Circ_SAR1A regulates the malignant behavior of lung cancer cells via the miR‐21‐5p/TXNIP axis

BackgroundLung cancer is one of the most common malignancies globally and a significant component of cancer‐related deaths. The lack of early diagnosis accounts for detecting approximately 75% of cancer patients at an intermediate to an advanced stage, with a low 5‐year survival rate. Therefore, a more comprehensive understanding of the molecular mechanisms of lung cancer development is necessary to find reliable and effective therapeutic and diagnostic biomarkers.Methodscirc_SAR1A, miR‐21‐5p, and TXNIP in lung cancer tissues, animal xenografts, and cell lines were validated by qRT‐PCR and western blotting analyses. RNase R digestion and nuclear/cytoplasm fractionation experiments were utilized to determine the stability and localization of circ_SAR1A in lung cancer cells. The binding between miR‐21‐5p and circ_SAR1A or TXNIP was confirmed by luciferase reporter, RNA pull‐down, Spearman''s correlation, and rescue assays. CCK‐8, colony formation, flow cytometry, Transwell, and western blotting were utilized to illustrate the malignant behavior of lung cancer cells.Resultscirc_SAR1A and TXNIP were down‐regulated while miR‐21‐5p was up‐regulated in lung cancer samples and cells. circ_SAR1A was located predominantly in the cytoplasm; it inhibited lung cancer growth in vitro and in vivo by sponging to miR‐21‐5p. miR‐21‐5p silencing suppressed lung cancer malignancy by targeting TXNIP.Conclusionscirc_SAR1A is a critical negative regulator of lung carcinogenesis. circ_SAR1A/miR‐21‐5p/TXNIP attenuation inhibited lung cancer progression, presenting an ideal diagnostic and a potential therapeutic target.     

Effect of L-Glutamine on Chylomicron Formation and Fat-Induced Activation of Intestinal Mucosal Mast Cells in Sprague-Dawley Rats

Glutamine (Gln) is required for intestinal mucosal homeostasis, and it can promote triglyceride absorption. The intestinal mucosal mast cells (MMCs) are activated during fat absorption. This study investigated the potential role of Gln on fat absorption-induced activation of MMCs in rats. Lymph fistula rats (n = 24) were studied after an overnight recovery with the infusion of saline only, saline plus 85 mM L-glutamine (L-Gln) or 85 mM D-glutamine (D-Gln), respectively. On the test day, rats (n = 8/group) were given an intraduodenal bolus of 20% Intralipid contained either saline only (vehicle group), 85 mM L-Gln (L-Gln group), or 85 mM D-Gln (D-Gln group). Lymph was collected hourly for up to 6 h for analyses. The results showed that intestinal lymph from rats given L-Gln had increased levels of apolipoprotein B (ApoB) and A-I (ApoA-I), concomitant with an increased spectrum of smaller chylomicron particles. Unexpectedly, L-Gln also increased levels of rat mucosal mast cell protease II (RMCPII), as well as histamine and prostaglandin D₂ (PGD₂) in response to dietary lipid. However, these effects were not observed in rats treated with 85 mM of the stereoisomer D-Gln. Our results showed that L-glutamine could specifically activate MMCs to degranulate and release MMC mediators to the lymph during fat absorption. This observation is potentially important clinically since L-glutamine is often used to promote gut health and repair leaky gut.     

外企员工中患脂肪肝、高血糖、高血压、高脂血症情况的分析

目的了解外企员工中高血压、脂肪肝、血脂、高血糖的情况,为做好预防保健提供参考。方法回顾性统计天津国际旅行卫生保健中心2005-2006年外资企业员工体检结果并进行分析。结果四种疾病检出率男性均高于女性。结论经统计学分析,高脂肪肝、高脂血症性别之间存在显著性差异,而高血压、高血糖无显著性差异。     

乙型肝炎病毒基因分型及分型方法的研究进展

中国是乙型肝炎的高度流行区,全国人群HBV感染率平均约60%,流行病学调查结果显示人群中的HBsAg的携带率已经从10%上升至10.12%,而这些病毒携带者中约有25%的人将发展成各种类型的     

Cyanidin-3-O-Glucoside Ameliorates Palmitic-Acid-Induced Pancreatic Beta Cell Dysfunction by Modulating CHOP-Mediated Endoplasmic Reticulum Stress Pathways

Cyanidin-3-O-glucoside (C3G) is a natural colorant with anti-diabetic properties, while its underlying mechanisms remain far from clear. Here, we investigated the protective role of C3G on palmitic acid (PA)-induced pancreatic beta cell dysfunction and further decipher its possible molecular mechanisms. Both primary isolated mouse islets and the INS-1E cell were used, and treated with a mixture of PA (0.5 mM) and C3G (12.5 µM, 25 µM, 50 µM) for different durations (12, 24, 48 h). We found that C3G could dose-dependently ameliorate beta cell secretory function and further alleviate cell apoptosis. Mechanistically, the primary role of the PKR-like ER kinase (PERK) endoplasmic reticulum (ER) stress pathway was detected by RNA sequencing, and the PERK-pathway-related protein expression, especially the pro-apoptotic marker C/EBP homologous protein (CHOP) expression, was significantly downregulated by C3G treatment. The critical role of CHOP in mediating the protective effect of C3G was further validated by small interfering RNA. Conclusively, C3G could ameliorate PA-induced pancreatic beta cell dysfunction targeting the CHOP-related ER stress pathway, which might be used as a nutritional intervention for the preservation of beta cell dysfunction in type 2 diabetes mellitus.     

