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31.
Margaret Comerford Freda RN EdD FAAN Mary Lou Moore RN PhD FAAN 《Journal of nursing scholarship》1995,27(1):61-64
Interdisciplinary collaboration between nursing and medicine is a valued goal, but one which is difficult to achieve. Two nurses who are faculty members in medical schools reflect on their unique roles and how these encompass interdisciplinary research, teaching, and practice. 相似文献
32.
Distinct autophosphorylation sites sequentially produce autonomy and inhibition of the multifunctional Ca2+/calmodulin-dependent protein kinase 总被引:5,自引:0,他引:5
The multifunctional Ca2+/calmodulin-dependent protein kinase (multifunctional CaM kinase) may be an important mediator for neurotransmitters and hormones that utilize Ca2+ as a "second messenger." We examined the ability of autophosphorylation to convert the multifunctional CaM kinase to a Ca2+/calmodulin-independent (autonomous) form to determine whether autophosphorylation is a mechanism for short- or long-term enhancement of Ca2+ action. As the kinase incorporates phosphate during continuous stimulation by Ca2+/calmodulin, its ability to phosphorylate exogenous substrates becomes increasingly autonomous. Withdrawal of Ca2+ after a critical level of phosphate incorporation is reached leads to a "burst" or rapid increase in Ca2+-independent autophosphorylation. The "burst" of autophosphorylation is distinct from the initial Ca2+-dependent autophosphorylation, however, since it inhibits substrate phosphorylation. Both Ca2+-dependent and Ca2+-independent substrate phosphorylation are inhibited by this autonomous autophosphorylation. Thus, autophosphorylation has a dual role in modulating the activity of multifunctional CaM kinase. It initially enables the kinase to continue phosphorylating substrates after Ca2+ levels decline, but it eventually suppresses this autonomous activity. Tryptic phosphopeptide mapping demonstrates that appearance of phosphothreonine-containing peptides is common to several conditions used to generate an autonomous enzyme. Sequencing reveals the critical "autonomy" site to be threonine286. The inhibitory mode of autophosphorylation involves 3 additional phosphopeptides containing a serine and a threonine residue. 相似文献
33.
目的 探讨全关节镜下微创复位固定术治疗SandersⅡ、Ⅲ型跟骨关节内骨折的临床疗效。方法 前瞻性随机对照研究。纳入2017年3月—2020年12月徐州仁慈医院足踝外科Sanders Ⅱ、Ⅲ型跟骨骨折患者40例,其中男36例、女4例,年龄18~58(39.6±10.8)岁,左侧22例、右侧18例,Sanders Ⅱ型16例、Ⅲ型24例。40例患者数字表法随机分为关节镜组(采用关节镜下跟骨关节内骨折微创复位、经皮螺钉固定术治疗)、大“L”形切口组(采用传统的大“L”形切口跟骨骨折复位钢板内固定术治疗),每组20例。观察指标:(1)比较2组患者性别、年龄、体质量指数(BMI)、骨折类型、术前美国足踝外科学协会(AOFAS)踝-后足功能评分等基线资料差异;(2)比较2组患者手术切口长度、手术时间、术中出血量、住院时间、术后切口愈合情况等围术期资料差异;(3)术后观察局部有无麻木感、是否钢板外露、切口皮肤坏死、术后1年是否有创伤性关节炎等手术并发症情况;(4)对比分析2组患者末次随访时AOFAS踝-后足功能评分、Gissnae角和Bohler角。结果 (1)2组患者性别、年龄、BMI、骨折类型、术前AOFAS踝-后足功能评分等基线资料比较,差异均无统计学意义(P值均>0.05)。(2)关节镜组患者的切口长度(0.94±0.08)cm、术中出血量(7.20±1.98)mL、手术时间(41.45±9.96)min、住院时间(8.45±2.01)d,大“L”形切口组患者的切口长度(14.35±1.63)cm、术中出血量(27.35±10.35)mL、手术时间(90.65±12.08)min、住院时间(17.15±6.72)d,关节镜组优于大“L”形切口组,差异均有统计学意义(t=-36.70、-8.54、-14.04、-5.54,P值均<0.001)。(3)术后平均随访12.3个月。术后关节镜组切口均甲级愈合,无创伤性关节炎、足背皮肤麻木。大“L”形切口组20例中,18例患者切口甲级愈合,2例患者切口皮缘坏死、钢板外露,予腓肠神经营养逆行岛状皮瓣修复后伤口愈合;有4例患者术后感足背外侧麻木,经治疗分别于术后8~14个月痊愈。(4)末次随访时AOFAS踝-后足功能评分关节镜组为(92.10±3.16)分、大“L”形切口组为(91.3±2.45)分,术后2组患者跟骨的Gissnae角和Bohler角均在正常范围内,组间比较差异均无统计学意义(P值均>0.05)。结论 与传统的切开复位内固定术相比较,全关节镜下微创手术治疗Sanders Ⅱ、Ⅲ型跟骨关节内骨折,具有手术切口小、手术时间短、内固定牢固、切口愈合好、疗程短、术后瘢痕不明显、功能恢复快等优势。 相似文献
34.
