Effect of superantigen on ion electrophysiology and permeability in rabbit maxillary sinus epithelia

Objective Superantigens are potent inflammatory stimuli which derive from pathogenic microbes such as bacteria, viruses and protozoa. The aim of this study was to investigate the role of superantigens on the function of rabbit maxillary sinus epithelium. Methods Twenty New Zealand white rabbits were divided into 4 groups. Rabbit sinus mucosa was separated under a surgical microscope and mounted in Ussing chambers to record short circuit current, conductance and permeability to horseradish peroxidase (HRP). Group A was used as normal control. Group B was stimulated with an injection of superantigen into the sinus for 4 hours. The sinus mucosa of Group C was stimulated by the addition of tumor necrosis factor α (TNF-α) into Ussing chambers. Group D sinus mucosa was stimulated by superantigen after pretreatment with anti-TNF-α antibody. Results Superantigen evoked increases in sinus epithelial cell baseline short circuit current, conductance and permeability to HRP stimulated by the addition of TNF-α into Ussing chambers. These were similar to results from superantigen stimulation in vivo. The effect of superantigen on sinus epithelial cells could be blocked by pretreatment with anti-TNF-α antibody. Conclusions Superantigen affected the function of sinus epithelial cells, including the capability of epithelial defensive barrier, which might be mediated by TNF-α.     

烟雾吸入伤后肺泡上皮液体清除机能的变化及其意义

观察烟雾吸入伤后肺泡上皮液体清除功能的变化及其意义。方法 :Wistar大鼠 4 2只 ,随机分为正常对照组 (C组 )、烟雾致伤组 (I组 )、阿咪洛利组 (A组 )、哇巴因组 (O组 )、阿咪洛利加哇巴因组 (AO组 )和特普他林组 (T组 )。采用大鼠烟雾吸入伤模型。伤后 2 4h测定肺泡内液体清除率 (ALC)、总肺水量 (TLW )和肺血管外肺水量 (EVLW )。结果 :I组ALC降低 4 6.8% ,TLW和EVLW进一步增加 ;AO组ALC则降至最低点 ,TLW和EVLW升至最高点。而T组ALC明显增高 ,TLW和EVLW显著减少。ALC与TLW和EVLW之间显著相关。结论 :肺泡上皮液体清除功能障碍是烟雾吸入伤后早期肺水肿形成的因素之一。     

人载脂蛋白E基因型测定方法的建立

目的：建立一种准确、简便和经济的适用于大范围测定载脂蛋白Ｅ基因型的方法。方法：自中血中采用ＴＫＭ法提取人基因组ＤＮＡ,ＰＣＲ－ＲＦＬＰ确定载脂蛋白Ｅ的基因型,硝酸银染色聚丙烯酰胺凝胶,若银染效果不佳可用硝酸脱色后重染。结果：ＴＫＭ法获得ＤＮＡ在产量和质量上均能满足ＰＣＲ需求;电泳凝胶经银染可得清晰的ＤＮＡ特异带,据此能准确确定样品载脂蛋白Ｅ的基因基因型;用硝酸溶液脱色后的凝胶经重染仍可得到清晰的图     

Screening and identification of 3-aryl-quinolin-2-one derivatives as antiviral agents against influenza A

Quinolin-2-one represents an important and valuable chemical motif that possesses a wide variety of biological activities; however, the anti-influenza activities of quinolin-2-one-containing compounds were rarely reported. Herein, we describe the screening and identification of 3-aryl-quinolin-2-one derivatives as a novel class of antiviral agents. The 3-aryl-quinolinone derivatives were synthesized via an efficient copper-catalyzed reaction cascade that we previously developed. Using this synthetic method, preliminary structure–activity relationships of this scaffold against the influenza A virus infection were systematically explored. The most potent compound 34 displayed IC₅₀ values of 2.14 and 4.88 μM against the replication of H3N2 (A/HK/8/68) and H1N1 (A/WSN/33) strains, respectively, without apparent cytotoxicity on MDCK cells. We further demonstrated that 27 and 34 potently inhibited the plaque formation of the IAV, rendering this scaffold attractive for pursuing novel anti-influenza agents.     

