The expression pattern of CD3 chain genes in fetal/maternal interface

In order to investigate the features of T-cell immune status in human placenta, the expression levels of CD3-gamma, -delta, -epsilon and -zeta chain genes in placenta were analyzed by real-time PCR. Umbilical cord blood obtained at delivery from the full-term healthy babies was used as a control. The beta2-microglobulin gene was employed as an endogenous reference, and the evaluations of mRNA expression level of each CD3 gene were used by the 2(-ΔC(t))×100% method. The expression level of CD3-gamma, -epsilon and -zeta genes (mean rank is 0.13, 0.34, and 0.49 respectively) from placenta were significantly lower than those from CB (P<0.0001). CD3-delta genes (mean rank is 5.71) expressed stronger from placenta than from CB (P = 0.0.895). Thus, the expression pattern of the four CD3 genes was presented as delta>zeta>epsilon> gamma from placenta and epsilon>gamma>zeta>delta from CB on the contrary. In conclusion, the present study characterized the expression pattern of CD3-gamma, -delta, -epsilon and -zeta chain genes from placenta, which contributes to further understanding of the features of T-cell immune status in placenta.     

应用生物型普通柄(短柄)假体治疗高龄股骨转子间骨折

目的 探讨和分析应用生物型普通柄(短柄)假体治疗高龄股骨转子间骨折的疗效.方法 回顾性分析321例中获得随访280例患者,其中男102例,女178例;年龄72 ～ 98岁,平均82.5岁.骨折类型按偏髓分型:Ⅰa型65例,Ⅰb型215例.患者多合并内科疾病,相关内科协同治疗后,对所有患者采用标准生物型普通柄假体(人工双极股骨头)置换术,术后早期进行康复锻练.结果 321例患者都顺利度过围术期,住院期间无死亡患者,术后并发症均在住院期间治愈.术后1～3周下床活动.280例获得随访,随访时间12～46个月,平均28个月,患者均能生活自理.按Harris评分标准:优129例,良121例,可22例,差8例,优良率达89.3％.结论 施行生物型普通柄人工股骨头置换治疗高龄转子间骨折,是一种有效的治疗方法,可使患者早期下床活动,减少并发症,提高患者生活质量.     

升降散对流感病毒FM1感染小鼠血清IL-1β、IL-10含量的影响

目的：观察升降散对流感病毒FM1感染小鼠炎症相关因子的影响。方法：复制流感病毒FM1感染小鼠模型,以小鼠血清IL-1β、IL-10含量为指标,用ELISA法测定。结果：升降散可以降低血清IL-1β的含量、提高血清IL-10的含量。结论：升降散调节炎症相关因子的分泌,可能是其对流感病毒FM1感染小鼠起到保护作用的机制之一。     

类风湿关节炎肾虚证大鼠模型的建立

目的：建立类风湿关节炎（rheumatoid arthritis,RA）肾虚证病证结合大鼠模型。方法：选用6周龄无特殊病原体级Sprague-Dawley大鼠110只,雌雄各半。分别采用去势法和羟基脲法制作肾虚动物模型,然后采用尾根部皮下注射Ⅱ型胶原与不完全弗氏佐剂混合物的方法诱导出关节炎。通过对大鼠一般状态观察,关节炎指数计算和关节肿胀度测量对比两种RA肾虚证病证结合动物模型的发病情况,并采用酶联免疫吸附测定法检测血浆抗Ⅱ型胶原抗体、白细胞介素6（interleukin-6,IL-6）、IL-10、γ干扰素（interferon-γ,IFN-γ）和皮质酮（corticosterone,CORT）含量,放射免疫法检测肾上腺环腺苷酸（cyclic adenylic acid,cAMP）和环鸟苷酸（cyclic guanylic acid,cGMP）含量。结果：与胶原诱导性关节炎（collagen-induced arthritis,CIA）对照组大鼠相比,结合去势法、羟基脲法制作的肾虚CIA大鼠关节红肿程度和关节损伤程度明显加重,并且这两组大鼠出现倦怠、精神萎靡,弓背蜷缩,体毛枯疏,自主活动少,反应逐渐迟钝,喜扎堆,饮食减少,大便质稀,肛周污秽等与临床肾虚证相似的症状;肾虚CIA大鼠cAMP与cGMP比值明显降低,雄性大鼠CORT含量升高,雌性大鼠的CORT水平下降,抗Ⅱ型胶原抗体含量明显升高。采用去势法制作的肾虚CIA大鼠与CIA对照组大鼠相比,IL-6、IL-10及IFN-γ水平明显升高。结论：用去势法和羟基脲法制作的肾虚证RA动物模型,既有明显的肾虚症状,又有明显的RA特征,说明这两种肾虚型RA病证结合模型能够部分地反映肾虚证RA病人的发病特点,具有可行性。而去势法制作的肾虚CIA大鼠更适合RA肾虚证的实验研究。     