Sequence variation within botulinum neurotoxin serotypes impacts antibody binding and neutralization 下载免费PDF全文
Smith TJ Lou J Geren IN Forsyth CM Tsai R Laporte SL Tepp WH Bradshaw M Johnson EA Smith LA Marks JD 《Infection and immunity》2005,73(9):5450-5457
The botulinum neurotoxins (BoNTs) are category A biothreat agents which have been the focus of intensive efforts to develop vaccines and antibody-based prophylaxis and treatment. Such approaches must take into account the extensive BoNT sequence variability; the seven BoNT serotypes differ by up to 70% at the amino acid level. Here, we have analyzed 49 complete published sequences of BoNTs and show that all toxins also exhibit variability within serotypes ranging between 2.6 and 31.6%. To determine the impact of such sequence differences on immune recognition, we studied the binding and neutralization capacity of six BoNT serotype A (BoNT/A) monoclonal antibodies (MAbs) to BoNT/A1 and BoNT/A2, which differ by 10% at the amino acid level. While all six MAbs bound BoNT/A1 with high affinity, three of the six MAbs showed a marked reduction in binding affinity of 500- to more than 1,000-fold to BoNT/A2 toxin. Binding results predicted in vivo toxin neutralization; MAbs or MAb combinations that potently neutralized A1 toxin but did not bind A2 toxin had minimal neutralizing capacity for A2 toxin. This was most striking for a combination of three binding domain MAbs which together neutralized >40,000 mouse 50% lethal doses (LD(50)s) of A1 toxin but less than 500 LD(50)s of A2 toxin. Combining three MAbs which bound both A1 and A2 toxins potently neutralized both toxins. We conclude that sequence variability exists within all toxin serotypes, and this impacts monoclonal antibody binding and neutralization. Such subtype sequence variability must be accounted for when generating and evaluating diagnostic and therapeutic antibodies. 相似文献
35.
双特异性抗体对LAK细胞增殖和细胞毒作用影响的体外研究 总被引:3,自引:0,他引:3
将抗CD3与抗HBs的单克隆抗体经化学偶联得到双特异性抗体,观察该双特异性抗体对LAK细胞增殖和增强细胞毒性的作用。结果显示双特异性抗体显著提高LAK细胞与2.2.15细胞结合率;促进淋巴细胞增殖。125I-UdR释放试验检测发现双特异性抗体增强LAK细胞对2.2.15细胞的细胞毒性且与抗体浓度呈正相关。进一步对抗体的特异性研究表明,加入双特异性抗体后LAK细胞对2.2.15细胞毒性作用显著高于对照组。 相似文献
36.
Intact perceptual and conceptual priming in temporal lobe epilepsy: neuroanatomical and methodological implications 总被引:1,自引:0,他引:1
Explicit memory appears to be supported by medical temporal lobe structures, whereas separate neocortical regions may mediate perceptual and conceptual implicit memory. Children and adults with temporal lobe epilepsy (TLE) and matched controls were administered experimental verbal memory tests. Performance on implicit tests--word identification and word generation--was contrasted with explicit recognition and recall. Encoding conditions emphasized either conceptual or perceptual aspects of study words and were crossed with presentation modality. The priming performance of participants with TLE did not differ from controls, but participants with TLE did show deficits on recognition and recall measures. Thus, intact left temporal cortex does not appear to be necessary for normal implicit memory performance, even when conceptual processing is emphasized at study or test. 相似文献
37.
38.
M. Satya Murthy Edward F. Scanlon Ralph H. Silvermant Clyde R. Goodheartt Robert A. Goldschmidt Mary Lou Jelachich 《Clinical & experimental metastasis》1993,11(2):159-173
Fibronectins are a family of glycoproteins with modular functional domains. They mediate cell-cell and cell-matrix interactions which are important in embryogenesis, wound healing, metastasis and other processes. We present data on the influence of fibronectin on wound implantation of a murine mammary carcinoma line, TA3Ha. Fibronectin used in these studies was derived from bovine plasma, human serum, human foreskin fibroblasts, and mouse embryo cultures. TA3Ha cells rarely form tumors in the liver of syngeneic mice when injected intravenously but after hepatic wedge resection, 45% (107/240) of the mice develop tumors in the hepatic wound. Wound implantation is markedly reduced when the cells are pre-exposed to 200 µg/ml bovine plasma fibronectin (13%, P = 0.007), human serum fibronectin (0%, P = 0.02), human cellular fibronectin (0%, P = 0.02), or mouse cellular fibronectin (0%, P = 0.04). Lung colonization is also reduced by these fibronectins. These effects are not due to a cytotoxic action of fibronectin, since intraperitoneally injected fibronectin-treated cells form ascites tumor as effectively as do control untreated cells. Local application of a solution containing 0.25 mg/ml mouse cellular fibronectin to the hepatic wound reduces the frequency of tumor implantation from 45% to 5% (1/21, P = 0.001). No tumor implantation inhibition is seen when only suspending medium or albumin in suspending medium is used. The mechanism by which topical application of fibronectin reduces hepatic wound implantation of tumor cells is unclear, but this finding raises an exciting possibility of preventing local recurrence of cancer. 相似文献
39.
乙/丙型肝炎病毒双重感染患者前C区终止变异低频率 总被引:1,自引:0,他引:1
目的了解乙型肝炎病毒(HBV)与丙型肝炎病毒(HCV)双重感染患者前C区基因变异,及其可能的临床意义。方法用聚合酶链反应(PCR)与限制片段长度多态性(RFLP)来分析25例HBVDNA和HCVRNA均阳性(A组)和31例HBsAg和HBVDNA阳性但抗-HCV和HCVRNA均阴性(B组)的慢性肝病患者前C区密码28终止变异(终28)。结果HBV和HCV双重感染患者(A组)血清HBVDNA第1次PCR阳性率(16%)明显低于单独HBV感染组(65%)(P<0.001);前C终28检出率(28%)亦明显低于单独HBV感染(68%)(P<0.001)。结论提示双重感染患者HBV前C终止变异低频率可能与HBV低水平复制有关 相似文献
40.