急性冠脉综合征中妊娠相关蛋白-A与斑块不稳定性

目的探讨妊娠相关蛋白-A(PAPP-A)在急性冠脉综合征(ACS)中的变化及其与斑块不稳定性的关系。方法26例冠脉正常者作为对照组,稳定性心绞痛(SA)组22例,ACS组40例,包括不稳定性心绞痛(UA)20例、急性心肌梗死(AMI)20例。定量检测外周血PAPP-A、C-反应蛋白(CRP)、肌酸激酶MB同工酶(CK-MB)。对所有冠脉造影显示狭窄≥30%的病变进行影像学分析。结果ACS中PAPP-A含量(13·19±4·04)mIU/L明显高于SA(5·47±3·00)mIU/L(P<0·01)及对照组(5·09±2·19)mIU/L(P<0·01);并且与CRP(r=0·62P<0·01)、复杂性狭窄的数量(r=0·45P<0·05)有显著的正相关。结论PAPP-A在ACS患者外周血中明显升高,可能与斑块的不稳定性有关,对ACS的危险度分层可能具有一定的价值。     

Functional aspects of mouse islets transplanted to the kidney

Diabetologia - To test whether the transplantation of pancreatic islets affects their basic functions, collagenaseisolated mouse islets were inserted under the left renal capsule of recipient...     

Reduction in LBNP tolerance following prolonged endurance exercise training.

Eight young men underwent an 8-month endurance exercise training program. Prior to and following the training program, the subjects' maximal oxygen uptake (VO2max), total blood volume (TBV) and plasma volume (PV), tolerance to lower body negative pressure (LBNP) assessed by the cumulative stress index (CSI) to presyncope, and their hemodynamic responses to 0 to -45 torr LBNP was determined. Hemodynamic measures included rebreathe carbon dioxide cardiac output (Qc), heart rate (HR), directly measured arterial blood pressures (ABP), and strain gauge determination of forearm blood flow (FBF) and leg volume changes (delta LgV). Calculated values of stroke volume (SV), forearm, vascular resistance (FVR), and peripheral vascular resistance (PVR) were made. Following training, each subject had an increased VO2max (mean = +27.4%, P < 0.001), TBV (mean = +15.8%, P < 0.02), and PV (mean = +16.5%, P < 0.02) and each subject had a decreased tolerance to LBNP (mean CSI = -24%, P < 0.001). Stepwise linear regression identified that the major factors to significantly predict the decreased CSI pre- to post-training were a reduced response of PVR to LBNP from -15 to -45 torr (Model R2 = 0.853), the delta TBV (model R2 = 0.981), and the greater post-training reduction in SBP to LBNP of 0 to -45 torr (model R2 = 1.0). These data suggest that physiologic adaptations associated with the increased VO2max and TBV resulting from a prolonged endurance exercise training program can alter the reflex control of vasomotion and cardiac output during LBNP and reduce the LBNP tolerance.     

乙酰胆碱参与皮层下行调制疼痛的作用

<正> 我们实验室的工作曾报导,刺激猫的皮层SⅡ区、SⅠ区或十字沟前区可抑制丘脑髓板内核群神经元的伤害性反应及针刺镇痛效应。相继刺激大鼠的皮层SmⅠ区也获     

培养小鼠骨髓细胞半同系移植研究的补充材料

用体外培养不同时间的C57BL/6雄性小鼠骨髓细胞移植到受致死剂量γ线照射的DBF_1雌性小鼠,记录受照射小鼠30天和60天的活存率,并分析造血和免疫功能的主要指标.对部分长期活存小鼠(60～110天)的骨髓细胞和淋巴细胞的染色体核型作细致观察,了解移植进去的造血细胞是否已被植入,结果表明移植培养骨髓细胞的受照射小鼠活存率远较照射对照组为高,造血与免疫功能明显地重建,这无疑是由于移植骨髓在照射动物中形成稳定的辐射嵌合体所致.     

海岛纤恙螨经卵传递恙虫病立克次体的研究

1985年8月～1986年11月,作者以我国发现的恙螨新种海岛纤恙螨子代幼虫656只,分为21组,叮咬健康小鼠,从子1代到子4代幼虫叮咬过的小鼠中,连续分离出7株恙虫病立克次体,证明海岛纤恙螨能够经卵传递恙虫病立克次体至少4代.同时在该螨的卵、幼虫、若虫、成虫组织细胞内发现有恙虫病立克次体寄生,并发现有分裂增殖形态的立克次体,证明海岛纤恙螨各变态期内均保有恙虫病立克次体,这些立克次体能在恙螨体内大量增殖,连续传代.此项研究结果,对于确认海岛纤恙螨为浙江沿海地区恙虫病的传染媒介和宿主,提供了重要的科学依据.     

骨骼肌最大收缩力与最适初长度及其参数的研究

本实验以免载体股直肌为实验模型,研究了该肌在一定脉冲电流刺激下其初长度与收缩力、肌张力及肌力变化的相互关系。同时测定了正常生理状态下兔伸屈膝时股直肌拉长度的变化。结果表明:兔股直肌延长在1.0cm内时,肌力随初长度的延长而增大,超过1.0cm时增大已不明显(p<0.0 1),1.0cm与股直肌的生理最大延长度1.1cm相似。说明兔股直肌的初长度变化不应超过生理最大延长度。而人的骨骼肌与哺乳动物的骨骼肌没有本质的区别,因此,人骨骼肌的初长度变化也不应超过生理最大延长度。肌腱转位后的活动范围应在原位的生理活动范围内,才能发挥最佳收缩效应。     

降钙素基因相关肽对犬冠脉流量及冠状动脉的作用

本研究通过整体及离体灌流实验观察到重庆冠脉狭窄时，犬冠脉流量（ＣＢＦ），平均动脉压（ＭＡＰ）明显减小，而心率（ＨＲ）则增加。狭窄３０ｍｉｎ后由冠状动脉注射降钙素基因相关肽（ＣＧＲＰ）０．３μｇ／ｋｇ后，ＣＢＦ、ＭＡＰ和ＨＲ可恢复正常水平。同时，缺血犬的离体冠状动脉对ＣＧＲＰ的反应也出现改变。大冠脉舒张反应明显降低，而小冠脉的舒张反应与正常相比，无明显改变，这可能与缺血后大冠脉的内皮细胞容易损伤有关。同时也提示:急性心肌缺血时，冠脉流量的减少，主要由于小冠状动脉收缩所致。