大鼠血清转铁蛋白受体及促红细胞生成素水平在高住低训过程中的变化

目的:"高住低训"过程中红细胞变化过程和机制还不完全清楚。实验模拟"高住低训"训练法,观察高住低训习服过程中大鼠血清转铁蛋白受体及促红细胞生成素水平的动态变化。方法:实验于2007-06在辽宁师范大学体育学院运动生理实验室进行。①实验材料:50只健康SD大鼠由大连医科大学实验动物中心提供,雌雄不分,适应性喂养1周后随机分为2组:常氧对照组(n=10)、高住低训组(n=40)。②实验方法:高住低训组大鼠每天在模拟2500m的高原环境中生活,并模拟海拔1250m环境游泳运动1h,6 d/周,共4周。③实验评估:训练第1,2,3,4周检测大鼠红细胞数及血红蛋白浓度,采用免疫散射比浊法测定血清转铁蛋白受体。结果:50只大鼠均完成实验,并进入结果分析。与常氧对照组相比,高住低训第1周的促红细胞生成素水平明显提高(P<0.01),高住低训第2周和第3周促红细胞生成素水平仍处在较高水平(P<0.05),血清转铁蛋白受体、血红蛋白和红细胞水平明显升高,并达到峰值(P<0.01),高住低训第4周血清转铁蛋白受体的水平仍处在较高水平(P<0.05),血红蛋白和红细胞水平仍继续升高(P<0.01)。结论:高住低练能使大鼠血清转铁蛋白受体、促红细胞生成素和血红蛋白及红细胞水平均明显增加。     

半侧空间忽略症状与基底核区脑卒中患者肢体功能恢复的关系

目的:观察有和无半侧中间忽略症状基底核脑卒中的患者日常生活活动能力差异。方法:①选择2002-11/2003-11吉林大学第一医院康复科住院基底核区脑卒中患者25例。均符合第四届全国脑血管病学术会议修订的急性脑梗死诊断标准,并经CT或MRI证实;均对观察目的知情同意。设有半侧空间忽略症状患者12例为阳性组,其中男4例,女8例;平均年龄(62±8)岁;全部为右基底核区脑卒中。无半侧空间忽略症状患者13例为阴性组,其中男10例,女3例;平均年龄(66±8)岁;左侧基底核区脑卒中为5例,右侧基底核区脑卒中为8例。②于患者入院、出院时采用Barthel指数分级法对日常生活活动能力进行评分,每周进行1次。该分级法有10项内容,分3级:<40分为差,依赖较明显或完全依赖;41～60分为中,有功能障碍,稍依赖;总分>60分为良。整个治疗过程中给予患者全面的康复功能训练,包括物理疗法、作业疗法和语言训练。同时对症治疗。结果:基底核区脑卒中患者25例均进入结果分析。入、出院时阳性组Barthel指数均明显低于阴性组[阳性组:(22.50±13.46),(62.83±14.72)分;阴性组:(37.62±11.98),(79.69±10.59)分,P<0.01]。出院时Barthel指数提高度差异不明显[阳性组:(40.33±19.20)分;阴性组:(42.08±11.11)分,P>0.05]。阳性组住院时间明显长于阴性组[(79.83±67.99),(34.15±18.80)d,P<0.01]。结论:①基底核脑卒中出现半侧空间忽略症状的患者日常生活活动能力差。②伴有和不伴有半侧空间忽略症状基底核脑卒中患者经过治疗日常生活活动能力改善程度相近。     

The effects of three recombinant growth factors, IL-3, GM-CSF, and G- CSF, on the blast cells of acute myeloblastic leukemia maintained in short-term suspension culture

The blast stem cells of acute myeloblastic leukemia (AML) respond in cell culture to growth factors by both self-renewal and terminal divisions. Both of these functions have been shown to be stimulated by the recombinant growth factors granulocyte-macrophage colony- stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF). In this paper, recombinant gibbon interleukin-3 (IL-3), homologous to human IL-3, was tested on blast cells and compared with the effects of GM-CSF, G-CSF, and medium conditioned by the bladder cell line 5637 (5637-CM). We found that IL-3 was an effective stimulator of blast renewal and terminal divisions. However, great patient-to-patient variation was found. A graphic method of presenting complex comparisons between growth factors is also included.     