终末期肾脏病患者外周血Th17、Treg细胞表达及其平衡关系的研究

目的 探讨终末期肾脏病患者外周血CD4+T淋巴细胞、Th17细胞及CD4+CD5+CD127-调节T细胞(Treg)变化及意义.方法 采用流式细胞术检测21例尿毒症患者(尿毒症组)、21例肾移植受者(移植组)和21例健康志愿者(对照组)外周血中Th17细胞和Treg细胞占CD4+T细胞的比例.结果 尿毒症组和移植组的Th17/CD4+T细胞的比例明显低于对照组(P＜0.05,P＜0.01);尿毒症组和移植组的Treg/CD4+T细胞的比例明显低于对照组(P ＜0.01,P＜0.01);尿毒症组的Th17/Treg细胞的比值明显高于对照组(P＜0.05,P＜ 0.01).结论 尿毒症患者外周血中存在Th 17/Treg失衡,Th 17/Treg失衡可能参与了患者的免疫异常从而导致尿毒症的发生和发展.     

精神分裂症患者自我面孔识别与共情的相关性

目的 探讨精神分裂症患者自我面孔识别能力及其与共情能力的关系.方法 采用自我面孔识别任务(SFRT)对62例精神分裂症患者进行测试,与54例健康对照者作比较,同时作人际反应指针量表(IRI-C)评估.结果 患者组SFRT平均反应时[(2188±1138) ms]长于对照组[(1152±326) ms,P＜0.01],正确率[(80±16)％]低于对照组[(88±6)％,P＜0.01].患者组IRI-C总分、PT分、FS分、EC因子分[分别为(44.82±10.50)分,(8.98±3.56)分,(11.87 ±4.38)分,(14.73 ±4.00)分]均低于对照组[分别为(49.85±10.28)分,(10.78 ±3.86)分,(14.98±6.12)分,(17.39 ±4.56)分],PD因子分[(9.37±5.12)分]高于对照组[(6.52±3.89)分],差异具有统计学意义(P＜0.05 ～0.01).患者组SFRT正确率与IRI-C的FS因子分呈正相关(r=0.322,P＜0.05),平均反应时与PD因子分呈正相关(r=0.303,P＜0.05);对照组SFRT正确率与IRI-C的FS因子分呈正相关(r=0.360,P＜0.01).结论 精神分裂症患者存在自我面孔识别及共情能力的缺损,自我面孔识别能力与共情能力间存在某种关联.     

甲减肾损害大鼠的以方测证研究

目的:通过观察不同中药复方对甲状腺功能减退症(简称甲减)肾损害大鼠肾脏功能及形态的影响,探求甲减肾损害大鼠的主要证候属性。方法:将Wistar大鼠随机分为正常组和实验组,实验组建立甲减肾损害大鼠模型后,随机分为模型组、补中益气汤组、逍遥散组和金匮肾气丸组,并施以相应处理因素。90天后,测定血清甲状腺激素(TT3、TT4)、血清促甲状腺激素(TSH)、24h尿白蛋白、血清肌酐(Scr)和血清尿素氮(BUN),观察肾脏结构。结果:①24h尿白蛋白、Scr和BUN:补中益气汤组高于正常组(P<0.05),低于模型组(P<0.01),低于逍遥散组和金匮肾气丸组(P<0.05);逍遥散组和金匮肾气丸组低于模型组(P<0.05)。②模型组肾小球体积增大,系膜区增宽、系膜细胞及系膜基质增生;3个治疗组病理改变均有不同程度减轻,且补中益气汤组改善显著。结论:在甲减肾损害机体内,补中益气汤可较好地改善肾脏功能及形态,从而推测脾气虚弱在甲减肾损害发病中起重要作用。     

优质护理在门诊眼科护理中的应用观察

目的：探讨优质护理模式在门诊眼科护理中的应用效果及意义。方法将在我院门诊眼科接受治疗的100例患者随机分为观察组和对照组,观察组在常规治疗过程中进行优质护理干预,对照组实施一般眼科常规护理,采用问卷形式对2组患者的生活质量及满意度进行比较分析。结果观察组患者生活质量提升明显,心理状态稳定,能积极配合治疗,患者满意率为100%;对照组患者满意率为88%,2组比较差异有统计学意义（P<0.01）。结论优质护理在门诊眼科中的应用,有助于提升患者生活质量,保持心理状态稳定,提高对医疗行为的满意度,值得推广。