全文获取类型
收费全文 | 7137篇 |
免费 | 322篇 |
国内免费 | 56篇 |
专业分类
耳鼻咽喉 | 108篇 |
儿科学 | 100篇 |
妇产科学 | 88篇 |
基础医学 | 944篇 |
口腔科学 | 202篇 |
临床医学 | 418篇 |
内科学 | 1936篇 |
皮肤病学 | 127篇 |
神经病学 | 488篇 |
特种医学 | 164篇 |
外科学 | 1257篇 |
综合类 | 22篇 |
预防医学 | 216篇 |
眼科学 | 208篇 |
药学 | 506篇 |
中国医学 | 4篇 |
肿瘤学 | 727篇 |
出版年
2023年 | 29篇 |
2022年 | 63篇 |
2021年 | 127篇 |
2020年 | 67篇 |
2019年 | 90篇 |
2018年 | 147篇 |
2017年 | 109篇 |
2016年 | 138篇 |
2015年 | 155篇 |
2014年 | 153篇 |
2013年 | 236篇 |
2012年 | 378篇 |
2011年 | 425篇 |
2010年 | 238篇 |
2009年 | 210篇 |
2008年 | 367篇 |
2007年 | 405篇 |
2006年 | 401篇 |
2005年 | 419篇 |
2004年 | 395篇 |
2003年 | 406篇 |
2002年 | 393篇 |
2001年 | 160篇 |
2000年 | 189篇 |
1999年 | 149篇 |
1998年 | 132篇 |
1997年 | 91篇 |
1996年 | 75篇 |
1995年 | 50篇 |
1994年 | 70篇 |
1993年 | 55篇 |
1992年 | 127篇 |
1991年 | 113篇 |
1990年 | 105篇 |
1989年 | 95篇 |
1988年 | 102篇 |
1987年 | 88篇 |
1986年 | 64篇 |
1985年 | 79篇 |
1984年 | 39篇 |
1983年 | 35篇 |
1982年 | 27篇 |
1979年 | 24篇 |
1978年 | 25篇 |
1975年 | 20篇 |
1974年 | 19篇 |
1971年 | 22篇 |
1970年 | 22篇 |
1969年 | 23篇 |
1967年 | 23篇 |
排序方式: 共有7515条查询结果,搜索用时 31 毫秒
161.
Impact of the Charlson comorbidity index and prognostic nutritional index on prognosis in patients with early gastric cancer after endoscopic submucosal dissection
下载免费PDF全文
![点击此处可从《Digestive endoscopy》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Naoto Iwai Osamu Dohi Yuji Naito Yutaka Inada Akifumi Fukui Shun Takayama Kazuyuki Ogita Kei Terasaki Takahiro Nakano Tomohiro Ueda Tetsuya Okayama Naohisa Yoshida Kazuhiro Katada Kazuhiro Kamada Kazuhiko Uchiyama Takeshi Ishikawa Osamu Handa Tomohisa Takagi Hideyuki Konishi Nobuaki Yagi Yoshito Itoh 《Digestive endoscopy》2018,30(5):616-623
162.
Yufeng Wang Yasuhiro Kuramitsu Byron Baron Takao Kitagawa Junko Akada Kazuhiro Tokuda Dan Cui Kazuyuki Nakamura 《Oncotarget》2015,6(28):25252-25265
Calcium ions (Ca2+) are indispensable for the physiology of organisms and the molecular regulation of cells. We observed that CGK733, a synthetic chemical substance, induced non-apoptotic cell death and stimulated reversible calcium sequestration by vesicles in pancreatic cancer cells. The endoplasmic reticulum (ER) stress eukaryotic translation initiation factor 2-alpha kinase 3/C/EBP homologous protein (PERK/CHOP) signaling pathway was shown to be activated by treatment with CGK733. Ionomycin, an ER stress drug and calcium ionophore, can activate PERK/CHOP signaling and accelerate CGK733-induced calcium sequestration. Knockdown of CHOP diminished CGK733-induced vesicular calcium sequestration, but had no effects on the cell death. Proteomic analysis demonstrated that the ER-located calcium-binding proteins, calumenin and protein S100-A11, were altered in CGK733-treated cells compared to non-treated controls. Our study reveals that CGK733-induced intracellular calcium sequestration is correlated with the PERK/CHOP signaling pathway and may also be involved in the dysregulations of calcium-binding proteins. 相似文献
163.
