W／H^2和W／H^3指数在儿童少年肥胖评价中的适用性

身高体重指数因其测量计算简便、误差小而在儿童少年肥胖评价中逐渐受到重视，但Ｗ／Ｈ２和Ｗ／Ｈ３在儿童少年肥胖评价中的适用性仍有争议，为了探讨两指数在儿童少年肥胖评价中的适用性，在西安市７～１８岁儿童少年中进行了本研究，结果发现在７～１３岁间Ｗ／Ｈ３指数比Ｗ／Ｈ２指数好，而在１４岁以后Ｗ／Ｈ２比Ｗ／Ｈ３更合适。     

pcDNA3.1/hIL-18真核表达载体的构建及表达

目的 :构建重组真核表达质粒pcDNA3.1/IL 18,并在哺乳动物细胞COS 7和Rlc310中进行瞬时和稳定性表达。方法 :从含hIL 18基因的中介载体 pGEM TEasy( pGEM T/hIL 18)中 ,以限制性内切酶酶切方法获得目的片段 ,克隆入真核表达质粒 pcDNA3.1( )中。以脂质体法转染COS 7和Rlc310细胞 ,用RT PCR检测IL 18mRNA的水平 ,免疫组化染色法检测蛋白表达。结果 :构建了hIL 18基因的重组真核表达质粒pcDNA3.1/IL 18,并可在哺乳动物细胞中瞬时、稳定表达 ,获得了可稳定表达hIL 18基因的Rlc310细胞株。结论 :pcDNA3.1/IL 18的构建及表达 ,为IL 18抗肿瘤作用的研究奠定了基础     

精液分析中精子密度室内质量控制方法研究

目的建立一种可行的精液密度测定的质量控制方法.方法采用血细胞计数板和计算机辅助精液分析(CASA)两种方法,对所选取的与精子头部直径相近的质控珠进行高、中、低三种密度测定,分别于日内及日间测试12次.结果采用血细胞计数板方法测试高、中、低三种密度结果的日内变异系数分别为12.09%、8.86%、4.72%,日间变异系数分别为16.71%、10.65%、10.22%.CASA法日内变异系数分别为6.65%、5.93%、3.80%,日间变异系数分别为7.90%、6.84%、6.14%.结论质控珠可用于以上两种测试精子密度方法的室内质量控制,从而达到保证实验室测试精子密度质量的目的.     

人类睾丸生精细胞凋亡相关基因TSARG3的克隆

目的：克隆人类睾丸生精细胞凋亡相关基因TSARG3。方法：从已获得的小鼠稳睾和正常睾丸对照中表达量有明显差异的表达序列标签片段（BE644537）入手，构建人同源表达序列标签重叠群，应用基因特异性引物和载体特异性引物，在睾丸cDNA文库的DN或进行巢式PCR扩增、测序，对测序结果进行生物信息学分析。结果：从睾丸cDNA文库中分离出人类睾丸凋亡相关基因的5’末端而获得全长cDNA,命名为TSARG3，GenBank登录号为AF419291（保密期为1年），同时应用相同方法克隆了该基因在小鼠中的同源基因，GenBank登录号为AF419292。结论：获得人类睾丸生精细胞凋亡相关基因TSARG3，该基因可能与人类睾丸生精细胞凋亡有关。     

心肌细胞膜通道电流及其动力学研究

建立了心肌细胞膜离子通道钠电流，钾电流和钙电流的动力数学模型，探讨了离子通道动力学机制，为进一步整合各离子电流数学模型用以研究动作电位全过程和为动物实验以及临床研究提供理论基础。     

Functional supertype of HLA-A2 in the presentation of Flu matrix p58-66 to induce CD8+ T-cell response in a Northern Chinese population

