上皮根鞘细胞在牙骨质发育中的作用

目的：观察牙根发育中上皮根鞘细胞（Hertwig＇s epithelial root sheath,HERS）断裂后结局和增殖凋亡相关基因的表达,探讨上皮根鞘在牙根发育特别是牙骨质形成中的可能作用.方法：用TUNEL（ TdT- mediated - dUTP nick end labeling , TUNEL） 法原位细胞凋亡检测牙根发育中上皮根鞘细胞凋亡的情况.以细胞角蛋白14（cytokeratin 14）标记上皮根鞘细胞,追踪其断裂后结局.SP免疫组化法检测增殖细胞核抗原（proliferating cell nuclear antigen, PCNA ）和凋亡抑制基因Bcl-2在上皮根鞘细胞的表达.结果：牙冠形成后,内外釉上皮结合增殖形成HERS,此时呈PCNA表达阳性;牙根发育开始后,HERS呈Bcl-2阴性表达,在少量牙本质基质形成处,上皮根鞘细胞开始呈TUNEL阳性表达,之后HERS断裂,在其外侧可见成牙骨质细胞的形成,断裂的HERS细胞部分转入牙周膜形成上皮剩余（the epithelial rest of Malassez）,而细胞牙骨质形成中可见部分埋入细胞呈CK14阳性表达.结论： HERS细胞的凋亡可能会为成牙骨质细胞分化提供信号,部分HERS细胞可能参与牙骨质的形成.     

A multifunctional, provisional, implant-retained fixed partial denture

This article describes the modification of a procedure for fabricating a laboratory-processed, metal-reinforced, acrylic resin provisional restoration that becomes an implant-retained fixed partial denture. The modification involves the incorporation of patrix and matrix components into a cast metal framework. The prosthesis can be used as an alternative to a removable radiologic stent and surgical guide. It can function as a surgical guide during implant placement and help retract the buccal mucogingival flap during implant placement. The prosthesis also can be used as an aid in locating the implant during stage II surgery. Finally, the pontics can be converted into an implant-supported provisional restoration immediately after the implant prosthetic components are attached to the uncovered implants.     

A Profile of Black and Hispanic Subgroups'' Access to Dental Care: Findings from the National Health Interview Survey

This study examined which black and Hispanic minority subgroups were least likely to obtain dental care and why, based on logistic regression analyses of the 1986 National Health Interview Survey. Blacks and Hispanics were less apt to have private dental insurance coverage, to be knowledgeable about the purpose of fluoride, to have been to a dentist in the past year, and, when they did go, were more apt to have gone in response to symptoms rather than for preventive reasons, compared to whites. Logistic regression analyses for adults 18 years of age and older and for children and adolescents 2 to 17 years of age showed that the following individuals had the lowest probability of having been to a dentist in the past year: males, members of larger families, adults who were unemployed or in blue-collar jobs, those who lived in the South or nonmetropolitan areas, people who perceived their health to be fair or poor, and those with no private dental insurance. Mexican-Americans were least likely to have been to a dentist regardless of their income or education. In general, the findings confirmed the importance of dental insurance, as well as suggesting a need for more school-based dental programs and public health clinic-based dental health education and outreach efforts for targeting minority children and adults.     

Immunohistochemical localization of CEA,EMA and keratin in salivary mucoepidermal carcinoma]

Localizations of CEA,EMA and keration in 19 cases of mucoepidermal carcinomas were investigated using the indirect immunoperoxidase technique.The results showed CEA was negative in normal salivary glands and showed faint reaction in glands near carcinoma tissue.Keratin and EMA were localized in some myoepithelial cells.The positive rates in carcinoma tissue were 78.9%,89.5% and 84.2%,respectively.The positive rates and staining intensity of CEA and EMA in carcinoma tissue gradually decreased with the decline of tumor differentitation,but that of keratin showed no variation.the author consider that CEA and EMA could become good indices in clinically diagnosing mucoepithelial carcinoma and determining tumor differentiation type cell in mucoepidermal carcinoma and have a potential to multiply express the tumor elements of epithelium and/or mesenchyma.     