Yudai Fujiwara Hidekatsu Kuroda Tamami Abe Kazuyuki Ishida Takuma Oguri Sachiyo Noguchi Tamotsu Sugai Naohisa Kamiyama Yasuhiro Takikawa 《Ultrasound in medicine & biology》2018,44(11):2223-2232
The purpose of our study was to evaluate the diagnostic accuracy of the ultrasound-guided attenuation parameter (UGAP) for the detection of hepatic steatosis in comparison with the controlled attenuation parameter (CAP), using histopathology as the reference standard. We prospectively analyzed 163 consecutive chronic liver disease patients who underwent UGAP, CAP, computed tomography and a liver biopsy on the same day between April 2016 and July 2017. Radiofrequency signals corresponding to the images were compensated by the reference signal previously measured from the uniform phantom with known attenuation (0.44 dB/cm/MHz). The attenuation coefficient was calculated from the signals’ decay slope. The median attenuation coefficient values in patients with S0 (n?=?62), S1 (n?=?63), S2 (n?=?23) and S3 grade (n?=?15) were 0.485, 0.560, 0.660 and 0.720, respectively. Significant correlations were found between attenuation coefficient and percentage steatosis, CAP values and liver-to-spleen computed tomography attenuation ratio (p < 0.001). The areas under the receiver operating characteristic curve of UGAP for identifying ≥S1, ≥S2 and ≥S3 were 0.900, 0.953 and 0.959, respectively, which were significantly better than the results obtained with CAP for identifying ≥S2 and ≥S3. In conclusion, UGAP had high diagnostic accuracy for detecting hepatic steatosis in patients with chronic liver disease 相似文献
164.
165.
Takafumi Sai Kazuyuki Uchida Hiroyuki Nakayama 《Experimental and toxicologic pathology》2013,65(4):365-373
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) induces damage to the nigrostriatal system and subventricular zone (SVZ) of mice. While there have been many researches on the neurotoxicity of MPTP in adult mice, there have been few reports concerning that in embryonic and newborn mice. Very recently, we revealed that such neurotoxicity of MPTP and 1-methyl-4-phenylpyridinium (MPP+), a metabolite of MPTP, is observed not only in adult mice but also in embryonic and newborn mice; however, the mechanism of acute toxicity is not well elucidated. In the present study, we attempted to reveal the involvement of monoamine oxidase B (MAO-B) in the metabolism of MPTP to MPP+ and dopamine transporter (DAT) in the neuronal cellular uptake of MPP+ during the acute toxicity of MPTP in both embryonic and newborn mice. Immunohistochemistry and double-labeling immunofluorescent staining demonstrated an increase of MAO-B-positive glial cells in the brain only in MPTP-treated mice, indicating the involvement of MAO-B in the metabolism of MPTP to MPP+ during the acute neurotoxicity of MPTP in both embryonic and newborn mice. The expression of DAT was not observed in the nigrostriatal zone of embryonic mice and in the zone and SVZ of newborn mice. The mechanism of how MPP+ is taken up into those neuronal cells remains unknown. In conclusion, MAO-B is involved in the acute neurotoxicity of MPTP in embryonic and newborn mice. 相似文献
166.
Kun Tae Ahn Kazuo Yamanaka Atsushi Iwakura Keiichi Hirose Daisuke Nakatsuka Takayoshi Kusuhara Jin Ikarashi 《Annals of thoracic and cardiovascular surgery》2015,21(1):66-71
Purpose: We investigated the influence of intraoperative continuous tranexamic acid (TA) infusion on the amount of blood transfusion required in emergency surgery for type A acute aortic dissection.Methods: The study was based on the data of 55 consecutive patients who underwent surgery for type A acute aortic dissection. The patients were divided into 2 groups for comparison: Group T, consisting of 26 patients who received intraoperative continuous infusion of TA, and Group N, consisting of 29 patients who did not receive TA infusion during the surgery.Results: The mean amounts of blood transfusion required during and after surgery were compared between the 2 groups: they were 10.5 ± 8.7 and 16.2 ± 10.0 units of mannitol-adenine-phosphate-added red cell concentrate, 9.3 ± 8.6 and 17.1 ± 10.0 units of fresh frozen plasma, and 20.4 ± 12.2 and 29.7 ± 14.9 units of platelet concentrate, respectively, in Groups T and N. Thus, the amount of each of these blood products required was significantly reduced in Group T.Conclusions: During emergency surgery for type A acute aortic dissection, continuous infusion of TA resulted in a significant reduction in the amount of blood transfusion required. 相似文献
167.