The functional supertype of HLA-A2 was investigated in the presentation of the A*0201-restricted Flu matrix p58-66 peptide to activate recall CD8+ T-cell response. In healthy Northern Chinese, the HLA-A2 supertype was mainly composed of the six alleles, A*0201 (26.4%), A*0206 (12.7%), A*0203 (8.2%), A*0207 (7.3%), A*0210 (1.8%) and A*0205 (0.9%), as analyzed by PCR using sequence specific primer (PCR-SSP) and sequence based typing (SBT). The IFN-gamma release Elispot assay was employed to assess effector CD8+ T cells. In A*0201-bearing individuals, the CD8+ T-cell response was potent when stimulated with autologous CD8- PBMCs. The frequency of the effector CD8+ T cells was 96.6% with the magnitude of effector CD8+ T cells of 225 SFC/5 x 104 CD8+ T cells and the RI of 25.7. In non-A*0201 individuals, the effector CD8+ T cells were minimally detectable while the peptide was presented by the autologous CD8- PBMCs. However, the induction of the response of CD8+ T cells obtained from non-A*0201 individuals was remarkably improved when the peptide was presented by autologous dendritic cells instead of CD8- PBMCs. The HLA-A2 alleles possessing cross-reactivity in the peptide presentation were mainly of A*0206 and non-A*0201 heterozygotes of A*0206 and A*0210. Moreover, A*0206 as the HLA-A2 functional supertype was further confirmed by tetramer assay. In two A*0206+ donors with CD8+ T-cell response to the peptide, the CD8+ T-cell frequency assessed by specific binding of peptide HLA-A*0201 tetramer was 4.62% and 1.66%, respectively. Thus, our results have substantiated the immunological relevance of the HLA-A2 supertype, which may benefit the design of peptide vaccines with the potential to be applicable in broader populations.     

一起由磷化铝引起的家庭聚集性中毒事件的调查分析

目的 分析一起由磷化铝引起的家庭聚集性中毒事件的流行病学特征,为今后处置类似事件提供科学的依据。方法 对2021年济南市商河县一起急性中毒事件开展现场流行病学调查和实验室检测。调查主要针对病例及其家属进行面访,并入户考察和采集食品、环境标本和病例标本。结果 本事件共涉及7人,其中病例6例,罹患率85.7%,病例男女比例1∶1.33（3∶4）,1例死亡。指示病例发病时间为23日20时左右,末例发病时间为24日6时左右,推测潜伏期为8～12小时。临床表现以呕吐（100%）、阵发性腹痛（100%）、恶心（43%）和腹泻（43%）为主。未发现家庭成员有可疑共同暴露餐次和可疑暴露食品。实验室在所采集的环境空气样本和死亡病例的血中检出磷化氢（PH3）。结论 本次事件是一起磷化铝使用不当引起的家庭聚集性磷化铝中毒事件,储粮间的磷化铝吸潮挥发产生的磷化氢是主要致病因素。     

The Protective Roles of Exercise and Maintenance of Daily Living Routines for Chinese Adolescents During the COVID-19 Quarantine Period

PurposeAdolescents are particularly vulnerable during the COVID-19 quarantine periods and may be at risk for developing psychological distress symptoms that extend beyond a crisis, including depression. This study examined adolescents’ postquarantine depressive symptoms associated with pandemic stressors. The primary aim was to identify potential protective factors that may buffer the association between the presence of COVID-19 cases in adolescents’ communities and their postquarantine depressive symptoms.MethodsAdolescents from public schools were recruited from Zhengzhou city, Henan, China (N = 1,487, M_age=13.14 years, 50% girls). Adolescents reported the presence of confirmed or suspected COVID-19 cases in their communities, their daily activities and routines during the 2-month quarantine period, and depressive symptoms after the quarantine period.ResultsThe presence of cases in adolescents’ communities during the quarantine contributed to more depressive symptoms in adolescents after the quarantine. This association was buffered by adolescents’ spending more time on physical activities and better maintenance of daily living routines during the quarantine period. The presence of community infection was also more strongly associated with depressive symptoms in older adolescents.ConclusionsThe presence of COVID-19 cases in communities contributed to adolescents’ poorer mental health, and the association was stronger for older adolescents. Spending time on physical activities and maintaining daily living routines during the quarantine appear to be practical strategies that can be used by adolescents to mitigate the association between pandemic stressors and their diminishing mental health.     

内蒙古地区蒙古族人HLA—DQA1基因多态性研究

目的：了解内蒙古地区蒙古族HLA-DQA1基因多态性。方法：采用PCR-SSP技术对内蒙古地区97例内蒙古族人群进行HLA-DQA1等位基因型别分析。结果：蒙古族中高频率的HLA-DQA1等位基因为*0301、*0501、*0102,最少见的为*0601。结论：蒙古族人群HLA-DQA1基因分布,既有汉族人的特点,又有其独特性。     

中草药的变态反应

通过大量的文献调查、以鱼腥草和丹参及其制剂引起的变态反应为重点,介绍常用中草药诱发变态反应的临床表现、分型、处理和预防方法。为临床安全、有效使用中草药提供借鉴。