ZMW型环氧乙烷灭菌箱对骨移植材料灭菌效果的初步观察

目的 :观察ZMW型环氧乙烷灭菌箱对异体骨材料灭菌效果 ,旨在寻找一种安全、经济的灭菌方法。方法 :清洁条件下取骨 ,制成 0 .5cm× 0 .5cm× 2cm湿润骨材料和干燥骨材料 ,抽取样本 ,用ZMW型环氧乙烷灭菌箱 (15 0L)在不同温度及时间下消毒 ,以环氧乙烷指示胶带是否变色作为灭菌指标 ,样本在消毒前后分别作细菌培养。结果 :不同消毒条件下 ,每批样本均有细菌生长 ,干燥骨材料有菌率高于湿润的骨材料。结论 :ZMW型环氧乙烷灭菌箱不能使异体骨移植材料完全达到灭菌的要求 ,环氧乙烷指示胶带不能作为骨移植材料的灭菌指标 ,干燥可能使细菌的抗环氧乙烷能力增加 ,从而间接的影响了环氧乙烷的消毒效果。     

Studies on the effect of tetrandrine on microtubules. I. Biochemical observation and electron microscopy

Microtubules were purified by using two cycles of assembly and disassembly processes on fresh brain homogenates from 30 guinea pigs. The yield was about 60 mg. The effect of tetrandrine on tubulin was determined by spectrophotometric analysis and electron microscopy. In addition, we used the indirect immunofluorescent method including tubulin antibody to locate the presence of microtubules in 3T3 cells by fluorescence microscopy. The effects of colchicine and P204 were studied for comparison at the same time. The results showed that colchicine can effectively depolymerize microtubules, while tetrandrine showed aggregation, and in a different manner. The shape and structure of microtubules were definitely destroyed by colchicine, but were not affected by P204 which protected against the destructive effect of tetrandrine. This result indicated the safety of using a combination of P204 and tetrandrine in the treatment of silicosis.     

血管紧张素转换酶抑制剂在急性心肌梗塞早期的应用

对２５例急性心肌梗塞（ＡＭＩ）并左心功能不全患者早期应用血管紧张素转换酶抑制剂（ＡＣＥＩ）治疗的对照性临床分析，结果：用小剂量的ＡＣＥＩ治疗对患者心率、血压无显著的影响〔治疗前后心率分别为８９．９０±１０．２２次／分与８９．４２±８．１９次／分，血压分别为１５．３７±３．４６／９．７４±２．３２ｋＰａ（１ｋＰａ＝７．５ｍｍＨｇ）〕和１４．９６±２．６０／９．４６±１．７６ｋＰａ〕；对合并轻、中度泵衰竭患者的疗效显著，但对重度泵衰竭的疗效与对照组比较无显著性差异；治疗组恶性心律失常的发生率较对照组减低１３．３８％，警告性室早的发生率治疗组较对照组减少２６．３９％，但Ⅱ°～Ⅲ°房室传导阻滞的发生率治疗组高于对照组；两组４周病死率比较无显著性差异。作者认为：ＡＣＥＩ治疗ＡＭＩ并轻、中度泵衰竭安全有效；ＡＣＥＩ有一定抗心律失常作用，但应注意传导系统的并发症，使用中应注意各种副作用的发生。     

C_3基因在BXSB小鼠主要脏器中的表达

用同位素标记ｃＤＮＡ探针的分子杂交方法检测了ＢＸＳＢ小鼠肝、肾、脾、胸腺各脏器中补体Ｃ_３ｍＲＮＡ的表达情况，结果表明３～４月龄雄性ＢＸＳＢ小鼠肝、肾、脾各脏器Ｃ_３ｍＲＮＡ的表达量较正常对照鼠显著增加。Ｃ_３基因的过度表达可能参与ＢＸＳＢ小鼠ＳＬＥ多脏器非感染性炎症的发生，同时提示该鼠存在Ｍ系统的大量扩增及活化。     

我国部分地区和民族人血清胆固醇酯脂肪酸组成的研究

本文分析了我国5个地区4个民族共113名成年男性血清胆固醇酯脂肪酸组成及血脂水平。不同地区和民族人血清胆固醇酯脂肪酸组成的基本特点一致。但也有一定差异,这些差异与膳食性质有关。文中讨论了血清胆固醇酯脂肪酸组成与饮食脂肪的质和量及血脂水平的关系,并比较了我国与欧美国家人血清胆固醇酯脂肪酸组成。     

HLA‐DPB1 allelic frequency of the Pumi ethnic group in south‐west China and evolutionary relationship of Pumi with other populations

A sequencing‐based typing of the HLA‐DPB1 gene was carried out in 51 unrelated healthy individuals from the Yunnan Pumi ethnic minority. A total of 18 DPB1 alleles, in which DPB1*0501 (52.0%) and DPB1*0402 (15.7%) greatly predominated, were found, of which alleles DPB1*20011, 2201, 3601, 3701, 3801, 4901, 5001 and 8001 were recorded for the first time in the Chinese population. This may be because the typing methods used in previous genotyping of Chinese populations were of lower sensitivity than that used in our study. A dendrogram constructed by the maximum likelihood method showed that the Pumi ethnic minority belongs to the Asian/Australasian cluster and has the closest relationship to Trobriander, implying an unusual relationship between Australasian and South China populations. However, the Yi ethnic minority, which also comes from the ancient Qiang, did not show a very close relationship with the Pumi. This is probably because the Pumi were historically assimilated by local south‐west China populations.     

Variation at 10 protein coding loci in the mbenzele pygmies from the central african republic and a comparison with microsatellite data

Ten protein coding loci (6‐PGD, A1‐AT, ACP1, CaII, ESD, GC, GPX1, Hbβ, PGM1, and TF) were analyzed in the Mbenzele Pygmies from the Central African Republic. The frequency data were used to calculate the genetic distances between Mbenzele Pygmies and other African groups. In the principal coordinate plot of F_ST genetic distances, the Mbenzele cluster together with other Pygmies of the western cluster, the Biaka from C.A.R., Gielli from Cameroon, and Babinga from Congo. By contrast, they are considerably distanced from other Pygmy groups of the eastern cluster (Twa from Rwanda, Mbuti from Zaire). Genetic distances obtained using protein loci were compared with those based on microsatellite loci. The two distance matrices are insignificantly correlated (r = 0.268; one tail probability = 0.332), and the main difference is in the higher genetic affinity between the Mbenzele and Biaka Pygmies observed at the protein level. Although reasons underlying the discrepancy between inter‐populational variation at protein and DNA loci are not established with certainty, the comparison suggests that the genetic distance between the Mbenzele and Biaka Pygmies at microsatellite loci could have been shaped by genetic drift. Am. J. Hum. Biol. 14:9–14, 2002.© 2002 Wiley‐Liss, Inc.