活血降脂方对非酒精性脂肪肝脂质代谢及肝功能影响

目的观察活血降脂中药对非酒精性脂肪肝患者脂质代谢及肝功能的影响。方法将符合非酒精性脂肪肝诊断的126例患者,随机分为治疗组和对照组,两组患者在健康教育的基础上进行药物治疗,治疗组患者予活血降脂方煎剂口服,对照组患者予血脂康胶囊口服。两组患者疗程为3个月,比较治疗前后两组患者临床疗效总有效率,治疗前后两组总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL)与丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、γ-谷氨酰转移酶(GGT)的变化。结果两组治疗后,治疗组患者总有效率明显高于对照组,差异有统计学意义(P0.05);治疗后,两组ALT、AST、GGT较治疗前均下降,有统计学意义(P0.05);且治疗组与对照组比较差异有统计学意义(P0.05)。治疗后,两组TG、TC、LDL较治疗前均下降,有统计学意义(P0.05);治疗后治疗组TG、TC、LDL降低明显优于对照组,但无统计学意义(P0.05)。结论活血降脂方可降低非酒精性脂肪肝患者总胆固醇、甘油三酯、低密度脂蛋白,调节其脂质代谢,同时对非酒精性脂肪肝伴肝功能损害患者有明显的保肝降酶作用。     

两种鬼臼毒素制剂中鬼臼毒素含量的测定

目的:建立测定2种鬼臼毒素制剂中鬼臼毒素含量的高效液相色谱方法。方法:Dikma diamonsil C18色谱柱(4.6mm×150mm,5μm);柱温为25℃;流动相为水(A)-甲醇(B);流速1.0mL/min;梯度洗脱;检测波长为292nm。结果:鬼臼毒素在11.8-590μg/mL范围内线性关系良好(r=0.9999),疣迪搽剂和鬼臼毒素酊中鬼臼毒素的平均加样回收率分别为99.38%、99.62%。结论:文章中所建立的色谱方法快速,重现性、精密度及准确度均能满足疣迪搽剂和鬼臼毒素酊中鬼臼毒素含量测定要求。     

麝香保心丸联合辛伐他汀对冠心病心绞痛患者血清IL-18、VEGF、T-SOD水平的影响

目的研究麝香保心丸联合辛伐他汀对冠心病心绞痛患者血清白细胞介素-18(IL-18)、血管内皮生长因子(VEGF)、总超氧化物歧化酶(T-SOD)水平的影响。方法选择2018年3月—2020年8月医院收治的150例冠心病心绞痛患者为研究对象,分对照组和研究组各75例,对照组患者口服辛伐他汀,研究组在对照组基础上服用麝香保心丸,两组均连续治疗6个月,观察比较两组:(1)治疗后临床疗效及心电图疗效,(2)治疗前后中医证候积分、生化指标[IL-18、VEGF、T-SOD、甘油三酯(TG)、总胆固醇(TC)、髙密度脂蛋白(DHL)、低密度脂蛋白(LDL)、纤维蛋白原]水平、血浆黏度、心绞痛改善情况,(3)不良反应发生情况。结果治疗后,研究组临床治疗总有效率为(82.67%,62/75)和心电图总有效率(89.33%,67/75)明显高于对照组总有效率[54.67%(41/75)、69.33%(52/75)](P<0.05);治疗前,两组中医证候积分、各生化指标水平、血浆黏度、心绞痛积分、持续时间及发作频率比较差异无统计学意义(P>0.05);治疗后,两组患者中医证候积分、IL-8、L...     

Association between Excessive Dietary Branched-Chain Amino Acids Intake and Hypertension Risk in Chinese Population

The dietary intake of branched-chain amino acids (BCAAs) has been reported to be associated with both elevated blood pressure (BP) and hypertension risk, while published findings were inconsistent, and the causality has never been well disclosed. We performed this prospective study aiming to find out the relationship between dietary BCAAs intake and hypertension risk in the Chinese population. A total of 8491 participants (40,285 person-years) were selected. The levels of dietary BCAAs intake were estimated using the 24-h Food Frequency Questionnaire. Associations of both BP values and hypertension risk with per standard deviation increase of BCAAs were estimated using linear and COX regression analysis, respectively. The hazard ratios and 95% confidence interval were given. Restricted cubic spline analysis (RCS) was used to estimate the nonlinearity. Both systolic and diastolic BP values at the end points of follow-up were positively associated with dietary BCAAs intake. Positive associations between BCAAs intake and hypertension risk were shown in both men and women. By performing a RCS analysis, the nonlinear relationship between BCAAs intake and hypertension was shown. As the intake levels of Ile, Leu, and Val, respectively, exceeded 2.49 g/day, 4.91 g/day, and 2.88 g/day in men (2.16 g/day, 3.84 g/day, and 2.56 g/day in women), the hypertension risk increased. Our findings could provide some concrete evidence in the primary prevention of hypertension based on dietary interventions.     

2型糖尿病男性患者血清骨膜蛋白水平与骨质疏松的相关性研究

目的 探索骨膜蛋白水平2型糖尿病男性患者骨质疏松的关系.方法 收集了2016年3月至2017年3月在我院门诊诊断为2型糖尿病男性病人90例,体检中心健康男性人群中随机选取30例作为正常对照组,记录患者的年龄、腹围、身高、体重,计算BMI,测定甘油三酯、总胆固醇、高密度脂蛋白、低密度脂蛋白、糖化血红蛋白,血清骨膜蛋白水平...     

