CD44v6 expression in patients with stage II or stage III sporadic colorectal cancer is superior to CD44 expression for predicting progression

Background: Currently, it is difficult to predict the prognosis of patients exhibiting stage II or stage III colorectal cancer (CRC) and to identify those patients most likely to benefit from aggressive treatment. The current study was performed to examine the clinicopathological significance of CD44 and CD44v6 protein expression in these patients. Study design: We retrospectively investigated 187 consecutive patients who underwent surgery with curative intent for stage II to III CRC from 2007 to 2013 in the Beijing Civil Aviation Hospital. CD44 and CD44v6 protein expression levels were determined using immunohistochemistry and compared to the clinicopathological data. Results: Using immunohistochemical detection, CD44 expression was observed in 108 (57.75%) of the CRC patients; and its detection was significantly associated with greater invasion depth, lymph node metastasis, angiolymphatic invasion, and a more advanced pathological tumor-lymph node-metastasis (TNM) stage. CD44v6 expression was observed in 135 (72.19%) of the CRC patients; and its expression was significantly associated with a poorly differentiated histology, greater invasion depth, lymph node metastasis, angiolymphatic invasion, and a more advanced pathological TNM stage. Expression of CD44v6 was higher than that of CD44 in stage II and stage III sporadic CRC. Conclusion: CD44v6 is a more useful marker for predicting a poor prognosis in stage II and stage III sporadic CRC as compared to CD44.     

新生儿细菌L型感染途径初探

0　引言　细菌 L型是细菌的缺壁状态 ,不合理的抗生素应用是导致细菌 L 型形成和成年人细菌 L 型感染的主要原因 [1 ] .新生儿感染细菌 L 型的途径尚不清楚 .我们通过对新生儿外周血及其母体血和乳汁进行细菌和细菌 L 型培养 ,探讨细菌 L型对新生儿的感染途径 .1　材料和方法1 .1　材料　标本取自我院 1 997- 0 8～ 1 998- 0 8间住院的 5 8例不明原因发热新生儿的外周血及其母体外周血和乳汁 .常规配制普通增菌培养基 ,普通琼脂血平板 . 1 L型液体培养基 :牛肉浸液 80 0 m L,蛋白胨 (日本产 ) 2 g,Na Cl4g,蔗糖 2 0g,混匀 ,加热溶解 ,调…     

Induction of high-titer IgG antibodies against multiple leukemia-associated antigens in CML patients with clinical responses to K562/GVAX immunotherapy

The ability to target myeloid leukemia with immunotherapy would represent a significant therapeutic advance. We report here immunological analysis of clinical trials of primary and secondary vaccination with K562/GM-CSF immunotherapy in adult chronic phase chronic myeloid leukemia patients (CML-CP) with suboptimal responses to imatinib mesylate. Using serological analysis of recombinant cDNA expression libraries of K562 with autologous vaccinated patient serum, we have identified 12 novel chronic myeloid leukemia-associated antigens (LAAs). We show that clinical responses following K562/GM-CSF vaccination are associated with induction of high-titer antibody responses to multiple LAAs. We observe markedly discordant patterns of baseline and induced antibody responses in these identically vaccinated patients. No single antigen was recognized in all responses to vaccination. We demonstrate that an additional ‘booster'' vaccination series can be given safely to those with inadequate responses to initial vaccination, and is associated with more frequent induction of IgG responses to antigens overexpressed in K562 vaccine compared with primary CML-CP. Finally, those with induced immune responses to the same LAAs often shared HLA subtypes and patients with clinical responses following vaccination recognized a partially shared but non-identical spectrum of antigens; both findings have potentially significant implications for cancer vaccine immunotherapy.     

体外循环心脏不停跳与非体外循环冠状动脉搭桥术治疗冠状动脉三支病变的疗效

目的 探讨心肺转流(CPB)下行心脏不停跳冠状动脉搭桥术(OPBH)与非体外循环下冠状动脉搭桥术(OPCAB)治疗冠状动脉三支病变的临床效果.方法 210例单纯冠状动脉三支病变患者分为0PCAB组(105例)和OPBH组(105例),分别进行临床观察.结果 OPCAB组无一例需转成OPBH.所有患者均在心脏侧壁和后壁区域搭桥.两组人均冠状动脉远端吻合数和再血管化指数相似;手术后呼吸支持时间、胸腔引流量和输血量OPCAB组明显少于OPBH组;围手术期死亡率和并发症发生率两组比较差异无统计学意义.结论 对于低左室射血分数的患者,虽然出血和输血量较大,术后呼吸支持时间、ICU停留时间较长,但OPBH能取得与OPCAB相同的疗效.     