Mitsuo Kawano Keiichi Yamanaka Akisa Yamagiwa Esteban C. Gabazza Katsuhiko Ando Hitoshi Mizutani 《The Journal of dermatology》2015,42(11):1066-1071
Rickettsia diseases, including Japanese spotted fever (JSF), are serious infections. Delayed diagnosis occasionally results in life‐threatening liver disorders and disseminated intravascular coagulation (DIC). Because of the shortness of the latent period, serological diagnosis is not preferable for early diagnosis of JSF. Until now, a polymerase chain reaction (PCR)‐based diagnosis method has been used for early diagnosis, and the sensitivity reaches as high as 90% using skin biopsy samples as we previously reported. On the other hand, the sensitivity of the same PCR method using blood samples is limited at less than 50%. In the present study, using peripheral blood samples, we developed a novel diagnostic method for JSF using a Rick PCR system with original PCR primers, showing improved sensitivity compared with the conventional nested PCR. It may constitute a preferable diagnostic tool for early and sensitive diagnosis of Rickettsia infection. 相似文献
168.
Tomoyuki Nakamura Ryosuke Yamaguchi Akifusa Wada Kazuyuki Takamura Haruhisa Yanagida Toru Yamaguchi 《Journal of orthopaedic science》2021,26(4):644-649
BackgroundAlthough acetabular dysplasia is a common etiology of osteoarthritis of the hip regardless of the history of developmental dysplasia of the hip (DDH), whether or not corrective surgeries are beneficial for the childhood asymptomatic acetabular dysplasia remains controversial due to a lack of evidence. We conducted a longitudinal study to compare the cartilaginous morphology on childhood magnetic resonance imaging (MRI) and the mature hip morphology of the same patient and to assess the predictive indicators for future acetabular dysplasia.MethodsA total of 92 unaffected hips (47 unilateral DDH and 45 unilateral Legg-Calvé-Perthes disease) were reviewed for X-ray and MRI findings on childhood (mean age: 6.0 years) and X-ray findings from a skeletally mature age with a mean follow-up period of 15.1 years. The following parameters were measured and compared: the immature-acetabular index (AI) and center edge angle (CE) on immature X-ray; the cartilage- and bone- AI, CE, Sharp and acetabular head index (AHI) on childhood MRI; and the mature-acetabular roof obliquity (ARO), CE, Sharp and AHI on skeletally mature X-ray. The prognostic factors on childhood MRI for acetabular dysplasia, defined by a CE of <20° on skeletally mature X-ray were also assessed.ResultsPositive correlations were shown between the cartilage-AI and mature-ARO (7.6°/6.3°; r = 0.44), the cartilage-CE and mature-CE (27.8°/28.0°; r = 0.62), the cartilage-Sharp and mature-Sharp (44.4°/41.8°; r = 0.52) and the cartilage-AHI and mature-AHI (78.7%/80.3%; r = 0.46). A multivariate analysis indicated cartilage-CE to be an independent predictor for acetabular dysplasia with a cut-off value of 22°. Children with a cartilage-CE <22° developed more frequently acetabular dysplasia compared to the others (52.4% vs. 1.4%).ConclusionsChildhood MRI findings are useful for the prediction of acetabular dysplasia without a DDH history. Children with a cartilage-CE ≥23° are likely to achieve a non-dysplastic hip without the need for surgical intervention. 相似文献
169.
Tsuyoshi Ito Kazuhiko Suzuki Kazuyuki Uchida Hiroyuki Nakayama 《Experimental and toxicologic pathology》2012,64(7-8):761-765
Intraperitoneal 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) administration induces apoptosis of subventricular zone (SVZ) doublecortin (Dcx)-positive neural progenitor cells (migrating neuroblasts, A cells). Actually, a metabolite of MPTP, 1-methy-4-phenylpiridinium (MPP+), is responsible for neural progenitor cell toxicity. In the present study, to examine whether the MPTP-induced SVZ cell apoptosis is caused directly by MPP+ metabolized through monoamine oxidase B (MAO-B), MPTP or MPP+ was intracerebroventricularly (icv) injected into C57BL/6 mice. At Day 1 postinjection, many terminal deoxynucleotidyl transferase-mediated dUTP endlabeling (TUNEL)-positive cells were observed in the SVZ of both low (36 μg) and high (162 μg) dose MPTP- and MPP+-injected mice. The number of Dcx-positive A cells showed a significant decrease following high dose of MPTP- or MPP+-injection on Days 1 and 3, respectively, whereas that of EGFR-positive C cells showed no change in mice with any treatment. In addition, prior icv injection of a MAO-B inhibitor, R(?)-deprenyl (deprenyl), inhibited MPTP-induced apoptosis, but not MPP+-induced apoptosis. MAO-B- and GFAP-double positive cells were detected in the ependyma and SVZ in all mice. It is revealed from these results that icv injection of MPTP induces apoptosis of neural progenitor cells (A cells) in the SVZ via MPP+ toxicity. In addition, it is suggested that the conversion from MPTP to MPP+ is caused mainly by MAO-B located in ependymal cells and GFAP-positive cells in the SVZ. 相似文献
170.