弱精子症患者精子中CRISP2基因及蛋白的初步研究

目的:探讨弱精子症患者精子中富含半胱氨酸的分泌蛋白2(CRISP2)mRNA及蛋白的表达水平,探讨其与精子活力的关系及相关的分子机制。方法:收集成年男性弱精子症患者精液78例,活力正常精液70例作为对照组,50% Percoll离心分离纯化后,提取精子总RNA及总蛋白,采用RT-PCR,SYBR Green实时定量PCR和Western印迹技术检测CRISP2 mRNA及蛋白相对表达量。结果:CRISP2基因在弱精子症患者精子中的表达量明显低于其在正常对照组精子中的表达量,下降达4.3倍;Western印迹发现CRISP2蛋白在弱精子症组较正常对照组下降达1.71倍,两组之间的表达量有明显的统计学差异(P<0.05)。结论:CRISP2基因及蛋白在弱精子症患者精子中的表达下调可能与精子活力下降有密切联系,提示CRISP2基因及蛋白可能成为弱精子症发病机制研究的一个分子靶标。     

原发性腹膜后肿瘤误诊原因分析

目的分析原发性腹膜后肿瘤误诊原因,以提高其诊断率。方法回顾性分析2002年至2010年原发性腹膜后肿瘤误诊病例资料。结果资料完整共175例手术或病理确诊患者,误诊72例,误诊率为41.1%。原发性腹膜后神经鞘瘤、脂肪肉瘤常见且易误诊,误诊疾病为腹盆腔肿块、肾上腺、肾及输尿管肿瘤等。结论腹膜后肿瘤临床症状多不典型,加强对其认识、对相关临床症状的进一步分析以及必要的辅助检查可提高腹膜后肿瘤诊断成功率。     

重症脓毒症远期生活质量调查

目的 分析重症脓毒症患者出院2年到5年以后的生活质量,为脓毒症的治疗和康复提供参考.方法 采用前瞻性、观察性、双盲方法,随访2002年1月1日-2007年3月1日出院的重症脓毒症与非脓毒症外科危重病患者,根据纳入及排除标准,共362例进入随访计划,排除死亡、失访及拒绝的病例,最终51例纳入随访分析,采用SF-36量表对...     

Vinclozolin affects the interrenal system of the rare minnow (Gobiocypris rarus)

Vinclozolin, a widely used fungicide, has been characterized as a potent androgen antagonist. In this study, the effects of vinclozolin on the interrenal system of the rare minnow (Gobiocypris rarus) were evaluated. The results revealed a decline of the renal somatic index (RSI) and the presence of histopathological effects, including shrinkage of the glomerulus and expansion of the Bowman's space in the kidneys, in rare minnows exposed to vinclozolin. Elevated plasma cortisol concentrations in females exposed to ≥ 2 μg/L vinclozolin and males exposed to ≥ 10 μg/L vinclozolin (p<0.05) suggested that endocrine stress was evoked by vinclozolin exposure. Significant decreases in mRNA levels of interrenal crf, pomc, gr, and nka in females and gr and nka in males were observed after exposure to ≥ 0.5 μg/L and 2 μg/L vinclozolin (p<0.05), respectively; however, no changes in expression of these genes were observed in the brain of males (p ≥ 0.159) or females (p ≥ 0.053) compared with the control. The results indicated that female rare minnows were more sensitive than males to vinclozolin exposure. In conclusion, vinclozolin exposure evoked endocrine stress on the hypothalamic-pituitary-interrenal axis in the rare minnow, and the interrenal tissue was more sensitive than the brain tissue to stress caused by vinclozolin exposure. These results provide additional data about the modes of toxicological action of vinclozolin.     

利用文本挖掘技术探索类风湿性关节炎与冠心病异病同证的科学基础

利用已有结果进行总结是中医证候现代研究的重要内容,文本挖掘技术的应用为中医证候生物学基础的研究开辟了新的途径。"异病同证"指不同的疾病可以出现相同的证候,故可以采用相同的治疗方法,其物质基础也会有相同的地方。本研究选择类风湿性关节炎和冠心病这两种在临床上可以出现相同证候、在Medline数据库中拥有大量文献的复杂性疾病作为研究对象,采用MeSH主题词挖掘技术,对这两种疾病潜在的共性生物学网络进行探寻,进而帮助理解和研究两种疾病具有相同"证候"的可能的共性生物学基础。