HPLC法同时测定牙周康胶囊中甲硝唑和芬布芬的含量

目的 :建立测定牙周康胶囊中甲硝唑和芬布芬含量的方法。方法 :高效液相色谱法。固定相为十八烷基硅烷键合硅胶 ,流动相 :甲醇 0 .0 1mol/L十二烷基硫酸钠 ( 63∶37) (用磷酸调节pH值至 2 .5) ,检测波长为 2 95nm .结果 :同时测定甲硝唑和芬布芬的含量。甲硝唑平均回收率为 99.8% ,n =6,RSD为 0 .4 0 %。芬布芬平均回收率为1 0 0 .1 % ,n =6,RSD为 0 .36%。结论 :本方法简便、快速、可靠 ,可用于牙周康胶囊中甲硝唑和芬布芬的含量测定     

辅料对X线造影剂碘海醇注射液稳定性的影响

目的：考察不同辅料的国产碘海醇（Ｉｏｈｅｘｏｌ）注射液稳定性的影响。方法：测定高温灭菌前后６组不同辅料的碘海醇注射液ＰＨ、碘离子浓度、含量变化。结果与结论：同时含三羟甲基氨基甲烷（Ｔｒｉｓ,１．２ｍｇ／ｍｌ）、乙二胺四乙酸二钠钙（ＥＤＴＡ－Ｎａ２Ｃａ,０．１ｍｇ／ｍｌ）２种辅料时,碘海醇注射液最稳定。     

RP-HPLC法同时测定益肺佳营养液中维生素C和维生素B6的含量

目的 :建立了同时测定益肺佳营养液中维生素C和维生素B6含量的方法。方法 :高效液相色谱法。固定相为十八烷基硅烷键合硅胶 ;流动相为 0 .0 1mol/L磷酸二氢钾溶液 (用磷酸调节PH至 2 .5)。检测波长 :①维生素C紫外检测 ,波长 2 4 2nm ;②维生素B6荧光检测 ,激发波长 2 90nm ,发射波长 395nm。结果 :线性范围 :维生素C 2 0 .5～1 0 2 .3μg/ml,r =0 .9999;维生素B60 .2 8～ 1 .4 2 μg/ml,r=0 .9999。方法回收率 :维生素C为 99.1 %,n =5,RSD为0 .4 8%;维生素B6为 1 0 0 .2 %,n =5,RSD为 1 .2 1 %。结论 :该方法操作简便快速 ,灵敏度高 ,结果准确 ,适用于该制剂中维生素C和维生素B6的含量测定。     

Anemoside A3 Enhances Cognition through the Regulation of Synaptic Function and Neuroprotection

Compounds that have the ability to both strengthen synaptic function and facilitate neuroprotection are valuable cognitive enhancers that may improve health and quality of life, as well as retard age-related cognitive deterioration. Medicinal plants are an abundant source of potential cognitive enhancers. Here we report that anemoside A3 (AA3) isolated from Pulsatilla chinensis modulates synaptic connectivity in circuits central to memory enhancement. AA3 specifically modulates the function of AMPA-type glutamate receptors (AMPARs) by increasing serine phosphorylation within the GluA1 subunit, which is a modification required for the trafficking of GluA1-containing AMPARs to synapses. Furthermore, AA3 administration activates several synaptic signaling molecules and increases protein expressions of the neurotrophin brain-derived neurotrophic factor and monoamine neurotransmitters in the mouse hippocampus. In addition to acting through AMPARs, AA3 also acts as a non-competitive NMDA receptor (NMDAR) modulator with a neuroprotective capacity against ischemic brain injury and overexcitation in rats. These findings collectively suggest that AA3 possesses a unique ability to modulate the functions of both AMPARs and NMDARs. Concordantly, behavioral studies indicate that AA3 not only facilitates hippocampal long-term potentiation but also enhances spatial reference memory formation in mice. These multifaceted roles suggest that AA3 is an attractive candidate for further development as a cognitive enhancer capable of alleviating memory dysfunctions associated with aging and neurodegenerative diseases.     

土贝母皂苷原料及其注射液中土贝母总皂苷的含量测定

目的：建立土贝母总皂甙含量测定。方法：用５％香草醛冰醋酸溶液－脱水硫酸为显色剂,在９０℃加热显色,用分光光度法测定土贝母皂苷原料及其注射液中土贝母总皂苷的含量。结果：平均回收率为１０１．３９％,ＲＳＤ＝１．９１％（ｎ＝５）。结论：该法可作为土贝母皂甙原料及其注射液的定量控制方法之一。     

双氢青蒿素对小鼠抗疟作用的药效动力学

双氢青蒿素(DHA)是青蒿素的一种还原产物,对感染伯氏疟原虫ANKA株的小鼠一次im给药,其抗疟作用的量—效关系和时—效关系可分别用y=4.9960+2.9536x和y=7.2654-0.3414t表达,进而估算出其ED₅₀和ED₅₀分别为1.00±0.13 mg/kg和2.72±0.70 mg/kg以DHA 5.0 mg/kg im后其药效下降一半的时间为6.6 h,体内有效药量的消除速率常数k为0.2662 h^-1,效量半衰期为2.6 h。式中y为机率单位,是DHA对疟原虫抑制作用的估算值,x为对数剂量,t为DHA im后的间隔时间。