全文获取类型
收费全文 | 390篇 |
免费 | 17篇 |
国内免费 | 14篇 |
专业分类
儿科学 | 17篇 |
妇产科学 | 14篇 |
基础医学 | 41篇 |
口腔科学 | 9篇 |
临床医学 | 30篇 |
内科学 | 91篇 |
皮肤病学 | 6篇 |
神经病学 | 4篇 |
特种医学 | 132篇 |
外科学 | 28篇 |
综合类 | 7篇 |
预防医学 | 15篇 |
眼科学 | 2篇 |
药学 | 17篇 |
肿瘤学 | 8篇 |
出版年
2019年 | 2篇 |
2018年 | 5篇 |
2017年 | 1篇 |
2016年 | 4篇 |
2015年 | 8篇 |
2014年 | 14篇 |
2013年 | 11篇 |
2012年 | 8篇 |
2011年 | 13篇 |
2010年 | 9篇 |
2009年 | 17篇 |
2008年 | 12篇 |
2007年 | 11篇 |
2006年 | 6篇 |
2005年 | 7篇 |
2004年 | 4篇 |
2003年 | 3篇 |
2002年 | 3篇 |
2001年 | 4篇 |
2000年 | 3篇 |
1999年 | 8篇 |
1998年 | 20篇 |
1997年 | 23篇 |
1996年 | 22篇 |
1995年 | 10篇 |
1994年 | 16篇 |
1993年 | 7篇 |
1992年 | 7篇 |
1991年 | 1篇 |
1990年 | 7篇 |
1989年 | 12篇 |
1988年 | 9篇 |
1987年 | 10篇 |
1986年 | 12篇 |
1985年 | 14篇 |
1984年 | 6篇 |
1983年 | 17篇 |
1982年 | 19篇 |
1981年 | 15篇 |
1980年 | 10篇 |
1979年 | 5篇 |
1978年 | 6篇 |
1977年 | 11篇 |
1976年 | 6篇 |
1975年 | 1篇 |
1973年 | 2篇 |
排序方式: 共有421条查询结果,搜索用时 46 毫秒
71.
72.
73.
74.
We have examined the morphological and secretory behavior of rat and guinea pig megakaryocytes exposed for up to 24 hours to extracellular matrix produced by cultured bovine endothelial cells. By phase-contrast microscopy of living cells and in more detail by scanning electron microscopy, the megakaryocytes showed a nonreversible adherence, an extensive formation of filopodia around the periphery like the rays of the sun, and a tendency toward flattening. These filopodia were generally linear with attenuated tips and were larger than, but resembled the filopodia of, rat or guinea pig platelets exposed to this extracellular matrix. In contrast, isolated megakaryocytes on glass or on uncoated plastic surfaces did not show these responses; adherence, in the face of gentle agitation before fixation, was minimal, with rare filopodia and no flattening. Megakaryocytes that interacted with the extracellular matrix produced significant amounts of thromboxane A2, but this did not occur on uncoated surfaces and could not be attributed to other contaminating cells in the megakaryocyte suspensions. The appearance in megakaryocytes of these typical platelet responses indicates that megakaryocytes acquire the functional capabilities of platelets by the synthesis and assembly of platelet substances and organelles. Thromboxane production by megakaryocytes stimulated by the extracellular matrix is a readily quantifiable measure of this capacity. 相似文献
75.
Van Santen S de Mast Q Luty AJ Wiegerinck ET Van der Ven AJ Swinkels DW 《The American journal of tropical medicine and hygiene》2011,84(1):148-151
In malaria-endemic areas, iron deficiency and placental Plasmodium falciparum infection commonly coexist. In primigravidae and their newborns, hepcidin and other iron parameters were evaluated in groups and classified according to placental P. falciparum and maternal anemia status. Mothers had relatively high hepcidin levels considering their low iron status. In cord blood, levels of hepcidin, hemoglobin, and other iron parameters were also similar for groups. We conclude that maternal hepcidin is not significantly altered as a function of placental infection and/or anemia. Importantly, fetal hemoglobin and iron status were also unaffected, regardless of the presence of placental infection or maternal anemia. 相似文献
76.
C. N. van Dijk M. N. van Sterkenburg J. I. Wiegerinck J. Karlsson N. Maffulli 《Knee surgery, sports traumatology, arthroscopy》2011,19(5):835-841
The terminology of Achilles tendon pathology has become inconsistent and confusing throughout the years. For proper research,
assessment and treatment, a uniform and clear terminology is necessary. A new terminology is proposed; the definitions hereof
encompass the anatomic location, symptoms, clinical findings and histopathology. It comprises the following definitions: Mid-portion Achilles tendinopathy: a clinical syndrome characterized by a combination of pain, swelling and impaired performance. It includes, but is not limited
to, the histopathological diagnosis of tendinosis. Achilles paratendinopathy: an acute or chronic inflammation and/or degeneration of the thin membrane around the Achilles tendon. There are clear distinctions between acute
paratendinopathy and chronic paratendinopathy, both in symptoms as in histopathology. Insertional Achilles tendinopathy: located at the insertion of the Achilles tendon onto the calcaneus, bone spurs and calcifications in the tendon proper at
the insertion site may exist. Retrocalcaneal bursitis: an inflammation of the bursa in the recess between the anterior inferior side of the Achilles tendon and the posterosuperior
aspect of the calcaneus (retrocalcaneal recess). Superficial calcaneal bursitis: inflammation of the bursa located between a calcaneal prominence or the Achilles tendon and the skin. Finally, it is suggested
that previous terms as Haglund’s disease; Haglund’s syndrome; Haglund’s deformity; pump bump (calcaneus altus; high prow heels;
knobbly heels; cucumber heel), are no longer used. 相似文献
77.
