Lipopolysaccharide Affects the Proliferation and Glucose Metabolism of Cervical Cancer Cells Through the FRA1/MDM2/p53 Pathway

Previous studies have found that LPS and FRA1 play opposite roles in cervical cancer. In addition, LPS functions by regulating the expression of FRA1 in many disease models, but there is currently no study of their relationship in the energy metabolism of tumor cells. This study, therefore, investigates the effects of LPS on FRA1-mediated glucose metabolism and the possible mechanisms it may have in cervical cancer cells. We constructed FRA1 stable overexpressing/ empty vector cervical cancer cell lines, where glucose consumption, the level of lactic acid production and the expression of energy metabolism related molecules were detected under the stimulation of LPS. At the same time, the changes in proliferation ability of cervical cancer cells were detected. We discovered that LPS promotes glucose consumption, lactic acid production, pentose phosphate bypass, and inhibits aerobic oxidation, by inhibiting the expression of FRA1; and that LPS promotes the growth of cervical cancer cells. Our results indicate that LPS affects the proliferation and glucose metabolism of cervical cancer cells through the FRA1/MDM2/p53 pathway.     

Applying a Psychological Nursing Care Quality Evaluation Index in hospitalized patients: A pilot study

Psychological problems have become a significant public health problem. Appropriate mental health care is crucial in promoting patient care quality. This study aimed to test the feasibility of a Psychological Nursing Quality Evaluation Index in hospitalized patients. This is a pilot study with patients hospitalized with myocardial infarction from July to September 2020 in China. The researchers used an observational approach to examine nurses′ psychological health care performance based on the Psychological Nursing Quality Evaluation Index. The results indicated high compliance rates of nurses′ psychological care performance, which provides references for evaluating and monitoring inpatient psychological nursing care.     

甘肃省武威市城市与农村不同人群碘营养及甲状腺功能对比研究

目的 分析甘肃省武威市城市和农村不同人群碘营养水平及甲状腺功能。方法 于2009年4月至2010年1月采用横断面调查方法抽取甘肃省武威市城市和农村8~10岁儿童、18~45岁成年人(不包括哺乳期妇女及孕妇)、哺乳期妇女及孕妇进行调查,采集样本人群一次随机尿样及空腹血样。砷铈催化分光光度法检测尿碘;化学发光免疫法检测血清促甲状腺激素(TSH)、游离甲状腺激素(FT₄)、游离三碘腺原氨酸(FT₃);放射免疫法检测血清甲状腺球蛋白抗体(TGAb)、甲状腺微粒体抗体(TMAb)。尿碘、TSH组间比较采用非参数检验,FT₄、FT₃组间比较采用t检验,率的比较用χ²检验。结果 甘肃省武威市城市和农村儿童、成年人、孕妇和哺乳期妇女的尿碘M(μg/L)分别为358.6、189.0、255.4、239.5和387.6、258.5、172.8、215.3,城市18~45岁成年人尿碘水平低于农村(Z=-4.020,P=0.000),孕妇尿碘水平高于农村(Z=1.424,P=0.035),差异有统计学意义。与城市相比较,农村孕妇、哺乳期妇女FT₃水平偏高(t=-3.933,P=0.000;t=-2.259,P=0.026);18~45岁成年人FT₄水平偏低(t=3.539,P=0.001)。农村孕妇TGAb、TMAb阳性率分别为43.6%、56.4%,哺乳期妇女TGAb、TMAb阳性率分别为33.3%、35.6%,均高于相应的城市人群。城市和农村不同人群甲状腺功能紊乱主要以亚甲状腺功能减退为主,不同人群各类型甲状腺功能紊乱发生率比较均无统计学意义。结论 甘肃省武威市城市、农村各人群碘营养均处于充足状态,其中儿童碘营养过剩。城市、农村成年人、孕妇和哺乳期妇女存在碘营养水平或甲状腺激素水平差异。     

医学研究生心理健康干预体系的构建

文章针对医学研究生心理健康现状及影响因素,在大数据时代背景下,基于大量的文献资料,结合心理学、教育学的相关理论,提出构建行之有效的心理健康干预体系,包括完善高校心理健康教育、优化课程体系、心理咨询与疏导、加强素质教育、减轻经济负担、获取社会支持等方式,以期改善医学研究生的心理健康状况。文章根据医学研究生心理健康特点,结合哈尔滨医科大学的具体方法,从评估对象的确定、危机干预队伍建设、预警及干预体系建立等三个方面,构建了多层次研究生心理危机干预体系,并提出了危机干预体系组织实施的主要措施、赋予导师更多责任和加强研究生自我教育,达到缓解研究生心理压力,提高研究生心理素质的目的。构建合理的心理健康干预体系对医学研究生的心理健康具有积极的作用。     

Neuropsychiatric Symptoms and Risk Factors in Mild Cognitive Impairment: A Cohort Investigation of Elderly Patients

The journal of nutrition, health & aging - Neuropsychiatric symptoms (NPS) have been shown to affect the progression and development of Alzheimer’s disease (AD) in the elderly. However,...     

肺癌热消融术后支气管胸膜瘘的研究现状与进展

【摘要】 热消融术在肺癌治疗中的耐受性良好。然而，也有一些严重的并发症。其中，支气管胸膜瘘是肺癌热消融术后罕见但最为严重的并发症之一，发生率极低，但处理困难，具有较高的致死率。早期诊断和及时正确的处理支气管胸膜瘘，是降低病死率的关键。目前关于热消融治疗肺癌安全性研究多涉及支气管胸膜瘘个例，但无系统性介绍，本文将就肺癌热消融术后支气管胸膜瘘的危险因素、临床表现、诊断和治疗进行综述。     

Effects of Boron Content on the Microstructure and Impact Toughness of 12Cr1MoVR Low-Alloy Heat-Resistant Steel Weld Metals

The impact toughness of low-Cr heat-resistant steel weld metal is an important problem to broaden the application of low-Cr heat-resistant steel. In this study, the microstructure and impact toughness of 12Cr1MoVR low-alloy heat-resistant steel weld metals with various boron contents (B1 = 0.0028%, B2 = 0.0054%, and B3 = 0.0079%) were investigated. The microstructures of all weld metals were composed of block ferrite, carbides, and inclusions. Results indicated that with increased B content, prior austenite grain sizes decreased, and minor microstructure changes could be found. With the increase in B content from 0.0028% to 0.0054% to 0.0079%, the ductile–brittle transition temperature of the weld metals decreased from 30 to 0 to −14 °C, the toughness of weld metal increased, and the hardness slightly decreased, all of which are directly related to the refinement of prior austenite grain size because of the addition of B content. However, on the top-shelf zone, such as at the testing temperature of 80 °C, ductile fracture dominates the fracture surface; with the increase in B content, the size and density of inclusions increased gradually, which led to the decrease of the impact toughness at 80 °C when the B content was 0.0079%.     

COVID-19疫情紧急状态下护理证据总结类论文的问题及对策

针对特定循证问题的证据总结为临床提供了科学依据,在当前新型冠状病毒肺炎(COVID-19)疫情紧急状态下,证据总结的作用尤为凸显。但由于疫情突发,受时间、证据数量、证据质量等因素的限制,也给证据总结的制作带来了挑战。因此,在此疫情紧急状态下,本文针对证据总结类稿件存在的问题进行分析并提出合理的对策。