抑那通在前列腺癌内分泌治疗中的临床意义

目的为提高晚期前列腺癌内分泌治疗效果,１９９０年７月～１９９２年７月间对１７例前列腺癌患者进行了抑那通（药物除睾）的临床观察。方法随访３～５年获得可评价资料１６例病例,采用免疫酶联法测定ＰＳＡ变化,放免法检测性激素水平,用Ｉ－ＰＳＳ表调查排尿及生活质量,对前列腺及转移病灶、全身状况的变化进行对比观察。结果抑那通治疗后睾酮降至去势水平（Ｐ＜０．０１）,前列腺特异抗原及其他肿瘤标记物提示反应性良好,症状及全身状况明显改善。结论每月注射一次抑那通,如果与抗男性素剂联合使用可获得更安全有效的治疗效果。     

Epidemiological characteristics of uveitis in Switzerland

Since January 1990, data from uveitis patients have been systematically stored in a computer data bank. During the period from January 1990 to March 1993, 435 new patients (185 female and 250 male, mean age 43 years; range 6–92) were seen at the Uveitis Clinic of the Hopital Jules Gonin. These 435 patients (630 eyes) were subdivided into anterior uveitis (268 patients — 62%), intermediate uveitis (47 patients — 11%), posterior uveitis (89 patients — 20%) and panuveitis (31 patients — 7%). The incidence of uveitis for the referral area considered was calculated to be 17 per 100,000 inhabitants per year. A specifie diagnosis was found in 312 cases (72%). The most frequently diagnosed entities were HLA-B27-associated acute anterior uveitis (67 cases — 15.4%), uveitis associated with acute herpes zoster ophthalmicus (40 cases — 9.2%), toxoplasmosis (39 cases — 9%), typical pars planitis (29 cases — 6.7%), sarcoidosis (29 cases — 6.7%), Fuchs' heterochromic cyclitis (27 cases — 6.2%), herpetic anterior uveitis (21 cases — 4.8%) and acute retinal necrosis (11 cases — 2.5%). Incidence and distribution of most disease entities correspond to those of other European series.     

电教多媒体数据库管理系统的设计

目的：设计研制一个面向院校电教中心的多媒体数据库管理系统,以便用现代信息技术管理电教中心众多的信息资料。方法：在国产MIS开发平台Multibase2．0上,用原型法进行无编程设计开发。结果：制作的数据库管理系统运行稳定,操作方便,维护简单,查询功能新颖而强大,基本功能设计具有较强的针对性,能够满足电教中心日常工作的需要。结论：采用国产无编程MIS开发平台Multibase完全可以开发出实用的多媒体数据库管理系统,本数据库管理系统可向军内外院校推广使用。     

侧柏叶乙酸乙酯提取物对豚鼠离体气管平滑肌的作用

用离体豚鼠气管条,探讨侧柏叶乙酸乙酯提取物对气管平滑肌作用。结果表明,侧柏叶乙酸乙酯提取物能抑制乙酰胆碱,氯化钾所致气管平滑肌收缩,而且能使乙酰胆碱收缩气管平没肌的量效曲线右移,并抑制最大效应,其作用为剂量依赖性心肌肥厚提示侧柏叶乙酸乙酯提取物松驰气管平滑肌作用机制可能与影响Ｃａ＾２＋的跨膜转运有关。     

NADPH-diaphorase-containing neurons in cortex, subcortical white matter and neostriatum are selectively spared in Alzheimer's disease

To investigate the involvement of NADPH-diaphorase (NADPH-d)-containing neurons in Alzheimer's disease (AD), NADPH-d enzyme histochemistry in vibratome sections was applied to the superior frontal and superior temporal cortex and the neostriatum in 5 AD and 6 aged control brains. Overall there was a neuronal loss and atrophy in the cortex of AD. Despite slight morphological neuronal changes in the cortex of AD, we found no significant difference in the number of NADPH-d-positive neurons in both cortex and neostriatum between control and AD cases. These results provide further evidence for a selective preservation of NADPH-d neurons in AD. In order to check whether nNOS-immunoreactive neurons are identical to NADPH-d-positive neurons in the human brain, we examined the frontal and temporal cortex and neostriatum of normal human brains in serial cryostat sections. We found that nNOS-containing neurons paralleled NADPH-d-positive neurons in these brain regions. Copyrightz1999S.KargerAG,Basel