妇科肿瘤患者化疗期症状群与生活质量的动态变化及其相关性研究

目的 分析妇科肿瘤患者化疗期不同时间点的症状群与生活质量的动态变化,并探讨两者间的相关性.方法 采用前瞻性研究方法,以137例妇科肿瘤化疗患者为研究对象,使用一般资料问卷、中文版安德森症状评估量表(MDASI-C)和欧洲癌症研究与治疗组织(EORTC)生活质量问卷(QLQ-C30)量表在化疗后即刻(T1)、化疗后1个月(T2)、化疗后3个月(T3)及化疗后6个月(T4)4个不同时间点进行动态调查.结果 妇科肿瘤患者在化疗期4个时间点的症状严重程度(F=76.31,P=0.00)、生活质量(F=117.65,P=0.00)均有差异.T3时间点的症状最严重、生活质量最差.T1时间点提取出4个症状群,分别为:病感症状群、消化道症状群、神经系统症状群及疼痛相关症状群,与生活质量多个领域得分呈正相关(P<0.05);T3时间点提取出5个症状群,分别为:神经系统症状群、病感症状群、疼痛相关症状群、情感症状群及消化道症状群.以上症状群与QLQ-C30量表多个领域得分呈正相关(P<0.05).结论 在化疗后即刻至化疗后3个月,妇科肿瘤患者化疗期间的症状群越来越严重,生活质量越来越差.     

Inverted U-Shaped Associations between Glycemic Indices and Serum Uric Acid Levels in the General Chinese Population: Findings from the China Cardiometabolic Disease and Cancer Cohort (4C) Study

Objective The relationship between serum uric acid(SUA)levels and glycemic indices,including plasma glucose(FPG),2-hour postload glucose(2 h-PG),and glycated hemoglobin(HbA1 c),remains inconclusive.We aimed to explore the associations between glycemic indices and SUA levels in the general Chinese population.Methods The current study was a cross-sectional analysis using the first follow-up survey data from The China Cardiometabolic Disease and Cancer Cohort Study.A total of 105,922 community-dwelling adults aged≥40 years underwent the oral glucose tolerance test and uric acid assessment.The nonlinear relationships between glycemic indices and SUA levels were explored using generalized additive models.Results A total of 30,941 men and 62,361 women were eligible for the current analysis.Generalized additive models verified the inverted U-shaped association between glycemic indices and SUA levels,but with different inflection points in men and women.The thresholds for FPG,2 h-PG,and HbA1 c for men and women were 6.5/8.0 mmol/L,11.0/14.0 mmol/L,and 6.1/6.5,respectively(SUA levels increased with increasing glycemic indices before the inflection points and then eventually decreased with further increases in the glycemic indices).Conclusion An inverted U-shaped association was observed between major glycemic indices and uric acid levels in both sexes,while the inflection points were reached earlier in men than in women.     

Comparative Study of the Genetic Diversity,Antimicrobial Resistance,and Pathogenicity of Aeromonas Isolates from Clinical Patients and Healthy Individuals

Objective This study was performed to compare the genetic diversity, virulence, and antimicrobial resistance of Aeromonas strains isolated from patients and healthy individuals. Methods A total of 38 clinical strains and 19 strains from healthy individuals were isolated from the samples collected in Ma'anshan City, Anhui Province. Their taxonomy was investigated using concatenated gyrB-cpn60 sequences, and their resistance to 12 antibiotics was evaluated. The pathogenicity of these strains was examined through beta-hemolysis, protease activity, and virulence gene assays. Results The 57 Aeromonas strains were divided into 55 sequence types. Of these types, 21 were novel, suggesting that their genetic diversity was high. These Aeromonas isolates could be divided into 7 species, and the positive rates of beta-hemolysis and protease activity were 49.1％ and 73.7％, respectively. The detection rate of clinical patients in terms of beta-hemolysis and protease activity was higher than that of healthy individuals. Among the four most common Aeromonas strains, A. dhakensis had the highest detection rate of virulence genes. The multidrug resistance rate of the clinical isolates was much higher than that of the strains isolated from healthy individuals. Conclusions The taxonomy, virulence properties, and antibiotic resistance of Aeromonas isolates from patients differ from those of the isolates from healthy individuals.     

A Risk Prediction Model for Ischemic Stroke in Southern Chinese Population: Impact of Multiple Genetic Variants and Clinical/Lifestyle Factors

With a global prevalence of 7.7 million, ischemic stroke (IS) is one of the leading causes of death and disability worldwide. In China, IS alone contributed to 69.6％? of stroke events and accounted for 37.1％? of the mortality/disability rate[1]. IS is a complex disease that is known to be associated?with?various?genetic?variants?and?clinical and lifestyle risk factors[2]. Genome -wide association studies (GWAS) provided evidence for the?occurrence?of?more?than?160?IS-associated?single nucleotide?polymorphisms?(SNPs).?In?terms?of?clinical risk factors, patients with a history of chronic diseases like hypertension, dyslipidemia, and diabetes mellitus, display a higher risk of developing IS. Among the various lifestyle components, leading a sedentary lifestyle, smoking, and having an unhealthy diet are known to be associated with the risk of IS. Thus, integration of various genetic, clinical, and lifestyle variables might prove to be highly beneficial in the prediction and prevention of IS?at?the?individual?level[3].