单克隆抗体与平阳霉素偶联物对肿瘤的区域性导向实验治疗

以小鼠H22肝癌腹腔内移植和胸腔内移植肿瘤为模型,观察3A5-PYM偶联物对腔内肿瘤的治疗作用,并以裸鼠移植结肠癌为模型,观察局部注射的治疗作用。结果表明,于小鼠肝癌H22皮下肿瘤局部给药,3A5-PYM在肿瘤的浓度较游离PYM高,滞留时间也较长。对小鼠腹腔内或胸腔内移植的肿瘤,腔内注射3A5-PYM比游离PYM具有更显著的延长动物生存期的作用。裸鼠皮下移植HT-29人结肠癌,在肿瘤周围注射3A5-PYM比iv或ip给予3A5-PYM显示更高的抑瘤率。提示单抗与平阳霉素偶联物在肿瘤的区域性导向治疗中有较好的疗效。     

Schistosoma mansoni excretory circulating cathodic antigen shares Lewis- x epitopes with a human granulocyte surface antigen and evokes host antibodies mediating complement-dependent lysis of granulocytes

Parasitic worms of the genus Schistosoma excrete relatively large amounts of immunogenic glycoproteins (circulating cathodic antigen [CCA]) that contain polysaccharide side chains with the trisaccharide Lewis-x (L(ex)) as a repeating unit. These carbohydrates evoke high titers of specific IgM antibodies that cross-react with the repeating L(ex) units on the surface of granulocytes. Consequently this might lead, in the presence of complement, to lysis of the granulocytes. In the present study, this hypothesis was investigated using anti-CCA mouse monoclonal antibodies (MoAbs) and polyclonal antibodies purified from sera of infected humans. By flow cytometry, it was demonstrated that the mouse MoAbs directed against CCA strongly recognized the granulocytes. It could also be shown that these MoAbs, as well as anti- CCA IgM antibodies purified from infected human sera, caused lysis of granulocytes in a complement-dependent cytotoxicity assay. Sera from healthy controls or from patients with other helminth infections resulted in negligible granulocytotoxicity. These in vitro observed phenomena may explain the mild to moderate neutropenia that occurs in schistosomiasis patients.     

大鼠单克隆抗体与博来霉素A6偶联物治疗人大肠癌实验研究

用Dextran T-40为中介体偶联大鼠抗人大肠癌单克隆抗体R19和博来霉素A6。体外实验显示R19与A6偶联物对人盲肠癌细胞Hce-8693抑制50%克隆生成浓度(IC₅₀)为0.019μmol/L;游离A6及无关单抗与A6偶联物M3-A6分别为1.05和1.00μmol/L;对人结肠HT-29细胞IC₅₀为0.078μmol/L,而游离A6为4.0μmol/L。同时加入R19单抗能阻断R19-A6偶联物的细胞毒性。体内实验显示:R19-A6偶联物对裸鼠移植的人盲肠癌生长的抑制率达90%,而同等剂量的游离A6,R19与A6混合物和M3-A6的抑制率分别为52,34和48%。结果表明单抗R19与A6偶联物对人大肠癌的抑制作用明显比游离A6强。     

Rp-HPLC法测定血清中的抗生育甾体药物利洛司酮

以RU₄₈₆为内标,建立了血清样品中新型抗孕激素甾体药物利洛司酮(lilopristone)的反相高效液相色谱法。色谱分析条件为μBondapakC₁₈柱10μm,300mm×3.9mmID;流动相为甲醇-二氯甲烷-10mmol·L^-1磷酸盐缓冲液(pH4.0)(67:5:28v/v);紫外检测波长为302nm。利洛司酮的检测限为1ng(S/N≥4:1)。血清样品经二次液一液超声振荡萃取后,得到了较好的净化。血清样品中利洛司酮的方法回收率为103.3%,日内精密度及日间精密度平均RSD为3.51%及2.92%。在浓度为10～1000ng·ml^-1血清范围内呈线性关系。     

Invasive cervical carcinoma: comparison of MR imaging and surgical findings

The accuracy of magnetic resonance (MR) imaging in staging invasive carcinoma of the cervix was determined retrospectively in 57 consecutive patients in whom the extent of disease was surgically confirmed. MR images were analyzed for (a) location and size of the primary tumor; (b) tumor extension to the uterine corpus, vagina, parametria, pelvic sidewall, bladder, or rectum; and (c) pelvic lymphadenopathy. The accuracy of MR imaging in determination of tumor location was 91% and for determination of tumor size within 0.5 cm, 70%. Its accuracy was 93% for vaginal extension and 88% for parametrial extension. Pelvic sidewall, bladder, and rectal involvement were accurately excluded in all patients, but the positive predictive values were 75%, 67%, and 100%, respectively. Overall, the accuracy of MR imaging in staging was 81%. MR imaging is valuable because it can accurately demonstrate tumor location, tumor size, degree of stromal penetration, and lower uterine segment involvement. It is also valuable for ruling out parametrial, pelvic sidewall, bladder, and rectal involvement.