应用组织芯片技术研究Skp2、p27kip1在肾细胞癌中的表达及意义

目的 检测S期激酶相关蛋白2(Skp2)和抑癌基凶p27kip1在肾细胞癌(RCC)中的表达,探讨其与肾细胞癌生物学特征的关系及意义.方法 采用组织芯片技术及免疫组化SP法检测Skp2和p27kip1在80例肾细胞癌组织和40例癌旁正常肾组织中的表达,并做统计学分析.结果 (1)Skp2在肾细胞癌中的表达率高于正常肾组织(P=0.025);随肿瘤恶性程度增加,Skp2阳性表达率升高(P=0.002),其表达率与肾癌患者的年龄、性别、临床分期、淋巴结转移及肿瘤大小无关(P>0.05).(2)p27kpi1在肾细胞癌中的表达率低于正常肾组织(P=0.007);p27kip1阳性表达率与肿瘤分级及临床分期呈负相关((P<0.05),与肾癌患者的年龄、性别、淋巴结转移及肿瘤大小无关(P>0.05).(3)Skp2与p27kip1表达呈显著负相关(r=-0.273,P=0.014).结论 肾细胞癌中Skp2蛋白的过度表达与p27kip1蛋白降解增强有关,提示Skp2可能在肾细胞癌发生中扮演着重要角色.     

药物输注治疗单纯性乳管炎的临床应用

目的:探讨经溢液乳孔输注药物治疗单纯性乳导管炎的临床应用价值。方法:经临床检查、钼靶摄片及乳腺导管造影确诊为单纯性乳导管炎的惠者63例,其中乳导管扩张症52例,慢性乳导管炎11例。37例可触及质地较软的小包块。经溢液乳孔输注庆大霉素8万单位加地塞米松5mg混合液治疗。每周2次,治疗3周为一个疗程。结果:治疗1个疗程后,38例停止溢液,其中31例乳房胀痛消失;23例乳头溢液明显减少,溢液颜色变清。24例经第二个疗程治疗后(3～4次),其中20例溢液停止,18例胀痛消失,2例胀痛减轻;3例反复挤压乳头偶有少量溢液。37例包块经过治疗后30例包块消失,7例包块缩小或变软,2例效果不佳。结论:明确诊断为单纯性乳导管炎后方可行药物输注治疗,效果明显.值得临床推广运用。     

微创与开放Sweet食管切除术治疗Siewert Ⅱ型食管胃结合部腺癌的疗效比较

目的比较微创与开放Sweet食管切除术治疗Siewert Ⅱ型食管胃结合部腺癌(AEG)的疗效。方法选取2015年3月至2017年9月安徽医科大学附属省立医院收治的137例AEG患者的临床资料,根据接受手术方式的不同分为微创Sweet食管切除术(MISE)组38例,开放Sweet食管切除术(OSE)组99例。比较两组术中指标及术后疗效情况。结果与OSE组相比,MISE组术中出血量较少,手术时间更长,淋巴结清扫总数及腹腔淋巴结清扫数更多,差异有统计学意义(P <0.05)。两组共有45例(32.85%)患者术后发生2级以上并发症,均以肺部感染为最常见; 4级以上严重并发症主要为严重吻合口瘘和呼吸衰竭。OSE组2～3级并发症发生率高于MISE组,差异有统计学意义(P <0.05)。MISE组和OSE组的1年生存率分别为92.1%和87.9%,3年生存率分别为68.2%和59.6%,两组术后生存情况比较差异无统计学意义(P=0.383)。两组术后复发、转移的发生情况比较差异无统计学意义(P>0.05)。Cox回归分析结果显示,以N₀期为参考,N     

热应激通过激活NLRP3/IL-1β/IL-6途径介导肝脏炎症损伤效应

目的 探究热应激致肝炎症损伤的分子机制.方法 雌性SD大鼠随机分为对照组和热应激组,38℃暴露建立热应激动物模型,每天暴露2 h,连续3 d,分离肝组织;体外培养的L-02细胞分为对照组、热应激组、热应激+二甲基亚砜(DMSO)组及热应激+白藜芦醇组(100μmol/L),采用5％CO2,41℃暴露建立热应激细胞模型,...     

First-Principles Study on the Adsorption Behavior of O2 on the Surface of Plutonium Gallium System

To deeply understand the adsorption process of oxygen on the surface of a plutonium gallium system and to reveal the chemical reaction mechanism at the initial stage of oxidative corrosion on the surface of plutonium gallium alloy at a theoretical level, the adsorption behavior of oxygen molecules on the surface of a plutonium gallium system was investigated by a first-principles approach based on density flooding theory. The results show that the molecular bond length increases and finally breaks when the surface oxygen molecule is adsorbed on the surface of plutonium gallium system and dissociates into two atomic states. The most likely adsorption position of oxygen molecules on the surface of plutonium gallium system is hole-site vertical adsorption with the adsorption energy size of 10.7 eV. The bonding between oxygen atom and surface is mainly due to the overlapping hybridization of Pu-6s, Pu-7s, Pu-6d, Ga-3d and O-2p orbitals. Oxygen molecules mainly interact with the atoms of the first layer on the surface of the plutonium gallium system. The oxygen atoms after stable adsorption are able to diffuse to the subsurface of the plutonium gallium system after overcoming the energy barrier of 16.7 eV and form a stable structure. The research results reveal the initial reaction process and adsorption law of oxygen on the surface of plutonium gallium system from microscopic level, which is helpful to further explore the surface corrosion prevention technology of plutonium gallium system and improve the reliability and safety of plutonium gallium alloy components.