Rob F. Wiegerinck Jacques M. T. de Bakker Tobias Opthof Nicolaas de Jonge Hans Kirkels Francien J. G. Wilms-Schopman Ruben Coronel 《Basic research in cardiology》2009,104(3):321-332
Aim To investigate ventricular conduction and refractoriness before and after application of rotigaptide, an enhancer of gap junctional
conductance, to explanted hearts of patients with heart failure (HF).
Methods and results In six explanted perfused hearts of patients with end-stage HF, activation/repolarization mapping was performed and refractory
periods (RPs) and activation recovery intervals (ARIs) were measured before and after application of 50 nM rotigaptide. Rotigaptide
caused a decrease of RP from 476 ± 36 to 453 ± 31 ms (P < 0.05), but did not change ARI-dispersion. During premature activation along the fibers rotigaptide decreased the minimal
activation time (ATmin) and maximal activation time (ATmax) significantly from 35 ± 12 to 24 ± 9 and from 97 ± 38 to 43 ± 7 ms, respectively. Rotigaptide did not change ATmin and ATmax during activation perpendicular to the fiber direction. After application of rotigaptide conduction curves normalized in
five/six recordings when activation was parallel, but destabilized in three/six hearts when activation was perpendicular to
fiber direction. The destabilization was associated with local conduction delays rather than with facilitation of conduction.
Conclusion Rotigaptide applied to hearts of patients with end-stage HF shortened RPs normalized conduction curves and increased conduction
parallel to fiber direction. However, in 50% of the hearts local slowing of conduction with destabilization of conduction
(curves) occurs at sites close to the stimulation site, when activation is perpendicular to fiber direction.
Returned for 1. Revision: 14 July 2008 1. Revision received: 26 August 2008
Returned for 2. Revision: 19 September 2008 2. Revision received: 31 October 2008
Returned for 3. Revision: 17 November 2008 3. Revision received: 18 November 2008 相似文献
78.
79.
Fávaro WJ Nunes OS Seiva FR Nunes IS Woolhiser LK Durán N Lenaerts AJ 《Infectious agents and cancer》2012,7(1):14-15
Background
Compounds that can act as agonists for toll-like receptors (TLRs) may be promising candidates for the development of drugs against infectious diseases and cancer. The present study aimed to characterize the immunomodulatory effects of P-MAPA on TLRs in vitro and in vivo, as well as to investigate its potential as adjuvant therapy in infectious diseases and cancer.Methods
For these purposes, the activity of P-MAPA on TLRs was assayed in vitro through NF-??B activation in HEK293 cells expressing a given TLR, and using an in vivo animal model for bladder cancer (BC). The antimicrobial activity of P-MAPA was tested against Mycobacterium tuberculosis (TB) in vitro in an MIC assay, and in vivo using an aerosol infection model of murine tuberculosis. Antitumor effects of P-MAPA were tested in an animal model with experimentally induced BC. Moxifloxacin (MXF) and Bacillus Calmette-Guerin (BCG) were used as positive controls in the animal models.Results
The results showed that P-MAPA, administered alone or in combination with MXF, induced significant responses in vivo against TB. In contrast, the compound did not show antimicrobial activity in vitro. P-MAPA showed a significant stimulatory effect on human TLR2 and TLR4 in vitro. In BC, TLR2, TLR4 and p53 protein levels were significantly higher in the P-MAPA group than in the BCG group. The most common histopathological changes in each group were papillary carcinoma in BC group, low-grade intraepithelial neoplasia in BCG group and simple hyperplasia in P-MAPA group. Concerning the toxicological analysis performed during BC treatment, P-MAPA did not show evidence for hepatotoxicity and nephrotoxicity.Conclusions
In conclusion, P-MAPA acted as TLR ligand in vitro and improved the immunological status in BC, increasing TLR2 and TLR4 protein levels. P-MAPA immunotherapy was more effective in restoring p53 and TLRs reactivities and showed significantly greater antitumor activity than BCG. The activation of TLRs and p53 may provide a hypothetical mechanism for the therapeutic effects in both cancer and infectious diseases. Taken together data obtained will encourage the further investigation of P-MAPA as a potential candidate for the treatment of cancer and infectious diseases. 相似文献80.