Rat cerebellar cells in tissue culture. I. An immunological marker for brain vascular endothelial cells (antibodies from the sera of patients with Chagas' disease)

Immunohistochemical studies showed that immunoglobulins from sera of patients with Chagas' disease bind specifically to endothelial cells in cryostat sections of the rodent brain. In monolayer cultures of 8-day-old rat cerebella both surface and internal labelling of the endothelial cells was detected. The contrast immune sera raised against cold-insoluble globulin (which cross-reacts with fibronectin) also labelled the surface of fibroblasts as well as the endothelial cells. It would appear that Chagasic immunoglobulins are a useful marker for endothelial cells in neural cell culture.     

Thoracic disk herniation: MR imaging

The authors undertook a retrospective study to assess the role of magnetic resonance (MR) imaging in thoracic disk herniation. The initial MR images were interpreted independently of other findings. These interpretations were compared with the findings of plain and computed tomography (CT) myelography and surgery, when available. Sixteen thoracic disk herniations were confirmed with plain and CT myelography and/or surgery. Plain myelography was performed on 14 patients and showed focal ventral filling defects in nine. Results of CT myelography were equivalent to those of MR imaging with three pulse sequences (sagittal T1 and T2 weighted, axial T1 weighted) in the identification of all the abnormal levels. In two patients, the signal from the herniated disk was so low on all sequences that thoracic disk herniation had to be inferred from the mass effect on the spinal cord. Precise location of the abnormal level with body coil MR images was achieved in six of 13 patients.     

Placental electron microscopy and histochemistry in a case of sialic acid storage disorder

Morphological alterations in a placenta from a case of infantile sialic acid storage disease are described. Syncytiotrophoblast, villous capillary endothelial cells, amniotic and Hofbaüer cells were filled with membrane-bound inclusions which were either electron-lucent or contained fibrillogranular material. Hydrolysis of slides with neuraminidase demonstrated at six hours Alcian blue material which was not present in normal syncytium. The possibility that a storage disorder such as sialic acid storage disease can be accurately diagnosed by electron microscopy on chorion villous sampling at nine to ten weeks is emphasized.     

Bioequivalence of Topical Dermatological Dosage Forms-Methods of Evaluation of Bioequivalence

Pharmaceutical Research -