate concentration

Acute injury to the central nervous system initiates a series of biochemical events that cause secondary tissue damage. The accumulation of excessive concentrations of glutamate in the extracellular space causes excitotoxic damage, and is incriminated as a mediator of this secondary tissue damage. The aim of this study was to measure the concentration of glutamate in cerebrospinal fluid (CSF) obtained from the cerebellomedullary cistern and lumbar subarachnoid space in dogs with acute and chronic compressive injuries of the cervical and thoracolumbar spinal cord, and to correlate the glutamate concentration with injury severity. The results demonstrate that focal injuries of the spinal cord do not affect the glutamate concentration in CSF taken from the cerebellomedullary cistern. However, dogs with severe, acute thoracolumbar disc herniations have two- to 10-fold increases in glutamate concentration in their lumbar CSF at intervals of >12 h after injury. Moreover, the severity of their clinical signs is directly related to the glutamate concentration. Dogs with chronic compressive thoracolumbar lesions have a two-fold elevation of CSF glutamate concentration, suggesting that excitotoxicity may also be a component of chronic spinal cord compression.